P-35 : 과불화화합물의 장기노출에 의한 송사리의 세대간 생물농축성 평가

이두희 ( Doo Hee Lee ),이재우 ( Jae Woo Lee ),김지은 ( Ji Eun Kim ),엄익춘 ( Ig Chun Eom ),이재안 ( Jea An Lee ),김필제 ( Phil Je Kim ),최경희 ( Kyeung Hee Choi ),이병천 ( Byoung Cheun Lee ) 한국물환경학회(구 한국수질보전학회) 2013 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2013 No.-

3차원형광스펙트럼을 이용한 국내 6개 하수처리장 유입수,방류수의 유기물특성 연구

이두희 ( Doo Hee Lee ),이병천 ( Byoung Chun Lee ),엄익춘 ( Ig Chun Eom ),이재안 ( Jea An Lee ),김필제 ( Phil Je Kim ),윤준헌 ( Jun Heon Yoon ) 한국물환경학회(구 한국수질보전학회) 2011 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2011 No.-

에스트로겐과 다이옥신 수용체 효모를 이용한 내분비계장애 영향 평가

이병천 ( Byoung Cheun Lee ),김수진 ( Sue Jin Kim ),윤준헌 ( Jun Heon Yoon ),김은주 ( Eun Ju Kim ),( Duong NC ),엄익춘 ( Ig Chun Eom ),( Shiraishi F. ),최경희 ( Kyung Hee Choi ) 한국환경분석학회 2010 환경분석과 독성보건 Vol.13 No.2

Estrogenic and dioxin activities in river water and effluents of sewage treatment plants were evaluated by different in vitro assays. The expression of β-galatosidase in yeast cells (Yeast two-hybrid assay) was used as an indicator of pollutants effects. Estrogenic activities were estimated using medaka estrogen receptor (mER) and human estrogen receptor (hER) yeast while the dioxin activities were measured by Aryl hydrocarbon receptor (AhR) yeast. Five samples were collected including two river water (RW) samples and three effluents (MW) samples. Target chemicals were extracted using a series of solvents which are hexane/dichloromethane, acetone/dichloromethane and methanol. Standard dose-response curves were established with 3 estrogenic chemicals (estradiol, nonylphenol and bisphenol A) and 3 dioxin-like chemicals (naphthoflavone, comestrol and equol). The results showed that both estrogen receptors (i.e., mER and hER) were suitable to be applied into the assessment of micropollutants` estrogenicity, in which, the sensitivity of mER to estrogenic activity was higher than that of hER. AhR also showed a suitable sensitivity to target dioxins. Estrogenic activity of MW-1 was highest among the samples in both mER and hER yeast assays. The estrogenic activities of two other effluents samples, however, were lower than that of MW-1 and other RW samples. This indicates that river water may receive estrogenic-polluted water other than the effluents of STPs. Similarly, dioxin activities in RW samples were higher than that of effluents. It is suggested that the untreated agriculture wastewater could be the reason. This study contributes to the establishment and application of risk assessment of micropollutant in the environment.

체외배양 소 난관상피세포의 정자에 대한 결합 및 활력유지능

노상호,이병천,황우석,Roh, Sang-ho,Lee, Byoung-chun,Hwang, Woo-suk 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

The aim of these experiments was to investigate the effects of bovine oviduct epithelial cells (OEC) derived from different segments to bind sperm binding and maintain their motility in vitro. In experiment 1, the number of sperm attached to OEC derived from isthmus or ampulla, the motility of unattached sperm during co-culture and fertilizing ability were assessed. In experiment 2, heparin treated sperm (hsp) or no treated sperm (nsp) were used to evaluate OEC binding ability of capacitated sperm. In experiment 1, regardless of their origin, approximately 65% of the sperm were attached to OEC within 2h. From 6h of co-culture, the numbers of unattached sperm on ampullary OEC were significantly higher than those on isthmic OEC (p<0.005). From 12h of co-culture, the motility of unattached sperm on isthmic OEC were significantly higher than those on ampullary OEC(p<0.05). The cleavage rate of oocytes inseminated on OEC derived from isthmic segment was also significantly higher than those from ampullary segment (p<0.01). In experiment 2, the numbers of unattached hsp on OEC were significantly higher than those of controls (p<0.01), between 2-24h examination. From 12h of co-culture, the motility of unattached nsp were significantly greater than those of hsp (p<0.01). These results show that bovine OEC derived from the isthmus play more important role(s) for sperm binding, maintaining motility and fertilization in vitro than those from the ampulla, and heparin induced capacitation may change sperm binding ability on OEC in vitro.

