자궁경부암 환자에서의 Lymphoscintigraphy 소견

최창운(Chang Woon Choi),임상무(Sang Moo Lim),홍성운(Sung Woon Hong),박상윤(Sang Yun Park),이제호(Je Ho Lee),이의돈(Eui Don Lee),이경희(Kyung Hee Lee),박기복(Kee Bok Park) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.2

N/A Iliopelvic lymphoscintigraphy was performed to visualize the changes of the lymphatic system using Technetium-99m antimony sulfide colloid in carcinoma of uterine cervix. There were no differences between various indices and clinical stages, but comparison with computed tomographic and postoperative findings, there was meaningful difference in index of decrease and increase, respectively. And lymphoscintigraphy showed by pass way of lymphatic channel in 42 cases (16%) and this suggested the presence of normal shunts of lymphatic channels. Iliopelvic lymphoscintigraphy may be useful for evaluation of lymphatic system in carcinoma of uterine cervix, expecially for screening method.

Taxol 방사면역측정을 위한 125I-Iodotyraminehemisuccinyltaxol ( 125ITHT ) 의 제조

최창운(Chang Woon Choi),임상무(Sang Moo Lim),오옥두(Ok Doo Awh),이태섭(Tae Sup Lee),최태현(Tae Hyun Choi),김현석(Hyun Suk Kim),홍준표(Jun Pyo Hong),이은숙(Eun Sook Lee) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2

목적: Taxol(Paclitaxel)은 난소암과 유방암의 치료제로 사용되고 있으며, 치료시 적절한 체내 혈중농도를 유지함으로서 치료효과를 극대화하기 위해서는 taxol의 혈중농도를 측정할 수 있는 방사면역측정시스템에 표지항원으로 사용할 수 있는 taxol 유도체의 방사성표지화합물을 합성하고, 이를 이용하여 방사면역측정법을 시행할수 있는지의 여부를 확인하고자 하였다. 대상 및 방법: Taxol과 succinic anhydride를 무수 pyridine을 용매로 하여 반응시켜 hemisuccinyltaxol을 합성하고, 합성된 hemisuccinyltaxol과 tyramine을 isobutyl-chloroformate를 coupling agent로 사용하여 tyraminehemisuccinyltaxol을 합성하고 HPLC로 분리정제 하였다. 산화제인 Chloramine-T(5.25 ㎎/ml, 10㎕)를 사용하여 tyraminehemisuccinyltaxol(4 ㎎/ml, 30㎕)에 ^125l(l mCi)를 방사성요오드화하고 HPLC를 이용하여 표지수율을 산정하였다. 정제된 tyraminehenisuccinyltaxol과 ^125l-iodotyraminehemisuccinyltaxol을 80% acetonitrile 수용액에 녹여 4℃와 37℃로 보관하면서, 각 시간대별로 화학적 순도와 방사화학적 순도를 결정하여 그 안정도를 HPLC를 이용하여 확인하였다. [^125l]Iodotyraminehemisuccinyltaxol를 방사성표지항원으로 사용하여 taxol에 대한 단클론항체(3G5A7)의 역가를 검정하였으며, 0∼100nM 농도범위에서 taxol 농도의 증가에 따른 표준투여응답곡선을 작성하였다. 결과: Hemisuccinyltaxol은 79.9%의 수율로 합성되었으며, tyraminehemisuccinyltaxol의 합성수율은 19.5%였다. ^125l-iodotyraminehemisuccinyltaxol의 표지수율은 93%이었다. tyraminehemisuccinyltaxol은 7일까지도 96.5% 이상의 순도를 보여 비교적 안정함을 확인하였으며, ^125l-iodotyraminehemisuccinyltaxol은 3일까지는 93.4% 이상으로 안정하였고 또한 7일 경과시에는 86.1% 이상의 순도를 보였다. taxol에 대한 단클론항체(3G5A7)의 역가를 검정하여 1:256의 역가를 나타냄을 확인하였으며, taxol 농도에 따른 표준투여응답곡선은 taxol과 방사표지 taxol 유도체간에 경쟁적으로 사용되어 직선성(R2=0.971)을 나타내었다. 결론: taxol의 경쟁적 방사면역측정법의 방사성 추적자로서 방사성표지 taxol 유도체인 ^125l-iodotyraminehemisuccinyltaxol을 이용한 방법이 유용함을 확인하였다. Purpose: Taxol(Paclitaxel), an antineoplastic agent, has been used in the treatment of ovarian and breast cancers. The determination of optimal Taxol concentrations in human serum was required for enhancing therapeutic effect and maintaining the appropriate Taxol level in blood. This study was aimed to synthesize radiolabeled Taxol derivatives as radiotracer in competitive radioimmunoassay for monitoring Taxol concentrations in blood and to determine the usefulness of its derivatives. Materials and Methods: Hemisuccinyltaxol(HT) was synthesized by esterification of Taxol with succinic anhydride. Tyraminehemisuccinyltaxol(THT) was synthesized by coupling of HT with tyramine using isobutylchloroformate as coupling agent and purified by HPLC. By using chloramine-T(5.25 ㎎/ml, 10㎕) as oxidant agent, THT(4 ㎎/ml, 30㎕) was labeled with ^125I(37 MBq, 1 mCi). To estimate the stability of purified THT, ^125I-iodotyraminehemisuccinyltaxol(^125ITHT) was dissolved in 80% acetonitrile aqueous solution, and the solution was incubated at 4℃ and 37℃ for 7 days. At various time intervals, the stability of THT and ^125ITHT was monitored. The tiler of Taxol monoclonal antibody, 3G5A7, was determined by competitive radioimmunoassay using ^125ITHT as a labeled antigen. A standard dose-response curve was demonstated by Taxol competitive radioimmunoassay. Results: HT and THT were synthesized with 79.9% and 19.5% yield, respectively. The labeling yield of ^125ITHT was 93%. After 7 days, the chemical purity of THT was 96.5% at 4℃, and 97.5% at 37℃. After 3 days, ^125ITHT was stable with 94.7% at 4℃ and 93.4% at 37℃. After 7 days, radiochermical purity was diminished to 88.1% at 4℃ and 86.1% at 37℃. The titer of Taxol monoclonal antibody, 3G5A7, was determined to 1:256. A standard dose-response curve demonstated good colinearity (R^2=0.971) as Taxol concentration-dependent manner. Conclusion: Competitive radioimmunoassay using ^125I-iodotyraminehemsuccinytaxol as radiotracer could be used to monitor for concentration of Taxol in the human serum.

