인삼노두 추출물의 위염 및 위궤양에 대한 효과

정기화,이은방,정춘식,Jung, Ki-Hwa,Lee, Eun-Bang,Chung, Chun-Sik 한국생약학회 1996 생약학회지 Vol.27 No.4

In a preliminary screening of plant extracts for the antigastritic and antiulcer actions in rats, the extracts of head of Panax ginseng Radix showed positive activity in HCl ethanol-induced gastric lesion. Among the systematic fractions of hexane, chloroform, butanol and water, the most potent butanol fraction reduced significantly HCl ethanol-induced gastric lesion at the oral dose of 500 mg/kg. In pylorus ligated rats, hexane and butanol fraction showed decreases in the volume of gastric secretion and acid output, of which effects were stronger in butanol fraction. Further assays with butanol fraction disclosed that it significantly suppressed the aspirin-induced and Shay ulcer. The butanol fraction at the intraduodenal dose of 500 mg/kg showed significant stimulation of mucus secretion.

Improvement of Boar Semen Quality by Sperm Selection Using Magnetic Nanoparticles

KI-Hwa Chung(정기화),Jung-Ho Son(손종호) 한국생명과학회 2016 생명과학회지 Vol.26 No.8

본 연구는 간단히 활용할 수 있는 나노 크기의 마그네틱 비드를 이용하여 정자의 품질을 향상시킬 수 있는지 여부를 규명하기 위하여 실시하였다. 돼지 정자 시료는 인공수정 센터에서 공급받아 실험실로 2시간 이내로 이송한 후 CASA 측정을 통하여 4개의 활력을 나타내는 그룹으로(1, > 90%; 2. 80~90%; 3. 70~80%; 4. < 70%) 분류하였다. 정액은 BTS 희석제를 사용하여 보존하였고, 총 정자수와 동일한 농도의 마그네틱 비드를 정액에 20분간 처리한 후, 5분간 실온에서 마그네틱 비드에 반응한 정자를 자석을 이용하여 분리하였다. 마그네틱 비드 처리 전과 후 정자의 생존율 및 활력은 CASA를 이용하여 측정하였고, 기형율과 정자응집의 정도는 현미경으로 검사하였다. 처리 후의 정자 활력은 4개 그룹 모두에서 유의하게(p<0.0.5) 높은 차이를 보였으며 처리 전에 비하여 평균 7.11% 향상되었다. 정자 활력의 변화는 처리 전 낮은 활력을 보인 그룹에서 보다 현저한 차이를 보였다(< 70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.0.5). 정자 활력을 VCL, VSL, VAP 및 LIN (%)로 구분한 성적에서도 유사한 패턴을 나타냈고, 이러한 현상은 활력 70% 이하를 나타낸 그룹에서 개선 효과가 더욱 뚜렷하였다. 마그네틱 비드 처리 후 정자 생존율은 처리 전에 비하여 평균 4%가 향상 되었고(p<0.0.5), 정자 기형율 또한 3.7~4.5%(p<0.0.5) 정도 감소하였다. 정자 응집의 정도 또한 마그네틱 비드 처리를 통하여 처리 전 낮은 활력을 나타낸 그룹에서 감소됨을 알 수 있었다. The objective of this study was to see if fairly simple magnetic nano-particle treatment enhances boar semen qualities. Boar semen samples were prepared from the swine AI center and samples were divided by 4 different motility groups (1, >90%; 2. 80~90%; 3. 70~80%; 4. <70%) using computer assisted sperm analysis (CASA) evaluation. Boar semen was extended using BTS extender and same number of magnetic nano-particles as total number of spermatozoa in each sample was treated for 20 min and collected for 5 min at room temperature. Sperm qualities such as motility and viability were evaluated by the CASA before and after treatment. Sperm abnormality and degree of agglutination were also evaluated under the microscopic examination before and after treatment. There were significant changes (p<0.05) on sperm motility from all 4 different groups in the average of 7.11% after treatment. The enhancement of sperm motility changes was more clear in the groups of lower sperm motile groups (<70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.05). The sperm motility character in terms of curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) and linearity (LIN, %) showed also similar pattern but motility enhancement wear more clear in below 70% motile group. Average sperm viability was increased to 4% by magnetic nano-particles (p<0.05). The percentage of sperm abnormality was also reduced significantly (p<0.05) to the range of 3.7~4.5% before after treatment. The degree of sperm agglutination was also reduced in lower motility groups by the magnetic nano-particle purification.

