곤충다양성의 이용과 보전,복원 : 심포지엄 ; 곤충자원을 활용한 함평나비축제와 2008함평세계나비,곤충엑스포

정헌천 한국응용곤충학회 2006 한국응용곤충학회 학술대회논문집 Vol.2006 No.9

곤충자원을 활용한 함평나비축제와 2008함평세계나비.곤충엑스포

정헌천 한국응용곤충학회 2006 한국응용곤충학회 학술대회논문집 Vol.2006 No.1

Characterization of optimal growth conditions and carotenoid production of strain Rhodotorula mucilaginosa HP isolated from larvae of Pieris rapae

오주경,정헌천,오세종 한국곤충학회 2009 Entomological Research Vol.39 No.6

Yeasts have been studied because of their production of a pigment known as carotenoid with potential application in food and feed supplements. A carotenoid-producing yeast was isolated from the larvae of Pieris rapae, named HP. The strain HP was identified as Rhodotorula mucilaginosa classified by its carbohydrate fermentation pattern and physiological tests. Rhodotorula mucilaginosa HP produces several exogenous enzymes: alkaline phosphatase, esterase, leucine arylamidase, valine arylamidase, acid phosphatase and β-glucosidase. Using response surface methodology, selected medium components (yeast extract, malt extract, peptone, glucose) were tested to find the optimum conditions for carotenoid production and the growth of R. mucilaginosa HP. Central composite design was used to control the concentrations of medium components. Peptone and glucose had the largest effects on carotenoid production and cell growth of R. mucilaginosa HP, respectively. The estimated optimal growth conditions of R. mucilaginosa HP were: yeast extract 3.23%, malt extract 2.84%, peptone 6.99% and glucose 12.86%. The estimated optimal conditions for carotenoid production were: yeast extract 2.17%, malt extract 2.11%, peptone 5.79% and glucose 12.46%. These results will assist in the formulation of an appropriate culture medium for optimal carotenoid production of R. mucilaginosa HP for commercial use.

Evaluation of nutritional status of an edible grasshopper, Oxya Chinensis Formosana

현숙희,권기한,박근형,정헌천,권오석,Hamisi Tindwa,한연수 한국곤충학회 2012 Entomological Research Vol.42 No.5

This study was conducted to examine the nutritional status of the grasshopper (Oxya chinensis formosana, OCF) as human food, exploring it as an alternative edible resource. Analysis of free amino acid shows that there are various essential amino acids in addition to saturated and unsaturated fatty acids in OCF dried powder. Analysis of the mineral contents and vitamins of dried OCF indicates that it is rich in calcium, vitamin B6, and niacin. The heavy metal content of OCF recorded was low, making it safe for human consumption: OCF had plumbum at 0.01–0.03 mg/100 g, cadmium at 0.002–0.005 mg/100 g, arsenic at 0.07–0.17 mg/100 g, and mercury at 0.0003–0.0007 mg/100 g. In conclusion, given its high nutritive quality in terms of proteins and fats, coupled with lower heavy metal content, it would be recommended to use the grasshopper (OCF) as substitute to the traditional sources of protein.

Expression analysis and immunohistochemical localization of putative tumor suppressor QM homologue from the cabbage butterfly, Pieris rapae

박보미,Bharat Bhusan Patnaik,오승한,김동현,조용훈,정헌천,이용석,고기성,김익수,한연수 한국곤충학회 2013 Entomological Research Vol.43 No.5

The tumor suppressor, QM has been cloned and characterized from eukaryotic organisms including humans, vertebrates, invertebrates, plants and yeast. However, no study on Pieris rapae QM (PrQM) has been reported to date. In this study, cDNA encoding a putative QM protein (PrQM) was obtained from expressed sequence tags (ESTs) of the Pieris rapae cDNA library. Phylogenetic analysis of PrQM showed high similarity of amino acid sequence with other putative homologues identified from Heliothis virescens (95%), Bombyx mori (92%), Plutella xylostella (92%), Drosophila melanogaster (89%) and Polyrhachis vicina (85%), indicating that QM is highly conserved among insects. Semi‐quantitative PCR datasets revealed that PrQM transcripts are highly expressed in head, silk gland, integument and fat body, with pronounced expression observed during the egg stage. The coding region of the PrQM gene was cloned into the pET28 (+) expression vector and the recombinant protein purified by His‐tag affinity chromatography was used for antibody production. Western blotting with the anti‐PrQM antibody detected a single band corresponding to the expected molecular weight of both endogenous (26 kDa) and recombinant (29 kDa) PrQM. Furthermore, immunohistochemical and confocal microscopic analysis with the anti‐PrQM antibody showed that the QM gene is highly expressed in the cytoplasm of fat body, gut and Malpighian tubules in virus‐uninfected control larvae, but down‐regulated at 4 days post Pieris rapae granulovirus (PiraGV) infection. These data show that PrQM is negatively regulated upon virus infection and suggests a putative immunomodulatory function.