악성 갈색세포종 및 갑상선수질암의 131I - MIBG 을 이용한 치료

최창운(Chang Woon Choi),임상무(Sang Moo Lim),홍성운(Sung Woon Hong),윤종길(Jong Kil Yoon),류백렬(Baek Yeol Ryoo),이창희(Chang Hee Lee),정상훈(Sang Hoon Jeong),천영국(Young Kug Cheon) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.3

N/A 131I-metaiodobenzylguanidine(MIBG) has been used for the diagnosis and treatment of neural crest tumors. We report our experience with this agent in 8 patients[l multiple endocrine neoplasia(MEN) type IIb; 2 malignant pheochromocytoma; 5 medullary thyroid carcinoma(MTC)]. The therapeutic procedure consisted of 30-200 mCi of 131I-MIBG administered by slow I.V. infusion, given at 3-6 months intervals. Cummulative activity ranged from 150 mCi to 410 mCi, in 1 to 4 courses. 131I-MIBG therapy resulted in significant disease free interval in 1 malignant pheochromocytoma(no measurable lesion)after sugery; complete hormonal and tumoral response in 2 MTC(1 MEN IIb); stable disease in 1 recurred pheochromocytoma(MEN IIb); stable disease but symptomatic improvement in 1 MTC; progressive disease in 1 malignant pheochromocytoma and 2 MTC. The patients who showed progression appeared to have large inoperable tumors or postoperative remnant tumors.

전이성 분화 갑상선암에서 200mCi 방사성 옥소 치료효과 평가를 위한 혈청 Thyroglobulin 추적검사와 전신스캔의 의의

최창운(Chang Woon Choi),임상무(Sang Moo Lim),홍성운(Sung Woon Hong),윤종길(Jong Kil Yoon),이창희(Chang Hee Lee),정상훈(Sang Hoon Jeong),권교선(Gyo Seon Kwun) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.4

N/A Thirty-eight patients with metastatic well-differentiated thyroid carcinoma treated with 200mCi 131I were false negative serum thyroglobulin values during TSH suppression or at anti-thyroglobulin antibody(+) and discrepancies between findings of whole body scan and serum thyroglobulin level. After one to five cycles of 200mCi 131I therapy, complete remission and partial were achieved at 5.3% and 57.9%, respectively. We concluded that all of serum thyroglobulin, TSH, anti-thyroglobulin antibody, 131I or 123I whole body scan were necessary in follow up of metastatic well-differentiated thyroid carcinoma. Also, if there was no response after repetitive 200mCi 131I therapy, higher doses of 131I therapy should be considered.

