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북방전복, Haliotis discus hannai의 염분 변화에 따른 생리적 반응
신윤경,전제천,임재현,김동욱,손맹현,김응오,Shin, Yun-Kyung,Jun, Je-Cheon,Im, Jae-Hyun,Kim, Dong-Wook,Son, Maeng-Hyun,Kim, Eung-Oh 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.4
염분변화에 따른 북방전복의 생리적 반응을 조사하기 위하여 고염분 및 저염분의 농도에 따라 생존율, 삼투질농도, 대사율 및 조직학적 반응을 분석하였다. 염분변화에 따른 북방전복의 생존율은 37 psu에서는 100%였다. 반면 19.8 psu에서 노출 6일째, 12.8 psu 이하에서는 노출 24시간 이내 모두 사망하여 북방전복의 7-days $LS_{50}$은 24.9 psu (20.1-28.2 psu)를 나타내었다. 염분변화에 따른 호흡률은 대조구에 비해 감소하였으며, 반면 암모니아질소 배설률은 증가하였다. 삼투질농도는 염분 26.8 psu이상에서는 1시간 이내 순치하였다. 북방전복 발근육의 조직학적 반응은 염분감소에 따라 혈림프동의 확장, 상피세포의 괴사 및 식세포 증가 등의 현상이 관찰되었다. This study investigated survival rate, osmorality, respiration, excretion and histological response with change of salinity in the abalone, Haliotis discus hannai at $24{\pm}1^{\circ}C$. Survival rate was 100% at 37.0 psu whereas all died after 6 days at 19.8 psu and within 24 hours at 12.8 psu, respectively. The 7-day median lethal limit (7day-$LS_{50}$) was 20.1-28.2 psu with confidence limits of 20.1-28.2 psu. Respiration rate in low salinity groups was lower than control group, but ammonia excretion rate was more increased in comparison to control group. Osmorality was acclimated within 1 hour at above of 26.8 psu but others could not acclimated at each experimental salinity. Histological observation of foot muscle showed hemolymph sinus distension, epidermal destruction and increased infiltration of phagocytes.
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묵납자루(Acheilognathus signifer) 정자의 냉동보존
정민환 ( Min Hwan Jeong ),민병화 ( Byung Hwa Min ),박미선 ( Mi Seon Park ),명정인 ( Jeong In Myeong ),임재현 ( Je Hyun Im ),임한규 ( Han Kyu Lim ),황형규 ( Hyung Kyu Hwang ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.1
This study aimed to find an optimal diluent and cryoprotective agent (CPA) during cryopreservation of common Korean bitterling Acheilognathus signifier sperm. The bitterling is an endangered species in Korea, and this study will enable conservation and further technical development of artificial seed production. We tested the effects of cryopreservation and toxicity by the type of diluent and CPA. The optimal combination of diluent and CPA for cryopreservation was 300 mM glucose+10% methanol, resulting in a survival rate and sperm activity index (SAI) of 96.3±1.5%, and 3.0±0.0 respectively, and no significant difference compared to fresh sperm. The survival rate and SAI of post-thawed sperm was 9.7±1.5%, 0.8±0.3 respectively, which was significantly higher than had been achieved with other diluents and CPAs.
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