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가축분뇨를 이용한 SCP 생산 균주의 분리 및 균체 단백질 생산
한석균,고유석,안태영,배동훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6
질소원으로서 계분을 이용하는 균주를 선별하고 계분배지에서 균체의 생육속도가 다른 균주에 비하여 우수한 균주를 분리하였다. 형태·생리학적 특성을 기초로 하여 yeast의 분류 기준과 비교하여 본 균주를 Candida sp.로 동정하였으며 본 균주를 Candida sp. D116으로 명명하였다. Poultry feces extract medium에서 4% 농도의 glucose 첨가가 균체 생육에 효과적이었다. D116 균주를 액체 발효하여 균체생산능, 요산 그리고 가용성 단백질의 변화를 조사하였다. 그 결과 약 60시간이 경과하면 액체 발효 배지내의 거의 모든 가용성 단백질 및 요산의 감소를 보였으며 균체생육은 약 36시간 배양하였을 때 최고조에 도달하였고 그 후에는 점차 감소하는 경향을 보였다. SCP의 대량생산의 결과 50%의 계분혼합 배지와 30℃의 배양 온도에서 36시간 배양하여 균주의 생육수준이 3.8×10^9 CFU/ml 농도의 균체를 생산하였고 200 L의 배양액중 약 870 g-dw의 균체를 얻었으며 생산된 군체의 조단백질 함량은 67%이었다. Production of Single Cell Protein from Poultry Feces. Suk-kyun Han, You-Suk Go, Tae-Young Ahn and Dong-Hoon Bal^1*. Deparment of Microbioligy, College of Natural Sciences, Dankook Univerity, Cheonan 330-714 and Research Center for Molecular Microbiology, Seoul Nationa University, Seoul 151-742, Korea, ^1Department of Food Engineering. College of Engineering, Dankook University, Cheonan 330-714 and Bioproducts Research Center of Yonsei University, Seoul 120-749, Korea - From the soil collected form provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete degraded during the microbial growth. Maximum cell growth (3.8×10^9 CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium that in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.
췌장암 환자에서 알코올 내장신경 신경파괴블럭 후 발생한 간염
이상은,최석환,김영환,임세훈,이정한,이근무,정순호,최영균,김영재,신치만 인제대학교 2008 仁濟醫學 Vol.29 No.-
Splanchnic neurolytic block(SNB) with alcohol improves the management of pancreatic cancer pain. Adverse effects of alcohol SNB which include regional pain, hypotension, diarrhea, and acute alcohol intoxication are common, but acute hepatitis caused by alcohol neurolytic block is rare. A 63-year-old patient with pancreatic head cancer and liver metastasis had complete pain relief after both retrocrural tansdiscal SNB with 100% alcohol 10 ml. But, 1 day later, liver function test showed a high elevation in the aspartate aminotransferase (AST, 2182 IU/L) and alanine aminotransferase (ALT, 1807 IU/L). The patient had slight jaundice, general weakness, chilling, nausea and vomiting after SNB. Both AST and ALT decreased to 46 and 119 IU/L within 10 days with only supportive therapy. But, 19 days later, the patient died due to pneumonia. Our case illustrates the importance of clinical surveillance especially in patient with hepatic disease after alcohol SNB.
( Suk Kyun Hong ),( Nam-joon Yi ),( Jeong-moo Lee ),( Kwangpyo Hong ),( Eui Soo Han ),( Kwang-woong Lee ),( Kyung-suk Suh ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Children are not just small adults and the results might be somewhat different from the adult LT. The aim of this study is to evaluate the national cohort of pediatric LT patients and analyze the risk factor for mortality. Methods: Two national registry data were used. One is the Korean Network Organ Sharing (KONOS) of the Korea Centers for Disease Control and Prevention, a mandated national registry started in 2000 but the prospective variables are limited. The other one is the Korean Organ Transplantation Registry (KOTRY), a nationwide organ transplantation registration system, includes many variables but started in 2014. Prospectively collected data of 802 pediatric LT patients between February 2000 and December 2015 from KOTRY and 76 pediatric LT patients between May 2014 and December 2017 were retrospectively reviewed. Results: The 1-, 2-, 5-, and 10-year patient survival rates from KONOS data were 89.5%, 87.5%, 85.7%, and 84.8%. The 1-month, 1-, and 2-year patient survival rates from KOTRY were 92.1%, 89.4%, and 87.2%. There was no significant survival difference between the two registry data. KONOS data of 802 children showed that the mean age was 3.9 years and there were 359 (44.8%) male. Biliary atresia was the leading indication (n=357; 44.5%). KOTRY data of 76 children showed that the mean age was 59.4 months and there were 33 (43.4%). Risk factor for mortality was analyzed using KOTRY data. Hepatic artery complication after liver transplantation was the only risk factor of overall mortality (P<0.001). Conclusions: Long-term pediatric patient survival after liver transplantation is satisfactory.
