RT-PCR법을 이용한 백합 바이러스 LSV, LMoV, CMV의 검출

임지현,배은혜,이용진,박성한,이규준,김새로미,정용태,Lim, Ji-Hyun,Bae, Eun-Hye,Lee, Yong-Jin,Park, Sung-Han,Lee, Kyu-Jun,Kim, Sae-Ro-Mi,Jung, Yong-Tae 한국미생물학회 2009 미생물학회지 Vol.45 No.3

2008~2009년에 강원, 충남, 제주 지역의 백합 재배 농가에서 바이러스 감염 증상을 보이는 백합의 잎과 구근을 채취하였으며 RT-PCR 방법으로 Lily mottle virus (LMoV), Lily symptomless virus (LSV), Cucumber mosaic virus (CMV) 등 3 종류의 바이러스를 검출 하였다. LSV 12주, LMoV 20주, CMV 1주가 검출 되었으며 LSV에 감염된 12개의 식물체 중 7개에서는 LMoV도 검출되어 복합 감염된 것을 확인하였다. LMoV와 LSV에 의한 복합 감염은 엽맥투명화, 잎말림, 반점, 모자이크, 황화 줄무늬 등 단독 감염보다 심각한 병징을 나타내었으며 좋지 않은 환경에서 저장된 구근에서도 복합 감염이 관찰되었다. 채집된 식물체는 LMoV 감염이 가장 많았으며 Lily virus X(LVX)에 의한 감염은 검출되지 않았다. 7개 분리주의(LMoV 4주, LSV 2주, CMV 1주) 외피 단백질 유전자를 증폭한 후 염기서열을 분석하여 국내에서 발표된 백합 바이러스 염기서열(LSV:AJ516059, CMV: AJ296154)과 비교 하였으며 LMoV는 국내에서 처음으로 염기서열을 결정하여 기존에 보고된 염기서열(AJ564636)과 비교하였다. 분리주는 기존에 보고된 바이러스와 95~99%의 뉴클레오티드 염기서열 유사성을 보였으며, 이들 분리주의 분자 생물학적 특성을 밝히고 신속하고 정확한 바이러스 진단을 위해서는 전체 염기 서열 분석이 필요한 것으로 판단되었다. Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

물리적 스트레스가 배 ‘신고’의 저장성에 미치는 영향

임지현(Jee Hyun Lim),김진국(Jin Gook Kim),임지혁(Jee Hyuck Lim),임병선(Byung Seon Lim),황용수(Yong Soo Hwang) 한국원예학회 2002 한국원예학회 학술발표요지 Vol.2002 No.6

남성 소비자의 미용행동 연구

임지현 ( Ji-hyun Lim ),서선옥 ( Sun-oak Seo ),김현정 ( Hyun-jung Kim ),박은준 ( Eun-jun Park ) 한국미용예술경영학회 2010 미용예술경영연구 Vol.4 No.3

With diversification of consumer needs and various changes such as the improvement in social status, increase in average life span, and diversification in leisure activities in the service industry, Korean beauty service industry is rapidly growing due to rapid development in the beauty service industry and increasing men consumers. Therefore, this study aims to provide information and academic data to satisfy higher level of men consumers by analyzing men consumers behaviors in the beauty service industry by their demographic characteristics in the beauty service industry and appropriate establishing beauty service strategies. For the study method, self-reported questionnaire survey was used and as to the analysis technique, frequency analysis was used to grasp the study subjects general characteristics and their behaviors when using beauty services. Crosstabs(Chi-Square Test) was conducted to examine users behaviors in beauty service by demographic characteristics. First, the result of how often the subjects use beauty services showed that people in their twenties and thirties use beauty service every six months or bimonthly and people in their forties and fifties use twice a month or every month. The lower the average income gets, the longer the period of using beauty service becomes. Second, when it comes to the favorite beauty salon and the location, people in their 20s and 30s have no favorite beauty salon unlike people in their 40s and 50s. As to the location of the favorite beauty salon, people in their 20s and 30s use the beauty salon near schools while people in their 40s and 50s use the favorite beauty salon near the company and home. As to the average income, those who earn below 2 million won have no their favorite beauty salon while those who earn 2 million to 3 million won and more than 3 million won have their favorite beauty salon. In terms of the location of the favorite beauty salon, those who earn below 2 million won and 2million to 3 million won use near schools while those who earn more than 3 million won use near the company and home. Third, the result of the frequency of visiting favorite beauty salons showed that people in their 20s and 30s use different beauty services between six months and one year while people in their 40s and 50s use beauty services for a long time between 2 years and three years and over three years. The higher the average income gets, the longer the period of using beauty services becomes. Fourth, regarding the average cost of beauty services, people in their 20s and 50s spend 10,000 to 30,000 won while people in their 30s and 40s spend a lot of money on the beauty service. When the monthly average income is higher, the beauty service cost is more spent. Fifth, as to traffic means when using beauty service, most people in their twenties use traffic transportation, people in 30s, 40s, and 50s use their cars or walk to beauty salons. People who earn below 2 million won monthly use public transportation often, people who earn 2 million to 3 million won travel on foot, and people who earn over 300 million won use their cars when using beauty services. According to the above findings, middle-aged men with a stable life invest heavy money in image management and regularly use beauty service industries. The young men are sensitive to fashion and trend and want to often change styles so they have no regular beauty service industries, and furthermore most of them are students and new employees so that they don spend a great deal of money on beauty service industries.

