국민의 식생활과 건강보조식품의 필요성에 관한 연구

이정수 대구산업정보대학 1995 논문집 Vol.9 No.2

As mentioned above,we know that the intake of food by people in our country has nearly approched the encouraged amount of nutrition. Based on this fact, many nutritionists conclude that there is no health impediment due to malnutrition, for the nutritive condition of Korean people today is much better than that in the past,and the intake of food and the encouraged amount of nutrition are nearly balanced. Nevertheless, we should not overlook a few points:imperfection of the encouraged amount of nutrition itself;probable imbalance of nutrition due to excessive intake of manufactured food and fast food;the menace to human health due to theaggravation of environmental pollution ; and probable malnutrition due to the destruction of nutrients during the process of cooking because of imperfect education for nutrition. Therefore, in order to maintain the necessary balance of perfact nutrition, we should duly consider the following points : 1. Elimination of environmental pollution. 2. Consumption of food and drink with natural nutrition(without the process of manufacture and cooking) if possible. 3. Reduction of the intake of manufactureed food as much as possible. 4. Consumption of high fiber food and low carbohydrate food if possible. Putting aside various points of view,we can see that the condition of human health is always threatened by various factor even though the dietary life for human health has nearly approached the encouraged amount of nutrition. Therefore, we should empasize the necessity of preventive measure or improvement of supplemental food for human health.

Verapamil이 심장의 교감신경 β-수용체에 미치는 영향

이정수 ( Jeung Soo Lee ),은종영 ( Jong Young Eun ),조명제 ( Myung Jae Cho ) 한국위생과학회 2004 한국위생과학회지 Vol.10 No.1

개와 쥐 심실의 Muscarinic receptor의 성질에 관한 연구

이정수 ( Jeung Soo Lee ),김명주 ( Myung Joo Kim ) 한국위생과학회 2003 한국위생과학회지 Vol.9 No.1

인삼 Saponin, 은시호 Saponin 및 계면활성제가 적혈구의 용적변화 및 Fragility에 미치는 영향

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),이수근(Soo Kun Lee),옥창건(Chang Kun Ok),김영희(Young Hie Kim) 대한약학회 1989 약학회지 Vol.33 No.1

The effects of Gypsophila saponin, sodium dodecylsulfate (SDS) and Triton X-100 on volume changes and fragility of red blood cells were compared to ginseng saponin to elucidate whether there are any difference in their action on membrane lipid. Cell volume was decreased to about 38% in 1M NaCl and increased to about 20% in 1/10M NaCl. Hematocrit value was decreased by Gypsophila saponin, SDS, and Triton X-100 which caused hemolysis in isotonic NaCl solution. These detergents also inhibited increase of cell volume and accelerated hemolysis in hypotonic solution. However, ginseng saponin did not influence to osmotic volume changes and hemolysis of red blood cells. These results suggest that the disruptive effect of plant saponin on membrane barriers induced by removing membrane lipid is different from their source and ginseng saponin has very low affinity to membrane lipid.

항 히스타민제의 H1 수용체와 무스카린 수용체에 대한 상대적 역가

이신웅(Shin Woong Lee),박영주(Young Joo Park),이정수(Jeung Soo Lee) 대한약학회 1993 약학회지 Vol.37 No.4

The muscarinic antagonist l-[benzilic-4,4''-3H]quinuclidinyl benzilate([3H]QNB) bound to a single class of muscarinic receptor with high affinity in guinea pig ileal membranes. The KD and Bmax values for [3H]QNB calculated from analysis of saturation isotherms were 54pM and 156fmol/mg, respectively. H1-blockers inhibited [3H]QNB binding to ileal membranes with Ki values ranged from 0.008gM to 1.6mcM. The pseudo-Hill coefficients of HI-blockers for inhibition of [3H]QNB binding to the ileal membranes were close to unit. The Ki values for H1-blockers were similar to the Km values calculated by Schild plot of functional data obtained from inhibition of the carbachol-induced contraction in guinea-pig ileum, suggesting that binding of HI-blockers vs [3H]QNB in ileal membranes represents an interaction with a receptor of physiological relevance. The KH values of H1-blockers for H1-receptor estimated from inhibition of the histamine-induced contraction were the range of 0.15nM to 56.5nM. The KM/KH ratio of Hi-blockers varied over a wide range of 3 to 2300. Thus, the antihistaminic potencies of H1-blockers do not correlate with their antimuscarinic potencies, which suggest that antihistamines have different antimuscarinic potencies in therapeutic blood levels causing similar antiallergic effect. Among 13 traditional antihistaminics examined in this study, drug having the highest and the lowest KM/KH ratio is triprolidine and diphenidol, respectively. The present results demonstrate that the antimuscarinic property of antihistamines is not necessary for their antiallergic effect, and data on the affinity of antihistamines for musrinic and H1-receptors can be an important parameter in the selection and evaluation of these drugs.

