A review of the genus Callipterinella van der Goot (Hemiptera: Aphididae: Calaphidinae) in Korea

이예림,Seung Hwan Lee 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.3

The aphid genus, Callipterinella van der Goot, 1913 (Calaphidinae: Calaphidini: Calaphidina) was reviewed from Korea. Previously recorded species, Callipterinella calliptera (Hartig, 1841), and hitherto unrecorded species, Callipterinella tuberculata (von Heyden, 1837), were recognized in Korea. A key to species, species description, photographs of live aphids, and illustrations are provided for the two species.

항진균활성 violacein 색소를 생산하는 Collimonas sp. DEC-B5 균주의 분리 및 특성

이예림,황경숙,Lee, Ye-Rim,Mitchell, Robert J.,Whang, Kyung-Sook 한국미생물학회 2016 미생물학회지 Vol.52 No.2

Forty-nine pigments were extracted from the collections of 106 pigment producing bacteria from the plant rhizosphere soil. Antibacterial activity test was performed in the subjects of the extracted pigments with plant pathogenic bacteria including Xanthomonas axonopodis and Xanthomonas campestris, and with plant pathogenic fungi including Botrytis cinerea, Colletotrichum acutatum, and Fusarium oxysporum. The yellow pigment by Chryseobacterium sp. RBR9 and the red pigment by of Methylobacterium sp. RI13 showed the antibacterial activities against Xanthomonas axonopodis and Xanthomonas campestris. The violet pigment by Collimonas sp. DEC-B5 showed the antibacterial activity as well as the antifungal activities against Botrytis cinerea and Fusarium oxysporum. Especially, the violet pigment inhibited the growth of Botrytis cinerea more than 65% at MIC $20{\mu}M$. Upon the HPLC analysis result for the isolation of pigment with antifungal activity, violacein (91.6%) and deoxyviolacein (8.4%) were isolated for the pigment by Collimonas sp. DEC-B5. The production amount of the pigment was increased more than 10 times higher when D-mannitol 1.5% and yeast extract 0.2% were added as the nitrogen source to SCB medium. This study suggests that produced violacein by Collimonas sp. DEC-B5 will be effective to control strawberry gray-mold rot fungi by its preventive activity. 식물 근권 토양으로부터 색소생성 균주 106균주를 수집하여 색소 생성능이 우수한 균주로부터 노란색(33개), 주황색(12개), 분홍색, 빨간색, 갈색 그리고 보라색 총 49개의 세균색소를 추출하였다. 식물병원균에 대한 항균활성능이 우수한 색소를 선발하기 위하여 고추점무늬병원균(Xanthomonas axonopodis), 흑마병원균(Xanthomonas campestris)과 딸기잿빛곰팡이병원균(Botrytis cinerea), 고추탄저병원균(Colletotrichum acutatum), 그리고 시들음병원균(Fusarium oxysporum)을 대상으로 항균활성 검정을 수행하였다. 색소생성 Chryseobacterium sp. RBR9 균주가 생산하는 노란색 색소와 Methylobacterium sp. RI13 균주가 생산하는 빨간색 색소는 X. axonopodis와 X. campestris에 항세균 활성을 나타내었다. 차나무 토양으로부터 분리된 Collimonas sp. DEC-B5가 생산하는 보라색 색소는 항세균 활성과 더불어 B. cinerea와 Colletotrichum acutatum에 항진균활성을 나타내었다. 특히, 보라색 색소는 최소저해 농도 $20{\mu}M$에서 B. cinerea를 65% 이상 생육 저해하였다. 항진균활성 보라색 색소를 HPLC 분석한 결과, violacein (91.6%)와 deoxyviolacein(8.4%)으로 동정되었다. 보라색 색소 violacein의 생산량은 SCB 배지에서 $43.2{\mu}M$이었고 D-mannitol 1.5%, yeast extract 0.2%를 첨가한 경우 $431.6{\mu}M$로 약 10배 높은 색소 생성량을 나타내었다. 본 연구에서 분리된 Collimonas sp. DEC-B5가 생산하는 violacein 색소는 딸기잿빛곰팡이병원균 방제제로 활용 가능성이 확인되었다.