일개 종합병원 종합(민간)검진 비용 영향요인 분석

임지현 ( Ji Hyun Lim ),서원식 ( Won Sik Suh ) 한국병원경영학회 2020 병원경영학회지 Vol.25 No.4

Purpose: In this study, the general characteristics of subjects who spent more than a certain amount of cost for general medical examination at the general hospital health promotion center, and the characteristics of disease, family history, and lifestyle (smoking, alcohol, physical activity, oral care) significantly differed in cost expenditure. We intend to provide basic data for establishing an appropriate marketing strategy for comprehensive examination. Method: It was conducted for users who received comprehensive checkups at a health promotion center at a general hospital in Seoul. The research data collection period is for 979 people who performed comprehensive examinations from January 2019 to December 2020. In order to carry out a comprehensive examination, a questionnaire before the examination was distributed to the subjects who visited the hospital to prepare, and the investigation was conducted in a way that the subjects of the investigation directly filled in. Results: There was a significant influence on the difference in expenditure for comprehensive examination according to the gender, age, and type of health insurance of the subject. In addition, there were significant differences in expenditure according to the presence or absence of disease and the type of family history. Weight loss, smoking history, smoking period, smoking frequency, drinking history, and drinking frequency all had significant effects on cost expenditure. Also, strength training and oral treatment management showed a significant effect on the cost of comprehensive examination. The number of flossing and interdental brushing was also found to have a significant effect. According to the results of multiple regression analysis, disease history (t=2.683, p<.01) and mean smoking frequency (t=4.315, p<.001) appeared to have the most significant effect on expenditure statistically. In other words, when the subject has a history of disease and when the average number of smoking is large, it means that the comprehensive examination cost is remarkably large. Conclusion: By using these contents, hospitals can further refine the marketing of the examination center. In addition, a more convenient and specialized process should be used by patients by linking the general medical department and the examination center well. In terms of management of operating medical institutions, this can be expected to create patients and increase profits.

다윈과 마르크스

林志弦(Lim Jie-hyun) 역사학회 1984 역사학보 Vol.102 No.-

마르크스의 후기사상과 유물사관

林志弦(Lim Jie-hyun) 역사학회 1990 역사학보 Vol.126 No.-

미용소비자 남 · 여 고객에 따른 미용서비스 및 적절한 헤어스타일 시술시간 만족도

임지현 ( Ji-hyun Lim ),정다운 ( Da-un Jung ),김혜정 ( Hye-jung Kim ),박은준 ( Eun-jun Park ) 한국미용예술경영학회 2012 미용예술경영연구 Vol.6 No.3

Beauty care service industry is the industry with high added value, and along with the structural changes of other industries, it is also undergoing constant development. The beauty care market once was centered around women, but with the changes of consciousness on beauty, men now have increased and diversified needs for beauty. This tends to form a new type of beauty care market. Thus, customer satisfaction will lead to the profit creation of beauty care service industry and positive word-of-mouth for reuse. Thereupon, this study divided customer satisfaction into two factors: satisfaction with beauty care services and satisfaction with the hair style treatment time in order to examine the effects of them on customer satisfaction .In order to examine how the beauty care service satisfaction differs by the sex of the beauty care customers, the t-test was conducted. As a result, the male group’s satisfaction was relatively higher than the female group’s. And regarding the treatment time satisfaction by the proper types of treatment according to the sex of the beauty care customers, the male group’s satisfaction was relatively higher than the female group’s. According to the research result, in this rapidly changing society, time is competitiveness to men who lead their lives according to the fixed schedule; thus, not only technical services but more accurate and faster services are required for them.

