Polymerase chain reaction

전은형 단국대학교 치과대학 예방치과 1998 예방치과 세미나집 Vol.12 No.-

소양호의 질소동화와 광물화

전은형,고유석,한석균,안태영 ( Eun Hyoung Jeon,You Seak Go,Suk Kyun Han,Tae Young Ahn ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.4

From September 1992 to August 1993, nitrogen assimilation and mineralization along with selected biological and physico-chemical environmental factors were investigated 9 times at two different stations in Lake Soyang. For the analysis of assimilation and mineralization, ion concentration analysis method was used. The range of nitrogen assimilation and mineralization rates were 0-100.6㎍-N L^-1 day^-1 and 0∼130.3㎍.-N L^-1 day^-1·respectively. At station 1. where was influent area, assimilation rate which was measured in the surface was correlated positively with temperature (r=0.82. p<0.01) and variation of ammonia (r=0.70, p<0.05) and it was correlated with chlorophyll-a (r=0.87, p<0.01) and nitrate (r= -0.75, p<0.05) in bottom against surface. At station 2, where was Joined with main and influent stream, assimilation rate in surface was correlated with temperature(r=0.77, p<0.05), pH (r=0.70,p<0.05) and ammonia (r=0.69, P<0.05) and mineralization rate in surface was positively correlated with temperature(r=0.86, p<0.01)and pH (r=0.84, p<0.01). Mineralization rate in bottom was correlated with N/P ratio (r=-0.67, p<0.05).

포스터 발표 : 면역 및 세포학 : 혐기성 유류 분해 세균의 분리

전은형,권개경,손재학,이홍금,김상진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1994 한국미생물생명공학회 학술발표초록집 Vol.1994 No.1

Influences of Animal Mucins on Peroxidase Activity in Solution and on the Surface of Hydroxyapatite

이상구,전은형,고홍섭,Lee, Sang-Goo,Jeon, Eun-Hyoung,Kho, Hong-Seop Korean Academy of Orofacial Pain and Oral Medicine 2008 Journal of Oral Medicine and Pain Vol.33 No.3

Animal mucins have structural characteristics similar to human salivary mucins. Animal mucins have been regarded as suitable substances for saliva substitutes. Since animal mucin molecules in saliva substitutes and host-derived antimicrobial salivary molecules exist simultaneously in whole saliva and the pellicles of patients with dry mouth, interactions may occur between these molecules. The purpose of this study was to investigate the influence of animal mucins on peroxidase activity in solution and on the surface of hydroxyapatite(HA) surfaces. The effects of animal mucins on peroxidase activity were examined by incubating porcine gastric mucin(PGM) or bovine submaxillary mucin (BSM) with either bovine lactoperoxidase(bLPO) or saliva samples. For solid-phase assays, immobilized animal mucins or peroxidase on three different HA surfaces(HA beads, HA disc, and bovine tooth) were used. Peroxidase activity was determined with an NbsSCN assay. The obtained results were as follows: 1. PGM enhanced the enzymatic activity of bLPO in solution phase. PGM did not affect the enzymatic activity of peroxidase in saliva sample(POS). 2. BSM did not affect the enzymatic activities of both bLPO and POS in solution phase. 3. HA-adsorbed PGM increased subsequent bLPO adsorption in all three HA phases. The activity of POS was increased on both the HA beads and bovine tooth. 4. The peroxidase activities on the HA beads and disc were increased when the HA surfaces were exposed to a mixture of bLPO and PGM. 5. The binding affinity of bLPO to PGM was greater than that of bLPO to BSM. Collectively, our results suggest that animal mucins affects the enzymatic activity of peroxidase on the HA surfaces as well as in solution. Saliva substitutes containing animal mucins may affect the function of antimicrobial components in natural saliva and saliva substitutes.

삽교호의 세균 다양성과 계통분류학적 분석

김명,전은형,안태영,Kim, Myeong,Jeon, Eun-Hyeong,An, Tae-Yeong 한국미생물학회 2003 미생물학회지 Vol.39 No.4