First Record of the Genus Kaochiaoja Tao, 1963 (Hemiptera: Aphididae) in Korea

이예림,이승환,Lee, Yerim,Lee, Seunghwan Korean Society of Applied Entomology 2018 한국응용곤충학회지 Vol.57 No.1

본 연구에서는 Kaochiaoja arthraxonis (Takahashi, 1921) 종을 기반으로 Kaochiaoja Tao, 1963 속을 국내 최초로 보고한다. 무시형 처녀생식세대의 암컷 사진 형태 정보를 제시하였다. The genus Kaochiaoja Tao, 1963 is newly recognized in Korea based on the type species Kaochiaoja arthraxonis (Takahashi, 1921). Diagnosis and illustrations for apterous viviparous females are given.

First Record of the Subfamily Neophyllaphidinae Takahashi, 1921 (Hemiptera: Aphididae) in Korea

이예림,이승환,Lee, Yerim,Lee, Seunghwan Korean Society of Applied Entomology 2018 한국응용곤충학회지 Vol.57 No.1

본 연구에서는 최근 확인된 Neophyllaphis podocarpi Takahashi, 1920를 기반으로 Neophyllaphidinae Takahashi, 1921 아과를 국내 최초로 보고한다. 무시형과 유시형 처녀생식세대의 암컷 사진 및 유시형 산란모, 수컷 형태 정보를 수록하였다. The subfamily Neophyllaphidinae Takahashi, 1921 is newly recognized in Korea based on the recently collected species Neophyllaphis podocarpi Takahashi, 1920. Diagnosis and illustrations for alate and apterous viviparous females, alate oviparous female and male are provided.

First Record of the Genus Tinocallis Matsumura (Hemiptera: Aphididae) on Lagerstromia speciosa in Laos

이예림,이승환,Lee, Yerim,Lee, Seunghwan Korean Society of Applied Entomology 2016 한국응용곤충학회지 Vol.55 No.2

Tinocallis Matsumura 속은 전세계 19종이 포함되는 비교적 큰 속이다. 본 연구에서는 최근 라오스에서 바나바 (부채꽃과: 배롱나무속)에 심각한 피해를 주는 알락진딧물 일종, Tinocallis himalayensis Ghosh, Ghosh & Raychaudhuri, 1971를 처음으로 발견하여 보고한다. 유시형 처녀생식세대의 암컷 형태, 생태 및 피해 사진을 함께 제시하였다. The genus Tinocallis Matsumura, 1919 is one of the large genera of the subfamily Calaphidinae with 19 species worldwide. From recent expeditions in Laos, we recognized the heavy infestation of Tinocallis himalayensis Ghosh, Ghosh & Raychaudhuri, 1971, which is new record in Laos, on Queen's crape-myrtle, Lagerstroemia speciosa (Lythraceae). In this paper, the diagnosis and illustrations for the alate viviparous female are provided with the images of live aphids and damage on host plant.

경기지역 한국인 대학생과 중국인 유학생의 배달음식 이용형태 및 만족도 비교 연구

이예림,장재선,Lee, Ye-Rim,Jang, Jae-Seon 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.4

This study was conducted to understand the type of use and satisfaction of delivery food by Chinese and Korean students. In December 2019, Korean and Chinese students living in Gyeonggi Province were analyzed using SPSS. The average number of food deliveries was 1~2 times a month and used 30.7% of the Korean students and 21.8% of the Chinese students. As for how to order food for delivery, 62.2% of Korean college students took up mobile apps, and 49.0% of Chinese students took up mobile phones. In terms of satisfaction after using delivery food, Korean students scored 3.89 points for taste and 3.74 points for using a neat and convenient packaging container, while Chinese students showed the highest score of 3.69 points for past eating experiences and 3.65 points for taste. As for the factors influencing the satisfaction after using the delivered food, the food quality satisfaction (β=0.518) for Korean students and favorable menu factor (β=0.533) for Chinese students were found. Therefore, it is judged that it is necessary to continuously improve the quality, service, convenience and diversity of delivered food, and health and safety factors in order to improve the delivery patterns and satisfaction of college students.

