초자화 동결된 생쥐 완전탈출 배반포기배 이식에 관한 유용성 검토

이봉경,김은영,남화경,이금실,윤산현,박세필,임진호 한국동물번식학회 1998 Reproductive & developmental biology Vol.22 No.2

This study was carried out to confirm whether the in vivo developmental potential of mouse hatched blastocysts (HBs) can be obtained by vitrification method using the cryoprotectant EFS35. The HBs ($\theta$ 130$\mu\textrm{m}$) were cultured in vitro until day 5 from zygotes produced in vivo and were equilibrated in 10% ethylene glycol(EG) for 5 min, and then were exposed or vitrified in EFS35 (35% EG, 18% Ficoll and 0.3M sucrose). After 30 min thawing, re-expanding HBs were transferred into one or both uterine horns of pseudopregnant recipients on day 3 (4~6 embryos /horn). Pregnancy rates of recipients and implantation were assessed by autopsy on day 15 of gestation. The results obtained in these experiments were summarized as follows : After thaw-ing, in vitro survival of HBs was not significantly different between exposed (65.5%) and vitrified(54.5%) group. Also, when the in vivo development potential was examined, total implantation was not different between control (58.5% ) and vitrified (41.0%) group, although the live fetus formation of vitrified group (24.0%) was significantly lower than that of control (58.3%) group (p< 0.05). These results suggested that vitrification freezing method of mouse HBs using EFS35 can be used to make wide the utility of embryo transfer of the more embryos produced in vitro.

The Cytoskeletal and Chromosomal Constitution in Vitrified Mouse Oocyte

이봉경,김은영,남화경,이금실,윤산현,박세필,임진호,Yi, B.K.,Kim, E.Y.,Nam, H.K.,Lee, K.S.,Yoon, S.H.,Park, S.P.,Lim, J.H. The Korean Society for Reproductive Medicine 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

본 연구는 동해방지제인 EFS35를 이용한 생쥐 미수정란의 초자화동결이 cytoskeleton과 염색체에 미치는 영향을 indirect immunocytochemistry와 염색체 분석으로 확인하고자 실시하였다. 생쥐 미수정란은 M2 배양액에 35% ethylene glycol, 18% ficoll, 0.3 M sucrose와 10% FBS가 함유된 EFS35를 이용하여 초자화동결되었다. 본 연구에서 얻어진 결과는 다음과 같다 초자화동결된 미수정란의 융해후 생존율은 89.3%로써 노출군 (97.7%)과 차이가 없었다. 초자화동결이 미수정란의 microtubule과 microfilament에 미치는 영향을 조사하였던 바, 동결군 (95.5, 100%)의 microtubule과 microfilament의 정상적인 형성율은 대조군 (97.5, 100%) 또는 노출군 (92.3, 100%)의 결과와 비교하였을때 차이가 나타나지 않았다. 또한, 체외수정 후 동결 군에서 정상적인 염색체수를 가진 난자의 비율도 73.5%로써, 대조군 (79.5%)과 노출군 (78.7%)의 결과와 차이가 없었던 바, 생쥐 미수정란을 EFS35에 노출하고 동결하는 것이 미수정란의 cytoskeleton과 염색체 성상변화에 영향을 미치지 않는다는 것을 알 수 있었다. 따라서, 본 연구에서 사용된 동해방지제인 EFS35를 이응한 초자화동결법은 생쥐 미수정란의 동결에 적합하다는 것을 시사한다고 하겠다. The objective of this study was to confirm whether the vitrification method using EFS35 has detrimental effect for cytoskeleton and chromosome constitution of the mouse oocytes by indirect immunocytochemistry and chromosome analysis. Mouse oocytes were vitrified using EFS35 which consisted of 35% ethylene glycol, 18% ficoll, 0.3 M sucrose and 10% FBS in M2 medium. The results obtained in this experiment were summarized as follows: When the survival rates after being exposed or vitrified in EFS35 were examined, there were not different between two groups (97.7 and 89.3%). Also, when the microtubule morphology and microfilament distribution in vitrified oocytes were examined, normal percentage of two cytoskeleton in vitrified group (95.5 and 100%) was not different from that in control (97.5 and 100%) and exposed group (92.3 and 100%). In addition, the rate of oocytes containing a normal chromosome number in vitrified group (73.5%) after IVF was not different from that in control (79.5%) and exposed group (78.7%). These results indicated that the cytoskeletal morphology and chromosome constitution of mouse oocytes were not affected by cryoprotectant (EFS35) or freezing apd that vitrification methods using EFS35 was suitable for cryopreservation of mouse oocytes.

