복막염을 유발한 쥐의 복막에서 혈관내피 성장인자의 발현

채은영(Eun Young Chae),박주현(Joo Hyun Park),진동찬(Dong Chan Jin),김용수(Yong Soo Kim),장윤식(Yoon Sik Chang),방병기(Byung Kee Bang) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.3

목 적: 복막투석 환자에서 복막염이 발생하면 복막의 투과성이 항진되어 한외여과 장애가 발생하는데 복막염이 발생했을 때 복막의 투과성이 항진되는 기전에 대하여는 아직 자세히 밝혀지지 않고 있다. 본 연구는 쥐에서 복막염을 유발한 후 복막 투과성의 변화, 복막 조직 소견의 변화 및 복막에서 강력하게 혈관 투과성을 항진시키는 성장인자인 혈관내피 성장인자(Vascular endothelial growth factor, VEGF)의 발현을 규명하고자 시행하였다. 방 법 : Sprague-Dawley 쥐를 대상으로 복막 투석 도관을 삽입한 후 복막 평형검사를 시행하고 대조군(7마리)과 복막염군(13마리)으로 분류하여 복막염군에는 Staphylococcus aureus균 을 1×10 exp (9) colony forming unit 복강내로 주입하여 복막염을 유발한 후 5일째 다시 복막 평형 검사를 시행하고 쥐를 희생하여 복막조직을 생검하였다. 복막 평형검사는 쥐의 혈청을 0시간에 (P_0), 투석액 배액을 0시간(D_0)과 2시간(D_2)에 채취하여 포도당(D_2/D_0)과 총 단백질(D_0 /P_0 및 D_2/P_0)의 투과율을 계산하였고 복막 조직은 PAS 염색과 대식세포 및 혈관내피 성장인자에 대한 면역조직염색을 시행하였다. 결 과 : 복막 평형검사 결과 포도당의 투석액내 농도비는(D_2/D_0) 복막염 유발 5일 후 대조군에 비하여 복막염군에서 유의하게 낮았고 총 단백질의 투석액/혈장 농도비(D2/P0)도 복막염 유발 5일 후에는 대조군에 비하여 복막염군에서 유의하게 높아 저분자 물질인 포도당과 고분자 물질인 단백질의 복막 투과율 모두 증가되었다. 복막조직은 복막염군에서 복막 중피세포 하층에 염증세포의 침윤이 증가되고 복막 중피세포 하층이 두꺼워졌으며 중피세포의 탈락현상도 보였다. 대조군은 대식세포의 침윤이 거의 없었으나 복막염군은 복막 중피세포 하층에 대식세포의 침윤이 현저히 증가되었고 혈관내피 성장인자는 대조군에서 복막 중피세포 층에서 약하게 염색되었으나 복막염군에서는 복막 중피세포층에서 강하게 염색되고 복막 중피세포 하층에 침윤된 대식 세포에서도 강하게 염색되었다. 결 론 : 복막염을 유발한 쥐는 복막의 투과율이 유의하게 증가되었고 그 기전에는 복막 중피세포와 복막 중피세포 하층에 침윤된 대식세포에서 혈관내피 성장인자 발현의 증가가 관여할 것으로 생각된다. Background : Ultrafiltration failure resulting from increased peritoneal vascular permeability during peritonitis is a major problem in CAPD patients. However, the mechanism of increased peritoneal permeability during peritonitis has not been clearly determined. We studied changes in the peritoneal permeability and the expression of vascular endothelial growth factor(VEGF), which is known to increase vascular permeability, in the peritoneal tissues of rats with peritonitis. Methods : After implanting peritoneal dialysis catheter to 20 Sprague-Dawley rats and performing peritoneal equilibration test(PET), rats were divided into control group(n=7) and peritonitis group(n=13). One ml of saline or Staphylococcus aureus(1×10 exp (9) colony forming unit/mL) was injected intraperitoneally to control or peritonitis group for five days, and PET was repeated. Peritoneal transport rates of glucose and total protein were measured, and macrophages infiltration and VEGF expression in peritoneal tissues were examined by immunohistochemical stain. Results : Peritoneal transport rates of glucose and total protein were significantly increased in peritonitis group compared with control group, suggesting that peritonitis increased peritoneal transport rates of both low and high molecular weight solutes. The peritoneal tissues of peritonitis rats showed profuse infiltration of macrophages in the submesothelial space, submesothelial widening and denudation of mesothelial cells. While VEGF was slightly expressed in peritoneal mesothelial cells in control rats, it was intensively stained not only in the peritoneal mesothelial cells but also in the infiltrated macrophages in the submesothelial space in peritonitis rats. Conclusion : Peritonitis increases peritoneal vascular permeability and VEGF expression in peritoneal mesothelial cells and infiltrated macrophages in the submesothelial space. These data suggest that VEGF may play a role in the increased peritoneal permeability during peritonitis.

