히스톤 단백과 핵과의 결합

정용자 慶星大學校 1988 論文集 Vol.9 No.1

HISTONE은 CYTOPLASM에서 합성되어 진핵 세포의 염색체와 결합하여 있다. 그러나 이들 PROTEIN의 핵막을 통한 이동에 관해서는 거의 알려져 있지 않다. HeLa CELL에서 합성후 곧 이행됨을 나타냈으며 (1967 ROBGIN등), HISTONE은 그것이 양이돈 CHARGE를 가지고 있디 때문인지, 아니면 특별한 TRANSPORT SYSTEM이 관여함으로써 이행하는 것인지을 알기 위해서 RAT LIVER에서 핵을 분리하여 분리한 핵과 ??C-HISTONE을 결합시킨후 (BINDING), 또다시 그 BINDING된것을 SDS 전기영동에 의해 해리되지 않도록 CROSS-LINKER를 사용해서 (DSP) 안정결합(CROSS LINKING)을 시킨 후 ELECTROPHORESIS와 RADIOGRAM에 의해 안정결합(CROSS LINKING)을 시킨후 ELECTROPHORESIS와 RADIOGRAM에 의해 함유 PROTEIN을 CECK함으로서 우선 HISTONE과 NUCLEL가 결합되는 여부의 실험을 행함. 실험결과 : HISTONE과 NUCLEI는 INCUBATION PERIOD와 농도에 따라 결합속도와 범위에 관여하며, 온도에 따라 다르다. HISTONE과, NUCLEISMS DITHIOBIS(SUCCINIMIDYL PROPIONATE)의 존재하에서 핵 PROTEIN과 결합하여 RADIOGRAM에서 BINGING BAND가 나타났음. Interactions between protein components of rat liver nuclei and histone were investigated by using cross-linking reagent, Dithiobis (succinimidyl propionate). The rate and extent of binding was a function of the incubation period and the concentration of histone and nuclei, but independent of the temperature.

식품에 관련하는 미생물상의 위해 및 식품 위생상의 문제점

鄭容子 亞成出版社 1979 釜山女子大學 論文集 Vol.1 No.-

The microbial multiplication & the contamination of the food have greatly affected human health. Therefore the quality & freshness of the food become an issue in the epidemiologic problem & the food habits and the protection of the consumer health in consideration of the many conditions participating the growth & exsistence of the microorganism, and the production of the toxin. The major methods of the contamination prevention in food microorganism are as follows. Salmonella group: Block the contamin ation route from the animal to the man because the cycle is among man, animal and environment. Shigella: Block the contamination route from stool because the man is only carrier. Bacterium coli neapolitanum: preventive measure of food contanination & control of the microbial multipication of the food. Vibrio cholera: It has direct connection, and ha ve relation to preventive knowledge. Vibrio parahaemolyticus: Sea food is causative food, its prevention is forbidden vibrio from multiplication in sea food. Clostridium group: Stored food, especially the food of storage life in household affairs is causative food. Its prevention is heating & cooking, and storage required rapid cooking after heating. Fungus & mycotoxin: Fodder need prevention of production of mycotoxin and its establish the method in its harvest, storage and transportation parasites: Best prevention knows whether parasites contamination slaughtering animals. Transmission of drug tolerance intestinal bacteria: A used antibiotics in fodder has possibility that drug tolerance spread from animal to man. Therefore abuse of antibiotics should prohibit in animals. The general run of people need the distribution of information on matters of environ mental hygiene for examples ① microbial multiplication & toxin production due to stability dealing with food , food manufacture, processing & storagc. ② the microbiologic injury in relation to food & countermeasure of prevention. Sanitary distribution of food needs strict hygiene inspection.

酸性色素에 依한 Prifinium Bromide의 溶媒抽出分光光度 定量法

安文圭,鄭容子 경성대학교 1985 부산수산대학 논문집 Vol.6 No.2

Ion pair compounds between prifinium bromide and acid dyes were extracted from the aqueous solution into organic phase and determined colorimetrically each of the maximum absorption wavelength. Diprotic acids such as Bromphenol blue, Bromcresol green, Bromthymol blue and monoprotic acids such as tetrabromo phenolphthalein ethyl ester and dichlorophenol indophenol were used as color forming agents. The intensity of the color was measured spectrophotometrically. The absorbance showed a linear relationship to the concentration of prifinium bromide in the aqueous solution over the range of (1∼5)×?? M for BPB, (2∼6)×?? M for BCG and BTB, and (1∼5)×?? M for TBPE and DCIP. A singly charged TBPE and DCIP anion was found to be extracted with prifinium bromide as 1:1 associates in organic phase, while in the case of diprotic acid dyes was extracted 2:1 associates. The analysis was carried out as follows; buffer solution 5㎖, acid dye solution (5×10??) 3㎖and prifinium standard solution (5×10??) (1∼5)㎖ are poured into a sep aratory funnel. After diluting the mixture to 50㎖ with water, 10㎖ of chloroform is added. After the solution shaked for 3 min, the organic phase is centrifuged to remove droplets of water. The absorbance of the extracts is measured at each maximum absorption wavelength, using a reagent blank or water as reference.

