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白鼠 急性虛血性 骨격筋의 組織學的 및 組織化學的 變化에 미치는 Dimethyl Sulfoxide의 影響
이동근,손창신,양경무,조남표,정신호,최호열 의과학연구소 1991 全北醫大論文集 Vol.15 No.3
This experimental study was carried out to investigate the effect of dimethyl sulfoxide on the experimentally induced ischemic injury of skeletal muscle. The ischemic injury of the skeletal muscle was induced by the atraumatic clamping of the common iliac artery, and then reperfused after 1 hour and 5 hours, respectively. Immediately after reperfusion, experimental group were received 5㎖ of 20% solution of DMSO intravenously with a constant rate(0.5㎖/min) using infusion pump,and control group were received an equal volume of physiologic saline. histological and enzyme histochemical stains for H&E, trichrome, PAS, SDH and reversed myosin-ATPase on the flash-frozen sections of soleus muscle were performed on the 24hours after operation. The following results were obtained. 1.The soleus muscle ischemic for 1 Hour revealed only focal edema of the endomysium and perimysium. And the muscle ischemic for 5 hours exhibited pyknosis and focal disappearance of the sarcolemmal nuclei, flocculation of the sarcoplasm, endomysial and perimysial edema, and discoidal degeneration of muscle fibers. But there was no difference between the control and DMSO-treated group. 2.The total number of muscle fibers per unit area in the group of 5 hours ischemia was decreased in comparison with the normal rats. But the difference between the control and DMSO-treated group was not significant. 3.The proportion of muscle fiber types in both ischemic groups equally showed decrease in S-type onPAS stain. But there was no difference between the control and DMSO-treated group. These experimental results demonstrated that dimethyl sulfoxide revealed no protective effect against the acute ischemic injury of the skeletal muscle.
Ehanol에 依하여 損傷된 胃粘膜 上皮의 回復에 關한 實驗的 硏究
최삼임,이혜수,고범권,장성강,강명재,조남표,조성남,김상호 의과학연구소 1992 全北醫大論文集 Vol.16 No.2
This study aimed to investigate muoosal damage and restitution in the rat stomach after absolute ethanol instillation. Four me. of absolute ethanol was instilled to stomach and gastric mucosa was exposed to it for 30 and 45second , and then examined at immediately, 10 minute, 30 minutes, 60 minutes and 6 hours. Morphological studies of gastric tissue were performed by means of epon-toluidine blue section, H&E, and periodic acid Schiff(pAS) stains. The results were as follows. 1. Immediately after exposure to absolute ethanol far 30· seconds(time 0), surface mucous cells and the cells lining the gastric pits were damaged and exfoliated, but the gastric gland cells were not damaged. 2. Immediately after exposure to absolute ethanol for 45 seoends(time 0), superficial epithelium was necrotic and cellular damage was evident in the gastric glands beneath the damaged surface and gastric pits. Some inflammatory reaction was also noted. 3. Hyperemia and hemorrhage in the gastric mucosa were evident in almost all experimental groups 4. Epithelial restitution was begun to observe at 30 minutes in both 30 and 45 seconds-exposure groups, and almost completely restituted at 60 minutes after ethanol exposure. Surface mucous cells in restituted area were somewhat different from normal mucous cells morphologically. 5. According to damage of surface mucous cells, cytoplasmic PAS-positive granules were markedly decreased, but the PAS-positivity was also restored with restitution of mucous cells(even if weaker reaction than in normal mucous cell). It is thought that absolute ethanol-induced mocosal damage is striking even if the exposure time is brief, and epithelial restitution is achieved for 30 or 60 minutes after ethanol exposure. The mechanism of epithelial restitution might be resulting from migration of undamaged epithelial cells.