승마추출물이 흰쥐의 사염화탄소 유발 간독성에 미치는 효과

정기화(Ki Hwa Jung),정춘식(Chun Sik Chung),노혜림(Hye Rim No) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.1

The effect of Cimicifuga·heracleifolia on CCl₄-induced hepatotoxicity was investigated. Cimicifuga heracleifolia has been used to diaphoresis, antipyretics and detoxification in oriental remedy. We examined the effect of Cimicifuga heracleifolia methanol extract by blood chemical analysis and histopathologic examination. ALT and AST were decreased by 38% and 67% in pretreatment group of Cimicifuga heracleifolia compared to CCl₄ control group respectively. There were significant changes neither in total protein, albumin, triglyceride and cholesterol nor in BUN and creatinine. In histopathologic examination, there were severe necrosis and hemorrhage with infiltration of inflammatory cells around the central vein, zone 3 in the liver of CCl₄ treated rat. Ballooning degeneration of hepatocytes were frequently noted in the periphery of the hemorrhagic necrosis. In Cimicifuga heracleifolia pretreatment group, we observed mild degree of ballooning degeneration and inflammatory cell infiltration. No gross necrosis was observed. We measured malondialdehyde (MDA) formation by TBA method. It showed that the formation of MDA in Cimicifuga heracleifolia pretreatment group was decreased compared to the CCl₄, control group. We got the result of the effect of Cimicifuga heracleifolia on CCl₄-induced hepatotoxicity by decreasing serum ALT and AST. It seems that the decrease of lipid peroxidation is related to the recovery effect.

배합약물(配合藥物)의 생물약제학적(生物藥劑學的) 연구(硏究) ( III ) -Aminopyrine의 해열진통작용(解熱鎭通作用)에 미치는 Atropine Sulfate의 배합효과(配合效果)

박영옥,정기화,김재완,Park, Young-Ock,Chung, Ki-Hwa,Kim, Jae-Wan 한국약제학회 1974 Journal of Pharmaceutical Investigation Vol.4 No.1

The comparative studies were made on the analgesic antipyretic effects of aminopyrine used individually and combined with parasympathetic agents (atropine sulfate). The analgesic antipyretic effects were eximined by Writhing's method to the experimental groups(mouse), and the following effects were found : 1) The active intensity of aminopyrine by it's oral administration combined with atropine sulfate is as follows. Amiinopyrine 80 mg/kg combided with Atropine sulfate 0.005 mg/kg=Aminopyrine 100 mg/kg 2) The most active range of intensity of Atropine sulfate(adjuvant) by it's oral administration is as follows. Atropine sulfate $0.004{\sim}0.005\;mg/kg$.

오리나무 수피엑스의 위염 및 위궤양에 대한 효과

정춘식(Chun Sik Chung),우병희(Byung Hee Woo),이은방(Eun Bang Lee),정기화(Ki Hwa Jung) 한국응용약물학회 1996 Biomolecules & Therapeutics(구 응용약물학회지) Vol.4 No.1

Alnus japonica cortex has been used as antidiarrhea, antihemorrhage and the remedy of indigestion. This study was performed to investigate the effectiveness of the methanol extracts of the Alnus japonica cortex on the gastric lesion and ulcer. The methanol extract was fractionated with hexane, chloroform and butanol, followed by bioassay on antigastritic and antiulcer activity. The methanol extract showed low acute toxicity with minimum lethal dose of more than 5000 ㎎/㎏,p.o. in mice. The chloroform and the butanol fraction reduced gastric lesion in HCl. ethanol induced gastritic model. On gastric secretion in pylorus ligated rat, the hexane and chloroform fraction decreased the volume and acidity. The butanol fraction had significant inhibitory effects on aspirin and Shay`s ulcer. The butanol fraction showed a tendency to inhibit the decrease of mucin secretion due to ingestion of absolute ethanol.

