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Pitch Measurement of 150 nm ID-grating Standards Using an Nanometrological Atomic Force Microscope
진종한,Ichiko Misumi,Satoshi Gonda,Tomizo Kurosawa 한국정밀공학회 2004 International Journal of Precision Engineering and Vol.5 No.3
Pitch measurements of 150 nm one-dimensional grating standards were carried out using a contact mode atomic force microscopy with a high resolution three-axis laser interferometer. This measurement technique was named as the nano-metrological AFM. In the nano-metrological AFM, three laser interferometers were aligned precisely to the end of an AFM tip. Laser sources of the three-axis laser interferometer in the nano-metrological AFM were calibrated with an I2-stabilized He-Ne laser at a wavelength of 633 nm. Therefore, the Abbe error was minimized and the result of the pitch measurement using the nano-metrological AFM could be used to directly measure the length standard. The uncertainty in the pitch measurement was estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM). The primary source of uncertainty in the pitch-measurements was derived from the repeatability of the pitch-measurements, and its value was about 0.186 nm. The average pitch value was 146.65 nm and the combined standard uncertainty was less than 0.262 nm. It is suggested that the metrological AFM is a useful tool for the nano-metrological standard calibration.
OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향
진종인,권태현,최병현,김성수,조현태,공일근 韓國受精卵移植學會 2010 한국동물생명공학회지 Vol.25 No.1
본 연구에서는 OPU를 통한 체내 유래 난자를 이용한 수정란 생산 시 1개월에서 6개월까지의 연구 기간에 따른 난포 생성수, 난자 회수율, 난자 등급율, 배반포 생성률을 분석하여 공란우의 활용 기간에 관하여 조사하였다. 1. 채란 기간에 따른 난포 생성수는 1개월에서 5개월까지의 난포 생성수는 , , , , 개로 유의적인 차이가 없었으나, 6개월째에는개로 급격하게 줄어든 것을 알 수 있었다. 2. 채란 기간에 따른 난자 회수개수는 1개월에서 3개월까지는 This study was performed to identify the optimal timing for oocyte donor replacement during OPU procedure. OPU was carried out to collect oocytes from every donor at an interval of days (2 times a week). The collected oocytes were matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml of FSH and 1 mg/ml of estradiol for 24 h. After 24 h of exposure to sperm, the presumptive zygotes were cultured in CR1aa medium supplemented with 4 mg/ml of BSA for 3 days before being changed to CR1aa medium with 10% of FBS for another days. The mean numbers of retrieved oocytes were remained constantly up to 3 months (, , ), but significantly decreased at over 4 to 6 months (, , ) (p<0.05). The blastocyst development potential was also very similar rate from 1 to 3 months (37.2%, 40.4% and 44.6%), but significantly decreased from 4 to 6 months (24.8%, 29.3% and 28.6%, respectively) (p<0.05). The production of OPU derived embryos in periods of 1 to 3 months (, and ) were significantly higher than those in 4 to 6 months (, and , respectively) (p<0.05). In conclusion, the efficient periods for the production of OPU derived embryos was until 4 months, twice per week to produce over 64 transferable embryos and then replace new donor after 3 months use. The best replacement time is 3 months and could be maximized production of OPU derived embryos.
진종인,최병현,김성수,조현태,선두원,임현태,이정규,민찬식,공일근 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.3
This study was carried out to establish the system of OPU derived embryo production, management of recipients as well as offspring production. OPU derived embryo production system was carried out of aspiration of immature oocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work was collected total 13,866 oocytes, average 8.2±4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8 oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavage and blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0±3.8 cleaved embryos and 3.0±2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 local governments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7% in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancy rate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in F at 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed 100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cow embryos as well as offspring by OPU derived embryo production system, of which could be decreased the required time of genetic improvement.
진종인,최병현,김성수,조현태,선두원,임현태,이정규,민찬식,공일근 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.3
This study was carried out to establish the system of OPU derived embryo production, management of recipientsas well as offspring production. OPU derived embryo production system was carried out of aspiration of immatureoocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system andthen produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work wascollected total 13,866 oocytes, average 8.2 ± 4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavageand blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0 ± 3.8 cleavedembryos and 3.0 ± 2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 localgovernments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7%in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancyrate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in Fat 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cowembryos as well as offspring by OPU derived embryo production system, of which could be decreased the requiredtime of genetic improvement.
간호대학생의 투지(Grit), 학업탄력성 및 심리적 안녕감
진종임(Jin, Jong Im),김남초(Kim, Nam Cho) 한국간호교육학회 2017 한국간호교육학회지 Vol.23 No.2
Purpose: This study was conducted to investigate the relationship among grit, academic resilience, and psychological well-being among nursing students. Methods: The participants were 271 nursing students, enrolled in C university. Data were collected from September 5 to 30, 2016 and analyzed using descriptive analysis, t-test, ANOVA, Pearson correlation coefficients, and stepwise multiple regression with the SPSS/WIN 21.0 program. Results: The mean scores for academic resilience were above average. The mean score for grit and psychological well-being presented an appropriate level. Psychological well-being in nursing students was significantly correlated with academic resilience (r=.65, p<.001) and grit (r=.52, p<.001). The significant predictors of psychological well-being for nursing students were academic resilience (β=.47, p<.001), grit (β=.26, p<.001), and major satisfaction (β=.20, p<.001) which explained 53.1% of the variance in psychological well-being. Conclusion: The results indicate that it is necessary to develop grit and academic resilience enhancement programs to improve the psychological well-being of nursing students.