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임다래 ( Da-rae Lim ),박유리 ( Yu-ri Park ),박선영 ( Sun-young Park ),김혜령 ( Hye-ryung Kim ),박민지 ( Min-ji Park ),구복경 ( Bok-kyung Ku ),나진주 ( Jin-ju Nah ),유소윤 ( So-yoon Ryoo ),위성환 ( Sung-hwan Wee ),전효성 ( Hyo-sung 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.1
In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at 62°C and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was 10<sup>2</sup>, 10<sup>3</sup> and 10<sup>3</sup> TCID<sub>50</sub>/mL for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.
시판 백신접종 스트레스 완화제의 돼지 구제역 백신 접종부위 부작용 발생 저감 효과 평가
박유리,임다래,김혜령,박민지,김범석,김원일,홍창호,김성년,박최규,Park, Yu-Ri,Lim, Da-Rae,Kim, Hye-Ryung,Park, Min-Ji,Kim, Bumseok,Kim, Won-Il,Hong, Chang-Ho,Kim, Sung-Nyon,Park, Choi-Kyu 한국동물위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.3
In this study, we evaluated the efficacy of a commercial vaccine stress-relieving agent (Anti VS-2 injection) to reduce the adverse effects of foot-and-mouth disease (FMD) vaccination site in 120 pigs on two tested pig farms. The results showed that incidence of pig with abnormal meat, size of abnormal meat and weight of discarded abnormal meat are significantly decreased in Anti VS-2 injection-supplemented FMD vaccinated pig groups (n=60) compared to the non-supplemented FMD vaccinated pig groups (n=60) (P<0.05). And the average economic loss by discard of abnormal meat were estimated 99,382 won or 21,514 won in the non-supplemented FMD vaccinated groups or in the Anti VS-2 injection-supplemented FMD vaccinated groups, respectively, showing that the cost saving effect was 78% (77,869 won). In conclusion, it is expected to reduce the incidence and severity of abnormal meat in the FMD vaccinated pigs and to contribute not only to the reduced economic loss of pig farmers but also to the promotion of food hygiene when the Anti VS-2 injection is mixed with FMD vaccine.