압출성형 산삼배양근의 이화학적 성질 및 침출특성의 비교

한재윤(Jae-Yoon Han),정기화(Ki-Hwa Chung),류기형(Gi-Hyung Ryu) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.8

본 연구에서는 조직배양에 의해 생산된 산삼배양근을 홍삼화하여 부가가치를 향상시키고 새로운 식품소재의 개발을 목적으로 산삼배양근을 압출성형 하였다. 압출성형 산삼배양근의 일반성분, 조사포닌, 말톨, 산성다당체, 페놀성화합물 등의 화학적 성분과 갈색도, 적색도, 침출특성의 물리적 변화를 살펴보았다. 실험결과 주근의 함량이 높은 홍삼이 총당과 환원당 함량은 산삼배양근과 비교하여 높게 나타났으며, 압출성형 산삼배양근의 총당과 환원당은 증가하였다. 반면 미세근으로 이루어진 산삼배양근의 총아미노산 함량이 주근으로 이루어진 홍삼과 비교하여 높게 측정되었으며, 압출성형 산삼배양근의 아미노산 함량은 다소 감소하는 경향을 나타내었다. 유효성분인 조사포닌 함량은 산삼배양근을 압출성형을 통하여 증가시킬 수 있음을 확인하였으며, 배럴온도 120℃에서 압출성형한 산삼배양근이 9.60% 가장 높게 측정되었다. 말톨은 홍삼에서만 확인되었으며, 산삼배양근의 경우 압출성형을 통해서도 말톨의 생성은 나타나지 않았다. 산성다당체는 산삼배양근(0.24 ㎍/g)이 가장 낮았지만 압출성형을 하였을 때 배럴온도 120℃에서 0.75 ㎍/g으로 홍삼의 0.83 ㎍/g과 비슷한 수준까지 증가시킬 수 있었다. 페놀성화합물 함량은 압출성형을 통해 감소하였다. 산삼배양근의 갈색도와 적색도는 압출성형으로 증가하였으며, 배럴온도가 증가함에 따라 증가하였다. 갈색도를 기준으로 한침출특성과 침출속도상수 역시 배럴온도가 증가함에 따라 증가를 확인할 수 있었다. 압출성형은 유효성분인 조사포닌 및 산성다당체 등을 향상시킬 뿐만 아니라 침출력을 향상시켜주므로 향후 고가의 산삼배양근을 더욱 효율적으로 가공할 수 있는 공정으로 확인되었다. The objective of this study is to compare the physicochemical properties and release characteristics of red ginseng (A) and tissue cultured mountain ginseng (B) extruded tissue cultured mountain ginsengs at barrel temperatures 110 (C) and 120℃ (D) to produce tissue cultured mountain ginseng-like comercial red ginseng by extrusion process. Extrusion process variables, water content and screw speed were fixed at 25% and 200 rpm, respectively. In the results, reducing and total sugar content were found to be relatively higher in A. The acidic polysaccharides content of B was the lowest among the ginseng samples. Acidic polysaccharide was increased 3 times by extrusion process. A and B were three times higher at maximum than C and D in polyphenolic compound. Polyphenolic compound content was relatively low by extrusion of ginsengs. Amino acid contents of B, C and D were 35~42 ㎍/mL; in contrast, A contained 25 ㎍/mL. The crude saponin content of C and D were higher than A and B.