형질전환 벼에서 brazzein 감미단백질의 안정적인 발현

이예림,샤히나,이인혜,정여진,박소영,조용구,강권규,정유진,Lee, Ye Rim,Akter, Shahina,Lee, In Hye,Jung, Yeo Jin,Park, So Young,Cho, Yong-Gu,Kang, Kwon Kyoo,Jung, Yu Jin 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.1

Brazzein은 열대식물인 P. brazzeana Baillon의 과실에서 분리된 가장 작은 감미단백질로 토착민들의 단맛원료로 사용되어 왔다. Brazzein은 sucrose보다 분자량 기준으로 500 ~ 2000배, 몰 기준으로 9500배 당도가 높아 감미료로써 매우 높은 평가를 받고 있다. 그러나 이 감미단백질은 재배가 어렵고 생산 비용이 높아서 brazzein 단백질의 이용 가능성을 높이기 위한 대체 생산 시스템으로 형질전환 식물체 육성 하고자 하였다. 본 연구에서는 brazzein 관련 유전자를 벼에 도입하기 위하여 식물형질전환용 Ti-plasmid에 $2{\times}CaMV\;35S$ 프로모터에 의해 지배되어 발현하도록 하고, 선발 마커로 bar 유전자가 삽입된 식물발현 벡터를 구축하여 A. tumefaciens EHA105에 형질전환시켜 17개의 재분화 식물체를 육성하였다. 17개 재분화 식물체는 PCR 및 RT-PCR 분석을 통하여 유전자 도입 및 발현을 확인하였으며, TaqMan PCR을 통해 single copy로 도입된 T0 세대 9개체를 선발하였다. 또한 FST 분석을 통하여 도입 유전자가 intergenic으로 삽입된 개체 5개를 선발하였다. 이들 5개체를 이용하여 western blot 분석에 의해 단백질 발현량을 분석한 결과 선발된 모든 개체에서 발현 밴드를 확인하였다. 그 중 brazzein 단백질의 발현량이 높은 개체를 TG11으로 계통화하여 후대 종자를 육성하였다. TG11 계통은 천연 감미료 brazzein을 생산하는 새로운 벼 품종을 개발하기 위한 육종 소재로 활용 가능하다고 시사된다. Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener.

Gd₃Ga₅O₁₂ 기판위에 성장된 Y₃Fe₅O₁₂ 박막의 열처리 조건에 따른 강자성 공명 특성 연구

이예림,박승영,정종율,Lee, Yelim,Phuoc, Cao Van,Park, Seung-Young,Jeong, Jong-Ryul 한국재료학회 2015 한국재료학회지 Vol.25 No.12

In this study, we investigated the effect of annealing conditions on the ferromagnetic resonance(FMR) of yttrium iron garnet ($Y_3Fe_5O_{12}$, YIG) thin film prepared on gadolinium gallium garnet ($Gd_3Ga_5O_{12}$, GGG) substrate. The YIG thin films were grown by rf magnetron sputtering at room temperature and were annealed at various temperatures from 700 to $1000^{\circ}C$. FMR characteristics of the YIG thin films were investigated with a coplanar waveguide FMR measurement system in a frequency range from 5 to 20 GHz. X-ray diffraction(XRD) and X-ray photoelectron spectroscopy(XPS) were used to characterize the phase formation, crystal structure and composition of the YIG thin films. Field dependent magnetization curves at room temperature were obtained by using a vibrating sample magnetometer(VSM). The FMR measurements revealed that the resonance magnetic field was highly dependent on the annealing condition: the lowest FMR linewidth can be observed for the $800^{\circ}C$ annealed sample, which agrees with the VSM results. We also found that the Fe and O composition changes during the annealing process play important roles in the observed magnetic properties.

Microstructure and Mechanical Properties on Friction Stir Welded AA2195

Ye-Rim Lee(이예림),Ho-Sung Lee(이호성),Sang-Hoon Lee(이상훈),Sang-Woo Song(송상우) 대한용접·접합학회 2015 대한용접학회 특별강연 및 학술발표대회 개요집 Vol.2015 No.11

인공신경망 기반 비등각 온도제어 블로우 성형 공정예측 모델 개발

이지웅(J. W. Lee),강병권(B. K. Kang),이예림(Y. L. Lee),정영찬(Y. C. Jeong),홍석관(S. K. Hong),이창호(C. H. Lee),이상원(S. W. Lee) 한국생산제조학회 2021 한국생산제조시스템학회 학술발표대회 논문집 Vol.2021 No.12