Bioaccumulation and histological responses of the common carp, Cyprinus carpio exposed to zinc oxide nanoparticles

Jae-woo Lee(이재우),Jisung Ryu(류지성),Ji-eun Kim(김지은),Yu-jin Shin(신유진),Kyung-tae Kim(김경태),Byoung-cheun Lee(이병천),Kyunghwa Park(박경화),Ig-chun Eom(엄익춘),Pilje Kim(김필제),Kyunghee Choi(최경희) 환경독성보건학회 2014 한국독성학회 심포지움 및 학술발표회 Vol.2014 No.11

흐름 장-흐름 분획기를 이용한 나노물질의 분리

김성희(Sung Hee Kim),이우춘(Woo Chun Lee),김순오(Soon Oh Kim),나소영(So Young Na),김현아(Hyun A Kim),이병태(Byung Tae Lee),이병천(Byoung Cheun Lee),엄익춘(Ig Chun Eom) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.11

최근 들어 나노기술의 발전에 따라 다양한 산업 및 상업분야에서 나노물질의 사용이 급증하고 있다. 이러한 나노물질이 환경에 유출되어 사람의 건강 및 생태계에 악영향을 줄 수 있다는 문제가 지속적으로 제기되어 왔다. 이에 따라 다양한 환경매질 내에서 나노물질의 거동특성 및 생독성 등에 대한 평가들이 요구되어진다. 이러한 평가들이 진행되기 위해서 필연적으로 다양한 매체 내에서 존재하는 나노물질을 효과적으로 분리하고 이들의 특성을 정량화하는 기술들이 수반되어야 한다. 본 논문에서는 이러한 나노물질의 분리기술들 중 시료의 교란이 비교적 적고 전처리과정이 단순한 장-흐름 분획기술을 이용한나노물질 분리에 대한 국내외 선행연구들을 살펴보았다. 특히 가장 많이 활용되어온 흐름 장-흐름 분획기를 중심으로 기본원리를 살펴보고 분리대상인 나노물질의 종류에 따라서 지금까지 수행되어온 연구들을 분류하고 분석해 봄으로써 이 분야의 연구자들에게 실제적인 정보를 제공하고자 한다. 장-흐름 분획기를 이용하여 다양한 환경매질로부터 나노물질을 효과적으로 분리하기 위해서 분리대상인 나노물질의 종류와 특성을 고려한 전처리, 적절한 멤브레인과 운반용액의 선택, 흐름조건의 최적화 등이 중요한 것으로 조사되었다. 뿐만 아니라 분리 후 나노물질의 특성을 정량화하기 위해서 다양한 검출기 및 분석기와의 연계가 필수적으로 보인다. 하지만 아직까지 일부 환경매질에만 국한되어 연구가 진행되었으며, 또한 분리대상인 나노물질의 종류도 극히 제한적인 것으로 조사되었다. 특히 이러한 나노물질에 대한 분리 및 측정에 대한 국내 연구는 매우 부족한 실정이며 나노물질 사용량이 급증하고 있는 현실을 고려하면 이 분야에 대한 연구가 절실히 요구된다. Recently, the consumption of nanomaterials has been significantly increased in both industrial and commercial sectors, as a result of steady advancement in the nano-technologies. This ubiquitous use of nanomaterials has brought up the concern that their exposure to environments may cause detrimental effects on human health as well as natural ecosystems, and it is required to characterize their behavior in various environmental media and to evaluate their ecotoxicity. For the sake of accomplishing those assessments, the development of methods to effectively separate them from diverse media and to quantify their properties should be requisitely accompanied. Among a number of separation techniques developed so far, this study focuses on Field-Flow Fractionation (FFF) because of its strengths, such as relatively less disturbance of samples and simple pretreatment, and we review overseas and domestic literatures on the separation of nanomaterials using the FFF technique. In particular, researches with Flow Field-Flow Fractionation (FlFFF) are highlighted due to its most frequent application among FFF techniques. The basic principle of the FlFFF is briefly introduced and the studies conducted so far are classified and scrutinized based on the sort of target nanomaterials for the purpose of furnishing practical data and information for the researchers struggling in this field. The literature review suggests that the operational conditions, such as pretreatment, selection of membrane and carrier solution, and rate (velocity) of each flow, should be optimized in order to effectively separate them from various matrices using the FFF technique. Moreover, it seems to be a prerequisite to couple or hyphenate with several detectors and analyzers for quantification of their properties after their separation using the FFF. However, its application has been restricted regarding the types of target nanomaterials and environmental media. Furthermore, domestic literature data on both separation and characterization of nanomaterials are extremely limited. Taking into account the overwhelmingly increasing consumption of nanomaterials, the efforts for the area seem to be greatly urgent.

