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RISS 인기검색어
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- 저자 : 이수택(Soo Taeck Lee) (검색결과 1 건)
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Retinoic Acid가 Bromodeoxyuridine 표지 PLC / PRF / 5 간암 세포주의 역동성에 미치는 효과
안득수(Deuk Soo Ahn),김대곤(Dae Ghon Kim),송석현(Suck Hyun Song),김이엽(Ee Yup Kim),장동석(Dong Suck Jang),이수택(Soo Taeck Lee) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.6
N/A Retinoie acid (RA) has been reported to show antiproliferative and differentiation-inducing effects on human tumor cells. In this report, we examined the antiproliferative effect and the influence on the cell cycle in PLC/PRF/5 hepatocelluar carcinoma cells, secreting hepatitis B surface antigen (HBs Ag). MTT assay was conducted to prove the antiproliferative effect of RA, and the univariate and the bivariate distribution of BrdU/DNA were analyzed using FACScan, to evaluate the influence of RA on tumor cell kinetics in the labelled cells with the 5-bromodeoxyuridine (BrdU). Following 6 days of exposure, 1uM and 0 1 uM RA containing nontoxic DMSO level showed antiproliferative effect on the cells. The analysis from the tumor cell kinetics showed that actual doubling time (Td) is 69 hrs, potential doubling time (Tpot) is 42 hrs, the mean DNA synthesis time (T5) is 17.4 hrs, and the labelling index is 35.2%. The changes of the tumor cell kinetics in the cells treated with RA were investigated. Early stage of culture (day 2, 4) showed increase in S phase percentage of cells and decrease in G0/G1 phase. But ]ate stage of culture (day 6) show decrease in S phase percentage of cells to minimum and increase in G0/G1 phase at either 1 uM or 0.1 uM concentration of RA as compared with that of control. The influence on cell cyclc was rnore pronounced at 1 uM than at 0.luM concentration. These results demonstrated that RA inhibits the growth of PLC/PRF/5 cells and its antiproliferative effect is suggested to be driven from arresting cell cycle progression from G0/G1 to S phase in tumor cell kinetics.
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