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Immobilization of $\alpha$-Amylase and Glucoamylase on Porous Cellulose Beads
지영민,전문진,엠써네쓰,Chi, Y.M.,Chun, M.,Sernetz, M. 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.1
다공성 섬유소 업자를 제조하고, $\alpha$-amylase와 glucoamylase를 각각 효과적으로 고정화 시키기 위하여 섬유소 담체를 활성화 시키고 그 최적 조건을 조사하였으며 그것의 효소학적 성질에 관하여 연구하였다. 가장 효과적 인 $\alpha$-amylase와 glucoamylase의 고정화는 다공성 섬유소 담체를 0.1M p-benzoquinone으로 활성화 시킨 후 3% gallotannin으로 재활성화 시켰을 때 잔여 활성도가 가장 높았으며 이때 $CaCl_2$를 첨가함으로써 고정화율이 증가되었다. 유리의 $\alpha$-amylase와 고정화 $\alpha$-amylase의 반응 최적 pH는 차이가 없었으며, 반응 최적 온도는 모두 $55^{\circ}C$이었다. 유리의 glucoamylase와 고정화 glucoamylase의 반응 최적 pH에도 차이가 없었으나 반응 최적 온도는 유리의 glucoamylase는 $55^{\circ}C$, 고정화 glucoamylase는 $50^{\circ}C$로 나타났다. Starch에 대한 $\alpha$-amylase와 gucoamylase의 Km 값은 고정화시킴으로써 증가되었고 저장성 또한 증가되었다. For the immobilization of $\alpha$-amylase and glucoamylase effectively on porous cellulose beads, the optimal activation methods were studied. And their enzymatic properties were investigated. The results obtained were as follows; 1) The most effective method of enzyme immobilization was obtained when the 3% gallotannin, pH 4, was used as activation reagent after 0.1M p-benzoquinone. pH 12, activation in case of $\alpha$-amylase and when the 3% gallotannin, pH 9, was used as activation reagent after 0.1M p-benzoquinone, pH 12, activation in case of glucoamylase. 2) The immobilized enzymes activities were increased by the addition of $CaCl_2$ to immobilizing mixture. The immobilized $\alpha$-amylase activity was increased from 69.17u/g to 77.18u/g by addition of $5{\times}10^{-4}M$ $CaCl_2$ to the immobilizing mixture. The immobilized glucoamylase activity was increased from 157.37u/g to 189.31u/g by addition of $5{\times}10^{-3}M$ $CaCl_2$ to the immobilizing mixture. The maximum immobilization rate of $\alpha$-amylase was 5.07% and glucoamylase was 47.33%. 3) The optimum pH of $\alpha$-amylase was 4.5-5 for native enzyme and also 4. 5-5 for immobilized enzyme. The optimum pH of glucoamylase was 4.5 in both cases. 4) The optimum temperatures for the native and immobilized $\alpha$-amylase were both $55^{\circ}C$. The optimum temperature was $55^{\circ}C$ for the native glucoamylase and $50^{\circ}C$ for the immobilized glucoamylase. 5) The Km value of native $\alpha$-amylase was 0.48% and that of immobilized $\alpha$-amylase was 1.43% while Vmax (1.52mg hydrolyzed soluble starch/min) was unaltered. The Km value of native glucoamylase was 0.83% and that of immobilized glucoamylase was 2.0%.
지영민,옥기수,유준재 한국정보통신설비학회 2018 한국정보통신설비학회 학술대회 Vol.2018 No.08
Currently, Korea government is pushing to reduce the energy of various building to meet GHG(Greenhouse Gas) reduction target. There are various methods to save energy in order to fulfill this reduction obligation. One of the ways is detection of spaces using IoT environment sensors to save energy on buildings based on monitoring. Some spaces waste the energy to meet thermal comfort level even when there is no people. In this paper, we show the practical results of our occupancy detection algorithm using IoT environment sensors to find the wasted space on the building.
대용량 분산 클러스터 기반의 시계열 빅-데이터 처리 및 수집 시스템
지영민,유준재,서희 한국정보통신설비학회 2016 한국정보통신설비학회 학술대회 Vol.2016 No.09
The technology of IoT which began in the sensor networks generate numerous sensing data from a variety of physical environments to virtual world. There are many types of IoT device used the sensors based on application. They usually create time series data that people can use to make more sensible decision. Things produced various data which is generated and stored as time based and then saved them to the existing database. In this paper, we introduce the system architecture for the large distributed cluster based system for the processing and collecting of big-data of time-series to meet the goal of performance using Redis Cluster and OpenTSDB.
