타액 단백질의 기능 및 구조 연구의 임상적 적용

고홍섭 대한안면통증구강내과학회 1998 Journal of Oral Medicine and Pain Vol.23 No.3

Salivary research is at a critical crossroads regarding the clinical application of the basic knowledge. The purpose of this article is to introduce the current progress on salivary research to Korean dental scientists. The accumulated results based on advance technologies such as protein chemistry, molecular biology, and structural biology have showed that salivary macromolecules need structural requirements for proper function. Currently, several concepts or principles, which can be applied to salivary macromolecules, have been suggested. These include the role of molecules' conformation on biological activity, their multifunctional nature, their redundancy of function, their amphifunctional properties, and the potential importance of complexing between molecules. These concepts and the information available will help the development of saliva substitutes, the design of drug carriers and chimera molecules with enhanced function and the development of gene therapy protocols. These approaches will alleviate or restore lost salivary function and can be used to treat various kinds of oral and systemic diseases.

Oral Medicine in Korea, Can We Become a Global Leader?

고홍섭 대한안면통증∙구강내과학회 2015 Journal of Oral Medicine and Pain Vol.40 No.1

editorial

당뇨병환자의 치과시술

고홍섭,Go, Hong-Seop 사단법인 한국당뇨협회 2007 당뇨 Vol.207 No.-

당뇨병환자의 치과시술시 가장 문제가 되는 사항은 감염에 대한 위험성 증가와 창상치유 지연이다. 대부분의 치과치료는 출혈을 일으키고 창상을 남긴다. 만약 치과치료를 예정하고 있는 환자가 당뇨병환자라면 치과의사는 보다 적극적으로 감염 가능성을 예방하거나 줄이고 창상을 최소화하는 방향으로 치과 치료계획을 세울 것이다. 그러므로 당뇨병환자가 치과치료를 받을 때 가장 중요한 사항은 환자 본인의 당뇨 병력을 상세히 치과 진료진에게 알려주는 것이다. 물론 병력은 상세할수록 좋다.

이갈이의 진단과 치료

고홍섭,Kho, Hong-Seop 대한수면의학회 2005 수면·정신생리 Vol.12 No.1

Bruxism is defined as 'diurnal or nocturnal parafunctional activity including clenching, bracing, gnashing, and grinding of the teeth'. Bruxism and clenching are two of the most common contributing factors in patients with temporomandibular disorders and craniofacial pain disorders. Even though many studies report the high prevalence of bruxism, its cause is still not clear. Occlusal interference has been regarded as a major etiologic factor. Nowadays, psychological stress and sleeping disorders are generally regarded as major possible etiologic factors. More than likely, the cause is multifactoral and overlapping, which makes it difficult for the practitioner to apply comprehensive and effective management strategies. Although dentists and psychologists generally believe that effective treatment is best achieved with a better understanding of the etiology of a given disorder, for now treatment for this type of disorder must proceed without a clear understanding of etiology. To overcome this obstacle, evidence-based comprehensive management protocols based on accumulated scientific findings should be provided. In this presentation, epidemiology, etiology, and the characteristics of bruxism are reviewed. Diagnostic procedures and management strategies focused on occlusal appliances and behavioral approaches are also discussed.

Viscosity and Wettability of Hyaluronic Acid according to Antimicrobial Supplementation, Ionic Strength, and pH

고홍섭,장지윤,김윤영,박문수 대한안면통증∙구강내과학회 2014 Journal of Oral Medicine and Pain Vol.39 No.3

Purpose: To investigate viscosity and wettability of hyaluronic acid (HA) solutions according to supplementation of lysozyme and/or peroxidase, and different ionic strength and pH conditions. Methods: Solutions containing HA were prepared using distilled deionized water (DDW) and simulated salivary buffer (SSB) in different conditions. Different concentrations of hen eggwhite lysozyme and bovine lactoperoxidase was added into HA solutions. HA solutions with antimicrobials in different ionic strength and pH conditions were prepared. Viscosity was measured using cone-and-plate digital viscometer at six different shear rates and wettability on acrylic resin and Co-Cr alloy was determined by contact angle. Results: The viscosity values of HA dissolved in DDW were decreased in order of HA, HA containing lysozyme, HA containing peroxidase, and HA containing lysozyme and peroxidase. The viscosity values for HA in DDW were decreased as the concentration of lysozyme and/or peroxidase increased. However, the viscosity values for HA in SSB showed no significant changes according to the concentration of lysozyme and/or peroxidase. The viscosity values of HA solutions were inversely proportional to ionic strength and pH. The contact angle of HA solutions showed no significant differences according to tested surface materials, addition of lysozyme and/or peroxidase, and different ionic strength and pH conditions. Contact angles on acrylic resin by HA solutions in all tested conditions were much higher than those by human saliva. Conclusions: The rheological properties of HA supplemented with lysozyme and/or peroxidase in different ionic strength and pH conditions were objectively confirmed, indicating the possibility of HA with lysozyme and/or peroxidase as main components in the development of effective saliva substitutes.

