생쥐 1-세포기 수정란의 동결방법에 있어서 초자화동결과 완만동결의 비교

이지향,한혁동,구혜영,Lee, Ji-Hyang,Han, Hyuck-Dong,Koo, Hye-Young 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.3

Objective : This study was conducted to examine the effect of vitrification on the survival and in vitro development of mice 1-cell zygotes. Method: Effects of exposure to vitrification solution and vitrification, with different concentrations of the cryoprotectant solution, were examined. The 1-cell zygotes were also subjected to a slow freezing-thawing method to compare with vitrification method. Solution composed of ethylene glycol (6.0 M, 5.0 M, 4.0 M) and sucrose (1.0 M) were used as cryopropectant. The experiments employed the method loading the embryos on electron microscope grids. Results: I. The effects of exposure in vitrification solution. 1-cell zygotes were non-toxic at all concentrations of the vitrification solution showing the survival rate between 88.1% and 97.5%. Development into 2-cell was more successful in the higher concentrations of the vitrification solution. Therefore, higher concentrations of the vitirification solution do not seem to cause any problems in vitrification procedure. II. The effects of vitrification method. 1-cell zygotes showed the survival rate between 78.8% and 92.4%. The lowest and the highest survival rate was observed in the 6.0 M and 4.0 M vitrification solution, respectively. 2-cell development rates varied from 77.6% to 91.3%. Blastocyst development rate was shown highest in 5.0 M and the lowest in 4.0 M solution. Therefore, the highest 2-cell and blastocyst development rate was observed in 5.0 M solution. III. Comparison of vitrification and slow freezing-thawing method on 1-cell zygotes. This experiment showed that 1-cell zygotes had the highest survival and development rates in 5.0 M vitrification solution. Vitrified group of 1-cell zygotes, in the 5.0 M vitrification solution, were compared with the group processed in slow freezing-thawing method. The development rate into 2-cell and blastocyst as well as the survival rate were higher in the vitrified group than in the slowly freezed group. Conclusion: 1. The results demonstrate that the best cryoprotectant is a 5.0 M vitrification solution for 1-cell zygotes. 2. Vitrification method significantly increases the survival rate of the 1-cell zygote and its development into 2-cell and blastocyst. Equilibration and exposure time during the vitrification was remarkerbly short in this experiment. Total time, from the exposure to vitirification solution to storage in the liquid nitrogen, was taken only 90 seconds. In contrast, the slow freezing-thawing method have taken more than four hours. Taken together, we presume that the overall time used for the procedure contributes to the results as an important parameter. 3. The loading of 1-cell zygotes on the EM grid is technically more simple and takes less time than the straw or cryo vial method.

계격산(啓膈散)의 항암(抗癌) 및 면역반응(免疫反應)에 관(關)한 실험적(實驗的) 연구(硏究)

이지향,류봉하,박동원,류기원,Lee, Ji-Hyang,Ryu, Bong-Ha,Park, Dong-Won,Ryu, Gi-Won 대한암한의학회 1997 大韓癌韓醫學會誌 Vol.3 No.1

In order to investigate the effects of Kyegyoksan on antitumor effects after Sarcoma 180 cells transplantation into the peritoneal cavity or left groin in mice, and immune depression in mice induced by methotrexate, the extracts of its herbal medicines were orally administered for 14 or 21 days. Experimental studies were performed for measureance of $IC_{50}$ in MTT assay, mean survival days, tumor and body weights for antitumor effects, delayed type hypersensitivity, hemagglutinine titer, hemolysin titer, rosette forming cells, interleukin-2 productivity, lymphocyte transformation, natural killer cell activity and phagocytic activity for immune responses in the immune depressed ICR mice, and SGOT, SGPT, BUN and creatinine for liver and kidney protective function in SO-rats. The results were obtained as follows: 1. From the results of MTT assay, the Kyegyoksan exstracts for SUN-1 and SUN-C4 were inhibited cell viability. 2. Mean survival time in Kyegyoksan-treated group was slightly increased with no effectiveness, as compared with the control group. 3. Tumor weight in Kyegyoksan-treated group was depressed with the statistical significance, as compared with the control group(p<0.01). 4. Body weight in Kyegyoksan-treated group was incresed with the statistical significance, as compared with the control group(p<0.05). 5. Delayed type hypersensitivity in Kyegyoksan-treated group was slightly incresed with no effctiveness, as compared with the control group. 6. Hemagglutinin titer in Kyegyoksan-treated group was incresed with the statistical significance(p<0.05), but hemolysin titer was slightly incresed with no effectiveness, as compared with the control group. 7. Rosette forming cells in Kyegyoksan-treated group was incresed with the statistical significance, as compared with the control group(p<0.001). 8. Interleukin-2 productivity in Kyegyoksan-treated group was incresed with the statistical significance, as compared with the control group(p<0.001). 9. Lymphocyte transfomation in Kyegyoksan-treated group was incresed with the statistical significance, as compared with the control group(p<0.01). 10. Natural killer cell activity in Kyegyoksan-treated group at E/T ratio 100 : 1 was incresed with the statistical significance(p<0.01), but at E/T ratio 50 : 1 and 10 : 1 was slightly incresed with no effectiveness, as compared with the control group. 11. Phagocytic activity in Kyegyoksan-treated group was slightly incresed with no effectiveness, as compared with the control group. 12. The levels of serum glutamic oxalacetic transaminase, serum glutamic pyruvic transaminase, blood urea nitrogen and serum creatinine in Kyegyoksan-treated group were not effective change, as compared with the control group. According to the above results, it could be suggested that Kyegyoksan have prominent antitumor effects, enhance both cellular and humoral immunity, and have no injury to liver and kidney functions.