속보 운동시간이 제주교잡마의 혈액학 및 혈액화학치에 미치는 효과

양재혁 ( Jaehyuk Yang ),박용수 ( Yong-soo Park ),이수협 ( Suhyup Lee ),정기화 ( Ki-hwa Chung ) 한국축산학회 2021 동물자원연구 Vol.32 No.3

본 연구는 국내 승용마로 가장 많이 이용되는 제주교잡마(14.1±1.4세, 거세)의 운동시간이 혈액학과 혈액화학치에 미치는 영향을 평가하기 위하여 실시하였다. 속보 운동 시간에 따라 바이탈 사인과 스트레스 연관된 cortisol 및 lactic acid를 포함한 총 28가지 항목을 검사하였다. 바이탈 사인 중에서 심박수(평균 38.0→81.0회/분)와 호흡수(평균 11.7→35.7회/분)는 운동 전에 비하여 운동 30분에 유의하게 증가하였다(p<0.05). 그러나 체온은 운동 전후에 차이가 없었다. 혈액학 조사 결과 white blood cell(8.03→9.52×10³cells/μL), red blood cell(5.94×10³→7.23-7.32×10³cells/μL), hemoglobin(11.82→14.65-14.78g/dL) 및 hematocrit(25.04→30.27%) 수치는 운동 전에 비하여 운동 30분에 유의하게 증가하였다(p<0.05). 혈액화학치 조사 결과 알부민(3.25→3.47g/dL)만이 유의하게 증가하였다(p<0.05). 그러나, Na⁺, K⁺, Ca²⁺, total CO₂, creatine kinase, glucose, blood urea nitrogen, creatinine, aspartate transaminase, total bilirubin, gamma-glutamyl transpeptidase 및 total plasma protein 수준은 차이가 없었다. 한편 cortisol 및 lactic acid 수준도 운동 전과 운동 30분에 유의한 차이는 없었다. 이상의 결과를 종합하면, 제주 교잡마에서 30분간 속보 운동은 운동에 의한 스트레스를 받지 않으므로, 승마의 안전성과 동물복지 측면에서 문제가 없을 것으로 판단된다. This study analyzed the effect of time of trot on hematology and blood chemistry values of the Jeju Pony crossbreed horses that are commonly used for riding (14.1±1.4 years old, Gelding). A total of 28 parameters including vital signs as well as stress hormones such as cortisol and lactic acid levels were examined as the time of the trot exercise progressed. Vital signs such as heart rate (38.0→81.0 times/min) and respiratory rate (11.7→35.7 times/min) increased significantly within 30 minutes of exercise. However, difference in the body temperature was not observed before and after exercise. The hematology including white blood cell count (8.03→9.52×10³ cells/μL), red blood cell count (5.94×10³→7.23-7.32×10³ cells/μL), hemoglobin levels (11.82→14.65-14.78 g/dL), and hematocrit levels (25.04→30.27%) significantly increased 30 minutes after the start of the exercise (p<0.05). The blood chemistry value of albumin (3.25→3.47 g/dL) (p<0.05) only showed a significant increase after the exercise. However, the other blood chemistry levels such as, Na⁺, K⁺, Ca²⁺, total CO₂, creatine kinase, glucose, blood urea nitrogen, creatinine, aspartate transaminase, total bilirubin, gamma-glutamyl transpeptidase, and total plasma protein did not change. Also, cortisol and lactic acid levels did not show significant difference. The middle-aged Jeju pony crossbreed horses were not stressed by the 30-minute exercise; therefore, it can be concluded that there is no problem regarding the safety of both the rider and the animal.

차세대 함정용 전기추진처계 기술 개발 현황

류승남(Ryu Seung-nam),전충호(Jeon Choong-ho),정기화(Chung Ki-hwa) 한국마린엔지니어링학회 2009 한국마린엔지니어링학회 학술대회 논문집 Vol.2009 No.-

최근 차세대 선박 및 함정의 추진체계로서 각광받고 있는 전기추진체계에 대한 개념과 차세대 함정에서 고려되는 전기추진체계 관련 기술 개발 동향을 소개하고자 함.

