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      • KCI등재후보

        3차원 운동 입력장치 구현

        이우원,최명환,Lee, Woo-Won,Choi, Myoung-Hwan 강원대학교 산업기술연구소 1999 産業技術硏究 Vol.19 No.-

        In many areas of technology there are machines and systems controllable in up to six degrees of freedom. Helicopters and underwater vehicles, industrial robots are among the first representatives of this category. They need six degrees of freedom in order to move and orient within their workspace. An even broader and more explosively growing area is 3D computer graphics and virtual environment. In this work, functions of 3D input device are described and two types of commercial 3D input device are presented. Then, a preliminary experiment of a low cost 6 axis force/moment sensor is presented that can also be sued as a 3D input device. A low cost force/moment sensor and its application in robot teaching experiment is described. It computes the direction of 3 components of the force and 3 components of the moment applied by human holding the sensor by hand. The concept is shown by an experiment where the tool position and orientation of a robot in 3 dimensional space is controlled by the proposed sensor.

      • KCI등재

        소와 돼지 도체표면에서 황색포도상구균의 분리 및 장독소 검출

        이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),김상현 ( Sang Hyun Kim ),이승미 ( Seung Mi Lee ),이강록 ( Gang Rok Lee ),김금향 ( Geum Hyang Kim ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

        At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 320 pigs and cattle carcass (160 pigs and 160 cattle) slaughtered in Busan province from March 2008 to November 2009. Among 320 samples, 26 of Staphylococcus aureus were isolated from pigs (10.6%) and cattle (5.6%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to oxacillin, cefoxitin, cephalothin, vancomycin, rifampin and linezolid. But the isolates were showed resistance other antibiotics in order of penicillin (92.3%), gentamicin (76.9%), tetracycline (69.2%), erythromycin (65.4%), and clindamycin (61.5%). In case of enterotoxin production, 7.7% of 2 strains produced enterotoxin A.

      • KCI등재

        돼지유래 Salmonella Schwarzengrund의 약제내성 유전자에 관한 연구

        이우원 ( Woo Won Lee ),김상현 ( Sang Hyun Kim ),이승미 ( Seung Mi Lee ),이강록 ( Gang Rok Lee ),이기흔 ( Gi Heun Lee ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.1

        To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (β-lactamase blaTEM), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.

      • KCI등재후보

        Multiplex PCR 기법을 이용한 Salmonella Enteritidis와 S. Typhimurium의 특이적 검출에 관한 연구

        이우원 ( Woo Won Lee ),이승미 ( Seung Mi Lee ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),박호국 ( Ho Kuk Park ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2

        Salmonella species are the most important etiologic agents of food-borne acute gastroenteritis. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the polymerase chain reaction (PCR) has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a multiplex PCR (m-PCR) was used to detect S. Typhimurium and S. Enteritidis. We selected m-PCR target genes, which were the spv (virulence plasmid specific for S. Enteritidis) and sefA (S. Enteritidis fimbrial antigen) genes, fliC (H1-i antigen specific for S. Typhimurium) and a randomly cloned sequence specific for the genus Salmonella. With m-PCR, random sequence was detected from all strains of Salmonella spp, spv and sefA were detected from all strains of S. Enteritidis (100%), and fliC was detected from all strains of S. Typhimurium (100%). This assay indicate that the specificity of the m-PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.

