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      • 틸라피아의 해수순치에 관한 생리학적 연구 I. 내분비학적 변화

        윤종만,조갑민,박홍양 한국동물번식학회 1993 Reproductive & developmental biology Vol.16 No.4

        This study was taken to examine external changes, behavioral changes, and endocrine changes such as estradiol-17$\beta$, progesterone, T4 and T3 of female Oreochromis niloticus living in 0$\textperthousand$, 10$\textperthousand$, 20$\textperthousand$, and 30$\textperthousand$ salt concentrations, respectively. The results obtained in these experiments were summarized as follows. In seawater obtained in these experiments were summarized as follows. In seawater challenge test, any fish didn't die in each group such as 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, external body color of fish changed from dark-striped to light-grey color. At the same time, thyroxine and triiodothyronine concentrations significantly(P<0.05) increased, and then were at the highest level in 30 salinity. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, feed intake of fish started from the fourth day. From 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, estradiol-17$\beta$ levels were increased gradually. When fish was adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, the levels of each progesterone didn't show significant change, and especially showed the lowest peak in 20$\textperthousand$. The greatest thyroxine activity(T4) was observed in 30$\textperthousand$. The levels of and triiodothyronine(T3) significantly changed in all salinities, and its level was at the highest peak in 30$\textperthousand$ salinity. Correlation coefficients between serum progesterone and triiodothyronine in 10$\textperthousand$ and 30$\textperthousand$ were +0.677 and +0.843, respectively. Correlation coefficient of serum thyroxine(T4) and triiodothyronine(T3) individuals in 10$\textperthousand$ was +0.768, and +0.843, respectively.

      • KCI우수등재

        틸라피아의 해수순치에 (海水馴致) 관한 생리학적 연구 Ⅳ. 광학현미경적 관찰

        윤종만(J . M . Yoon),조갑민(K . M . Cho),박홍양(H . Y . Park) 한국축산학회 1992 한국축산학회지 Vol.34 No.6

        This study was taken to examine the light microscopic changes in gill, liver, kidney of female tilapia (Oreochromis niloticus) after adapting in 0‰, 10‰, 20‰, and 30‰ salt concentrations, respectively. Gill chloride cell hyperplasia, gill lamellar epithelial separation, glomerular shrinkage, congestion in kidneys and deposition of hyalin droplets in kidney glomeruli, and tubules were observed in treated fished with saline. Incidence and severity of gill chloride cell hyperplasia and the number of chloride cells were rapidly enhanced with increasing the salinity. These results suggest to excrete sodium chloride and inorganic components accumulated in the body of a fish and to balance osmotic pressure with reference to progressive increase of osmolality. Also the increased number of gill chloride cell seemed to be to maintain homeostasis. However, the separation of the gill lamellar epithelium was severer in the higer saline water. In the seawater-adapted fish, the hepatic cells became somewhat enlarged and some round vacuoles appeared within the cytoplasm. Glormerular shrinkage was occurred higher in individuals in 10‰, 20‰, 30‰ seawater than in those in freshwater. Glomeruli appeared to contract in kidneys in 14 days following transfer of fish from freshwater to 10‰ seawater. The size of kidney glomeruli in individuals at 10‰, 20‰, and 30‰ saline showed smaller than that at 0% saline. These cytometrical changes may be related to the different osmoregulatory functions of euryhaline teleosts. Our findings demonstrated that tilapia tolerated moderately saline environment and the increased body weight with increasing salinity, regardless of histopathological changes.

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