돼지 정자 동결보존에 있어 5 ml straw의 한계성 극복

김범기(Beom-Gi Kim),함형빈(Hyung-Bin Ham),김상현(Sang-Hyeon Kim),손정호(Jung-Ho Son),정기화(Ki-Hwa Chung) 한국생명과학회 2020 생명과학회지 Vol.30 No.1

본 연구는 돼지정자의 동결보존에 있어 0.5 ml straw에 비해 5.0 ml straw가 가지고 있었던 제한적인 요소들을 극복하기 위하여 동결속도, 융해온도 및 융해 시간을 확립하기 위하여 실시되었다. 동결속도는 정자가 -140℃까지 도달하는 시간을 8분 30초인 동결법(FR-1)과 14분인 동결법(FR-2)으로 나누어 각각 0.5 ml 및 5 ml straw를 이용하여 동결하였으며, 동결-융해한 정자는 CASA 장비를 이용하여 정자 성상을 비교 분석하였다. 동결속도 만큼 융해온도 및 시간 또한 매우 중요한 요소이기 때문에 융해 방법을 37℃, 50℃ 및 70℃에서 각각 115초, 45초 및 25초간 실시하여 정자 성상을 비교하였다. 그 결과 FR-2의 동결속도 보다 FR-1의 동결속도에서 높은 생존율과 운동성을 보였고, 50℃에서 45초간 융해 하였을 때 가장 높은 생존율과 운동성을 보였다(73.4±3.6; 74.5±2.2%, p<0.05). 정자의 첨체 충실성 및 형태학적 특징에서는 처리구간에 유의적인 차이가 없었다. 본 연구를 통해 얻은 데이터를 종합해볼 때, 그 동안 동결정액을 이용한 돼지 인공수정 시 5.0 ml straw가 0.5 ml straw 비해 저조한 정자 생존율 및 활력을 나타냄으로써 번식성적이 떨어져 사용을 기피하여 왔지만 개선된 프로토콜을 활용하면 동결정액을 이용한 돼지 인공수정에 있어 여러 straw를 융해해야 하는 불편함 대신에 인공수정 시 1 straw를(dose) 융해하여 사용함으로써 사용자의 편리성을 높일 뿐만 아니라 인공수정에 있어 집중력을 높여 번식성적 향상에도 기여할 것으로 사료된다. The aim of this study was to overcome some of the limiting factors that the maxi cryopreservation straw of 5 ml presents in processing boar semen. Cryopreservation of semen samples was conducted in 0.5 ml and 5.0 ml straws at two freezing rates: -140℃ in 8 minutes and 30 seconds (FR-1) and-140℃ in 14 minutes (FR-2). The straws were then thawed and the semen parameters were compared by Computer Assisted Sperm Analysis, and sperm morphology and acrosome status were examined by Coomassie blue staining. The effects of different thawing temperatures and durations were also compared, namely 37℃ for 115 sec, 50℃ for 45 sec, or 70℃ for 25 sec. In general, the FR-1 group showed higher (p<0.05) sperm viability and motility than the FR-2 group in the 5.0 ml straws. Compared to other ranges, thawing at 50℃ for 45 sec showed the highest sperm viability and motility (68.4±3.6% and 69.5±2.2%, p<0.05), suggesting that thawing temperature should be adjusted concurrently with freezing rate. Sperm morphology and acrosome integrity did not significantly differ among the groups (p>0.05). The data obtained in this study suggest that improving the freezing-thawing protocol for one artificial insemination dose straws (5.0 ml) retains the sperm’s parameters from 0.5 ml cryopreservation, and is more convenient to handle, which could result in enhanced reproductive performance.

한우 공란우 및 생체내 난자 회수(ovum pick-up) 조건이 체외수 정란의 발달에 미치는 효과

박용수 ( Yong Soo Park ),공준호 ( Jun Ho Kong ),이준구 ( Jun Koo Yi ),오동엽 ( Dong Yep Oh ),정기화 ( Ki Hwa Chung ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.4

Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improvement. However, industrialization of embryo production and transfer is necessary to utilize the genetic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 mg GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P<0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and transplantable blastocysts per individual among nine KNC donors (P<0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2∼8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P<0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P<0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2∼4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.