율무 약침이 고지방식이 급여 흰쥐의 혈액 내 지질 구성 및 Glucose량에 미치는 영향

이주무 ( Joon Moo Lee ),이상훈 ( Sang Hoon Lee ),이지향 ( Ji Hyang Lee ),이은 ( Eun Lee ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.1

Objective: To investigate the effects of Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture on plasma Lipid composition and glucose concentration in hyperlipidemic rats. Methods: β-lipoprotein, free fatty acids, triglyceride and total cholesterol Levels in Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture groups were compared with those in the control group. Results: Concentration of β-lipoprotein showed no significantly different in all treatment groups. Concentration of free fatty acids, triglyceride and total cholesterol in plasma were decreased in the Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture groups. In the Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture groups, plasma LDL-cholesterol showed a Lower value and HDL-cholesterol showed a higher value than those of the control group. Contents of glucose were decreased in the Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture groups. Conclusions: The results suggest that Yullmoo (Coix Lachryma-jobi var. mayuen stapf.) Pharmacopuncture may have an impact on Lipid metabolism to potentially prevent obesity and enhance treatment effect.

지각(枳殼) 약침이 고지방 식이 급여 흰쥐의 혈액 내 지질구성과 TNF-α 및 IL-6농도에 미치는 영향

이준무 ( Joon Moo Lee ),이종욱 ( Jong Wook Lee ),이지향 ( Ji Hyang Lee ),이온 ( Eun Lee ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.1

Objective: To investigate the effects of Poncirus trifoliata Pharmacopuncture on Lowering Lipid and contents of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in hyperlipidemic rats. Methods: Triglyceride, total cholesterol, TNF-α, and IL-6 Levels in Poncirus trifoliata Pharmacopuncture groups were compared with those in the control group. Results: Concentration of triglyceride and total cholesterol in plasma was decreased in the Poncirus trifoliata Pharmacopuncture groups. In Poncirus trifoliata Pharmacopuncture groups, plasma LDL-cholesterol showed a Lower value and HDL-cholesterol showed a higher value than those of the control group. Contents of TNF-α was decreased in the Poncirus trifoliata Pharmacopuncture groups. Contents of IL-6, however, were not significantly different. Conclusions: The results suggest that Poncirus trifoliata Pharmacopuncture may have an impact on Lipid metabolism to potentially prevent development of diabetes mellitus and accompanying cerebrovascular diseases.

생생한 소식을 전달하기 위한 실시간 인터넷 방송 연구

이지향(Ji-Hyang Lee),주인선(In-Sun Joo),홍두리(Doo-ri Hong),윤용익(Yong-Ik Yoon) 한국정보과학회 2013 한국정보과학회 학술발표논문집 Vol.2013 No.6

인간정보처리 과정에 따른 게임기기 디자인 방향에 대한 연구

이지향(Lee ji-hyang),임창훈(Lim chang-hoon),정도성(Chung Do-Sung) 한국디자인학회 2005 한국디자인학회 학술발표대회 논문집 Vol.2005 No.10

안전약자 상층 대피 지원에 관한 실험적 연구

이지향 ( Ji Hyang Lee ),이효정 ( Hyo Jeong Lee ),권진석 ( Jin Suk Kwon ),박상현 ( Sang Hyun Park ) 한국안전학회(구 한국산업안전학회) 2019 한국안전학회지 Vol.34 No.1

This study is aiming to compare stair ascent transportation speed and physical burden of evacuation supporters according to the types of stair ascent transportation for vulnerable people experimentally. In this study, we measured heart rate of the supporters to indicate physical burden during the transportation. The subjects of this experiment were male students, age of 20-26. Experimental conditions were the ways of stair transportation and the weight of vulnerable people. The types of stair transportation were giving a piggyback ride and carrying a wheelchair. Each experimental trial was video-recorded for measurement of ascent speed and observing supporters movement. As a result of the experiment, as for the ascent transportation speed by piggyback ride from the first floor to the fourth floor, the average speed of the light case is 31 seconds and for the heavy case is 43 seconds. When it comes to the average speed of wheelchair transportation’s average speed the light case is 1 minute and 11 seconds and the heavy case is 1 minute and 49 seconds. Therefore, it was indicated that when the weight of a vulnerable people is lighter, the transportation speed is faster. The heart rates of evacuation supporters are different depending on transportation methods or individual’s condition but as repetitive transportation increases, they tend to reach the maximum heart rates.