Microsatellite 대립유전자 분석을 통한 개에서의 친자감별

채영진,김동근,김하나,이문한,황우석,이병천,윤화영,이항,Chae, Young-jin,Kim, Dong-keon,Kim, Hana,Lee, Moon-han,Hwang, Woo-suk,Lee, Byoung-chun,Youn, Hwa-young,Lee, Hang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1

Microsatellite allele analysis has been used for individual identification and paternity test. In the present study, the biological father of three puppies was determined by using microsatellite allele amplification analysis. The mother bitch of the litter was a Poongsan dog. The three stud dogs that could have inseminated the bitch, by being in the same residence, were a white Poosan dog, a mixed breed, and a white Jindo dog. DNA was obtained from all the relevant dogs by buccal swabbing. Four loci of tetranucleotide repeat microsatellite were PCR-amplified, and analyzed by polyacrylamide gel electrophoresis and silver staining. The results of genotyping unambigously assigned the Poongsan dog as the biological father. There was no evidence of superfecundation. Therefore, the present study demonstrated the usefulness of microsatellite allele analysis as a simple, efficient method of paternity test in dogs.

실시간 모드의 유도결합플라즈마 질량분석기술을 이용한 금속나노입자의 분석

김현아 ( Hyun A Kim ),나소영 ( So Young Na ),이병태 ( Byung Tae Lee ),김경웅 ( Kyoung Woong Kim ),이우춘 ( Woo Chun Lee ),김순오 ( Soon Oh Kim ),이병천 ( Byoung Chun Lee ) 한국환경분석학회 2013 환경분석과 독성보건 Vol.16 No.4

나노입자의 환경 유출에 따른 인체 및 생태계에 미치는 독성 문제가 제기됨에 따라 나노입자의 환경 안정성에 대한 우려가 증대되고 있다. 다양한 나노 연구분야중에서도 환경 시료 내 나노입자의 검출 및 분석에 대한 연구가 최우선과제로 인식되고 있다. 유도결합플라즈마 질량분석기(ICP-MS)는 무기물질에 대한 정확하고 정밀한 분석기술로서, 환경 시료 내에 존재하는 금속나노입자의 검출을 위한 유망한 분석기술이다. 이에, 본 연구에서는 실시간 모드의 유도결합플라즈마 질량분석기술(RTSP-ICP-MS)의 원리 및 분석절차를 소개하였으며, 구연산(citrate)으로 코팅된 은나노입자를 대상으로 분석기술의 적용 가능성을 평가하였다. RTSP-ICP-MS 분석 결과, 60 nm와 100 nm 은나노입자의 입자수 기준(number-weighted) 평균 크기는 53.0±7.4 nm와 103.6±13.8 nm였다. RTSP-ICP-MS 분석결과를 검증하기 위해 전자투과현미경(TEM) 분석을 실시한 결과 입자의 평균크기는 각각 60.1±3.7 nm와 96.8±9.5 nm였고, 동적산란기(DLS) 분석을 통한 z-average 값은 59.1±0.2 nm와 95.3±0.8 nm로 나타났다. 다양한 기술을 적용한 은나노입자의 크기분석 결과를 통해 RTSP-ICP-MS 기술을 이용한 금속나노입자의 분석가능성을 검증하였다. 향후, 다양한 환경 매질(담수, 폐수, 슬러지, 퇴적물 및 토양 등)내에 존재하는 금속나노입자의 검출 및 분석을 위한 향후 연구가 필요하다. Significant concern has been laid on the environmental safety of nanoparticles due to their exposure and toxicity to human and ecosystems. Among the research topics, detection and analysis of nanoparticles in the environmental matrix is regarded as the most important and pressing work. Owing to its precise and accurate analytical power to inorganics, inductively coupled plasma mass spectrometry (ICP-MS) is a very promising tool to detect metal nanoparticles in the environmental samples. Here, we introduced the principles and procedures of on Realtime Single Particle mode ICP-MS (RTSP-ICP-MS) technique and evaluated its applicability to citrate-coated silver nanoparticles (AgNPs). RTSP-ICP-MS determined AgNPs of 60 nm and 100 nm to 53.0 ±7.4 nm and 103.6±13.8 nm, respectively, which were the number-weighted average sizes. Comparative measurements suggested that sizes by Transmission Electron Microscopy (TEM) were 60.1±3.7 nm and 96.8±9.5 nm and z-averages by Dynamic Light Scattering (DLS) were 59.1±0.2 nm and 95.3±0.8 nm, respectively. The results prove the feasibility of RTSP-ICP-MS for metal-nanoparticles. We have many challenges to be overcome for detection and measurement of metal nanoparticles in environmental samples such as fresh water, wastewater, sludges, sediments and soils.