Receptor-mediated Transport of Vitellogenin during Oogenesis of a Polychaete, Pseudopotamilla occelata

이봉경,남중현,이양림,Lee, Bong-Gyeong,Nam, Jung-Hyeon,Lee, Yang-Rim The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.2

Receptor-mediated endocytosis has been suggested for a stage-specific transport mechanism of vitellogenin into the oocytes of a sabellid poly chaete, Pseudopotamilla occelata. Membrane proteins of oocytes of three size classes, including small (30-70 $\mu\textrm{m}$ in diameter), intermediate (70-140 $\mu\textrm{m}$ in diameter) and large (180-200 $\mu\textrm{m}$ in diameter), showed a atage-specific variation. Coelomic fluid proteins (CP), ass$\mu\textrm{m}$ed to be vitellogenin, consists of several proteins, which showed quite a different pattern from that of yolk proteins. Incorporation of $^{125}I$-CP into the oocytes of the intermediate size class almost linearly increases with time, showing a contrast to the pattern of the large size class, in which the incorporation is low and approaches a plateau, suggesting the vitellogenin transports by a regulated process only in the intermediate size class. Vitellogenin receptor proteins were identified to be 60 kDa and 68 kDa only in the intermediate size class by a ligand blotting test.

Regulatgion of the Transport of Vitellogenin by Heterotrimeric G-Proteins during Oogenesis of a Polychaete, Pseudopotamilla occelata

이봉경,이양림,Yi, Bong-Kyung,Lee, Yang-Rim The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.1

Coelomoic fluid protein (CP), a vitellogenin contained in the coelomic fluid of polychaetes, is transported by receptor-mediated endocvtosis that is controlled by GTP-binding proteins. Transport of 125l-CP was markedly inhibited by AlF4 and toxins such as cholera toxin and pertussis toxin. These effects appear to be mediated by cAMP, since 125l-CP transport was also greatly inhibited by dibutyryl cAMP. The results strongly suggest that hetero trimeric G-protein is involved in the regulation of 125l=CP transport through the activation of adenylyl cyclase. Immunoblotting tests with antibodies against Gsa and Gia subunits showed a Gsa subunit of 45 kDa in the membrane of oocytes of intermediate and large size classes and a Gia subunit of 41 kDa only in the oocytes of the intermediate size class.

도시영세가구 노인의 건강과 건강행위에 관한 연구

이봉경 ( Lee Bong Gyeong ),김응익 ( Kim Eung Ig ) 서울대학교 인구의학연구소 1996 人口醫學硏究論集 Vol.9 No.1

도시영세가구 노인의 가족관계 만족도에 관한 연구

이봉경 ( Lee Bong Gyeong ),김응익 ( Kim Eung Ig ) 서울대학교 인구의학연구소 1997 人口醫學硏究論集 Vol.10 No.1

컴퓨터사용과 건강에 관한 연구

이봉경 ( Lee Bong Gyeong ) 서울대학교 인구의학연구소 2000 人口醫學硏究論集 Vol.13 No.1

The Cytoskeletal and Chromosomal Constitution of Vitrified Immature Mouse Oocytes

박세필,이봉경,김은영,남화경,이금실,윤산현,정길생,임진호,Park, Se-Pill,Yi, Bong-Kyung,Kim, Eun-Young,Nam, Hwa-Kyung,Lee, Keum-Sil,Yoon, San-Hyun,Chun, Kil-Saeng,Lim, Jin-Ho The Korean Society for Reproductive Medicine 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.3

연구목적: 본 연구는 동해방지제인 EFS40을 이용한 초자화동결이 생쥐 미성숙란의 cytoskeleton과 염색체의 성상에 미치는 영향을 indirect immunocytochemistry방법과 염색체 분석법으로 알아보고자 실시하였다. 연구재료 및 방법: 본 실험은 생쥐 미성숙란을 EFS40 (40% ethylene glycol, 18% ficou과 0.5 M sucrose가 들어있는 M2배양액)으로 초자화 동결하여 융해한 후 16시간동안 체외 성숙을 유도하여, 제 1극체가 나타난 성숙된 난자를 기준으로 동해제노출군 또는 대조군과 비교 조사하였다. 결과: 초자화동결된 미성숙란의 응해 후 생존율과 체외성숙율은 90.3%과 64.7%로써, 동해제노출군 (86.7%, 69.2%)과 대조군 (100%, 58.3%)에 유사하였다. 초자화동결이 미성숙란의 microtubule과 microfilament에 미치는 영향을 조사하였던 바, 동결군의 microtubule과 micro-filament의 정상적인 형성율 (93.9%, 100.0%)은 동해 제노출군 (94.4%, 100.0%)과 대조군 (100.0%, 100.0%)의 성적과 유사하게 나타났다. 또한, 초자화동결군에서 정상적인 염색체수를 가진 난자의 비율도 65.8%로써, 대조군(79.6%)과 노출군 (69.0%)의 결과와 유의한 차이가 없었다. 결론: 생쥐 미성숙란을 EFS40에 노출하고 동결하는 것이 미성숙란의 cytoskeleton과 염색체성상에 영향을 미치지 않으며, 본 연구에서 사용된 EFS40을 이용한 초자화동결법은 생쥐 미성숙란 동결에 적합하다는 것을 알 수 있었다. This study was to confirm whether the vitrification method using EFS40 freezing solution has detrimental effect on the cytoskeleton and chromosome constitution of the immature mouse oocytes by indirect immunocytochemistry and chromosome analysis. Immature mouse oocytes were vitrified using EFS40 (40% EG, 18% ficoll, 0.5 M sucrose diluted in M2 medium), thawed and then survived oocytes were in vitro matured for 16 hr. When the microtubule morphology and micro filament distribution in vitrified-thawed immature mouse oocytes were examined, normal percentage of two cytoskeleton in vitrified group (93.9 and 100.0%) was not significantly different from that in control (100.0 and 100.0%) and exposed group (94.4 and 100.0%). The rate of oocytes containing a normal chromosome number in vitrified group was 65.8%, this result was not significantly different from that in control (79.6%) and exposed group (69.0%). These results indicated that exposure to cryoprotectant or freezing has not effect on the alteration of cytoskeleton morphology and the chromosome constitution of mouse oocytes and that our vitrification methods using EFS40 freezing solution was suitable for the cryopreservation of immature mouse oocytes.