한국놀이치료학회지 연구동향 - 학회지 창간부터 2017년까지

채은영 ( Chae Eun-young ),정문주 ( Cheong Moon-joo ) 한국놀이치료학회 2017 한국놀이치료학회지(놀이치료연구) Vol.20 No.4

본 연구는 “한국놀이치료학회지(놀이치료연구)”에 1998년부터 2017년 9월까지 게재된 총 371편의 논문들에 대한 분석을 실시하였다. 구체적으로는 논문 발행 현황 및 연구자 특성, 연구 유형, 연구 내용, 연구 대상자, 연구 영역, 놀이치료 사용과 방법, 자료 분석 방법, 기타 인구통계학적 분석에 초점을 맞추었다. 그 결과, 학회지의 영향력 지수는 1.65로 타 치료 관련 학회지보다 높게 나타났다. 연구 유형은 전체 371편 중 양적 연구가 63.3%로 가장 높게 나타났으나, 2006년을 기준으로 질적 연구가 꾸준히 증가한 것으로 나타났다. 연구 내용으로는 연구대상의 지각 및 태도와 관련된 연구가 가장 많았고, 연구 대상으로는 초등학생, 상담자 관련 연구의 순서로 많이 진행되고 있다. 연구 영역은 다양한 영역에서 이루어지고 있었는데, 연구대상자의 인지와 관련된 연구의 비중이 높았다. 사용되는 놀이치료 종류와 방법도 특정 기법보다는 놀이치료라는 큰 범주 안에서 연구되고 있었다. 양적 연구의 데이터분석 방법은 기술통계, 변량분석, 매개효과 등의 회귀 분석순으로 많이 사용되었다. 또한 질적 연구 분석 방법에서는 사례연구가 반을 차지하였다. 기타 사례연구 중에서는 남아 대상 연구가 많았으며, 2인 저자의 비율이 가장 높게 나타났다. 이러한 결과들을 통해 연구의 의미와 학회지에 주는 시사점에 대해 논의하였다. The purpose of this study was to analyze 371 papers from 1998 to September 2017 in the Korean Journal of Play Therapy. More specifically, it focused on the publishing status, researcher characteristics, research type, research content, subject, research area, use and methods of play therapy, the data analysis method, and other methods of demographic analysis. Our result determined the influence index of the journal was 1.65 which was higher than that of other journals. The quantitative research of the research types was the highest among the 371 items (63.3%), however the amount of qualitative research steadily increased in 2006. The results of this study are as follows. First, research on the perception and attitudes of the research subjects was the most common type of research. Study areas were composed of various areas, and the percentile of the study subjects' perception area was the highest. The types and methods of play therapy have also been studied within the larger category of play therapy rather than as specific techniques. Data analysis methods commonly used in quantitative research were used in the following order of descriptive statistics, variance analysis, and median effects. In terms of the qualitative research analysis method, half of the case studies were used. Among the other case studies, there were many studies on boys, and the ratio of two authors was the highest. Through these results, The implications of these results on future research and the implications for this journal were also discussed.