      • KCI등재후보

        소와 돼지유래 Salmonella속 균의 혈청형 및 약제감수성

        이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.1

        At the present study, it was aimed to explore the states of antimicrobial resistant Salmonella spp. isolates from 3,850 pigs (2,732 ileocecocolic lymphnodes and 1,118 cecal contents) and 1,764 cattle (965 cecal lymphnodes and 799 cecal contents) slaughtered in Busan province from December 2000 to November 2001. Among 5,614 samples, 457 of Salmonella spp. were isolated from pig lymphnodes (13.5%), pig cecal contents (4.4%), cattle lymphnodes (3.5%) and cattle cecal contents (0.5%). Salmonella spp. were showed different isolation ratio, that was 10.8% in summer, 9.0% in autumn, 8.4% in spring and 5.0% in winter. As a result of serotyping, B group (65.4%) were identified as the most common in pigs and cattle, in order of C1 (14.0%), D1 (5.5%), C2 (4.2%), E1 (4.2%) and L (3.5%). 34 serotypes were found, among them, Salmonella Typhimurium (S. Typhimurium) (21.0%) was the most common serotype from pigs and cattle. The major serotypes were in order of S derby (15.3%), S. Schwarzengrund (14.7%), S. Typhimurium var Copenhagen (9.2%), S. Mbandaka (5.7%), S. Enteritidis (5.5%) and S. Ruiru (3.5%). The most common serotype was S. Typhimurium in pigs, and S. Ruiru in cattle. S. Ruiru was firstly isolated from pigs and cattle in Korea. In antimicrobial susceptibility test, all the isolates were demonstrated susceptibility to norfloxacin and ofloxacin. But the isolates were showed resistance other antibiotics in order of doxycycline (68.3%), tetracycline (67.8%), penicillin (54.5%) and streptomycin (52.5%). S. Typhimurium were exhibited resistance to ampicillin (34.8%), chloramphenicol (36.2%), streptomycin (94.9%), sulfamethoxazole/trimethoprim (34.8%) and tetracycline (97.8%). There were 53 strains (38.4%) which had multidrug resistant (MDR) isolates, resistant to more than 6 antimicrobial agents. The most common resistance patterns of MDR isolates were ampicillin, chloramphenicol, carbenicillin, doxycycline, nalidixic acid, penicillin, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACCbDNaPSSuT).

      • KCI등재후보

        소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성

        이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.1

        At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. definitive type 103 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. chwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.

      • KCI등재후보

        소와 돼지유래 살모넬라속균의 약제내성유전자의 특성에 관한 연구

        이우원 ( Woo Won Lee ),정병열 ( Byeong Yeal Jung ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3

        At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM (blaTEM) gene was detected from 51 (92.7%) of 55 β-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 β-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sul II (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

      • KCI등재

        호흡기증세 자돈으로부터 파스튜렐라 속균분리 및 약제감수성시험

        이우원 ( Woo Won Lee ),우병길 ( Byung Gil Woo ),김홍태 ( Hong Tae Kim ),이강록 ( Gang Rok Lee ),이동수 ( Dong Soo Lee ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.3

        This study were carried out to investigate isolation of Pasteurella multocida from respiratory disorder piglets, to examine the biochemical properties and antimicrobial susceptibility. The results were summarized as follows; P multocida was isolated from 31(10.3%) of the 302 respiratory disorder or growing piglets of 4~10 week olds. The majority of biochemical and cultural properties of the P multocida isolates were identical to those of the standard strains. The isolates were highly susceptible to norfloxacin(100.0%), enrofloxacin(96.8%) and ampicillin(87.1%), but resistant to streptomycin (77.4%), penicillin(61.2%) and neomycin(54.8%).

      • DSP를 이용한 리니어 모터의 토크 제어기 개발과 힘제어 응용

        이우원(Woo Won Lee),임계영(Kye Young Lim),황상연(Sang Yeon Hwang) 전력전자학회 2006 전력전자학술대회 논문집 Vol.- No.-

        일반적으로 리니어 구동기나 회전하는 모터를 이용하여 적은 힘으로 중량의 물체를 이송하는 시스템이 개발되어 현자에서 사용되고 있다. 그러나 공기압이나 유얍을 사용하는 리니어 구동기나, 회전하는 모터를 사용하기 때문에 Backlash를 비롯한 비선형성이 크기 때문에 성능의 한계를 갖는다. 본 논문은 동기형 리니어 모터를 구동하는 DSP 기반의 힘 제어기를 설계, 제작하고 힘제어를 이용하여 이송하는 시스템을 개발하고 시험한 내용을 보여준다.

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