한국산 일월비비추 자연집단의 유전적 구조

정명기 (Myong Gi Chung) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.3

I investigated levels of genetic diversity, population genetic structure, and gene flow in Hosta capitata, a herbaceous perennial native to South Korea and southwestern Japan. Starch gel electrohoresis was conducted on leaves collected from 310 plants in 19 Korean populations. Twenty-two of 25 putative loci examined were polymorphic in at least one population and the mean number of alleles per locus was 1.65. In addition, mean expected heterozygosity within populations (Hep=0.153) was higher than average values for species with similar life history traits. Significant differences in allele frequency were detected between populations at all loci (P<0.01), and slightly aver 30% of the genetic variation was found among populations (G_ST=0.308). Indirect estimates of the number of migrants per generation (Nm) (0.506, calculated from G_ST; 0.852, calculated from the mean frequency of ten private alleles) indicate that gene flow is restricted among the isolated Korean populations of H. capitata. Factors contributing to the high levels of genetic differentiation among populations of H. capitata include small and discrete populations, human disturbance, and low frequencies of pollinator foraging behavior.

한국 특산식물의 동위효소 다양성 및 집단유전적 구조 : 2. 흑산도비비추 ( 백합과 )

정명기(Myong Gi Chung),정효기(Hye Gi Chung) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.2

Levels of genetic diversity, population genetic structure, and gene flow in Hosta yingeri, a herbaceous perennial endemic to Taehuksan, Sohuksan, and Hong Islands, were investigated. Starch gel electrophoresis was conducted on leaves for 101 plants collected from three populations. Although the distribution of the spacies is restricted in the islands, it maintains high levels of genetic variation; 64% of polymorphic loci in at least one population (P_s), the mean number of alleles per locus (A_p) of 1.92, and the mean effective number of alleles per locus (Ae_p) of 1.52. Overall, mean genetic diversity (He_p=0.250) was substantially higher than mean estimate for species with very similar life history traits (0.102). Large population size, the persistence of multiple generations withing populations, high fecundity, predominantly outcrossing breeding system, large size of pollinator visitation areas may be explanation factors contributing the higher level of genetic diversity maintained within populations. Analysis of flxation indices showed an overall slight excess of heterozygotes (mean F_IS = -0.066) relative to Hardy-Weinberg expectations, which may in part be due to the near self-incompatible breeding system in the species. Significant differences in allele frequencies among populations were found for 14 out of 16 polymorphic loci ( P<0.05). Slightly more than 80% of the total variation in the species was common to all populations (G_ST = 0.198). As expected, indirect estimate of the number of migrants per generation (Nm = 0.45, calculated from mean G_ST) and nine private alleles found in the three populations indicate that gene movement among three isolated island populations was low.

한국산 갯메꽃 및 메꽃 ( 메꽃과 (科) ) 집단의 유전적 다양성 보존에 대한 암시

정명기(Myong Gi Chung),김승태(Seung Tae Kim),정효기(Hye Gi Chung),정문수(Mun Su Chung) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2

N/A

Geographic Spatial Autocorrelation of Morphological Characters among Korean Populations of Hemerocallis thunbergii Baker

Park Ki-Bae(박기배),Chung Myong-Gi(정명기),Park Joong-Choon(박중춘) 한국원예학회 1999 Horticulture, Environment, and Biotechnology Vol.40 No.4

한국산 돌콩 자연집단의 유전적 다양성

정수동(Su Dong Chung),허홍욱(Hong Wook Huh),정명기(Myong Gi Chung) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.1

N/A

한국산 갯무 자연집단의 유전적 다양성

허만규(Man Kyu Hur),허홍욱(Hong Wook Huh),정명기(Myong Gi Chung) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4

N/A

Allozyme Diversity and Population Genetic Structure in Host a Jonesii(Liliaceae), a Korean Endemic Species

정명기 한국유전학회 1994 Genes & Genomics Vol.16 No.2

Levels of genetic diversity, population genetic structure, and gene flow in Hosta jonesii, a herbaceous perennial endemic to several islands in southern Korea, were investigated. Starch-gel electrophoresis was conducted on leaves collected from 93 plants in four populations. Nineteen of 25 putative loci examined were polymorphic (P_s=76%) in at least one population and the mean number of alleles per locus was 2.02. In addition, mean expected heterozygosity within populations (He_p=0.273) were higher than average values for species with similar life-history trails. Significant differences in allele frequencies were detected between population at 16 loci (P<0.05), and slightly more than 10% of the genetic variation was found among populations (G_(ST)=0.109). Indirect estimate of the number of migrants per generations (Nm) was 1.15, calculated for G_(ST), which indicates that gene flow is high among four populations of H. jonesii. Large population size, predominantly outcross-animal breeding system, seed dispersal by wind, and relatively undisturbed island habitats may result in high levels of genetic diversity within populations and low levels of genetic differentiation among population.

한국산 재배무의 allozyme 의 다양성

정명기,허홍욱,허만규 한국유전학회 1995 Genes & Genomics Vol.17 No.3

Allozyme study in Korean cultivated radish, Raphanus sativus L. var. hortensis Backer f. acanthiformis Makino, was conducted to estimate the levels and distribution of genetic variation among populations using starch gel electrophoresis. Populations of cultivated radish show moderate levels of allozyme variation; the mean percentage of polymorphic loci was 39.4%, mean number of allales per locus was 1.52, and mean expected heterozygosity was 0.079. It is probable that a combination of outcrossing breeding system, high fecundity, and cultivated status may in part be explanatory factors contributing a moderate level of genetic diversity within populations. An overall slight deficiency of heterozygotes relative to Hardy-Weinberg expectations (mean F_(IS)=0.076) indicates that consanguineous mating occur within cultivated radish populations. Of the total genetic variation found in cultivated radish populations examined, only 7% is due to differences among populations (mean G_(ST)=0.073). It is supposed that the cultivated status of radish may influence the considerably low population differentiation. In addition, seed dispersal by humans leads to enhanced levels of gene flow and decreased population divergence in Korean cultivated radish.