저구연산뇨증에 의한 신결석과 신석회화증을 동반한 원위세관 산증 1 예

채은영(Eun Young Chae),장윤식(Yoon Sik Chang),신동일(Dong Il Shin),태현정(Hyun Jung Tae),박용범(Yong Bum Park),박선희(Sun Hee Park) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.3

Renal stone and nephrocalcinosis are common clinical manifestations of type 1 renal tubular acidosis. In normal state, citrate plays the most critical role in suppressing stone formation as it combines with calcium. In type 1 RTA, increased reabsorption of citrate in proximal tubule results in low citrate excretion, which precipitates renal stone formation. We report a case of type 1 RTA accompanying renal stone and nephrocalcinosis caused by hypocitraturia. A 16-year-old male patient who had renal stone and nephrocalcinosis showed hypocitraturia. Incomplete type 1 RTA was proved as the cause of hypocitraturia by bicarbonate and ammonium loading test in the patient.

부모와 교사의 자율성 지지, 아동의 기본심리욕구, 아동의 의사결정 참여 간의 구조적 관계

채은영(Eun Young Chae),이재연(Jae Yeon Lee) 한국아동학회 2017 아동학회지 Vol.38 No.1

Objective: The purpose of this study was to explore the structural relationships among parents’ and teachers’ autonomy support, children’s basic psychological needs, and children’s participation in decision-making. Methods: The participants were 687 elementary school students (5th and 6th grades) in Seoul and Gyeonggi-do. The data were analyzed using SEM. Results: First, parents’ and teachers’ autonomy support had a direct effect on children’s participation in decision-making. Second, parents’ and teachers’ autonomy support had an indirect effect on children’s participation in decision–making through the children’s basic psychological needs. Third, children’s basic psychological needs mediated the effects of parents’ and teacherss autonomy support and children’s participation in decision-making. Conclusion: The findings suggest the importance of the mediating effect of children’s basic psychological needs, between parents’ and teachers’ autonomy support and children’s participation in decision-making. The findings can be used as an important basis for prospective research and practices for improving children’s participation in decision-making.

황연(黃連)이 Cationic Bovine Serum Albumin 투여로 유발된 Membranous Nephropathy Mouse Model에 미치는 영향

채은영 ( Eun Young Chae ),조충식 ( Chung Sik Cho ),김철중 ( Cheol Jung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.1

Objectives: The current treatment regimens for patients with nephrotic syndrome due to membranous nephropathy(MN) are based on steroids or immunosuppressive therapy with the aim of reducing proteinuria and improving outcome. Although these treatments attenuate the deterioration of renal function in MN patients, it has been suggested that all are burdened by significant toxicity. Therefore, more specific and less toxic therapies are needed. This study was to evaluate the effects of Coptidis Rhizoma Extract(CRE) on the MN induced by cBSA in mice. Methods: Mice were divided into 4 groups. One group named for `Normal` was injected with a saline solution not to be immunized. The rest groups were treated as follows; After mice were immunized with 0.2 ㎎ of cBSA and Freund`s complete adjuvant one time every two weeks for 6 weeks, they received intra-peritoneal injection of 10 ㎎/㎏ of cBSA daily for 4 weeks. Also, they were divided into 3 groups. The first named for `Control` was not given CRE. The second for `CRE-250` was given oral administration of 250 ㎎/㎏ of CRE daily for 4 weeks. The third for `CRE-500` was given 500 ㎎/㎏ of CRE. All of mice were sacrificed 4 weeks after the first immunization. We measured a body weight and 24hrs proteinuria as well as serological analysis. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results: The levels of 24 hrs proteinuria, triglyceride, IgG, IL-6 were significantly decreased in both CRE groups. And the level of IgM was significantly decreased in CRE-250 group. In histological findings of kidney tissue, thickening of GBM and deposition of electron-density were consideraly decreased in both CRE groups. Conclusions: The present study suggests that CRE is highly effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.

