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Drug-Releasing Type Teeth Anti-Aging Coating Agent Using Shellac and Polyvinyl Acetate Composites
임진익 한국고분자학회 2023 폴리머 Vol.47 No.3
Shellac and polyvinyl acetate (PVAc) composites were prepared to protect teeth and obtain a drug release effect. When shellac is used alone, low friction resistance and a easily broken film are formed, but by added polyvinyl acetate and calcium phosphate, the friction resistance is greatly improved, and a stable film state is obtained. The optimal PVAc and calcium phosphate concentration could be obtained through tests using various concentrations. Drug release behavior was tested at neutral and acidic pH through a dye release test, and the death of U-87MG cells was observed in a release test using temozolomide. The composite prepared in this study can be used as a multi-purpose tooth coating agent that can achieve the effect of protecting teeth, coloring specific colors, and releasing drugs.
임진익,김주승,( Le Thi Kim Tram ),김응석,이우걸 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Cyanoacrylates have numerous advantages, including that they can be applied quickly during first aid and can provide good cosmetic outcomes, but they also have limitations in that they have a low bond strength and local tissue toxicity. Consequently, they are primarily used only in urgent applications. To improve both the biocompatibility and the mechanical properties of cyanoacrylate, allyl 2-cyanoacrylate (AC) was prepolymerized and mixed with a dopamine co-initiator. Various properties of prepolymerized AC (PAC)/dopamine mixtures were tested using mouse fibroblast cell (L-929), including their bond strength, setting time, crystallization intensity, and cytotoxicity. Enhanced mechanical properties and biocompatibility were confirmed, and a cytotoxicity test was used to determine the optimal conditions for prepolymerization of AC to be 130°C for 60 min. A combination of 5 mg of dopamine in 5 ml of PAC achieved a high bond strength with cytotoxicity of the dopamine/PAC at approximately 1.5 times lower than that of PAC. These results indicate that dopamine/PAC materials can be extensively used as advanced bio-glues in various applications.
임진익,이우걸,정은진,김지혜 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Protease inhibitors have been usually isolated through a number of steps using various chromatographical methods, which are time consuming and tedious. In this study, an efficient and low-cost polyacrylamide affinity gel electrophoresis (PAAGE) method for the detection and isolation of chymotrypsin inhibitor from a crude extract was studied. The affinity gel was obtained by immobilization of chymotrypsin on 5% (w/v) poly acrylamide-oleic acid gel, and the affinity gel was applied to PAAGE and reverse electrode electro-elution using a modified commercial electrophoresis kit. PAAGE method showed higher isolation efficiency for chymotrypsin inhibitor from Ganoderma lucidum crude extract than a chromatographical method. Specific activity and yield of chymotrypsin inhibitor increased compared with a chromatographical method. Also, two isomers of the inhibitor could be isolated by this method.
Oleic acid-added embedding medium for histological analysis of hard tissue
임진익,김주승,( Le Thi Kim Tram ),김응석,이우걸 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
For the histological analysis of hard tissue such as bone, various acrylatebased materials have been used as an embedding medium. However, commercial embedding media are expensive, and cutting the embedded block takes a long time. In this study, mixtures of methyl methacrylate (MMA), di-butyl-phthalate (DBP), and oleic acid (OA) were tested for possible application as an embedding medium for large and small undecalcified bone specimens. Mechanical properties were tested in a compressive mode. We investigated the change of hydrophilicity in the sectioned surface by measuring the contact angle depending on the OA. Crystallinity was analyzed using a X-ray diffractometer (XRD). Surface analysis was performed using a confocal laser scanning microscope. To determine the staining efficiency of staining dyes, hamatoxylin-eosin (H&E) and Masson’s trichrome (MT) staining methods were performed for the histological analysis of bone-implant complex. We confirmed that the investigated embedding media showed good properties such as optimal mechanical strength appropriate for cutting the embedded block and proper staining efficiency for histological analysis. Therefore, the MMA/DBP/OA mixtures can be used as an embedding media appropriate for various hard tissues and bone-implant complex.
임진익,정은진,성정희,이수희,이우걸 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Allyl 2-cyanoacrylate was pre polymerized and mixed with bis-GMA and hydroxyapatite. Various properties of pre-polymerized AC (PAC)/bis-GMA mixtures were measured, including compressive and shear bond strength, polymerization shrinkage, surface properties, and cytotoxicity; enhanced physical properties and biocompatibility were observed. In particular, the optimal ratio for PAC/bis-GMA/HA was determined to be 90/9/1 wt%, as per the results of the above-mentioned measurements. Polymerization shrinkage of PAC/bis-GMA samples decreased with increasing bis-GMA content (by up to 15 times compared to PAC alone). These results indicate that PAC/bis-GMA/HA materials can be used widely as advanced bioadhesives in various fields. <sup>**</sup>This research was supported by the Ministry of Trade, Industry and Energy(MOTIE), KOREA, through the Education Program for Creative and Industrial Convergence. (Grant Number N0000717)
동결절편을 위해 붕산과 NaOH에 의해 가교된 폴리비닐알코올 포매용 레진
임진익 한국고분자학회 2023 폴리머 Vol.47 No.4
Polyvinyl alcohol (PVA) resin has been generally used as a resin for embedding for cryomicrotomy for rapid histological analysis in the operating room. However, due to the lack of cohesion and penetration of resin, and the relatively high freezing point, and the difference in the mechanical properties of resin and tissue, there have been difficulties in obtaining satisfactory intercepts. In this study, a modified PVA-based embedding resin composed of PVA and boric acid, which can improve histological analysis efficiency, and NaOH as a crosslinking reaction catalyst was studied. It was confirmed that as the amount of PVA, boric acid, and NaOH increased, physical properties including cohesion were improved. And complete tissue cross-section could be obtained without damage using 10 wt% PVA, 2 wt% boric acid, and 0.3 wt% NaOH composition, and hematoxylin and eosin staining tests showed clear tissue images. The modified PVA-containing resin of this study is expected to improve the rapid histological analysis efficiency of various biological tissues.
임진익,김주승,( Le Thi Kim Tram ),김응석,이우걸 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-ε-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14 μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.
Microanalysis using a simple SERS-active substrate based on gold-alumina nanowires
임진익,김주승,정은진,( Le Thi Kim Team ),이수희,이우걸,김지혜,이은경 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Substrate for a surface-enhanced Raman scattering (SERS) was simply fabricated by gold coating on alumina nanowires. A porous alumina film was prepared by anodization of aluminum, and the corresponding gel-like porous film was etched in a diluted NaOH solution to produce densely packed alumina nanowires. The alumina nanowires were then coated with gold using an ion coater. The SERS activity of the nanowires was compared with that of a glass plate, which was used as the control, and the enhanced activity of the gold-coated alumina nanowire substrate was verified at low concentrations of sodium acetate. This study describes an easily prepared and effective substrate for the detection of various molecules.