치료 후 임상적으로 무병상태인 자궁경부암 환자에서 FDG-PET scan의 임상적 유용성에 관한 연구

이재규(Lee Jae Kyu),김법종(Beob Jong Kim),황순혁(Soon Hyunk Hwang),유상영(Sang Young Ryu),김종훈(Jong Hoon Kim),김병기(Byoung Gie Kim),박상윤(Sang Yoon Park),이의돈(Eui Don Lee),이경희(Kyung Hee Lee),최창운(Chang Woon Choi),임상무(Sa 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9

목적: 자궁경부암 환자에서 재발의 조기 진단방법으로 FDG-PET scan의 임상적 유용성을 알아보기 위하여 수술 후 혹은 방사선치료 후 임상적으로 무병상태인 자궁경부암 환자에서 FDG-PET scan을 시행하여 재발의 조기 진단에 있어 FDG-PET scan의 임상적 유용성을 알아보고자 하였다. 연구방법: 자궁경부암으로 원자력 병원에서 수술 후 혹은 방사선치료 후 임상적 무병 상태로 추적 검사 중인 환자 중 전신 FDG-PET scan을 시행한 101명을 대상으로 이학적 검사, 종양표지자, 컴퓨터 단층촬영 등의 기존의 추적 관찰방법과 FDG-PET scan의 영상소견을 비교 분석하였다. 전신 FDG-PET scan의 촬영은 GE advance scanner를 이용하였고, 370-555MBq(10-15mCi)의 FDG를 주사한 후 50분 후부터 촬영을 시작하였으며, 전신 영상을 얻은 후 20분간 국소 영상을 얻었다. 국소 관심 영역의 표준 섭취 계수를 구하여 값이 3.0 이상이거나 주변조직에 비해 높은 섭취율을 보인 경우에 양성으로 판정하였으며 CT, MRI 및 가능한 경우에 경피적 천자술을 시행하여 재발여부를 확인하였다. 결과: PET scan에서 양성소견을 보인 17명(16.8%)중 8명(47.1%)에서 추후 임상적 소견 및 컴퓨터 단층촬영에서 재발이 확인되었으며 이중 2명에서 조직학적으로 재발이 확인되었다. PET scan에서 양성소견을 보인 부위는 종격동 림프절에서 5예, 폐실질에서 5예, 골반림프절에서 4예, 중앙병소에서 3예, 쇄골하 림프절에서 2예로 종격동 림프절과 폐실질에서 가장 많았다. 무병상태로 추적관찰중인 자궁경부암 환자에서 조기재발을 진단하는데 있어 PET scan의 민감도는 100%였으며, 특이도는 90.3%이었다. 양성 예측율(positive predictive value, PPV)은 47.1%(8/17), 음성 예측율(negative predictive value, NPV)은 100%(84/84), 위양성율(false positive rate)은 52.9%(9/17)였다. 결론: FDG-PET scan은 기존의 방사선 검사나 종양 표지자로서 발견하지 못한 전이 또는 재발을 임상적으로 무병상태인 환자의 7.9%(8/101)에서 발견 할 수 있었다. FDG-PET scan은 높은 위양성율에도 불구하고 전신 영상을 함께 얻을 수 있으며 작은병소 혹은 여러 부위에서 전이 또는 재발한 경우 진단이 쉽고 예상치 못한 부위의 원격전이를 찾아낼 수 있어 자궁경부암 환자의 재발의 조기진단에 유용하게 사용될 수 있을 것을 사료된다. Objective: The purpose of this study was to investigate the clinical feasibility of FDG-PET (Positron Emission Tomography) scan in patients with clinically no evidence of disease after treatment of cervical cancer. Methods: One hundred and one patients with clinically NED(no evidence of disease) state after treatment of cervical cancer underwent PET scan. FDG-PET scan was obtained with a GE Advance Scanner, beginning at 50 minutes after injection of 370-555 MBq(10-15 mCi) of 18F FDG. Regional scan was also obtained with emission image. Uptake exceeding 3.0 SUV was determined as a positive finding. Recurrence was confirmed by CT, MRI, and needle biopsy if possible. Results: Among 101 patients showing no evidence of disease, 17 patients(16.8%) showed abnormal PET scan findings. Clinically, 8 patients(7.9%) were confirmed to have recurrent lesion by CT, MRI or by needle biopsy. PET scan could detect recurrent lesions in the mediastinum or lung(10/17), pelvis(7/17), and supraclavicular lymph node(2/17). The sensitivity and specificity of PET scan in patients with cervical cancer showing no evidence of disease were 100% and 90.3%, respectively. The positive predictive value, negative predictive value and false positive rate were 47.1%, 100% and 52.9%. Conclusion: PET scan could detect 7.9% of early recurrence in patients with cervical cancer with NED status. FDG-PET scan may be a useful method in detecting metastases or recurrence of a cervical cancer showing no evidence of disease by routine conventional imaging studies.

