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${\delta}-Aminolevulinic$ Acid 생산 광합성세균의 분리 및 배양특성
정대열,최양문,양한철,조홍연,Cheong, Dae-Yeol,Choi, Yang-Mun,Yang, Han-Chul,Cho, Hong-Yon 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
광합성세균을 이용하여 생물제초제의 하나인 ${\delta}-aminolevulinic$ acid(ALA)를 생산할 목적으로 자연계로부터 ALA 생산능력이 우수한 균주를 분리하고 ALA의 최적생산에 미치는 일부 배양특성을 검토하였다. 선정균주 KK-10을 동정한 결과 홍색비유황세균에 속하는 Rhodobacter capsulatus로 판명되었다. 본 균주의 ALA 생산성을 높이기 위하여 ALA 탈수효소의 저해제인 levulinic acid(LA)를 15 mM 농도로 배양중기에서 배양액에 첨가함으로써 ALA의 생산량은 약 20배(28 mg/l) 증가되었다. ALA의 전구물질인 glycine과 succinate를 각각 30 mM 복합첨가할 때 약 50배(73 mg/l)생산량을 나타내었고 전구물질의 첨가에 의해 ALA 합성효소의 생합성량은 2배 증가하였다. 분리균주는 전구물질 함유 배지에 15 mM LA를 대수기 중기부터 4회 연속첨가함으로써 85 mg/l 균체외 ALA를 생산하였다. Screening, Identification and some cultural characteristics of ALA$({\delta}-aminolevulinic\;acid)$-producing photosynthetic bacteria were carried out for the optimal production of ALA, one of the bioherbicides. Among photosynthetic bacteria isolated from soil, marsh, pond, etc., KK-10 was the best producer of ALA and identified to be Rhodobacter capsulatus belonging to a typical group of nonsulfur purple bacteria. By addition of 15 mM LA (levulinic acid), an inhibitor of ALA dehydrase in cyclic tetrapyrrole biosynthesis, into culture broth at middle log phase of cell growths, ALA production was considerably increased to about 20-fold (28 mg/l). The combined supplementation of glycine and succinate, each with a concentration of 30 mM also enhanced production of ALA and activity of ALA synthase to about 50-fold (73 mg/l) and 2-fold, respectively. The isolated strain was able to produce upto 80 mg/l under the cultural condition optimized by addition 15 mM LA into the synthetic medium at four different points starting middle log phase.
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정대열 한국전산구조공학회 1994 전산구조공학 Vol.7 No.4
근본적으로 터널을 정확히 해석하려면 3차원 해석이 필요하다. 그러나 3차원 해석은 다루어야 할 자료가 방대하고 또한 시공 단계를 고려하는 3차원 소성해석을 정확히 하기 위해서는 슈퍼컴퓨터와 같은 컴퓨터 파워가 요구된다. 따라서 터널의 해석에서는 대개 하중분배율법을 사용하여 2차원 해석을 수행하고 있는 실정이다. 그러나 하중분배율법에 의한 2차원 해석의 결과는 선택한 하중분배율법에 의존함으로 정확한 하중분배율을 아는 것이 중요하다. 2차원 터널해석을 정확히 하기 위해서는 본문에 기술한 요건을 갖춘 프로그램의 확보, 하중분배율에 대한 정확한 평가, 측압 계수의 신중한 선택, 주위 지반의 물성에 대한 신중한 평가가 필요하다.
