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넙치, Paralichthys olivaceus에 nalidixic acid, piromidic acid, oxolinic acid의 경구투여 약물동태에 미치는 수온의 영향
정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ),서정수 ( Jung Soo Seo ),최동림 ( Dong Lim Choi ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
퀴놀론계 항균제인 nalidixic acid(OA), piromidic acid(PA), oxolinic acid(OA)를 사육수온(13±1.5℃, 23±1.5℃)에 따라서 넙치(평균체중 700g)에 60㎎/㎏의 농도로 1회 경구 투여한 다음, 경시적인 혈청내 잔류농도를 분석하였다. 수온이 23±1.5℃의 경우, 투여 후 NA는 10시간째 (11.55㎍/㎖), PA는 24시간째(3.79㎍/㎖), OA는 30시간째(1.12㎍/㎖)에 각각 최대혈중농도에 도달하였다. 수온이 13±1.5℃의 경우, 투여 후 NA는 10시간째(6.36㎍/㎖), PA는 15시간째(1.4㎍/㎖), OA는 30시간째(1.01㎍/㎖)에 각각 최대혈중농도에 도달하였다. NA와 PA의 넙치 혈중내 흡수정도는 수온 13±1.5℃보다 수온 23±1.5℃에서 매우 높게 나타났으며, NA의 넙치 혈중내 소실정도는 수온 13±1.5℃보다 수온 23±1.5℃에서 두드러지게 빨랐다. 한편 OA의 넙치 혈중내 흡수 및 소실정도는 사육수온에 크게 영향을 받지 않는 것으로 나타났다. NA 및 PA는 one-compartment model, OA는 two-compartment model로 해석하여 WinNonlin program을 이용, 약물동태학적 매개변수(parameter)를 조사하였다. 수온이 23±1.5℃의 경우, 혈장농도-시간곡선하 면적(AUC)은 NA, PA, OA가 각각 258.26, 248.12 및 138.20㎍?h/㎖, 혈중최고농도의 도달시간(Tmax)은 10.67, 21.15 및 23.95 h, 혈중최고농도(Cmax)는 8.91, 3.09 및 1.06㎍/㎖로 계산되었다. 수온이 13±1.5℃의 경우, AUC는 NA, PA, OA가 각각 341.45, 103.89 및 159.10㎍?h/㎖, 혈중최고농도의 도달시간은 7.72, 12.89 및 28.03 h, 혈중최고농도는 6.23, 1.22 및 1.02㎍/㎖로 계산되었다. Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of nalidixic acid (NA), piromidic acid (PA) and oxolinic acid (OA) were studied after oral administration to cultured olive flounder, Paralichthys olivaceus. Serum concentrations of these antimicrobials were determined after oral administration of a single dosage of 60㎎/㎏body weight (average 700g). At 23±1.5℃, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 24 h and 30 h post-dose, were 11.55, 3.79 and 1.12㎍/㎖, respectively. At 13±1.5℃, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 15 h and 30 h post-dose, were 6.36, 1.4 and 1.01㎍/㎖, respectively. Better absorption of NA and PA was noted at 23±1.5℃ compared to 13±1.5℃. The elimination of NA from serum of olive flounder was considerably faster at 23±1.5℃ than at 13±1.5℃. However, both absorption and elimination of OA were not affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of these antimicrobials in serum were analyzed by fitting to a one-and two compartment model, with WinNonlin program. In the one compartment model for NA, AUC, Tmax and Cmax at 23±1.5℃ were 258.26㎍·h/㎖, 10.67 h and 8.91㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 341.45㎍·h/㎖, 7.72 h and 6.23㎍/ ㎖, respectively. In the one compartment model for PA, AUC, Tmax and Cmax at 23±1.5℃ were 248.12㎍·h/㎖, 21.15 h and 3.09㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 103.89㎍·h/ ㎖, 12.89 h and 1.22㎍/㎖, respectively. In the two compartment model for OA, AUC, Tmax and Cmax at 23±1.5℃ were 138.20㎍·h/㎖, 23.95 h and 1.06㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 159.10㎍·h/㎖, 28.03 h and 1.02㎍/㎖, respectively.
