식물성장 조절물질을 분비하는 미생물의 탐색

조봉희,김근,성낙문 ( Bong Heuy Cho,Keun Kim,Nack Moon Sung ) 한국균학회 1993 韓國菌學會誌 Vol.21 No.2

Streptanthus tortus 배양세포에서 미생물 Elicit가 사부형성에 미치는 영향

조봉희,Cho, Bong-Heuy 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Extracts of Escherichia coli as a elicit were treated to suspension cultures of Streptanthus tostus in order to observe the effect on the pholem development. By the elicit treatment, cell wall, sieve endoplasmic reticulum (SER) and p-protein were normally synthesized, but the structure of amyloplast was changed from a round form to irregular and swollen unhalthy form with a tiny starch granular. Oil drops were new synthesized and accumulated in a large oleoplast and proteins were also accumulated in a single membrane. The concentration of sucrose in the phloem, which was induced during the elicit treatment, was higher than normally developed phloem cells. These results suggest that phloem cells might be changed in the normal cycles of metabolism of lipids, carbohydrates and proteins to overcome during the eilicit stress.

무의 자엽과 하배축에서 ABA 처리동안 Polyamine 농도 변화의 분석

조봉희,박선영,Cho, Bong-Heuy,Park, Sun Young 한국분석과학회 2001 분석과학 Vol.14 No.5

자엽에 ABA를 처리하면 세포내에 있는 유리 PA은 1시간 이내에 $500{\mu}mole$에서 $290{\mu}mole$로, SPD는 $153{\mu}mole$에서 $79{\mu}mole$로, SPM은 $69{\mu}mole$에서 $20{\mu}mole$로 점진적으로 감소되었다. 2일동안 지속적으로 ABA를 처리하면 PUT는 $290{\mu}mole{\sim}234{\mu}mole$ 사이의 일정한 수준을 유지하면서 증감의 변화만을 보였다. 하배축에다 ABA를 처리하면 자엽에서와 같이 유리 PUT는 1시간 이내에 $160{\mu}mole$에서 $9{\mu}mole$까지 점진적으로 감소되었다. 2일동안 지속적인 ABA 처리동안 하배축에 있는 유리 PUT과 다른 PA들은 $5{\mu}mole$ 정도로 존재하였다. ABA처리는 생체내에 존재하는 유리 PA의 농도를 감소시켜 직접적으로 스트레스에 관여하는 것이 아니라 스트레스에 대항하여 생체가 미리 준비하도록 생리 및 생화학적인 변화를 조절하는 것으로 보인다. The concentration of free PAs in the cells was decreased gradually from $500{\mu}mole$ to $290{\mu}mole$ for PUT, from $153{\mu}mole$ to $79{\mu}mole$ for SPD, from $69{\mu}mole$ to $20{\mu}mole$ for SPM during the ABA treatment within 1 hour, and was slightly changed with the increasing or decreasing between $290{\mu}mole{\sim}220{\mu}mole$ in the constant content level of PUT during the continuously ABA treatment of 2 days in the young cotyledons. The concentration of free PUT was gradually decreased from $160{\mu}mole$ to $9{\mu}mole$ during the ABA treatment within 1 hour like as cotyledons, and decreased from $9{\mu}mole$ to $5{\mu}mole$ during the continuously ABA treatment 2 days in the young hypocotyls. PUT and other PA were existed $5{\mu}mole$ during the continuously ABA treatment of 2 days in the hypocotyls. It showed that during the ABA treatment was decreased all PA concentration in the cells and PAs were not concerned directly to stress, but might regulated physiological change against stress.

Streptanthus tortus 조직배양 세포에서 사공의 형성시기와 사공 영역과 사공의 크기 결정

조봉희,Cho, Bong-Heuy 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1

Streptanthus 조직배양 세포에서 유조직 세포로부터 사부 세포의 발달 동안 설탕 운반자는 유도되었고, 단당류인 포도당의 운반자는 사라졌다 (Cho 1998). Streptanthus 조직배양 세포에서 유도된 사부영역과 사공은 sieve endoplasmic reticulum (SER)과 p-protein 합성이 거의 완성되는 시기에 형성되며, SER이 새로 합성된 세포벽을 에워싼 후에 세포벽이 분해되기 시작하였다. 세포벽의 분해와 사공의 형성은 비교적 규칙적으로 진행되었다. 사공의 모양은 계란형이었고, 사공의 외부크기는 세로 1.2 $\mu\textrm{m}$~l.6 $\mu\textrm{m}$, 가로 0.8 $\mu\textrm{m}$~l.3 $\mu\textrm{m}$, 다양하고 사공의 내부크기는 별모양과 유사한 불규칙한 형태였다. 두 사부세포 사이에서 사공의 수는 $\mu\textrm{m}$$^2$당 2개~7개였고, 육상체를 포함한 사공벽에 두께는 0.05 $\mu\textrm{m}$~0.07 $\mu\textrm{m}$,의 두꺼운 벽으로 구성되었다. 사공영역과 사공의 형성에 필요한 에너지는 세포벽 가까이에 위치하고 있는 미토콘드리아에서 얻을 것으로 추측하며, 사공 형성에 대한 SER의 역할은 아직까지 설명할 수 없었다. During the phloem development from parenchyma cells in a suspension culture of Streptanthus induced sucrose carrier and glucose carrier disappeared. Sieve element area and sieve pore induced suspension culture of Streptanthus were formed almost at the last period of the synthesis of sieve endoplasmic reticulum (SER) and p-protein. The new synthesized cell wall begann to digeste only after the new cell wall was surrounded by SER. The digested region of the cell wall and the formed region of sieve pore were regular comparatively. The completed sieve pore was an oval form, and the outer portion of sieve pore varied, ca 1.2 ${\mu}{\textrm}{m}$~1.6 ${\mu}{\textrm}{m}$ in longitudinal, 0.8 ${\mu}{\textrm}{m}$~1.3 ${\mu}{\textrm}{m}$ in tangential, and the inner size of sieve pore was irregular form of a star-like shape. The number of sieve pore between sieve cells was ca 2~7 per ${\mu}{\textrm}{m}$$^2$ and the sieve pore wall with callose was 0.05 ${\mu}{\textrm}{m}$~0.07 ${\mu}{\textrm}{m}$ in thickness. The energy for the formation of sieve element area and sieve pore might be supplied by mitochondria near the new cell wall and the role of SER remains to be illucidated.