자궁 무스카린수용체의 확인 및 분류

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),박영주(Young Joo Park) 대한약학회 1992 약학회지 Vol.36 No.3

The muscarinic acetylcholine receptors of the dog unpregnant uterus were characterized using [3H] quinuclidinyl benzilate(QNB) as a radioligand and the binding of muscarinic receptor agonists and antagonists in the uterus was compared to that in the urinary bladder which contains almost exclusively the M2 receptors in order to determine the receptor subtypes in the uterus. [3H]QNB binding to uterus and bladder was rapid, saturable and reversible. Scatchard analysis of the saturation data gave linear plots and the Hill coefficients were close to unit, which indicated that each preparation contained a single population of specific binding sites for [3H]QNB. The KD values(l2OpM) for QNB were almost identical in both organs, whereas the Bmax value of 256fmol/mg protein in the uterus was significantly different from that of 563fmol/mg protein in the bladder. Muscarinic agonists and antagonists inhibited in a competitive manner the [3H]QNB binding to the same extent in both organs. The competition binding studies using antagonists(atropine and pirenzepine) exhibited a single binding site and this site had a low affinity for pirenzepine with the Ki value of about 330nM. However, high and low affinity binding sites were observed with carbachol, methacholine and oxotremorine. These binding studies with agonists and antagonists did not show any differences in drug affinities between uterus and bladder. These results indicate that the muscarinic receptors in the uterus are M2 receptors which have a low affinity for pirenzepine.

개 심실 형질막의 분리 및 그 방향성에 관한 연구

이신웅(Shin Woong Lee),구정옥(Jeoung Ok Ku),이정수(Jeung Soo Lee) 대한약학회 1986 약학회지 Vol.30 No.1

Sarcolemmal membrane fraction from canine ventricle was isolated from the discarded pellet after the first homogenization in the isolation procedure of sarcoplasmic reticulum (Method 1) and the protein yield, purity, and sidedness of this preparation were compared to those of sarcolemmal fraction prepared by method of Lee et al. (Method 2) and a slight modification of original protocol of Jones et al. (Method 3). Method 1 differed from Method 2 essentially only in that vigorous homogenization was carried out by omnimixer and homogenization medium containing 3OmM Tris-maleate was used in the first step. The sarcolemmal fraction was enriched from 45 to 50 and 29-fold in [3H]ouabain, [3H]DHA, [3H]QNB binding and Na+, K+-ATPase activity, respectively, compared to homogenate. Total Na+, K+-ATPase activity of highly sarcolemma enriched fraction was 144.6 +/- 16.4mcmol Pi/mg protein/hr, which was about 85% of total ATPase activity, and the yield of the preparation was 15.7mg protein per 1OOg of starting ventricular tissue. The sarcolemmal preparation supported 45Ca2+-uptake in the presence of ATP but this uptake was not dependent on oxalate. Sarcolemmal Na+, K+-ATPase activity and detectable [3H]ouabain binding were increased about 32% and 35%, respectively, by pretreatment of sarcolemmal fraction with optimal concentration of sodium dodecylsulfate (0.3-0.4mg/mg protein), suggesting that this preparation contained about 24% of sealed rightside-out vesicles, 26% of sealed inside-out vesicles, and 50% of freely permeable (leaky) form. This procedure showed the highest protein yield and leaky population, compared to Method 2 and 3. On the other hand, sarcolemmal fraction prepared by Method 2 and 3 showed low value in protein yield but contained high population of inside-out (46%) and rightside-out (49%) vesicles, respectively, compared to present procedure (Method 1). The results indicate that vigorous homogenization decreases the population of sealed sarcolemmal vesicles but increases the sarcolemmal protein yield per gram tissue and that this procedure is available for further purification of sarcolemmal fraction and for the receptor binding study of sarcolemma.