신경 베체트병 1례 신경학적 증상과 뇌 PET , SPECT , MRI 소견의 비교

최창운(Chang Woon Choi),김소연(So Yon Kim),조민구(Min Koo Cho),임상무(Sang Moo Lim),이권전(Gwon Jun Lee),김영중(Young Jung Kim),홍성운(Sung Woon Hong),김진욱(Jin Wook Kim),안민(Min An) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.6

We describe a 27-year-old man who developed gait disturbance and dysarthria 2 years after the onset of cardinal symptoms of Behcet's disease. Positron emission tomography with 18F-fluorodeoxyglucose revealed severe hypometabolism in the cerebellum, in accordance wih cerebellar symptoms and sign of the patient. However, single-photon emission tomography with Tc-99m-HMPAO and Tc-99m-ECD did not disclose significant perfusion abnormalities in the brain. Routine brain magnetic resonance imaging did not show signal abnormalities. The findings of imaging studies compared with neurological manifestations of the patient are discussed.

지방육종형성 동물모델에서 123I-15-(p-iodophenyl)-3-R , S-methylpentadecanoic acid ( BMIPP ) 의 생체분포와 생체영상

최창운(Chang Woon Choi),임상무(Sang Moo Lim),이태섭(Tae Sup Lee),서용섭(Yong Sup Suh),우광선,정위섭(Wee Sup Chung),임수정(Soo Jung Lim),오옥두(Ok Doo Awh) 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.5

N/A Purpose: 123I-labeled fatty acids have been used in the evaluation of regional myocardial energy metabolism. This study aimed to evaluate the usefulness of 123I-BMIPP as a liposarcorna-imaging agent. Materials and Methods: We compared in vitro uptakes between liposarcoma(SW872) and glioma(9L) cell lines, and examined biodistribution and in vivo images of 123I-BMIPP in liposarcoma-bearing nude mice. Cold-BMIPP was labeled with 123I using Cu2+ as catalyst. After purification by Sep-pak, radiochemical purity was determined by TLC. We compared cellular uptake between glioma and liposarcoma after incubation of 5, 10, 15, 30, 60, 120, and 180 mins with culture medium containing I-BMIPP. The difference in biodistribution was determined between non-feeding (water only) group for 18 hr and feeding group in normal mice (n=6/group) at 0.5, 2, and 24 hr. In liposarcoma-bearing nude mice model, liposarcoma, SW872, cell lines were injected subcutaneously into the left thigh of nude mice. The biodistribution of 123I-BMIPP was evaluated at 0.5, 2, and 24 hr (n=5 / group) and in vivo image of 123I-BMIPP was obtained with gamma camera at 2 and 24 hr in liposarcorna-bearing nude mice. Results: Radiolabeling yield and radiochemical purity were 95% and above 99%, respectively. SW872 cell line showed more increased uptake than 9L with 1.5 times at 180 mins. The clearance of 'I-BMIPP in various tissues was more delayed in the non-feeding group than in the feeding group, especially at delayed time (24 hr) in normal mice, and the major excreting organ was the gastrointestinal tract. In liposarcoma-bearing nude mice, tumor/blood ratio of 123I-BMIPP was 0.94, 0.75, and 1.38 and tumor/muscle ratio was 0.66, 1.53, and 1.11 at 0.5, 2, and 24hr, respectively. 123I-BMIPP was selectively localized in liposarcoma at 24 hr image. Conclusions: These results suggest that 123I-BMIPP can be used as a liposarcoma-imaging agent. (Korean J Nucl Med 200135:324-333)