굴포천에 서식하는 어류체내 과불화화합물 농축특성

조천래(Chonrae Cho),조재구(Jae-Gu Cho),엄익춘(Ig-chun Eom),이병천(Byoung-cheun Lee),김수진(Sue-jin Kim),최경희(Kyunghee Choi),윤준헌(Junheon Yoon) 환경독성보건학회 2010 환경독성보건학회지 Vol.25 No.3

During the last decade, perfluorinated compounds (PFCs) have gained more attention due to their toxicity and global distribution. The aim of this study was to examine the distribution and bioaccumulation of perfluorinated compounds (PFCs) in aquatic wildlife effected from a sewage treatment plant. The concentrations of 12 PFCs were determined in water, sediment and fish samples. PFOS were predominantly detected in both ambient environment and fish. In fish, the concentration of PFCs in blood was the highest (i.e., 112.47 ng/㎖ wet-wt. PFOS) in comparison to other tissues. However, PFOA and PFHpS were highly detected in gonad as 3.87 and 4.58 ng/g wet-wt., respectively. The bioconcentration factor (BCF) of PFCs was greatest in the blood> liver?gonad > kidney> gill, and lowest in the muscle tissue. The BCFs of PFUnDA (39,000), PFDA(2.700) and PFOS (1,100) were rated as high values based on wet weight concentration. BCFs increased with increasing the length of the perfluoralkyl chain.

한 農村地域의 寄生蟲感染에 對한 疫學的 調査

李炳千,盧忍圭 서울大學校 保建大學院 1974 公衆保健雜誌 Vol.11 No.1

A survey on helminthic infections in a rural area of Korea, Dongnae Area, Shindong Myon, Chunseong Gun, Kangwon-do (Province) was undertaken to evaluate the status of infection, providing the basic data for control of parasitic infection. The survey has been conducted from March to September, 1973 on the 3,454 inhabitants (47.2%) of 7,314 of the area. Cellophane thick smear technique was appled for stool examination. The data were analysed and showed the following results: 1) The overall prevalence rate of helminthic infections by stool examination was 75.7% (male 73.7%, female 77.6%). The rate of each helminth was as follows: Ascaris lumbricoides 61.9%, Trichuris trichiura 41.9%, hookworm 1.0%, Trichostrongylus orientalis 0.5%, Enterobius vermicularis 0.3%, Clonorchis sinensis 0.2%, and Taenia species 0.8%. 2) The infection status of Ascaris lumbricoides by occupation of surveyed population revealed significant differences: professional occupation group 11.1%, workers in transport and communication occupation 33.3%, and farmers 50.2%. 3) The infection rate of Ascaris lumbricoides revealed significant difference by the educational background of over 25 years of age group: lower rate in the higher educational level. 4) The overall helminth prevalence rate was not significantly different between the group of the high and low socioeconomic classes. 5) The prevalence rate of Ascaris lumbricoides was significantly higher in agricultural households (65.2%) than in non-agricultural households (50.4%). 6) The familiar aggregation of Ascaris lumbricoides infection among 950 (199 households) population was analysed and revealed highly significant aggregation pattern. And this aggregation was more marked in agricultural households than in non-agricultural households.