구강점막 표면 잔존타액 및 Hydroxyapatite 표면 Lysozyme 활성에 관한 연구

고홍섭,이선경,홍정표 대한안면통증∙구강내과학회 2003 Journal of Oral Medicine and Pain Vol.28 No.4

The residual saliva has been known to be important for the maintenance of oral mucosal wetness. Salivary proteins of the oral mucosal film function as a moisture retainer and a protective barrier. Enamel pellicles, derived from saliva, play a significant role in the maintenance and microbial colonization of tooth surfaces. The purpose of this study was to report lysozyme activity of residual saliva and to compare its activity with those of other kinds of saliva, and to investigate the activity of adsorbed lysozyme on the surface of hydroxyapatite. The stimulated and unstimulated whole saliva, stimulated parotid and submandibular-sublingual saliva, and residual saliva on buccal mucosa were collected from a healthy subject. For the investigation of adsorbed lysozyme, hen egg-white lysozyme and unstimulated whole saliva were used as sources of lysozyme. The total protein concentration of all salivary samples was determined using the ninhydrin assay following acid hydrolysis. The lysozyme activity was determined by the turbidimetric method with Micrococcus lysodeikticus. The residual saliva showed higher protein concentration and higher lysozyme activity compared to whole saliva. The residual saliva has more high-molecular-weight proteins compared to other kinds of saliva. The lysozyme displayed decreased activity on the surface of hydroxyapatite. Future additional studies on the composition and activity of both antimicrobial and non-antimicrobial salivary proteins in residual saliva and enamel pellicle are essential.

구강 노쇠는 무엇이며, 왜 중요한가? 구강 노쇠의 정의와 중요성

고홍섭 한국보건행정학회 2022 한국보건행정학회 학술대회 논문집 Vol.2022 No.2

구강점막 상피세포에 부착하는 타액 성분

고홍섭,Kho, Hong-Seop 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.3

The present investigation was carried out to identify salivary components of mucosal pellicle and to explore the difference of mucosal pellicle components according to the location of oral mucosa. By using antisera and immunoblotting, high-(MG1) and low-(MG2) molecular-mass salivary mucins, amylase, IgA, proline-rich proteins(PRPs) were detected in mucosal pellicle in vivo. In addition, the data indicated that mucins, IgA and proline-rich proteins could be cleaved into lower-molecular-mass products, whereas the IgA, proline-rich proteins could also be cross-linked into higher-molecular-mass complexes. Mucosal pellicles from buccal, labial and palatal mucosa showed similar pattern in immunoblotting experiments using anti-MG2 and anti-PRPs antisera. The data from this study suggest that during mucosal pellicle formation multiple components of saliva adsorb to oral mucosal epithelial cell surfaces, and selected components can be proteolytically cleaved into smaller fragments and/or cross-linked into higher-molecular products.

구강 질환 진단용 제제

고홍섭,Kho, Hong-Seop 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.2

The most important progress in diagnostic sciences is the increased sensitivity and specificity in diagnostic procedures due to the development of newer micromethodologies and increasing availability of immunological and molecular biological reagents. The outcome of researches in this field has already provided DNA probes and antibodies which can be used for diagnosing various kinds of diseases including inherited ones. This development can be also applied to diagnose diseases in oral and maxillofacial regions. Technological advances have yielded highly sensitive test methodologies so that low analyte concentration and small sample volume are no longer limiting factors. Therefore, saliva can be useful test fluid for an array of analytes. Salivary constituents of diagnostic significance include steroid hormones, antibodies, drugs, and tumor markers. Of the proteins present in saliva, viral-specific immunoglobulins are of the greatest diagnostic interest. The development of conjugates and antigens by recombinant DNA technique and peptide synthesis is necessary for clinical application. Several kits developed for the purpose of blood testing should be modified to permit their application to saliva. The final practical outcome of researches in diagnostic sciences will be various diagnostic agents which can be used for detection of bacteria and viruses, screening and diagnosis of diseases, genetic screening for forensic individual identification. For these purposes, collaboration researches and development between institutions and companies are essential.

[임상가를 위한 특집 ③] 만성 두개안면통증의 물리치료

고홍섭 대한치과의사협회 2001 대한치과의사협회지 Vol.39 No.4