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Phytophthora species 의 분자유전학적 분류 및 RAPD fingerprinting 을 이용한 P . infestans - specific 분자마커의 선발
이민웅,김경수,신환성,김희종,우수진,함영일,신관용,이정운,김병섭,심재욱,이윤수 한국균학회 1999 韓國菌學會誌 Vol.27 No.6
Taxonomic and genetic analysis of Phytophthora species belonging to six different morphological groups (GI, GII, GIII, GIV, GV, GVI) was conducted using RAPD method. Amplified fragments ranged 0.3∼3.2 kb in their molecular weights. Among total of 145 bands, there were 109 polymorphic bands Seven isolates of P. infestans showed high similarities of 0.92∼0.99, and P. infestans isolate 3 from potato showed similarities of 0.93∼0.95 compared with other P. infestans. Among isolates of P. capsici, similarities of 0.77∼0.86 were observed and they were grouped in 80% level. P. cinnamomi and P. cryptogea isolates which belonging to group GVI showed very similar RAPD fingerprinting pattern. Primers OPA-04, OPA-17, OPA-18, OPA-19, and OPB-12 showed high level of differences among the tested isolates in major bands and molecular weights. The similarity between the isolates was 0.67. P. megasperma and P. sojae in group GV showed similarity of 0.65. These two isolates showed big differences in single major band in reactions with primers OPA-08, OPA-17, and OPA-19. Phytophthora-specific and P. infestans-specific molecular markers were also selected with one of the random primers tested. In reaction with primer OPA-20, all the genus Phytophthora showed common band at 600 bp, and all the P. infestans isolates showed specific band at 680 bp. These markers can be useful far identification of Phytophthora speices or P. infestans. As a result, P. infestans isolated from tomato and/or potato can easily be differentiated from other Phytophthora species with this primer.
이태수,이상선,박동규,가강현,이민웅,이윤수,심재욱,손서규,반기원,김광포 한국균학회 1998 韓國菌學會誌 Vol.26 No.3
This study was carried out to obtain the basic data for artificial production of Polyporus umbellatus. Among logs consisted of 16 tree species, the log of Stylax japonica inoculated with Polyporus umbellatus not only produced compact sclerotia of 3∼5 ㎜ in their diameters but resulted in the good mycelial density. Also, the sclerotia were observed on 6 different kinds of logs inoculated with Polyporus umbellatus.