단일광자방출단층촬영 영상에서 산란 및 감쇠 보정에 의한 절대방사능 측정

이정림,최창운,임상무,홍성운 ( Jeong Rim Lee,Chang Woon Choi,Sang Moo Lim,Seong Wun Hong ) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4

Purpose: The pvrpose of this study was to evaluate the accuracy of radioactivity quantitation in Tc-99m SPECT by using combined scatter and attenuation correction. Materials and Methods: A cylindrical phantom which simulates tumors (T) and normal tissue (B) was filled with varying activity ratios of Tc-99m. We acquired emission scans of the phantom using a three-headed SPECT system (Trionix, Inc.) witb two energy windows (photopeak window: 126 154 keV and scatter window: 101 123 keV). We performed the scatter correction with dual-energy window subtraction method (k=0.4) and Chang attenuation correction. Three sets of SPECT images were reconstructed using combined scatter and attenuation correction (SC+AC', attenuation correction (AC) and without any correction (NONE). We compared T/B ratio, irnage contrast [(T-B)/(T+B)) and absolute radioactivity with true values. Results: SC+AC images had the highest mean values of T/B ratios. Image contrast was 0.92 in SC+AC, which was close to the true value of 1, and higher than AC (0.77) or NONE (0.80). Errors of true activity by SPECT images ranged from 1 to 11% for SC+AC, 22-47% for AC, and 2 16% for NONE in a phantom which was located 2.4cm from the phantom surface. In a phantom located 10,0cm from the surface, SC+AC underestimated by ?4%, NON.E 40%. However, AC overestimated by 10%. Conclusion: We conclude that accurate SPECT activity quantitation of Tc-99m distribution can be achieved by dual window scatter correc.tion combined with attenuation correction. (Korean J Nucl Med 1998;32:382-90)

Alpha - hCG 측정을 위한 섬광 근접 측정법 ( Scintillation Proximity Assay ) 에 관한 연구

최태현(Tae Hyun Choi),최창운(Chang Woon Choi),임상무(Sang Moo Lim),정위섭(Wee Sup Chung),임수정(Soo Jeong Lim),이수진(Su Jin Lee),이태섭(Tae Sup Lee),오옥두(Ok Doo Awh),(Kwang Sun Woo) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2

목적: 섬광 근접측정법은 항원 항체 반응 후 결합 분획과 유리분획을 분리하는 광정이 필요없다. 이러한 원리를 검체 내 hCG와 항 α hCG 항체간의 항원 항체 반응에 적용하고자 한다. 대상 및 방법: 항 α hCG 항체를 biotin과 결합시켜 SPA bead에 부착된 streptavidin과 부착 가능하게 만들었다. 이 측정법은 항 α hCG 항체가 부착된 SPA beads에 대해 혈청내 hCG와 표지항원인 [^125I]hCG간의 경쟁 반응을 기본 원리로 이용하였다. Biotin표지 항 α hCG항체를 [^125I]hCG 100㎕와 표준용액이나 환자 혈청 200㎕이 들어있는 실온에서 20분 방치하였다. 그리고 streptavidin이 붙은 SPA beads 20㎕를 바이알에 넣고 10분 더 방치한다. 환자 혈청의 수치를 표준 응답곡선을 통해 계산하였다. 결과: SPA측정법에 사용되는 방사성 핵종의 방사능 양에 따라 반응용액 속에서 SPA bead와 자유 방사성 핵종에 의한 배후 방사능이 측정값에 영향이 없음을 확인하였다. SPA 방법을 응용한 측정에서 적합한 표준 응답곡선을 얻었고, 실제 환자혈청에서의 hCG농도를 결정할 수 있었다. 결론: 이 실험을 통해 SPA 방법을 이용한 측정법이 임상진단에 유용하게 사용될 수 있을 것으로 사료된다. Purpose: Scintillation Proximity Assay (SPA) does not require the physical separation of receptor bound form from free form. SPA was applied to the study of interaction of human chorionic gonadotrpin (hCG) and anti-α hCG in serum. Materials and methods: Anti-α hCG was biotinylated for the binding to streptavidin. The assay was based on the simple competitive binding method between [^125l]hCG and the hCG in sample serum, with anti-α hCG-coated beads. Aliquots of biotinylated anti-α hCG were dispensed into scintillation vials containing 100㎕ [^125ㅣ]hCG and 200㎕ of either a standard concentration of hCG for preparation of standard curve or unknown sample, and oncubated for 20 min. at room temperature. Then 20㎕ streptavidin-coated beads were added to vials. and finaliy incubated for 10 min at room temperature. Values for unkncown samples were then calculated from the standard curve. Results: Optimal backfround counts were certificated using varied radioactivity of radionuclides. Appropriate standard curve was obtained from SPA method successively, and the concentration of hCG from unknown serum was determined by standard curve. The result from SPA assay was similar to that of RIA. Conclusion: This observation confims that SPA method could be useful for cilnical diafnosis.(Korean J Nucl Med 2002;36;133-139)