아동의 기본심리욕구와 관련 요인에 대한 메타분석

채은영(Eun Young Chae),정문주(Moon Joo Cheong) 한국아동학회 2016 아동학회지 Vol.37 No.4

Objective: The purpose of this study was to analyze correlation effect size between Basic Psychological Needs (BPN) and factors affecting BPN. Methods: This research was reviewed and synthesized systematically by meta-analyse. Fifty-eight published studies between 2008 and 2016 were sampled and the effect sizes were acquired. Results: The major findings were as follows. In general, medium correlation effect sizes were found. Competency among sub-factors of BPN showed highest effects. Parental factor was more related to BPN than school and individual factors. Parenting behavior and family psychological backgrounds were the most crucial factors in the parental factors. School adjustment was the most critical factor in the school factors. In school grade, the elementary school was more related to BPN than junior high school and high school. Conclusion: Based on these results, we suggest a number of components for parent-education programs, and information for future research.

사례연구 : 반항성 장애 아동의 놀이치료 사례연구

채은영 ( Eun Young Chae ) 한국놀이치료학회 2005 한국놀이치료학회지(놀이치료연구) Vol.8 No.1

국내 집단 놀이치료 프로그램 효과에 대한 메타분석

채은영 ( Eun Young Chae ),정문주 ( Moon Joo Cheong ) 한국놀이치료학회 2016 한국놀이치료학회지(놀이치료연구) Vol.19 No.2

본 연구는 집단 놀이치료 효과에 대한 실증적인 자료를 마련하고 그에 따라 실제 임상 현장에서 집단 놀이치료를 실시할 때 도움이 될 수 있는 자료를 제공하고자 하였다. 이를 위하여 기존의 자료를 체계적으로 요약·정리 할 수 있는 메타분석의 방법을 사용하여 최근 10년간의 집단 놀이치료 프로그램의 효과에 대해 종합·분석하였다. 국내 학술지에 게재된 총 30편(효과크기 293개)의 논문을 분석대상으로 선정하여 메타 분석을 실시하였다. 집단 놀이치료 프로그램을 실시한 집단은 그렇지 않은 집단에 비해 전체 평균 효과크기에서 유의미한 효과를 나타냈다. 전체 집단 놀이치료 프로그램의 분류별 효과크기를 분석해 본 결과 게임놀이, 치료놀이, 놀이 활용, 표현예술놀이, 놀이치료 순으로 통계적으로 유의한 효과크기를 보였다. 집단 놀이치료 프로그램과 관련변인과의 효과 크기를 분석해 본 결과 아동 개인 변인, 양육자 변인, 관계 변인의 순이었다. 중재변인으로서 연구대상을 중·고, 영·유아, 초등학생으로 분류한 후 놀이치료프로그램의 효과성을 분석하는데, 효과크기는 중·고등학생, 영·유아, 초등학생의 순이었다. 총 회기 수에 따른 프로그램 효과크기는 10~20회기, 20~30회기, 10회기 미만의 순으로 통계적으로 유의하였고, 1회기 당 시간(분)을 범주화하여 각 효과크기와 차이검증을 실시한 결과, 30분 이상에서 40분미만의 효과크기가 가장 컸다. 마지막으로 출판연도를 연속 변인으로 코딩한 후 각 연도에 따른 효과크기를 회귀 분석하였는데 학술 논문에 있어서 최근 연도일수록 과거에 비해 놀이치료 프로그램의 효과가 상승한 것을 보여주었다. This study was aimed at gathering data, when engaged in group play therapy in the fields, and to also gather empirical data on the effects of group play therapy. For this purpose, we synthesized and analyzed the effects of group play therapy programs over the last ten years, using a meta-analysis-a systematic approach, by using summaries and reviews of the previous literature. We analyzed the studies published in major nations, 30 studies(effect size samples n= 293), were chosen, using meta-analysis. Group play therapy program groups showed much more significant effects than control groups. According to the results in terms of the effect size of the classification of the play therapy program types, it was statistically significant effective sizes in order of the game play, theraplay, using play, expressive-arts play, and play therapy. From the results of the analysis of the effect sizes in this group play therapy program and related variables, showed that there were statistically significant effective sizes in order of the infants individual variables, rearer variables, and related variables. The intervention variables were categorized according to whether the subjects were high school students, junior high school students, elementary students, or infants, and these intervention variables were then analyzed. The effect size order was as follows; high school students, junior high school students, infants, and elementary students. Statistical significance was observed in the order of 10~20 sessions, 20~30 sessions, under 10 sessions to effect sizes according to the total program sessions. Statistical significance was observed in order of whether there were 10~20 members, 20~30 members, under 10 members to effect sizes, according to the total subject members. Lastly, after coding the published years to integers, inputting variables and then doing the regression analysis according to the published years, there were also clear improvements observed on the effect sizes.

