Anisakis 류에 관한 연구 (I) 각종 해산어에 있어서의 Anisakis류 유충의 분포

전세규,정부관,류봉석,CHUN Seh Kyu,CHUNG Bu Kwan,Ryu Bong Suk 한국수산과학회 1968 한국수산과학회지 Vol.1 No.1

Formerly T. Nishimura, T. Oshima and M. Otsuru studied on Anisakis-like larvae in the various marine fishes from the Japan Sea and the Pacific Ocean. In the present observation, many kinds of marine fishes from the Yellow Sea and the southern coast of Korea were. studied, with the samples from the islands: Eocheng-do, Sohuksan-do, Chuja-do and Keomun-do. The authors examined 313 samples from the adjacent waters of Korea and the distribution of the Anisakis-like larvae in the fish body was found to be 9,219 larvae of Anisakis-like larvae found in the mesentery and coelomic cavity of all samples. Of them, 8,112 Anisakis-like larvae were found from 187 samples from the southern coast of Korea, 1,107 Anisakis-like larvae were found in 126 samples from the Yellow sea (Tables 1 & 2). The fish from the southern coast of Korea are much more infected than the fish from. the Yellow Sea. The larger the fish length, the heavier infection of Anisakis-like larvae were observed.

해양공해와 수족병리

전세규,Chun Seh Kyu 대한수의사회 1977 대한수의사회지 Vol.13 No.6

면역효소항체법에 의한 $\beta$-용혈성 연쇄구균 Streptococcus sp.의 신속진단에 대하여

전세규,최동림,박수일,Chun, Seh-Kyu,Choi, Dong-Lim,Park, Soo-Il 한국어병학회 1988 한국어병학회지 Vol.1 No.2

For the rapid diagnosis of bacterial diseases of cultured fishes, the immunoperoxidase method was applied to the detection of $\beta$-haemolitic Streptococcus sp. strain KST-2 isolated from tilapia(Oreochromis niloticus). The suitability of field analysis and the sensitivity of the immunoperoxidase method was compared with those of the counterimmunoelectrophoresis(CIE) and the immunodiffusion(ID). Results of testing cross-reactivity which did not indicate any cross reactivity with other fish pathogens, this method was specific to Streptococcus sp. The sensitivity of this method was $1{\times}10^3CFU/ml$ which was at least $10^2$times greater than the CIE and $10^4$times greater than the ID. The immunoperoxidase method was more suitable for field application and more sensitive than other diagnostic techniques tested on this study. 양식어류의 세균성 질병의 신속한 진단을 위하여 틸라피아(O. niloticus)로부터 분리된 $\beta$용혈성 연쇄구균인 Streptococcus sp. KST-2균주를 사용하여 면역효소법으로 검출실험을 하였으며, 면역효소법의 현장사용의 용이함과 검출의 민감도를 알아보기 위하여 counterimmunoelectrophoresis(CIE), 및 이중면역확산법등과 비교하여 보았다. 다른 어류 병원균과의 교차반응 결과 교차반응이 전혀 일어나지 않는 것으로 보아 이 방법은 $\beta$용혈성 연쇄구균(KST-2균주)에 매우 특이적임을 알 수 있었다. 연쇄구균의 검출실험한 결과 면역효소법은 $1{\times}10^3CFU/ml$의 농도까지 검출가능하였으며, 검출의 민감성이 CIE에 비해 $10^2$배, 이중면역확산법에 비해 $10^4$배나 높은 것으로 나타났다. 면역효소법은 이번 연구에서 사용된 진단법 중에서 현장사용이 가장 용이하며, 민감성이 가장 뛰어난 것으로 나타났다.

韓國産 쇠우렁 Parafossarulus manchouricus Bourguigant의 形態學的 硏究

田世圭(Seh Kyu Chun) 대한기생충학열대의학회 1964 The Korean Journal of Parasitology Vol.2 No.1

肝吸蟲의 感染經路에 關한 實驗的 硏究

田世圭(Seh Kyu Chun) 대한기생충학열대의학회 1964 The Korean Journal of Parasitology Vol.2 No.3

아질산의 만성중독증에 의한 참장어의 병리조직학적 연구

전세규(Seh Kyu Chun),양한춘(Han Choon Yang) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

The research was carried out to examine the chronic toxic effects of nitrite on the Japanese eel, Anguilla japonica by neans of histological observations. Young eel, 10.8g mean body weight. were exposed to 6 different concentrations of nitrite(1, 5, 10, 20, 30 and 40ppm) for 10 weeks. Each concentration was treated under 5 different levels of pH(5.5, 6.0, 6.5, 7.0 and 7.5) and each of these treatment was tested at 2 different temperature regimes(25℃ and 30℃). Proper concentration of nitrite was made by NaNO₂ and proper pH levels were made by the combinations of 0.1M KH₂PO₄ and 0.1M NaHCO₃. Histopathological test of gill tissues were made along with the test of the formation of thrombocystes and chloride cells on the gill filaments. At the lower pH levels, mucus secretion from the gill was incrased as the nitrite concentration increased. As the level of nitrite increased the number of chloride cells on the gill filament were decreased. Most of the remained chloride cells were observed only at the terminar part of the gill filament at 40ppm of nitrite. Degeneration of gill tissues were observed when nitrite levels were over 10ppm along with detachement and sweption of the epithelial cells of the gill lamellae. Shrunken gill lamellae and formation of thrombosis in the capillaries of gill lamellae were also observed. When temperature goes higher at the higher level of nitrite, necroses in the gill lamellae was increased. At the lower than 10ppm of nitrite, degeneration of gill lamellae was occured at the beginning of the test period but regenerated later. Negative effects of nitrite on the growth of young eel was started between 5∼10ppm at the pH level of 7.0 and 7.5. Thrombosis formation were also started at this level. The safety concentration of nitrite at the pH levels of 7.0 and 7.5 on the small eel seems to be 1ppm. Thrombosis and gill lamella detachment and necrosis in the gill capillaries were not observed at this level. Chloride cells were appeared the whole part of the gill filament.