지영민,옥기수,유준재 한국정보통신설비학회 2018 한국정보통신설비학회 학술대회 Vol.2018 No.08
The 4th industrial revolution in recent years is a term used to describe a new paradigm based on IoT (Internet of Things), Big-data, and Artificial Intelligent technology. IoT means collecting data from the real world and sending them to the internet and accumulating as the big data. This accumulated data aims at making intelligent system by using artificial intelligence as learning data. In this paper, we show how it is possible to control actual facilities using artificial intelligence reinforcement learning by using facility data accumulated through IoT and big data.
다공성 섬유소 입자에 대한 α - Amylase 와 Glucoamylase 의 고정화
지영민,전문진,엠써네쓰 ( Y . M . Chi,M . Chun,M . Sernetz ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1
For the immobilization of α-amylase and glucoamylase effectively on porous cellulose beads, the optimal activation methods were studied. And their enzymatic properties were investigated. The results obtained were as follows; 1) The most effective method of enzyme immobilization was obtained when the 3% gallotannin, pH 4, was used as activation reagent after 0.1M p-benzoquinone, pH 12, activation in case of α-amylase and when the 3% gallotannin, pH 9, was used as activation reagent after 0.1M p-benzoquinone, pH 12, activation in case of glucoamylase. 2) The immobilized enzymes activities were increased by the addition of CaCl₂ to immobilizing mixture. The immobilized a-amylase activity was increased from 69.17 μ/g to 77.18μ/g by addition of 5×10^(-4)M CaCl₂ to the immobilizing mixture. The immobilized glucoamylase activity was increased from 157.37μ/g to 189. 31μ/g by addition of 5×10^(-3)M CaCl₂ to the immobilizing mixture. The maximum immobilization rate of a-amylase was 5.07 and glucoamylase was 47.33%. 3) The optimum pH of α-amylase was 4.5-5 for native enzyme and also 4.5-5 for immobilized enzyme. The optimum pH of glucoamylase was 4.5 in both cases. 4) The optimum temperatures for the native and immobilized α-amylase were both 55℃. The optimum temperature was 55℃ for the native glucoamylase and 50℃ for the immobilized glucoamylase. 5) The Km value of native a-amylase was 0.48% and that of immobilized α-amylase was 1.43% while Vmax (1.52㎎ hydrolyzed soluble starch/min) was unaltered. The Km value of native glucoamylase was 0.83% and that of immobilized glucoamylase was 2.0%.
하악골에 발생한 Gorham-Stout 증후군: 증례 보고
지영민,송진아,신정현,방강미,김성민,명훈,서병무,최진영,이종호,정필훈,김명진,황순정,Ji, Young-Min,Song, Gin-Ah,Shin, Jung-Hyun,Pang, Kang-Mi,Kim, Soung-Min,Myoung, Hoon,Seo, Byoung-Moo,Choi, Jin-Young,Lee, Jong-Ho,Choung, Pill-Hoon,Kim, 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.6
Gorham-Stout 증후군은 조직학적으로 양성의 혈관 증식을 보이면서 광범위한 골 용해 현상을 나타내는 희귀한 병으로 알려져 있다. 병인론은 아직 명확히 밝혀져 있지 않으며 주로 남성과 젊은 성인에게서 호발하는 것으로 알려져 있다. 진단에는 컴퓨터 단층촬영, 자기공명영상, 핵의학검사, 혈관조영검사 등의 영상검사가 도움이 되며, 조직학적검사 역시 몇몇 특이 소견을 통해 진단에 도움을 줄 수 있다. 발생하는 부위에 따라서 그 예후는 매우 다르며, 특히 척추나 흉곽에 발생할 경우는 생명을 위협할 수 있다. 저자등은 우측 하악골의 병적 골절을 주소로 내원한 환자에서 임상검사, 방사선학적 검사, 조직병리학적 검사를 통해 Gorham-Stout 증후군으로 진단한 환자를 비골유리피판을 이용한 하악골 재건술을 통해 치료하였고 4년 동안 관찰 기간 중 재발 없이 양호한 치유 경과를 보였기에 문헌고찰과 함께 보고하는 바이다. Gorham-Stout disease is a rare skeletal disease which is progressive and spontaneous and idiopathic resorption of whole body bone. In the initial stage, the osteolytic site is replaced by histologically benign vascular proliferation without new bone formation and finally by dense fibrous tissues. We encountered with a patient involving the mandible with attention by the clinical, radiographic, scintigraphic, angiographic, and computed tomography. We resected the mandibular lesion which was reconstructed with a fibular free flap. We report literature review with a mandibular lesion involved with Gorham-Stout disease.