Streptanthus tortus 자엽의 배양세포에서 사부세포 발달동안 Sucrose 능동수송계의 유도 시기

조봉희,Cho, Bong-Heuy 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.2

Parenchyma cells of Streptanthus tortus suspension cultures possessed the different transport system for aldose-formed D-glucose and for ketose-formed D-fructose. $K_{m}$ value for D-glucose and D-fructose were 0.28mM and 15.02mM, respectively. $K_{m}$ value of D-mannose was 0.44 mM which is similar to the D-glucose transport system, but D-mannose was transported also through its own special uptake system. Parenchyma cells possessed the transport system of L-glucose, but the function of L-glucose was not known at all. Protoplast of parenchyma cells possessed only the monosugars transport system, but didn't possess the disugars, sucrose transport system. Early developing phloem protoplasts possessed glucose and sucrose transport system at the same time. On the contrary, in the complete developed phloem cells disappeared preexisted glucose transport system in the parenchyma cells, only new induced sucrose transport system existed.ted. 유소직 세포들은 aldose인 D-glucose ketose인 D-fructose에 대하여 다른 능동 수송계를 소유하고 있었다. D-glucose와 D-fructose 수송계의 $K_{m}$ 값은 각각 0.28 mM과 15.02mM이었다. D-mannose는 $K_{m}$ 값이 0.44 mM로 D-glucose와 유사하였지만, 그러나 D-glucose와는 다른 수송계를 소유하고 있었다. L-glucose도 고유한 수송계를 통하여 세포내로 수송되었으며, 그러나 그 기능을 전혀 알지 못하고 있다. 유조직 원형질체는 단당류 능동 수송계만을 소유하고, 이당류인 sucrose능동 수송계는 소유하지 않고 있었다. 발달 초기단계에 있는 사부 원형질체는 glucose와 sucrose 수송계를 동시에 소유하고 있었다. 완전히 발달된 사부세포에서는 이미 존재하였던 glucose 능동 수송계는 사라지고, 새로 유도된 sucrose 능동 수송계만 존재하는 것으로 측정되었다.

원적외선 방사물질 제조 및 물질의 특성 분석

조봉희(Bong-Heuy Cho) 한국분석과학회 2008 분석과학 Vol.21 No.4

[화학] 전기영동에 의한 피마자 자엽과 뿌리에서 Abscisic acid(ABA) 처리에 의한 단백질의 분석

조봉희(Bong- Heuy Cho),박선영(Sun Young Park),이종호(Jong Ho Lee) 한국분석과학회 1996 분석과학 Vol.9 No.1

Chlorella vulgaris 의 당류 능동수송계에 미치는 칼슘 이온의 영향

조봉희 (Bong Heuy Cho) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.4

Sugar uptake is accompanied with H^+ -substrate-symport generally. Both H^+ /sugar-and H^+/K^+ stoichiometries during the sugar-uptake have been reported to be exactly 1 : 1. This paper reports that the stoichiometries were enhanced dramatically by the addition of CaCl_2 into the medium and by the high cell density of 200 ㎕ pc/mL. The concentration of free Ca^2+ ions in the cells increased significantly with cell densiy. It is suggested that the free Ca^2+ ions are responsible for the change of stoichiometry of sugar transport system by regulation of H^+ ion level of biomembrane.

[화학] UV-분광광도법과 전기영동에 의한 피마자 유식물에서 저온처리에 의한 당류 함량과 단백질 구성의 분석

조봉희(Bong-Heuy Cho),박선영(Sun Young Park),박면용(Myon-Yong Park) 한국분석과학회 1995 분석과학 Vol.8 No.2

조직 배양에서의 과당의 능동 수송에 대한 Low Affinity System 의 분석

조봉희 (Bong Heuy Cho) 한국식물학회 1987 Journal of Plant Biology Vol.30 No.4

Undifferentiated suspension culture cells had the ability to transfer glucose and fructose actively, but the suspension culture cells were unable to transfer saccharide without previously splitting to monosaccharides. The uptake of fructose showed the low- and high-affinity system compared to of glucose, which possessed only one saturable uptake system. In this paper, the low affinity system of the uptake of fructose has been studied intensively. Glucose did not inhibit the low affinity system of fructose competitively. The Km value was 47 mM for fructose, 7.4 mM for glucose and Vmax was 69 μmol/h.g fresh weight for fructose, 9.8 μmol/h.g fresh weight for glucose. Metabolizer inhibitors, both 50 μM of CCCP and DNP, inhibited 70% of the uptake of the low affinity system of fructose. The proton ions were accompanied by the uptake of fructose. The stoichiometry showed ratio of proton to fructose was 0.17. The mechanism of the uptake was fructose-proton-symport. The molecules of fructose accumulated inside 25 times more than outsde. Therefore, the low affinity system of fructose was not mere diffusion, but depended on metabolic energy and thus transported actively. The importance of this system was discussed.