http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이지은 ( Ji Eun Lee ),이선경 ( Seon Kyung Lee ),지성길 ( Sung Gil Chi ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12
목적 : 침윤성 자궁 경부암 조직에서 신생혈관생성 및 VEGF, TSP-1의 발현 정도를 조사하여 자궁 경부암의 진행 과정에 있어 이들의 상호 관계 및 예후인자로서의 가치를 평가하고자 하였다. 연구 방법 : 자궁경부암 37예와 7예의 정상 자궁경부 조직 절편을 채취하여 VEGF 아단위와 TSP-1의 mRNA 정량분석을 하였다. 미세혈관수는 면역조직화학 염색을 통해 검사하였다. 결과 : 정상 조직과 자궁경부암 조직 모두에서 VEGF와 TSP-1이 발현되었으며, 정상조직에서 발현된 VEGF와 TSP-1의 mRNA값의 평균값보다 2배 이상 발현된 경우를 과발현 (over expression), 1/2 이하의 값을 가지는 경우는 저발현 (low expression)으로 정의하였다. VEGF121은 56.8%에서, VEGF165는 40.5%에서 과발현을, TSP-1은 35.1%에서 저발현을 보였으며 정상 조직에서는 과발현이나 저발현이 나타나지 않았다. 미세혈관수는 VEGF의 과발현과 유의한 상관관계를 보였으나 (p<0.05), TSP-1의 저발현과는 상관관계가 없었다. VEGF의 과발현과 TSP-1의 저발현이 동반되어 나타난 그룹에서의 미세혈관수도 의미 있게 증가하였다 (p<0.05). 결론 : VEGF와 TSP-1의 발현에 따라 미세혈관의 수가 의미 있게 변화하는 것으로 보아 침윤성 자궁경부암에서 VEGF 과다 발현과 TSP-1의 저발현이 함께 나타나는 경우 미세혈관 형성을 촉진시키는 것으로 사료된다. Objective: Vascular Endothelial Growth Factor (VEGF) is a potent stimulator of angiogenesis in solid tumors. Thrombospondin-1 (TSP-1) has inhibitory role in cancer cell proliferation and metastasis. To analyze the correlation with expression of VEGF and TSP-1 including microvessel density (MVD), the levels of VEGF/TSP-1 mRNA expression and microvessel count (MVC) were estimated in patients with invasive cervical carcinomas. Methods: From 1996 to 1999, 37 carcinomas and 7 normal cervical tissues were collected, frozen and stored at -70℃ until used. The levels of VEGF and TSP-1 mRNAs were determined by quantitative RT-PCR. MVD was assessed by immunostaining for factor VIII-related antigen. The results are expressed as the largest number of microvessels present within a single ×40 field, and counted at x 100 field. Results: Quantitative RT-PCR analysis demonstrated abnormally increased VEGF mRNA expression levels (>0.66) in 14 (37.8%) of 37 cervical carcinomas comparing to control groups (mean: 0.32±0.09) and abnormally low TSP-1 mRNA expression levels (<0.72) in 13 (35.1%) of 37 cervical carcinomas comparing to control groups (mean: 0.51±0.07). MVC was higher in tumors showing decreased expression of TSP-1 (but not statistically) (p<0.18) and overexpression of VEGF (p<0.05). When VEGF overexpression was accompanied with reduced TSP-1 expression, the microvessel density showed significantly increased pattern (p<0.05). Conclusion: Our study demonstrates that reduced expression of TSP-1 mRNAs and overexpression of VEGF mRNAs may be an important contributing factor in cervical carcinomas. Moreover, the inversed correlation of VEGF and TSP-1 mRNA expression can be an evidence of angiogenic role in cervical carcinomas.
수종 한약제의 위암세포에 대한 항암작용 효능 검색 및 약리작용에 관한 분자생물학적 연구
김진성,이지향,류봉하,박재훈,지성길,유진화,Kim, Jin-Seong,Lee, Ji-Whyang,Ryu, Bong-Ha,Park, Jae-Hoon,Ji, Sung-Gil,Yoo, Jin-Hwa 대한암한의학회 1999 大韓癌韓醫學會誌 Vol.5 No.1
Traditional oriental medicines have been used for treatment of various kinds of human cancers for long times and some of them proven to be effective clinically. However, the pharmacological actions and mechanisms related to cancer treatment are generally unknown. In an effort to clarify the action mechanisms of several oriental medicines used for cancer treatments, we planned this experimental procedures. We selected Cordyceps sinensis (冬蟲下草), Punellae Herba (夏枯草), Rehmanniae Radix (熟地黃), Paeoniae Radix (白芍藥), Oldenlandiae Herba (白花蛇舌草), Partulaceae Herba (馬齒? ), Scdopendra subspinipes mutilans (蜈蚣), Mylabris Phalerara (班蟄), Phellinus igniarius(桑黃), Ganodermae Lignum(靈芝) for evaluation, which have been used for patients of gastric cancers. The twenty grams of medicines were boiled in 100ml of water for 1 hour and filtered with $0.2\;{\mu}m$ pore-sized filter unit to remove insoluble particles. Initially we evaluated the effects oriental medicines on growth inhibition in stomach cancer cells. The gastric cancer cell line, AGS, was cultured in RPMI 1640 supplemented with l0% heat-inactivated fetal bovine serum and treated with $10{\mu}l$ oriental medicines per 1ml of medium up to 48 hours. The specimens were subjected to MTT assay for evaluation of growth inhibition. We found mat Mylabris phalerata (班蟄) markedly suppressed the growth of cancer cells as shown in results. Next, we checked the effects of oriental medicines on cancer on cell cycles and apptosis. Mylabrls phalerata (班蟄) inhibited cell cycle progression of cancer cells a compared with control cells and cells treated with other medicines. In addition, Mylabri phalerata (班蟄) induced apoptosis in 30-40% of stomch cancer cells within 24 hours. Other oriental medicines used for this experiments did not show apoptosis-inducing effects on cancer cells. Finally, we determined the expression levels of genes associated with cell cycle and apoptosis. The expressions of Bcl-2 and bcl-XL were downregulated by the treatment of Mylabris phalerata (班蟄). However, the expression levels of genes related to cell cycles were not altered significantly. In conclusion, we found that Mylabris phalerata (班蟄) has in vivo gowth-inhibiting and apptosis-inducing effects on stomach cancer cells. However, we think that at least animal experiments are necessay for evduations.