본 연구에서는 삽교호의 요인 분석과 주변 지류의 영향과 계절에 따른 세균 군집구조의 변화를 분자생태학적 접근 방법을 통해 조사하였다. 시료 채취는 5월과 8월 삽교호 방조제 앞 표층수에서 실시하였으며, 분자생태학적 접근을 위해 시료로부터 DNA를 직접 추출하고, 16S rDNA를 증폭한 후 pGEM-T easy vector에 삽입하여 클로닝을 수행하였다. 획득한 클론 라이브러리를 이용하여 RFLP (restriction fragment length polymorphism)를 분석하였으며, OTUs (operating taxonomy units)로 그룹화하였다. 측정된 종다양성 지수가 8월에 더 높게 나타났으며, 5월의 153개중 34개의 클론과 8월의 131개중 38개의 클론들을 염기서열 분석하였다. 그 결과 Proteobacteria, Cytophaga, Gram positive bacteria와 Verrucomicrobia가 5월과 8월에 공통적으로 분포하는 것으로 나타났으며, 특히 Planctomyces, 시안세균과 엽록체가 조류 대발생이 일어난 8월에 분포하는 것으로 조사되었다. 전반적인 조사결과 삽교호는 전형적인 하구지역의 특성을 나타내었으며 주변 하천으로부터 유입되는 종속영양물질의 영향을 받는 것으로 생각된다. Sapgyo Lake is an artificial freshwater reservoir which is located to the midwest of Korea and is the main water reservoir for industry and agriculture of the region. In this study we investigated environmental factors and the change of bacterial community with the influence of surrounding inflow water and the seasonal variation using the molecular ecological approach. Water samples were collected at front of the dike in May and August, 2001. Bacterial genomic DNAs were extracted directly and purified for the amplification of bacterial 16S rDNA. Clone libraries of the 16S rDNA were constructed using pGEM-T easy vector and RFLP analysis was performed to make a group as OTUs with 4 base recognizing enzymes (MspI and HaeIII). The estimated values of richness in August sample was higher than in May. Thirty-three of 153 clones in May and thirty-eight of 131 clones in August were sequenced from forward region of bacterial 16S rDNA for about 600~800 bp. Proteobacteria, Cytophaga, gram positive bacteria and Verrucomicrobia were observed both months. Especially, Planctomyces, cyanobacteria and chloroplast appeared in August when algal bloom occurred. On the whole investigation, Sapgyo lake showed a typical community structure of estuarine and was influenced by heterochthonous organic matters from the surrounding stream.

Recovery of Genes Epigenetically Altered by the Histone Deacetylase Inhibitor Scriptaid and Demethylating Agent 5-Azacytidine in Human Leukemia Cells

박은경,전은형,김인호,박선양 한국유전체학회 2010 Genomics & informatics Vol.8 No.4

Histone deacetylation and demethylation are epigenetic mechanisms implicated in cancer. Studies regarding the role of modulation of gene expression utilizing the histone deacetylase inhibitor scriptaid and the demethylating agent 5-azacytidine in HL-60 leukemia cells have been limited. We studied the possibility of recovering epigenetically silenced genes by scriptaid and 5-azacytidine in human leukemia cells by DNA microarray analysis. The first group was leukemia cells that were cultured with 5-azacytidine. The second group was cultured with scriptaid. The other group was cultured with both agents. Two hundred seventy newly developed genes were expressed after the combination of 5-azacytidine and scriptaid. Twenty-nine genes were unchanged after the combination treatment of 5-azacytidine and scriptaid. Among the 270 genes, 13 genes were differed significantly from the control. HPGD , CPA3, CEACAM6, LOC653907 , ETS1, RAB37, PMP22, FST, FOXC1, and CCL2 were up-regulated, and IGLL3 , IGLL1, and ASS1 were down-regulated. Eleven genes associated with oncogenesis were found among the differentially expressed genes: ETS1, ASCL2, BTG2, BTG1 , SLAMF6 , CDKN2D, RRAS, RET, GIPC1 , MAGEB, and RGL4. We report the results of our leukemia cell microarray profiles after epigenetic combination therapy with the hope that they are the starting point of selectively targeted epigenetic therapy.

Shewanella putrefaciens DK-1의 Fe(III) 환원 특성

조아영,이일규,전은형,안태영 한국미생물학회 2003 미생물학회지 Vol.39 No.3

Microbial Fe(III) reduction is an important factor for biogeochemical cycle in anaerobic environments, especially sediment of freshwater such as lakes, ponds and rivers. In addition, the Fe(III) reduction serves as a model for potential mechanisms for the oxidation of organic compounds and the reduction of toxic heavy metals, such as chrome or uranium. Shewanella putrefaciens DK-1 was a gram-negative, facultative anaerobic Fe(III) reducer and used ferric ion as a terminal electron acceptor for the oxidation of organic compounds to $CO_{2}$ or other oxidized metabolites. The ability of reducing activity and utilization of various electron acceptors and donors for S. putrefaciens DK-1 were investigated. S. putrefaciens DK-1 was capable of using a wide variety of electron acceptor, including $NO_{3}^{-}$, Fe(III), AQDS, and Mn(IV). However, its ability to utilize electron donors was limited. Lactate and formate were used as electron donors but acetate and toluene were not used. Fe(III) reduction of S. putrefaciens DK-l was inhibited by the presence of either $NO_{3}^{-}$ or $NO_{2}^{-}$. Further S. putrefaciens DK-1 used humic acid as an electron acceptor and humic acid was re-oxidized by nitrate. Environmental samples showing the Fe(III)-reducing activity were used to investigate effects of the limiting factors such as carbon, nitrogen and phosphorus on the Fe(III) reducing bacteria. The highest Fe (III) reducing activity was measured, when lactate as a carbon source and S. putrefaciens DK-1 as an Fe(III) reducer added in untreated sediment samples of Cheon-ho and Dae-ho reservoirs. Shewanella putrefaciens DK-1은 그람음성, 통성 혐기성 세균으로 $NO_{3}$, Fe(III), Mn(IV), humic acid와 같은 다양한 전자수용체를 이용한다. S. putrefaciens DK-1의 전자공여체의 이용능력은 제한적이며, lactate나 formate는 좋은 전자공여체로 이용되지만 acetate나 toluene은 이용하지 못하였다. 다양한 전자수용체간의 경쟁을 살펴보기 위해 전자수용체로 Fe(III)와 같이 $NO_{3}^{-}$, $NO_{2}^{-}$를 넣어 주었을 매 Fe(III)의 환원은 저해되었다. 또한 5. putrefaciens DK-1은 전자수용체로 토양에 광범위하게 존재하는 humic acid를 이용하였으며, 환원된 humic acid는 질산염에 의해서 다시 산화되었다. Fe(III) 환원능이 있는 환경 시료를 이용하여 탄소, 질소, 인과 같은 제한 요인이 Fe(III) 환원세균의 활성에 미치는 효과를 조사하였다. 천호지의 저질토와 대호의 농토에 각각 탄소원, 질소원, 인을 첨가해 주었을 경우 S. putrefaciens DK-1과 탄소원을 동시에 첨가해 주었을 때 가장 높은 철 환원능을 보여주었다.

