http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Helicobacter pylori Strain 51의 보유한 Type II 제한효소 (Hpy51-I)의 특성
조명제,박정욱,전병삼,박정원,변은영,이선경,박예형,송재영,이우곤,백승철,최여정,정선애,최미영,Cho, Myung-Je,Park, Jeong-Uck,Jeon, Beong-Sam,Pack, Jeong-Won,Byun, Eun-Young,Lee, Sun-Kyung,Park, Ye-Hyoung,Song, Jae-Young,Lee, Woo-Kon,Baik, Seun 대한미생물학회 2001 Journal of Bacteriology and Virology Vol.31 No.3
This study describes the purification and characterization of type II restriction endonuclease of Helicobacter pylori in order to understand the DNA restriction and modification of H pylori. H pylori cell extract was subjected to polyethyleneimine treatment, salt precipitation, heparine-sepharose column chromatography, and fast protein liquid chromatogrphy (FPLC) using Resource Q column and Mono Q column to purify the type II restriction endonuclease. Hpy51-I was characterized to recognize the sequneces 5'-GT(G/C)AC-3', yielding 5-base 5' protruding ends. The restriction sequence was identical to that of Tsp 45 I. The enzyme exhibited its maximal activity in the presence of $10{\sim}20\;mM$ NaCl, but was inhibited completely in the presence of more than 80 mM NaCl. The enzyme showed its maximal activity in the presence of $1{\sim}10\;mM\;MgCl_2$. The optimal pH and temperature for enzyme activity was pH 9.0 and $37^{\circ}C$, respectively. $MnCl_2$ could not substitute for $MgCl_2$ in reaction mixture. And addition of ${\beta}$-mercaptoethanol and bovine serum albumin in reaction mixture led to loss of enzyme activity of Hpy51-I. The whole cell extract of H. pylori strain 51 was confirmed to carry the enzyme activity for methylation of Hpy51-I-recognised sequence. Hpy51-I digested genomic DNAs of enteric bacteria to less than I kb while it could not cut the genomic DNAs of H. pylori isolates. In this study, the type II restriction enzyme (Hpy51-I) of H. pylori was identified and characterized its biochemical properties, demonstrating that Hpy51-I might be one of the barriers for preventing the introduction of foreign DNAs into H. pylori.
Helicobacter pylori Strain 51이 보유한 Type Ⅱ
조명제(Myung-Je Cho),박정욱(Jeong-Uck Park),전병삼(Beong-Sam Jeon),박정원(Jeong-Won Pack),변은영(Eun-Young Byun),이선경(Sun-Kyung Lee),박예형(Ye-Hyoung Park),송재영(Jae-Young Song),이우곤(Woo-Kon Lee),백승철(Seung-Chul Baik),최여정(Y 대한미생물학회 2001 Journal of Bacteriology and Virology Vol.31 No.3
김태우,김효은,조명제,강영화,이재동,Kim, Tae-woo,Kim, Hyo-eun,Cho, Myung-je,Kang, Young-hwa,Lee, Jae-dong 대한침구의학회 2003 대한침구의학회지 Vol.20 No.1
Objective : Hemifacial spasm is painless uncommon disorder characterized by involuntary paroxysmal movement of one side of face, In this study, the effect of Dong-Si acupuncture therapy for three patients with hemifacial spasm were evaluated. Methods : We have treated them with acupuncture treatment of Dong-Si acupuncture points(Cheuk-Samni and Cheuk-ha-Samni) and evaluated the effect by Scott's scale, For the first two weeks, it was dine once a day, and after two weeks, the other day. Results : Before therapy, the grades of spasm intensity classified by Scott's description in three cases were 3. And after Dong-Si acupuncture therapy, the grade of one case was 0, and two cases were 1. Conclusion : The Dong-Si acupuncture therapy for hemifacial spasm was effective and will be attempted to the patients with it.
Mycobacterium tuberculosis 파쇄 추출항원에 대한 단세포군 항체의 생산 및 그 특성에 관한 연구
국윤호 ( Yoon Hoh Kook ),조명제 ( Myung Je Cho ),황응수 ( Eung Soo Hwang ),김익상 ( Ik Sang Kim ),차창룡 ( Chang Yong Cha ),장우현 ( Woo Hyun Chang ),이승훈 ( Seung Hoon Lee ),김상재 ( Sang Jae Kim ),한용철 ( Yong Chol Han ) 대한결핵 및 호흡기학회 1984 Tuberculosis and Respiratory Diseases Vol.31 No.3