Mitochondrial COI sequence-based population genetic analysis of the grasshopper, Patanga japonica Bolívar, 1898 (Acrididae: Orthoptera), which is a climate-sensitive indicator species in South Korea

Jee-Young Pyo,박정선,Seung-Hyun Lee,김성수,정헌천,김익수 한국잠사학회 2023 International Journal of Industrial Entomology Vol.47 No.2

Patanga japonica Bolívar, 1898 (Orthoptera: Acrididae) is listed as a climate-sensitive indicator species in South Korea and is called southern group of insects in that the main distributional range is southern region of South Korea and Asian continent. In South Korea, thus, the species was distributed mainly in southern region of South Korea including southward a remote Jeju Island, but recently the species has often been detected in mid to northern region of South Korea, implying northward range expansion in response to climate change. Understanding the characteristics of the changes in genetic diversity during range expansion in response to climate change could be a foundation for the understanding of future biodiversity. Thus, in this study, we attempted to understand the changing pattern of the genetic diversity of the P. japonica in newly expanded regions. For the purpose of study, we collected 125 individuals from seven localities throughout South Korea including two newly distributed regions (Pyeongtaek and Yeongwol at ~37° N). These were sequenced for a segment of mitochondrial cytochrome oxidase subunit I (COI) and analyzed for genetic diversity, haplotype frequency, and population genetic structure among populations. Interestingly, northward range expansion accompanied only haplotypes, which are most abundant in the core populations, providing a significant reduction in haplotype diversity, compared to other populations. Moreover, genetic diversity was still lower in the expanded regions, but no genetic isolation was detected. These results suggest that further longer time would take to reach to the comparable genetic diversity of preexisting populations in the expanded regions. Probably, availability of qualified habitats at the newly expanded region could be pivotal for successful northward range expansion in response to climate change.

RNA Sequencing, De novo assembly, functional annotation and SSR analysis of the endangered diving beetle Cybister chinensis (= Cybister japonicus) using the Illumina platform

황희주,Bharat Bhusan PATNAIK,강세원,박소영,정종민,상민규,박지은,민혜린,성지연,조용훈,노미영,이종대,정기윤,박홍석,정헌천,이용석 한국곤충학회 2018 Entomological Research Vol.48 No.1

Cybister chinensis Motschulsky, 1854 (synonym Cybister japonicus Sharp, 1873) is a beetle found in ponds and irrigation canals near rice fields regulating the aquatic faunal community through predation. However, due to loss of natural habitats, use of pesticides, and invasion of alien species the beetle is threatened. With lack of understanding at the trophic ecology and genomics level, the conservation study is hindered to a larger extent. In the present study, Illumina HiSeq 4000 platform has been used to unravel the whole‐larval transcriptome of the beetle. A total of 20,129 non‐redundant unigenes were assembled from 67,260,666 clean read sequences. About 18,743 unigenes found a homologous match in any one of the databases like PANM, UniGene, Swiss‐Prot, Clusters of Orthologous Groups (COG), Gene Ontology (GO), KEGG, and InterProScan. While the zinc finger domains topped the unigene hits, about 660 enzymes (2695 sequences) participating in metabolism, environmental information processing, genetic information processing and organismal system pathways were recorded. Furthermore, the HSP70 class, Toll‐like receptors 4, insulin‐receptor substrate, and AMP activated protein kinase showed conspicuous presence in the larval transcriptome. Out of a total of 12,491 unigene sequences examined, 1968 SSRs were detected. Majority of them were dinucleotide repeats with six iterations followed by trinucleotide and tetranucleotide repeats with five and four iterations, respectively. This is the first report of cDNA resources from C. japonicus till date. The data would be crucial for the assessment of the beetle in the wild and making an inventory for utilisation in future genomics and ecological studies.

RNA sequencing, de novo assembly, and functional annotation of an endangered Nymphalid butterfly, Fabriciana nerippe Felder, 1862

황희주,Bharat Bhusan Patnaik,강세원,박소영,왕태훈,박은비,정종민,송대권,Hongray Howrelia PATNAIK,김창무,김순옥,이재봉,정헌천,박홍석,한연수,이용석 한국곤충학회 2016 Entomological Research Vol.46 No.2

Grassland butterflies are considered representative indicators of biodiversity and ecosystem health. Their dramatic decline caused by habitat destruction, intensifying agriculture and global warming has prompted concern for conservation. While ecological indices of butterflies have been documented, there is a lack of genomic resources relative to the biological importance of this group. Here, we report, first whole‐transcriptomic resource for Fabriciana nerippe, a brush‐footed butterfly member that is endangered in Korea. Approximately, 241.3 million clean reads were obtained from paired‐end Illumina sequencing of adult whole‐body RNA. The de novo assembly resulted in 114 405 unigenes with length ranging from 133 to 33 218 bp. We found 41 868 assembled unigenes homologous to sequences in locally curated PANM‐DB (Protostome DB). We assigned gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology terms to 18 085 and 5779 unigenes, respectively. InterProScan predicted 6691 protein domains for the assembled unigene sequences, with the reverse transcriptase and zinc finger domains found to be the most predominant. A total of 315 282 simple sequence repeats (SSRs) were identified from the assembled unigenes, when considering dinucleotide to octanucleotide repeats with a minimum of three repeat units. AT/AT, AAT/ATT, and AAAT/ATTT represented the most prominent SSR repeat types in the transcriptome. The unigene annotation profile and microsatellites generated in the study can serve as a reference resource for closely related species and gene functional analysis studies. Our data can be utilized to study ecological drift and loss of the species, consequently leading to better protection of the population.