아동상담자란 직업을 통해 경험하는 것에 관한 질적 연구

채은영 ( Eun Young Chae ) 한국놀이치료학회 2009 한국놀이치료학회지(놀이치료연구) Vol.12 No.2

본 연구의 참여자는 아동상담자들이며 주된 관심은 `아동 상담자`란 직업을 가지고 살아가면서 참여자가 체험하는 것과 그것의 의미에 대한 것이다. 즉 아동상담자란 직업을 갖고 살면서 경험하는 것에 대해 있는 그대로 드러내고 그 경험의 의미에 대해 알아보고자 하는 것이 이 연구의 목적이다. 연구 참여자는 모두 파트타임으로 병원, 사설 상담센터 등에서 일하고 있는 경력 만 3-7년차의 아동상담자였다. 연구 방법으로는 현상학적 연구 절차 중에서 Giorgi가 제시한 방법을 사용하여 현상학적 기술을 하기 위해 노력하였다. 연구자는 아동상담자라는 직업을 가지고 살면서 경험하는 것에 대해 1) `내면의 문제의식에서 시작된 자연스러운 끌림`, 2) `변화에 대한 책임: 혼자 지기 대 함께 지기`, 3) `일상적 삶과 직업적 삶의 혼재: 아름다운 구속, 선입관과 제 머리 못 깎기`, 4) `기쁨, 보람, 그리고 소명의식` 등의 네 가지로 범주화하였다. 그리하여 아동상담자들은 직업을 통해서 삶의 의미를 찾고 경험하기 위해 지속적으로 노력하는 사람들이란 깨달음을 얻게 되었다. The participants of this study were counselors of children, and the main focus of the study is the participants` experiences as child counselors and the meaning of their experiences. In other words, the purpose of this study is to expose the various experiences of child counselors and the significance thereof. The participants are all part-time employees at hospitals or private counseling centers and have 3~7 years of experience as child counselors. Among the processes of Phenomenological studies, the method of study chosen for this study is the Phenomenological methodology that is suggested by Giorgi. The researcher organized the experiences of child counselors and the meaning of these experiences into the following four categories: 1) Natural affinity related to their inner problems, 2) Responsibility regarding changes: bearing it alone vs. bearing it together, 3) Balance between personal life and work life: beautiful bondage, preconceptions and not being able to scratch their own back, 4) Happiness, reward and a sense of calling. As a result, it showed that child counselors are people who find meanings in their lives and who continuously strive to gain various experiences.

허혈-재관류에 의한 신손상에 있어 Na+/H+ 교환체 1형 억제제의 신 보호작용

채은영 ( Eun Young Chae ),최범순 ( Bum Soon Choi ),양현주 ( Hyun Joo Yang ),안경옥 ( Kyung Ok Ahn ),양철우 ( Chul Woo Yang ),김용수 ( Yong Soo Kim ),방병기 ( Byung Kee Bang ) 대한신장학회 2006 Kidney Research and Clinical Practice Vol.25 No.6