60세 이상 노인의 손상 및 중독 사망 양상에 관한 연구

박상화,이봉경,박용억 한국보건교육건강증진학회 1997 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-

60세 이상 노인의 손상 및 중독 사망양상에 관한 연구

박상화,이봉경,박용억 대한보건협회 1997 대한보건연구 Vol.23 No.2

본 연구는 통계청 1995년 사망원인 통계년보를 기초자료로 사망외인 사인구조를 분석하였다. 사망의 외인에 의한 100,000명당 연령별 사망률은 연령이 증가함에 따라 사망율도 상승하는 것으로 나타났고 특히 60세 이상부터 증가속도가 높았다. 전체 손상 및 중독에 의한 사망자중 60세 이상 사망자의 점유율에서 추락에 의한 사망의 점유율이 가장 높았다. 60세 이상 총 외인 사망자중 운수사고에 의한 사망점유율이 남녀 공히 가장 높았다. 성별 외인사에 의한 사망위험도(교차비: odds ratio)는 여성을 기준군으로 했을 경우 60-69세군의 교차비는2.736(p<0.001), 70-79군이 1.969,80세 이상군이 2.001(p<0.001)로 모든 연령층에서 남자노인의 사망위험도가 높았다. 피살을 제외한 모든 외인사에서 남자노인의 사망위험도가 높았다. 세부분류 항목중에서 추락에 의한 사망위험도가 60-69세군이 4.115(95% CI: 3.179-5.327, p<0.001)로 가장 높았으며 연령이 증가함에 따라 사망위험도는 감소하였다. 70-79세군에서는 연기, 불 및 화염에 의한 노출에 의한 사망위험도가 2.357, 80세 이상군에서는 자살이 3.187로 나타났다. 손상 및 중독의 사망률은 다른 연령보다 노인연령에서 사망수준이 높음을 볼 수 있었는데, 노인인구 비율의 증가는 손상 및 중독에 의한 사망자의 절대수가 증가할 것으로 예견된다. 각종 사고와 관련한 사망 및 상병은 만성퇴행성 질환과 달리 사전 예방은 사고 발생율 저하에 상당한 영향을 주기 때문에 노인 인구를 위한 다양한 예방 프로그램의 개발이 시급하다고 사료된다. This study was based on data published in 1996 from the 1995 annual report of cause of death statistics of National Statistical Office. Injury is the third leading cause of death in Korea. Death rate of injury increased with age from 66.1 per 100,000 persons among aged 30-34 to 132.5 per 100,000 in aged 60-65. In the percent of injury cases over 60 years of age among total injury death cases, falls was the first leading cause of death(25.9 percent in male and 60.3 percent in female). In the proportion of injury death cases among aged 60 years and over, transport accident was the leading cause of injury(50.5 percent in male and 51.75 percent in female). The second and third cause of injury were falls and self-harm in female, and self-harm and falls in male respectively. The risk of death caused by injury was higher in male than in female among all cause of injury with the exception of assaults. The risk of death from falls was highest in the aged 60-69 years(Odds ratio(OR): 4.115,95% confidence interval(CI: 3.179-5.327, p<0.001), the risk for exposure to smoke, fire and flames in 70-79 years old(OR: 2.357, CI: 1.507-3.687, p<0.01), and the risk for self-harm in older than 80 years (OR: 3.187, CI: 2.202-4.612, p<0.001).