쥐 심실에서 Digitalis Receptor Desensitization 에 관한 연구

이신웅(Shin Woong Lee),이정수(Jeung Soo Lee),장태수(Tae Soo Jang) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.2

[³H]Ouabain binding parameters (K_D and B_(max)) to control rat ventricular strips and Langendorff preparations which were not previously exposed to ouabain were compared with those to both preparations that had been first exposed to a complete ouabain dose range of dose-response curve (10^(-8) M to 10^(-4) M). In rat ventricular strips and Langendorff perfused heart preparations, cumulative dose-response curves of ouabain revealed biphasic positive inotropic effects, a low-dose effect and a high-dose effect with ED_(50) values of 0.5μM and 35μM ouabain, respectively. The low-dose effect in ventricular strip disappeared or was diminished significantly when the ouabain dose-response curve was repeated after the washout of the effects of the first dose-response curve, whereas there were no significant differences in the maximal high-dose effect in both exposures to oubain. However, both of the control and ouabain-preexposed Langendorff perfused hearts revealed the same low-dose effects. The K_D value for [³H]ouabain binding and the ouabain binding site concentration (B_(max)) estimated by [³H]ouabain displacement assay in control preparations were 230 nM and 2 pmol/mg protein, respectively. [³H]Ouabain binding parameters were not changed by repeated exposure to high concentrations of ouabain. These results suggest that digitalis receptor desensitization in the rat ventricular strip may due to the change of post-receptor events induced by ouabain binding to a high affinity site (α₂ isoform).

칡 열수추출물이 흰쥐의 알코올 대사효소계에 미치는 영향

김명주(Myung-Joo Kim),이정수(Jeung-Soo Lee),하오명(Oh-Myung Ha),장주연(Joo-Yeun Jang),조수열(Soo-Yeul Cho) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1

갈화와 갈근이 알코올성 간손상 흰쥐의 알코올 대사효소계에 미치는 영향을 구명하기 위하여 알코올을 투여한 흰쥐에게 갈화와 갈근 열수추출물을 2수준으로 나누어 5주간 급여한 후 그 효과를 관찰하였다. 체중증가량, 식이섭취량과 식이효율은 알코올 투여시 감소하였으며 갈화와 갈근추출물 급여로 회복되는 경향을 보였다. 장기 무게에 미치는 칡추출물의 영향은 알코올 투여로 증가된 간, 뇌와 심장 무게를 감소시켰는데 갈화가 갈근에 비하여 뇌와 심장 무게의 감소효과가 큰 것으로 나타났다. 알코올 대사효소 활성 변화에서 알코올 투여시 유의적으로 증가된 ADH와 MEOS 활성은 칡추출물 급여시 감소된 반면 AlDH 활성은 대조군에서 유의적으로 감소되었는데 칡추출물 급여시 그 활성이 증가되었다. MAO 활성과 과산화수소 함량도 알코올 투여시 증가되었으며 갈화와 갈근 열수추출물 급여시 유의적으로 감소되었다. 이상의 결과에서 갈화와 갈근 열수추출물 급여는 알코올 중독된 흰쥐의 알코올 대사계 효소 활성 변화에 영향을 미쳐 알코올성 간손상 완화에 효과적일 것으로 사료된다. The effects of Pueraria flos (PF) and Pueraria radix (PR) water extract on the hepatic alcohol metabolic enzyme activities were examined in rats that were orally administered ethanol (25% v/v, 5 g/kg body weight/day) for 5 weeks. The PF and PR water extract were supplemented in a diet, based on 1.2 g or 2.4 g of raw PF or PR/kg body weight/day. Alcohol administration without the PF or PR supplementation significantly decreased net weight gain, feed intake and feed efficiency ratio. However, both dose of the PF or PR supplementation resulted in significant enhancement of growth and suppression of increased relative weight of liver, brain and heart by alcohol administration. Activities of hepatic alcohol dehydrogenase and microsomal ethanol oxidizing system were higher in the alcohol treated group than in the normal group, while aldehyde dehydrogenase activity was significantly lowered in the alcohol treated group. The hepatic metabolic enzyme activities altered by alcohol administration were normalized by both doses of PF or PR supplement. Hepatic monoamine oxidase activity and hydrogen peroxide, which were significantly higher in the alcohol treated group than in the normal group, were also decreased by the supplementation with either PF or PR. These results indicate that low- or high- supplementation of either water extract PF or PR may alleviate ethanol-induced hepatotoxicity by altering alcohol metabolic enzyme acitivities.

인삼 Saponin 이 양신장에서 정제한 Na+, K+-ATPase 의 활성 염산화 및 [3H] Ouabain 결합에 미치는 영향

이신웅(Shin Woong Lee),전갑득(Kap Duck Jin),이정수(Jeung Soo Lee) 영남대학교 자원문제연구소 1986 資源問題硏究 Vol.5 No.-