전신적 코티코스테로이드 투여 후 발생한 즉시형 과민반응 1예

이지향 ( Ji-hyang Lee ),오혜선 ( Hye-seon Oh ),권병수 ( Byoung Soo Kwon ),박형준 ( Hyngjun Park ),박소영 ( Soyoung Park ),김정현 ( Jung-hyun Kim ),김효정 ( Hyo-jung Kim ),권혁수 ( Hyouk-soo Kwon ),조유숙 ( You Sook Cho ),문희범 ( 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.5

Immediate-type hypersensitivity responses to systemic corticosteroids are rare despite their widespread use. It is still controversial whether the responses rarely occur or are underdiagnosed in clinical settings. Many cases probably remain underdiagnosed because the symptoms often mimic symptoms of underlying diseases. This case report describes a 73-year-old man who had immediate hypersensitivity reactions after intravenous administration of hydrocortisone, which was primarily intended to prevent hypersensitivity reactions to contrast media. Whole body rash with pruritus developed shortly after the steroid injection. Since the patient already experienced rash and itching sense after contrast media and antihistamine injection, we conducted skin testing to figure out which drug triggered the hypersensitivity reactions. Intradermal skin tests revealed a positive response to hydrocortisone sodium succinate, which suggested his hypersensitivity reactions were developed by hydrocortisone. Being a common therapy for allergic reaction, corticosteroids themselves are rarely suspected of causing hypersensitivity reactions. Considering there is no typical symptom or standard diagnostic test, awareness of corticosteroid hypersensitivity reactions is of importance to make the diagnosis. (Allergy Asthma Respir Dis 2016:4:378-381)

경부 경막외 차단을 이용한 연속성 딸꾹질의 치험 3예

이지향(Ji Hyang Lee),김종일(Jeng Il Kim),민병우(By 대한통증학회 1997 The Korean Journal of Pain Vol.10 No.2

Persistent hiccup is defined as duration lasting longer than 48 hours. Reflex arc of hiccup is divided into three parts: afferent, central, efferent. Afferent portion of the neural pathway of hiccup formation is composed of vagus nerve, phrenic nerve, and sympathetic chain arising from T6 to T12. Efferent limb is phrenic nerve. Hiccup center is located in brain stem, midbrain, reticular system and hypothalamus. Persistent hiccup is very difficult to treat by conventional methods. We performed cervical epidural block of the phrenic nerve root for three patients suffering from persistent hiccup. The therapeutic effect was perfect. The mechanism of the cervical epidural block is not yet defined however it is thought to block the efferent nerve fibers and suppress the reflex arc of hiccup. We conclude cervical epidural block is relatively safe and very effective for treating persistent hiccup.

스마트폰 기반 HMD 환경에서 VR 콘텐츠의 유형이 저연령 학습자의 사용자 경험에 미치는 영향

이지향(Ji-Hyang Lee),서민휘(Min-Hwi Seo),계보경(Bo-Kyung Kye),소효정(Hyo-Jeong So) 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2

가상현실(VR) 기술의 발전과 더불어 스마트폰 기반 HMD 를 활용한 가상현실 콘텐츠가 조만간 교육현장에 도입 및 확산될 것으로 예상되고 있으나, 효과적인 교육용 VR 콘텐츠 유형의 본질 및 이에 따른 사용자 경험에 대한 연구는 부족한 것이 현실이다. 따라서 본 연구는 VR 콘텐츠 유형이 저연령(만 12 세미만) 학습자의 사용자 경험에 미치는 영향을 탐색하고자 하였다. 이를 위해 초등학교 사회과 ‘이웃나라’ 단원의 수업을 위해 시각적 구현방식이 다른 사진 기반 VR 콘텐츠와 동영상 기반 VR콘텐츠를 활용하여, 두 가지 다른 콘텐츠 유형이 저연령 사용자의 가상현실 기반 학습 경험에 미치는 영향을 탐색하였다. 초등학생 45 명을 대상으로 실험을 실시하였으며, 연구결과 사진 기반 VR콘텐츠의 용이성 및 만족도가 동영상 기반 VR 콘텐츠보다 유의미하게 높은 것으로 나타났다. 본 연구는 교육용으로 제작되는 VR 콘텐츠의 경우 몰입감 증진을 위한 시각적 구현 방식과 학습자 경험간에 상충(trade-off) 관계가 있을 수 있음을 제시한다.