Suk Kyun Hong,Kwang-Woong Lee,Sola Lee,Su young Hong,Sanggyun Suh,Eui Soo Han,YoungRok Choi,Nam-Joon Yi,Kyung-Suk Suh 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.4
Purpose: The aim of this study was to compare surgical outcomes after liver resection for hepatocellular carcinoma (HCC) according to tumor size using a large, nationwide cancer registry-based cohort and propensity score matching. Methods: From 2008 to 2015, a total of 12,139 patients were diagnosed with liver cancer and registered in the Korean Primary Liver Cancer Registry. Patients without distant metastasis who underwent hepatectomy as a primary treatment were selected. We performed 1:1 propensity score matching between the small (<5 cm), large (≥5 cm and <10 cm), and huge (≥10 cm) groups. Results: Overall, 265 patients in the small and large groups were compared, and 64 patients each in the large and huge groups were compared. The overall and progression-free survival rates were significantly lower in the large group than in the small group (P < 0.001 and P < 0.001, respectively). Overall survival tended to be poorer in the huge group than in the large group (P = 0.051). The progression-free survival rate was significantly lower in the huge group than in the large group (P = 0.002). Conclusion: Although primary liver resection can be considered even in patients with huge HCC, greater caution with careful screening for recurrence is needed.
( Suk Kyun Hong ),( Nam-joon Yi ),( Kwangpyo Hong ),( Eui Soo Han ),( Jeong-moo Lee ),( Kwang-woong Lee ),( Kyung-suk Suh ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Although liver transplantation (LT) has become the standard for treatment of end-stage liver disease in children, there are still some complications that adversely affect the post-transplant outcome. The aim of this study is to identify the risk factors affecting the outcomes in pediatric LT. Methods: Data from pediatric patients who underwent primary LT at Seoul National University Hospital from March 1988 to December 2018, were retrospectively analyzed. Liver disease without “fibro-cirrhosis” was defined as explanted liver showing fibrosis regardless of grade or cirrhosis, or as underlying disease causing progressive liver injury and eventually leading to fibrosis or cirrhosis. Results: There were 255 pediatric LT patients and their 1-, 5-, and 10-year overall survival rates were 90.5%, 88.4%, and 87.8%, respectively and the 1-. 5-, and 10-year graft survival rates were 87.8%, 86.2%, and 84.9%, respectively. Multiple variate analysis identified that liver disease without fibro-cirrhosis as underlying disease (P=0.024) and PELD≥30 (P=0.036) were risk factors of overall survival and body weight <6kg (P=0.028), liver disease without fibro-cirrhosis as underlying disease (P=0.041), and postoperative hepatic artery complication (P<0.001) were risk factors of graft survival. Liver disease without fibro-cirrhosis was the only factor independently associated with hepatic artery complication (P=0.003). Conclusions: More caution is recommended in pediatric LT patients liver disease without fibro-cirrhosis to improve the survival outcome as well as patients with high PELD or low body weight. Hepatic artery complication was the only surgical complications affecting on the graft survival outcome especially in patients having liver disease without fibro-cirrhosis.
Han Sang Park,Jeong-Moo Lee,Kwangpyo Hong,Eui Soo Han,Suk Kyun Hong,YoungRok Choi,Nam-Joon Yi,Kwang-Woong Lee,Kyung-Suk Suh 한국간담췌외과학회 2021 Annals of hepato-biliary-pancreatic surgery Vol.25 No.3
Backgrounds/Aims: From June of 2016, the Model for End-Stage Liver Disease (MELD)-based allocation system replaced the Child- Turcotte-Pugh (CTP) score-based system for organ allocation liver in Korea. The aim of this study was to analyze changes in outcomes and arising issues before and after the implementation of the MELD system. Methods: From June 2014 to June 2018, 129 patients were selected from recipients who underwent deceased donor liver transplantation (DDLT) in Seoul National University Hospital. Pediatric cases were excluded. According to the allocation system, patients were divided into two groups (52 in the MELD group and 77 in the CTP group). Results: MELD scores of the two groups differed significantly (37.8 ± 2.0 in the MELD group vs. 31.0 ± 8.2 in the CTP group; p = 0.001). The etiology of patients was changed for liver transplantation. The proportion of alcoholic liver cirrhosis increased in the era of the MELD allocation system. However, proportions of hepatitis B related liver cirrhosis and hepatocellular carcinoma were decreased. Six-month mortality rate of the MELD group was 25.0%, which was higher than that (11.7%) of the CTP group (p = 0.022). The 90-day complication rate was significantly higher in the MELD group than in the CTP group (11.5% vs. 2.6%; p = 0.040). Conclusions: When the MELD allocation system was used to distribute livers to severely ill patients, it resulted in poorer outcomes after surgery and higher proportion of alcoholic cirrhosis. Thus, it is necessary to adjust the MELD allocation system so that outcomes after DDLT could be improved.