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두부공업폐수에서 Rhodobacter capsulatus KK-10을 이용한 ${\delta}-Aminolevulinic$ Acid의 생산
정대열,최양문,양한철,조홍연,Cheong, Dae-Yeol,Choi, Yang-Mun,Yang, Han-Chul,Cho, Hong-Yon 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
광합성세균을 이용한 고농도 유기폐수인 두부공업폐수의 정화처리와 동시에 두부공업폐수를 생물제초제인 ${\delta}-aminolevulinic$ acid (ALA) 생산용 대체기질로 이용할 목적으로 Rhodobacter capsulatus KK-10을 사용하여 COD 제거율과 ALA 생산능을 검토하였다. 사용된 두부공업폐수의 성상은 pH 4.2, COD/BOD와 BOD/N의 비율은 0.98 및 17.2, BOD : N : P의 함유비율은 10 : 6 : 4, 젖산 함유량 1,080 ppm 등으로 광합성세균의 생육에 적당한 조건이었다. 두부공업폐수에서 본 균주를 4일 동안 배양한 결과 약 94% COD 값의 감소와 1.2 g/l 균체생육을 보였으며 대수기 중기에서 ALA 탈수효소 저해제인 levulinic acid(LA)를 15 mM 첨가한 결과 55 mg/l의 ALA 생산량을 나타내었다. ALA의 전구물질인 succinate 및 glycine과 LA를 각각 15 mM의 농도로 동시 첨가시 최대 114 mg/l의 생산량을 보였다. 상기의 물질들을 각각 15 mM 농도로 연속 첨가한 후 ALA 생성량을 검토한 결과 LA 1회와 glycine과 succinate를 3회에 걸쳐 첨가하였을 때 120 mg/l로 가장 효과적이었으며 COD 제거율은 92%를 나타내었다. The removal efficiency of COD and the production of ${\delta}-aminolevulinic$ acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P=100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/l after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 mg/l. The ALA production was considerably increased to 114 mg/l under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 mg/l and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.
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두부공업폐수에서 Rhodobacter capsulatus KK-10을 이용한 δ-Aminolevulinic Acid의 생산
정대열,최양문,조홍연,양한철 ( Dae Yeol Cheong,Yang Mun Choi,Hong Yon Cho,Han Chul Yang ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
The removal efficiency of COD and the production of δ-aminolevulinic acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P =100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/ℓ after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 ㎎/ℓ. The ALA production was considerably increased to 114 ㎎/ℓ under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 ㎎/ℓ and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.
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δ-Aminolevulinic Acid 생산 광합성세균의 분리 및 배양특성
정대열,최양문,조홍연,양한철 ( Dae Yeol Cheong,Yang Mun Chol,Hong Yon Cho,Han Chul Yang ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
Screening, Identification and some cultural characteristics of ALA(δ-aminolevulinic acid)-producing photosynthetic bacteria were carried out for the optimal production of ALA, one of the bioherbicides. Among photosynthetic bacteria isolated from soil, marsh, pond, etc., KK-10 was the best producer of ALA and identified to be Rhodobacter capsulatus belonging to a typical group of nonsulfur purple bacteria. By addition of 15 mM LA (levulinic acid), an inhibitor of ALA dehydrase in cyclic tetrapyrrole biosynthesis, into culture broth at middle log phase of cell growths, ALA production was considerably increased to about 20-fold (28 ㎎/ℓ). The combined supplementation of glycine and succinate, each with a concentration of 30 mM also enhanced production of ALA and activity of ALA synthase to about 50-fold (73 ㎎/ℓ) and 2-fold, respectively. The isolated strain was able to produce upto 80 ㎎/ℓ under the cultural condition optimized by addition 15 mM LA into the synthetic medium at four different points starting middle log phase.
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정대열,이재환,이용대,예명해 대구대학교 과학기술연구소 2001 科學技術硏究 Vol.8 No.2
The purpose of this study is to discover the architectural type of 「Il-joo-moon」 It deals with 「Il-joo-moon」 that has been built before 1910 in Gyoung-sang province. This study analyzes type of member, jointing method, frame structure method by dividing 「Il-joo-moon」 into four parts such as roof, bracket system, frame structure, podium. The results of this analysis are as follows 1) Roof: The 「Pal-ssac」 style of roof and the 「Matt-bae」 style of roof are dominant and double caves mainly appears. 2) Bracket system: The arrangement of bracket system can be divided into one-line-arrangement and two-line-arrangement. As far as 「Chul-mock-soo」 concerned, 3 or 4 「Chul-mock-soo」 is predominent. 「Tong-jae-gong」 has been used to connect front and back bracket system for some 「Il joo-moon」. When it comes 「jae-gong」, 「Sue-soe」 style, 「Yon-bong」 style and 「Yon-hwa」 style are mainly seen. 3) Frame structure: There are 5 forms in classifying frame structure depending on the jointing method of main-column, diagonal member, sub-column etc. and weight transmission method. 4) Podim: Natural foundation stone is majority. It is judged that 「Il-joo-moon」 has no podium or natural ones, however, it has recently been converted into artificially processed podium in many cases.
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