Sulfadimethoxine의 경구 투여에 따른 넙치, Paralichthys olivaceus 혈액 및 간에서의 잔류량 변화
정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.2
The residue levels of sulfadimethoxine (SDM) was studied after oral administration to cultured olive flounder, Paralichthys olivaceus at 20±1.0℃. The concentrations of SDM in the plasma and liver were determined by HPLC-UV detector after a single dosage of 400 ㎎/㎏body weight. The average recoveries of SDM in spiked samples between 2~50 ppm were 92.24~93.62% for plasma and 88.34~91.90% for liver. Limit of detection for SDM was 0.05 ppm by using this method. Samples were taken at 1 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h, 240 h, 336 h and 480 h post-dose. The peak plasma and liver concentrations of SDM, which attained at 1 h post-dose, was 402.64±59.66 ㎍/㎖and 238.18±54 ㎍/g, respectively. Thereafter, it`s elimination from both tissues was considerably faster following process of time. Their concentrations of SDM were not measurable at 480 h post-dose. Based on this results, dosage and withdrawal times for SDM could be used when it is prescribed with SDM in olive flounder.
잔류 항균물질에 대한 미생물학적 간이검사법의 검출감도 비교
정승희(Sung Hee Jung),김진우(Jin Woo Kim),손상규(Sang Gyu Son) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3
Minimun-detectable levels to 28 antibacterial agents used for the prevention and the treatment of fish diseases were determined to establish optimal detective method of bioassay in fish by the EEC 4-plate method, the modified method of EEC 4-plate and the standard method of analysis in food safety regulation. The test organisms used in the methods of bioassay were as follows: Bacillus subtilis BGA(B. subtilis)and Micrococcus luteus ATCC 9341(M. luteus) in the EEC 4-plate method, B. subtilis, M. luteus, and Bacillus cereus var. mycoides ATCC 11778(B. cereus) in the EEC 4-plate and B. subtilis, M. luteus, B. cereus and Bacillus stearothermophilis var. calidolactis C-953 (B. starothermophilis)in the standard method. The standard method showed predominant sensitivity in the detection of penicillins(PCs), and was also highly sensitive to aminoglycosides (AGs). The sensitivity of standard method in the detection of tetracyclines (TCs), macrolides(MLs), nitrofuran derivatives(NFs) and quinolones(Qns) was very low, and against sulfonamides (SAs), however, was extremely low. The modified method of EEC 4-plate showed very high sensitivity to TCs. Both the EEC 4-plate and the modified method of EEC 4-plate showed competitively high sensitivity in the detection of PCs, MLs, NFs, QNs and SAs. All the methods studied in the experiment showed very low sensitivity against chloramphenicol (CMs). Consequently, the modified method of EEC 4-plate was the best bioassay method with a wide .range of sensitivity for the optimal detection of the residual antibacterial agents in fish.
담수산 양식어류의 혈액검사에 사용된 건식 자동생화학 분석기 (FUJIDRI-CHEM 3000) 의 활용 가능성
정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),김진도 ( Jin Do Kim ),최혜승 ( Hye Sung Choi ),박명애 ( Myoung Ae Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.3
The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in cultured freshwater fish and also evaluate application of automatic dry-type chemistry analyzer (FUJI DRI-CHEM 3000) used to those blood tests. A blood profile of total 200 fish for rainbow trout (Onchorhynchus mykiss), israel carp (Cyprinus carpio), tilapia (Oreochromis niloticus) and eel (Anguilla japonica) cultured in Inland Fisheries Research Institute of NFRDI was determined by hemoglobin (Hb) and plasma chemistry tests: total protein (TP), albumin (ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), triglyceride (TG), total cholesterol (TCHO), creatinine (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose (GLU). The values of ALT, TG, LDH, ALB, TCHO, AST and ALP were outside from the minimum and/or maximum of the established detectable range of the analyzer. ALT and TG were not detectable in the range of 67%∼61.5%. LDH, ALB and TCHO were not detectable in the range of 36∼17%. AST and ALP were not detectable in the range of 5.5∼0.5%. However, the values of BUN, CRE, GLU, Hb and TP were below the detectable limits of the analyzer.