Hepatoma cell에 증식억제작용을 나타내는 식물들

이은주,정용자 慶星大學校 1998 論文集 Vol.19 No.1

We have studied that the effect on the inhibition of the cancer cell growth by the 3-[4,5-Dime wthylthiazol-2 yl]-2,5-diphenyl-tetrazolium bromide(MTT) assay, to investigate the anticancer effect with plants, observed around us, by culturing the human hepatoma cell-line. And we knew it was the best condition that the cell number was 4×10³ cells/well and the time of culture is 48 hours. We found that Equisetum arvense L. and Lactuca dentata Makino. var. Flaviflora Makino. exhibited a good anticancer effect and especially Capsicum annum L. angulosum Mill exhibited the best effect of them.

MCF-7 cell에 미치는 여러 가지 식물들의 항-증식 효과

곽수영,정용자 慶星大學校 1999 論文集 Vol.20 No.2

The anti-proliferating effects of some plants on the MCF-7 cells were studied by the 3-[4,5-Dimethylthiazol-2yl]-2,5-diphenyl-tetrazolium bromide(MTT assay), to investigate the anticancer affect with some plants. Equisetum arvense L. and Lactuca dentata Makino. var. Flaviflora Makino of them relatively showed a good anticancer effect. Capsicum annuum L. var. angulosum Mil was the best among them. We also examined morphological changes on the MCF-7 cells in this process. The cells included in medium were investigated to be aggregated and destroyed slowly by treatment with Lactuca dentata Makino. var. Flaviflora Makino. In case of Capsicum annuum L. var. angulosum Mill, the cells became vague after 2 days, and then destroyed faster than others.

MCF-7에 미치는 인근식물들의 항암효과 Screening Test

곽수영,이은주,정용자 慶星大學校 1998 論文集 Vol.19 No.1

We have studied that the effect on the inhibition of the cancer cell growth by the 3-[4,5-Dimewthylthiazol-2 yl]-2,5-diphenyl-tetrazolium bromide(MTT) assay, to investigate the anticancer effect with plants, observed around us, by culturing the human MCF-7 mammary gland adenocarcinoma cell-line. And we knew it was the best condition that the cell number was 4×10³ cells/well and the time of culture is 48hours. We observed the effect on the inhibition of the cuncer cell growth with plants, observed us and we can find that Capsicum annum L. angulosum Mill exhibited a good anticancer effect.

식품에 관련하는 미생물상의 위해 및 식품 위생상의 문제점

鄭容子 부산여자대학 1979 釜山女子專門大學 論文集 Vol.1 No.-

The microbial multiplication & the contamination of the food have greatly affected human health. Therefore the quality & freshness of the food become an issue in the epidemiologic problem & the food habits and the protection of the consumer health in consideration of the many conditions participating the growth & exsistence of the microorganism, and the production of the toxin. The major methods of the contamination prevention in food microorganism are as follows. Salmonella group: Block the contamin ation route from the animal to the man because the cycle is among man, animal and environment. Shigella: Block the contamination route from stool because the man is only carrier. Bacterium coli neapolitanum: preventive measure of food contanination & control of the microbial multipication of the food. Vibrio cholera: It has direct connection, and ha ve relation to preventive knowledge. Vibrio parahaemolyticus: Sea food is causative food, its prevention is forbidden vibrio from multiplication in sea food. Clostridium group: Stored food, especially the food of storage life in household affairs is causative food. Its prevention is heating & cooking, and storage required rapid cooking after heating. Fungus & mycotoxin: Fodder need prevention of production of mycotoxin and its establish the method in its harvest, storage and transportation parasites: Best prevention knows whether parasites contamination slaughtering animals. Transmission of drug tolerance intestinal bacteria: A used antibiotics in fodder has possibility that drug tolerance spread from animal to man. Therefore abuse of antibiotics should prohibit in animals. The general run of people need the distribution of information on matters of environ mental hygiene for examples ① microbial multiplication & toxin production due to stability dealing with food , food manufacture, processing & storagc. ② the microbiologic injury in relation to food & countermeasure of prevention. Sanitary distribution of food needs strict hygiene inspection.

수종 생약의 Hepatoma cell line에 미치는 형태적 변화의 동정

이은주,정용자 慶星大學校 1998 論文集 Vol.19 No.2

The cells included in medium were invastigated to be aggregated and destroyed slowly by treatment with Lactuca dentata Makino. var. flaviflora Makino. On the other hand, the cells treated with Equisetum arvense L were aggregated to be coat-like. The case of Capsicum annuum L. var. angulosum Mill, the cells became vague after 2 days and then destroyed. This procedure was faster than other. The chromasome was more condensated after 37hr treated with Capsicum annuum L. var. angulosum Mill.

Antitumor and Immunological Effects of Bovine Thymic Extract

정용자,홍기태,정현옥,Chung, Yong Za,Hong, Gi Tae,Jung, Hyun Ok Korean College Of Clinical Pharmacy 1992 한국임상약학회지 Vol.2 No.1

Thymic extract showed antitumor effect to sarcoma mice with higher dose$(200{\mu}g/mouse/day$ i.p., 4weeks) but not with low dose$(5{\mu}g/mouse/day$ i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. $\%$ absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and $G_2/M$ phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to $L_2T_4$ were increased after administration of thymic extract$(2-200{\mu}g/mouse/day)$. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

소 흉선 추출물의 암세포 증식 및 림프구에 미치는 영향

정용자,홍기태,정현옥 한국임상약학회 1992 한국임상약학회지 Vol.2 No.1

Thymic extract showed antitumor effect to sarcoma mice with higher dose i.p., 4weeks) but not with low dose i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to were increased after administration of thymic extract. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.