유전자 영상용 HSV1-TK 기질의 합성

안순혁(Soon Hyuk Ahn),최창운(Chang Woon Choi),임상무(Sang Moo Lim),오옥두(Ok Doo Awh),최태현(Tae Hyun Choi) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2

목적: 유전자 치료에서, 종양세포에 herpes simplex virus thymidine kinase 유전자를 발현시키고 이에 민감한 prodrug을 전신투여하여 발현된 종양세포에 특이적으로 집적되는 방법은 매우 효육적인 바업이다. 대표적인 prodrug인, IVDU, IVFRU는 herpes simplex virus type 1의 비침습적 영상화 방법에 응용되는 방사옥소 표지가능 화합물이다. 재료 및 방법: Trans-1-trimethylsilyl-2-tri-n-butylstannylethylene과 uridine 변형체상의 iodo-uracil을 Pd촉매하에서 반응시켜 표지전구체인 5-trimethylvinyl-deoxy-uridine과 5-trimethylsilylvinyl-deoxy-fluororibofuranosyl uracil을 합성하였다. 이것을 column chromatography의 방법으로 분리 정제하였다. 합성한 각 전구체를 ICl 담체를 이용하여 높은 수율로 표지하였고, HPLC를 사용하여 분리하였다. Radiohalogen exchange 방법은 비방사능을 낮게 할수록 표지에 효율적으로 알려져 있다. 이 방법으로 ICl 방법을 사용하여 담체를 포함하는 높은 방사능의 화합물을 얻을 수 있다. 결과: IVDU와 IVFRU 표지를 위한 전구체 합성수율은 각각 43, 72% 였다. ICl로 담체를 이용한 표지방법을 사용하여 IVDU와 IVFRU 표지수율은 98% 이상이었다. 결론: HSV1-tk를 이용한 유전자 영상용 기질의 전구체를 성공적으로 합성하였고, 95% 이상의 높은 표지수율의 유전자 영상용 방사성 추적자를 성공적으로 제조하였다. Purpose: In gene therapy, tumor cells expressing the herpes simplex virus thymidine kinase are sensitive to prodrugs. Potential prodrugs IVDU and IVFRU were synthesized and radiolabeled with radiodine for noninvasive imaging of herpes simplex virus type 1 gene expression. Material and Methods: 5-(2-trimethysilyl)vinyl-2`-deoxyuridine and 5-t(2-trimethylsilyl-2`-fluoro-2`-deoxyuridine, precursors of 5-(2-iodo)viny 1-2`-deoxy uridine(IVDU) and 5-(2-iodo)-2`-vinyl-2`-deoxy-2`-fluororibofuranosyl uracil(IVFRU), were synthesized from reaction of trans-1-trimethylsillyl-2-tri-n-butylstannylethylene with 5-iodo-2`-deoxyuridine and 5-iodo-2`-fluoro-2`-deoxyuridine, respectively, on the condition of Pd catalyst. These precursors were separated from reaction mixture by silica gel column chromatography method. Each precursor was radioiodinated with radioiodine by mixing with ICl oxidizing agent. These radioiodinated compounds were purified with HPKC. Radiohalogen exchange has been shown to be effective for the synthesis of products with lower specific activity. Similarly, carrier-added and high specific activity products have been isolated in respectable radiochemical yields using ICl method. Results: Synthetic yield of precursors, IVDU and IVFRU were 43% and 18%, respectively. Radiochemical purity of both compunds was over 98%. Conclusion: We synthesized precursors of IVDU and IVFRU for monitoring of HSV1-tk gene expression. Radiotracers were radioiodinated with high radiolabeling yield by ICl method. (Korean J Nucl Med 2002;36;102-109)