물리,화학적 방법을 이용한 Cyanobacteria와 식물 플랑크톤의 제어

정원화 ( Weon Hwa Jheong ),전은형 ( Eun Hyung Jeon ),안태영 ( Tea Young Ahn ) 한국환경복원기술학회 2004 한국환경복원기술학회지 Vol.7 No.5

천호지 저질토에서 분리한 철환원세균의 특성

안태영,박재홍,이일규,전은형 한국미생물학회 2002 미생물학회지 Vol.38 No.2

금속 이온 환훤 세균에 의한 철(III)환원은 생물지구화학적 물질순환(biogeochemical cycle)에 무척 중요하다. 이는 크롬(Ⅵ)이나 우라늄(Ⅵ)과 같은 독성 중금속 물질의 환원과 유기물질의 산화에 모텔이 되기 때문이다. 총 37균주의 Fe (III)환원 세균을 소양호와 천호지의 저질토에서 각각 분리하였다. 두 정점 중 초기 Fe (II)의 함유량이 가장 높았던 것은 소양호의 저질토였으나 Fe (III)환훤능은 반대로 가장 낮은 Fe (II)함유량을 보여 주었던 천호지가 높게 나타났다. 또한 분리한 균주 중 천호지에서 분리한 균주 C2와 C3가 가장 높은 Fe (III) 환훤능을 보여 주었으며 이 균주를 이용하여 다양한 전자 공여체의 이용 여부를 실험하였다. Glucose, yeast extract, acetate, ethanol, toluene등을 이용하여 실험한 결과 두 균주 모두 glucose와 yeast extract만을 전자 공여체로 이용하였다. 또한 전자 수용체로 토양에 광범위하게 존재하는 humid acid와 nitrate를 이용하였으며 수율이 높은 nitrate reduction에 의해 환원되었던 humic acid가 다시 재 산화되는 것을 관촬할수 있었다. 활성능이 우수한 균주 C2와C3의 165S rRNA유전자 분석 결과에 의하면 Aeromonas hydrophila와 95%의 유사성을 보여주었다. Microbial Fe (III) reduction is important for the biogeochemical cycle in the sediment of freshwater system. Also, the Fe (III) reducing mechanism make a model of oxidizing organic compounds and reducing toxic heavy metals, such as chrome or uranium. Thirty-seven strains which have Fe (III) reducing activity were isolated from sediments in lake Soyang and Chunho reservoir. The initial concentration of Fe (II) was the highest in sediments of lake Soyang. However, the highest Fe (III) reducing activity was shown in Chunho reservoir. All isolates were tested for Fe (III) reducing activity. Strains C2 and C3, which were isolated from sediments of Chunho reservoir, showed the highest activity. These strains were tested to see if they utilize various electron donors such as glucose, yeast extract, acetate, ethanol and toluene. Significantly, glucose and yeast extract were used as electron donors. Also these strains were conformed to use humid acid and nitrate as electron accepters. The 16S rRNA sequences of strains C2 and C3 were closely related to Aeromonas hydrophila with 95% similarity.

인체 면역 결핍 바이러스 감염 환자의 구내 증상 및 타액 변화

이승우(Sung-Woo Lee),정성창(Sung-Chang Chung),최강원(Kang-Won Choi),김영구(Young-Ku Kim),조군제(Goon-Jae Cho),고명연(Myung-Yun Ko),고홍섭(Hong-Seop Kho),정진우(Jin-Woo Chung),전은형(Eun-Hyoung Jeon) 대한치과의사협회 1996 대한치과의사협회지 Vol.34 No.5