양식넙치로 부터의 Lysozyme 정제와 어류병원성 세균에 대한 정균작용

전세규(Seh Kyu Chun),박수일(Soo Il Park),김진우(Jin Woo Kim) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

Lysozymes were isolated and purified from various organs of cultured flounder by using chitin-coated cellulose column chromatography. The molecular weights of them were compared with each other in 15% SDS-PAGE gels. The result showed that all lysozymes isolated from various organs of flounder had the same molecular weight of about 14000. To clarify the role of lysoryme as a body defence, the antibacterial activities of flounder lysozyme on seven bacterial pathogens, five Gram-negative and two Gram-positive species, were investigated. The lysozyme had substancial antibacterial activity on four strains, two Gram-negative and two Gram-positive species. These suggest that flounder lysozyme plays a role in body defence against both Gram-negative and Gram-positive bacterial pathogens.

틸라피아에 있어서 Edwardsiella tarda 의 실험적 감염에 관한 연구

전세규(Seh Kyu Chun),최동림(Dong Lim Choi),정준기(Joon Ki Chung),김광희(Kwang Hee Kim) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

Experimental infection method for Edwardsiellosis was studied to imitate histopathologically natural infections in the Tilapia Oreochrimis niloticus. Prior to the bacterial challenge, the intestine of tilapia was damaged by 0.2㎖ of 30% hydrogen peroxide introduced through a silicon tube which was inserted 4 to 5cm into the intestine from anus. E. tarda was mixed a 10% Gum arabia and administered into the stomach by a cannula 20 hours after the hydrogen peroxide treatment. Bacterial doses used were from 4.2 ×10^6 to 6.4 ×10^8 CFU/fish. Fish 72 hours after the challenged showed swollen and erosious lesion and focal necrosis with bacterial-eaden inflammatory cell in the Liver, Spleen, Kidney. Fish 96 and 120 hours after bacterial challenge were moribund showing swollen Liver, Spleen, Kidney and focal accumulation of macrophages and production of granulomas in the infected lesions.

Histopathological study of chronic nitrite toxicity on the japanese eel , Anguilla japonica ; Plate 1 ~ Plate 4

전세규(Seh Kyu Chun),양한춘(Han Choon Yang) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

N/A N/A

능성어, Epinephelus septemfasciatus의 바이러스성 신경괴사증 바이러스의 병원성 연구

손상규,전세규,Sohn, Sang-Gyu,Chun, Seh-Kyu 한국어병학회 1999 한국어병학회지 Vol.12 No.2

The pathogenicity of the fish nodavirus causing viral nervous necrosis (VNN) of sevenband grouper, Epinephelus septemfasciatus was examined in sevenband grouper and other marine fish by intramuscular injection. Sevenband groupers of 27~104 g in body weight were highly susceptible to the fish nodavirus, but yellowtail (537 g in body weight), red seabream (207 g), rock bream (43 g), flounder (41 g), tiger puffer (27 g) and rockfish (94 g) of the sizes used to this experiment were not. The pathogenicity of the viral agent to the sevenband grouper was high without regard to fish sizes at rearing water temperature over $20^{\circ}C$, but not at $15^{\circ}C$. Therefore, susceptibility of sevenband grouper to the viral agent causing VNN was thought to be water temperature-dependent rather than fish size (age-dependent). Compared to the infectivity of the viral agent to sevenband grouper with artificial infection methods, fish were successfully affected by intramuscular, intraperitoneal, oral, dipping and cohabitation administrations although there were slight differences in mortalities among infection methods. And survival sevenband grouper after infection with the fish nodavirus was resistant to the reinfection for a long time. 능성어의 바이러스성 신경괴사증 바이러스(fish nodavirus)의 병원성을 능성어 및 다른 해산어류를 대상으로 인위감염법에 의해 조사한 결과, 능성어 바이러스성 신경괴사증 바이러스는 능성어에서는 소형어(27 g)부터 대형어(104 g)까지 제사를 일으켜 강한 병원성을 나타냈지만, 실험에 사용한 크기의 방어(537 g), 참돔(207 g), 돌돔(43 g), 넙치(41 g), 자주복(27 g) 및 조피볼락(94 g)에는 폐사를 일으키지 않아 병원성을 나타내지 않았다. 그리고 사육수온별 능성어에 대한 바이러스의 병원성은 수온 $15^{\circ}C$에서는 병원성을 나타내지 않았고, 수온 $20\sim28^{\circ}C$에서는 수온이 높을 수록 병원성을 강하게 나타냈다. 그리고 바이러스는 근육주사, 복강 주사 및 경구감염뿐만 아니라 침지감염과 혼합사육감염에 의해서도 쉽게 능성어에 감염이 되지만, 감염 후 생존한 능성어에 대해서는 바이러스를 재감염시켜도 폐사가 일어나지 않았다.