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미꾸라지 장기의 (臟器) 단백질과 젖산탈수소효소에 미치는 아연의 (亞鉛) 영향
장미화,정희원,이춘구 ( Mee Wha Jang,Hee Won Chung,Choon Koo Lee ) 한국하천호수학회 1990 생태와 환경 Vol.23 No.3
Zinc accumulation, protein content, electrophoretic pattern of serum protein, LDH activity, and electrophoretic pattern of LDH isozyme in loach after exposure to zinc were studied by biochemical method and electrophoresis. The zinc contents of the normal liver, kidney, and whole blood were 4.86, 9.06, and 6.68 ppm respectively, and those of the liver, kidney, and whole blood were increased to 24.32, 10.70, and 13.44 ppm respectively after loach were exposed to zinc. The total protein contents of the liver, kidney, and serum of control group were 69.00 ㎎/g, 84.80 ㎎/g, and 22.08 g/㎗ respectively, and those of all organs of zinc exposure gorup were decreased to 44.80 ㎎/g, 50.00 ㎎/g, and 19.52 g/㎗ respectively. In the serum protein fractions of treatment group, the absolute values of α₂-globulin, C₃ (β-globulin), and IgG (γ-globulin) were increased, while those of albumin, α₁-globulin, and transferrin (β-globulin) were decreased in comparison to the control. And IgM disappeared after zinc exposure. The total LDH activities of the liver and kidney in zinc exposure group were decreased, while the serum showed the increased level of total LDH activity as compared with the control. In the LDH isozyme percentage, the liver of zinc exposure group showed the decrease of LDH_(1,2,4,5) fractions and increase of LDH₃. In the kidney of exposure group, LDH_(1,2) were decreased and LDH_5 was increased. While, serum of treatment group showed the decrease of LDH₁ and increase of LDH₃, and appearance of LDH_5.
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