로드킬(Road-Kill) 방지를 위한 생태통로 모니터링 시스템의 효과에 관한 연구 : 백두대간 구룡령, 백복령을 중심으로

임지현(Lim Jihyun),조혜진(Cho Hyejin),도현구(Do Hyunku) 대한토목학회 2010 대한토목학회 학술대회 Vol.2010 No.10

뮤지컬 보컬 코치의 기능과 역할

임지현(Ji-hyun Lim),민경원(Kyung-won Min) 한국콘텐츠학회 2018 한국콘텐츠학회논문지 Vol.18 No.1

뮤지컬은 제작부터 연출, 작가, 작곡가, 작사가를 비롯하여 안무가, 음악감독, 배우 등 많은 전문가들을 통해 이루어진다. 이처럼 하나의 뮤지컬이 성공하기 위해서는 그 모든 분야의 사람들이 다 함께 창의력을 발휘해야 한다. 라이센스 뮤지컬이 아닌 이상 먼저 작가, 작곡가, 작사가를 통해 한 작품의 기초 틀이 만들어진다. 이들을 크리에이티브 팀(Creative Team)이라 하고 연출가, 안무가, 배우, 스텝 등을 프로덕션 팀 (Production Team)이라고 한다. 그리고 이 두 팀을 합쳐서 크리에이티브 스텝(Creative Staff)이라고 한다. 이후 제작 규모에 따라 2차 크리에이티브 스텝들이 참여하게 되고 실제 연습에 들어가게 되는데 음악감독, 무대 디자이너, 음향 디자이너 등 각 팀의 세부 구성을 이루게 된다. 뮤지컬 크리에이티브 팀에서의 음악 관련 스텝들은 사실 작품의 음악적 색깔과 장르를 결정하는 음악 슈퍼바이저로부터 시작되어 세분화되고 분업화 된다. 하지만 국내에서는 작곡가 또는 음악감독이 그 역할을 모두 담당하고 있다. 본 연구는 해외 뮤지컬 제작 과정 시스템의 사례를 분석하여 작업 공정의 세분화에 따른 보컬 코치의 역할과 개념을 정립하고, 국내 뮤지컬산업에서 보컬 코치의 역할과 필요성을 고찰해 보고자 한다. 일반적인 보이스 티쳐들과 뮤지컬 보컬 코치의 역할과 기능의 공통점과 차이점을 살펴보고, 인터뷰를 통한 국내 뮤지컬 보컬코치 사례를 함께 알아보았다. 그와 더불어 국내 뮤지컬 음악 크리에이티브팀 시스템에 관해 고찰해 보았다. Musical is produced by multiple specialists including director, writer, composer, lyricist, choreographer, music director, actors/actresses and so on. For the success of one musical, all the people with different background should demonstrate their creativities. The basic frame of a work is made by writer, composer and lyricist first unless it is a licensed musical. They are called as Creative Team, and also director, choreographer, actors/actresses and staff are called as Production Team. Both teams are collectively called as Creative Staff. Then, the secondary creative staff members may participate in the work depending on the production size, and each team can consist of the members such as music director, stage designer, sound designer and so on. The staff related to music in Creative Team of the musical is developed with the initiation from the music supervisor who decides the musical color and genre of the work, the fragmentation and the specialization. However, composer or music director takes these roles in charge in Korea. This study aims to establish the roles and concept of vocal coach according to the fragmentation of working process and to investigate their roles and needs in the domestic musical industry upon case analysis of musical production processes in overseas. The common things and differences in the roles and the functions between ordinary voice teachers and musical vocal coaches were analyzed and the cases of vocal coach in the Korean musicals were reviewed by the interviews. In addition, creative team system was reviewed in the Korean musicals.

제7차 세계물포럼 유치활동 경과 및 준비현황

임지현(Ji-Hyun Lim) 대한토목학회 2013 대한토목학회지 Vol.61 No.3