韓半島 自生 원추리屬(百合科)의 分類學上 註

정문수,정명기 진주산업대학교 농업기술연구소 1995 農業技術硏究所報 Vol.8 No.-

원추리속은 백합과에 속하고 약 30종으로 구성된 원예학적으로 중요한 속이며 동북아시아 특산이다. 19종류의 형태학적 개화학적인 형질을 대상으로 주성분 분석 결과 4종의 한반도 자생 원추리가 인지되고 있다; 백운산원추리(=함양원추리, 중부, 남부지방 및 한반도 북서 지방에 자생) ; 노랑원추리(=H. coreana는 한반도 남서지방 및 중부지방에 자생) ; 들원추리 (한반도 북동부 및 중부 고산지대 자생) ; 홍도원추리(홍도, 대흑산도 및 소흑산도에 자생), 각 종에 대한 형태학 및 생물학적 특질이 본 논문에 기재되고 있으며 앞으로 경제적으로 유용한 원예품종을 작출하기 위해서 형질의 원예학적 유용성에 대해서 제시하고 있다. Hemerocallis L. (Liliaceae) is an horticulturally important genus of approximately 30 species of herbaceous perennial restricted to East Asia. Based on the principal components analyses using 19 morphological and phenological traits in this study, four Korean species have been recognized : H hakuunensis Nakai (=H. micrantha Nakai, growing on the southern, cental, and northwestern Korea) ; H. thunbergii Baker (=H. coreana Nakai, found on the southwestern and central Korea) ; H. middendorffii Tr. et Mey. (the central and northeastern Korea) ; and H. hongdoensis M. Chung & S. Kang (Hong. Taehuksan, and Sohuksan Islands). The morphological and ecological traits for each species and the horticultural usefulness of these characters to produce economically important cultivars are described.

Genetic Diversity in Korean Populations of Glycine soja (Fabaceae)

Chung, Su Dong,Huh, Hong Wook,Chung, Myong Gi 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

자가수분이 주된 교배계인 돌콩은 1년생 초본이며 대두의 품종개량을 위한 생식질원으로 이용되어 오고 있다. 본 연구에서는 전분 전기영동법을 사용하여 획득한 한반도내 22군데 자연집단내 · 간에서의 유전적 변이의 수준과 분포를 기술하고 있다. 이 종이 지니는 집단내에의 유전적 변이도는 대부분의 다른 일년생 초본에서 보이는 수준이었다. 예를 들면, 집단내 평균 유전좌위의 다형성(P)은 32.6%를 보였고 유전좌위당 대립인자의 수(A)는 1.32였으며 집단내 평균 유전적 다양도(H_e)는 0.112를 나타내었다. 또한 다형성 유전좌위만 고려한 전체 유전적 다양도는 0.347이었다. 그러나 조사된 모든 다형성 유전좌위에서 집단간의 대립인자의 빈도의 차이가 통계학적으로 유의성이 있는 것으로 나타났으며(P<0.001) 평균적으로 이 종이 지니는 전체 유전적 다양도 중 약 70% 정도가 조사된 모든 집단에 공통으로 나타났다. 평균 G_ST값으로부터 간접적으로 구한 세대당 집단간 이동개체수(N_m=0.58)는 이 종의 한반도내 집단간의 유전자이동은 낮다는 것을 암시하고 있다. 자가수정계수분석에 의하면 이형접합자의 부족이 상당한 수준에서 보였다. 이 이유로는 자가수분이 주된 교배계이며, 제한된 유전자이동, 유전적 부동 및 창시자효과 때문이라고 해석된다. 집단간의 유전적 분기수준이 높은 점을 감안해 볼 때 유전적 변이가 높은 한반도의 동해안 및 남쪽지역의 집단들을 주로해서 몇 군데 집단들이 보존되어야 될 것이다. Glycine soja Sieb. et Zucc., a predominantly selfing annual, has been served as a reservoir of germplasm for soybean, G. max (L) Merr., cultivar improvement. This study describes the levels and distribution of genetic variation within and among 22 Korean populations of G. soja using starch gel electrophoresis. The species maintains very similar levels of genetic variability within populations observed in most other annuals. At the population level, the mean percent of polymorphic loci (P) was 32.6%, mean number of allele per locus (A) was 1.32, and mean expected heterozygosity (H_e) was 0.112. In addition, total genetic diversity (H_T) calculated only for polymorphic loci was 0.347. However, significant differences in allele frequencies among populations were found for all loci (P<0.001 in each case) and, on average, about 70% of the total variation in the species is common to all populations. Indirects estimate of the number of migrants per generation (Nm=0.58, calculated from mean G_ST) indicates that gene flow is low among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygote deficiency in most populations and at all loci. This indicates that most populations sampled may have been substructed largely due to inbreeding (predominantly selfing) and restricted gene flow, coupled with founder effect and genetic dreft. Considering a high genetic divergence among populations, it is recommended that several Korean populations of the species should be preserved, especially such as populations in the eastern and southeastern Korean peninsula with high variation.