대뇌 핍지교종이 동반된 Turcot 증후군 1예의 유전학적 분석
김한수 ( Han Soo Kim ),박지영 ( Ji Young Park ),김효종 ( Hyo Jong Kim ),지성길 ( Sung Gil Chi ),김윤화 ( Yoon Hwa Kim ),이길연 ( Kil Yeon Lee ),정용희 ( Yong Hee Joung ),한요셉 ( Yo Seb Han ),동석호 ( Seok Ho Dong ),김병호 ( Byung 대한장연구학회 2003 Intestinal Research Vol.1 No.2
Turcot`s syndrome (TS) is a genetic disease characterized by primary brain tumor, colon cancer and/or multiple colorectal polyps. The mode of genetic transmission of the syndrome still remains unclear because TS is a rare disorder. The majority of central nervous system (CNS) neoplasms associated with TS are glioma, glioblstoma multiformes and medulloblastoma. Other types of CNS tumors related to TS have been noted in a few case reports, and there are only two reports of oligodendroglioma associated with TS. To the authors` knowledge, this is the first case of a patient with TS who had a cerebral oligodendroglioma and a colorectal adenocarcinoma in Korea. Therefore, the authors performed genetic analysis of this patient and her family to determine the genetic variants, including mutations in APC gene and mismatch repair gene, in Turcot`s syndrome. (Intestinal Research 2003;2:192-196)
고충전 인쇄용지 제조를 위한 중질 탄산칼슘 전처리 기술의 안정성에 관한 연구
서영범 ( Yung Bum Seo ),최진성 ( Jin Sung Choi ),지성길 ( Sung Gil Ji ) 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.1
인쇄용지에 탄산칼슘을 많이 넣을수록, 즉 고충전 인쇄용지를 만들수록 펄프섬유의 사용량이 줄어들고, 건조비용이 감소함으로 생산비는 절감되며, 온실가스의 배출량도 적어지게 된다. 현재까지 고충전 인쇄용지는 주로 중질탄산칼슘(GCC. ground calcium carbonate)에 기능성고분자를 첨가하여 적절한 크기로 선응집(pre-flocculation)시켜 사용함으로서 기존의 인쇄용지 제조방법에 비해 고충전시에도 인쇄용지의 중요한 특성들인 인장강도의 저하를 줄이고, 평활도를 유지시켜왔다. 하지만 GCC의 선응집체는 만들어진 후 사용하기까지 시간이 지체되면 그 크기와 성질이 변하는 불안정성을 보였다. 본 연구에서는 GCC의 선응집기술을 개량하여 선응집된 GCC사이에 탄산칼슘을 화학적으로 새로 생성시켜 GCC간에 연결을 시도하였으며, 그 결과 안정성이 높은 선응집체가 형성되었고, 이를 HCC (hybrid calcium carbonate)로 명명하였다. HCC는 GCC 선응집체와 같이 종이의 강도를 높이고, 평활도를 유지시켰으며, GCC 선응집체의 단점인 벌크의 저하를 역전시켜 높은 벌크를 형성시키는 장점을 보였다. More addition of calcium carbonate in printing paper allows savings of the wood fibers and the drying energy. Pre-flocculation of GCC (ground calcium carbonate) using functional polymers was known as the best available technology to make high loaded paper until now, and it allowed less reduction of the paper essential properties such as tensile strength and smoothness at higher GCC content. However, pre-flocculated GCC became unstable in size under the continued agitation in the mill. Therefore, pre-flocculation method was modified in such a way that the in-situ cal-cium carbonate was formed between the GCC particles of the pre-flocculated GCC, and the resultant became more sta-ble in size, which we named as HCC (hybrid calcium carbonate). HCC turned out to make high tensile strength and smoothness as much as the pre-flocculated GCC and gave much better size stability against stirring. Furthermore, HCC gave high bulk that pre-flocculation could not make.
목분과 전분을 이용한 골판지원지의 건조효율 및 물성향상
서영범(Yung Bum Seo),정재권(Jae Gwon Jung),지성길(Sung Gil Ji) 한국펄프·종이공학회 2016 펄프.종이技術 Vol.48 No.2
The increase of wet web solid content after wet press and dry compressive strength were observed in lab study by judicious application of wood flour and starch for the old corrugated container (OCC). Pearl starch was better than cationic starch in strength development, but cationic starch was better for drainage. Application of vacuum on the mixed solution of wood flour and starch helped strength development further without loss of other properties. The effect of wood flour addition on wet web solid content improved as the wet pressing pressure increased. The use of wood flour and starch mixture improved wet web solid contents further.