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홍재식,이종배,고무석,김정숙,이극로,정기태,Hong, Jai-Sik,Lee, Jong-Bae,Koh, Moo-Seok,Kim, Jeong-Sook,Lee, Keug-Ro,Jung, Gi-Tae The Korean Society of Mycology 1986 韓國菌學會誌 Vol.14 No.1
Pleurotus spp.중 섬유소(纖維素) 분해효소(分解酵素) 생산력(生産力)이 가장 강한 Pleurotus sajor-caju JAFM 1017을 합성배지(合成培地) 상에서 vitamin류(類), 무기염류(無機鹽類)와 배양조건(培養條件)의 영향을 검토(檢討)한 결과(結果)는 다음과 같다. 섬유소(纖維素) 분해효소(分解酵素) 생산(生産)은 folic acid와 thiamine-HCI에 의해 촉진되었고 $KH_2PO_4$와 $MgSO_4$의 최적농도(最適濃度)는 각각 0.2%, 0.04%(w/v)이었으며 그 밖의 무기염류(無機鹽類)는 효과(效果)가 없었다. 효소생산(酵素生産)에 최적(最適)인 배양온도(培養溫度)와 배지(培地) pH는 avicelase가 $25^{\circ}C$, 5.5이었고, CMCase는 $30^{\circ}C$, 5.0이 였으며, ${\beta}-glucosidase$는 $30^{\circ}C$, 6.5이었다. The production of cellulolytic enzymes by Pleurotus sajor-caju JAFM 1017 was stimulated by folic acid and thiamine-HCl. Among the inorganic salts, optimum concentrations of $KH_2PO_4$ and $MgSO_4{\cdot}7H_2O$ were 0.2% (w/v) and 0.04% (w/v), respectively, but other inorganic salts were not effective for the production of the enzymes. The optimum culture temperature and pH for the production were $25^{\circ}C$ and 5.5 for avicelase, and $30^{\circ}C$ and 5.0 for CMCase, and $30^{\circ}C$ and 6.5 for ${\beta}-glucosidase$, respectively.
홍재식,김명곤,김영회,이극로,이지열,정기태 한국균학회 1985 韓國菌學會誌 Vol.14 No.1
The volatile aroma concentrates were isolated from Pleurotus ostreatus by simultaneous distillation-extraction and analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The aroma concentrates obtained had a typical raw or slightly cooked mushroom-like odor, and the yield was 123 ppm. Of 27 components identified, the major components were 3-octanone, 3-octanol, 2-octenal, n-octanol and 2, 4-decadienal, including 1-octen-3-ol contributing 67.06% of the total volatiles, and the C_8 compounds comprised about 80% of the total volatiles. On the other hand, the C_6 and C_8 alcohols in mushroom increased gradually with increase of standing time after homogenation, while aldehydes, ketones, and linoleic acid decreased, and also C_6 and C_8 compounds were significantly increased when linoleic and linolenic acid were added in homogenates. It appears that most of volatile aroma components in Pleurotus ostreatus were probably produced enzymatically from unsaturated fatty acids containing cis, cis-1, 4-pentadiene moiety.
합성 배지에서 느타리속이 생산하는 섬유소 분해효소에 관한 연구 (제2보)
김정숙,이종배,홍재식,고무석,이극로,정기태 한국균학회 1985 韓國菌學會誌 Vol.14 No.1
The production of cellulolytic enzymes by Pleurotus sajor-caju JAFM 1017 was stimulated by folic acid and thiamine-HCI. Among the inorganic salts, optimum concentrations of KH₂PO₄ and MgSO₄·7H₂O were 0.2% (w/v) and 0.04 (w/v), respectively, but other inorganic salts were not effective for the production of the enzymes. The optimum culture temperature and pH for the production were 25℃ and 5.5 for avicelase, and 30℃ and 5.0 for CMCase, and 30℃ and 6.5 for β-glucosidase, respectively.
김동한,이태규,김명곤,홍재식,이극로,김중만,정진철 한국균학회 1985 韓國菌學會誌 Vol.13 No.3
To investigate nutritive values of a feed fermented with basidiomycetes, among the isolated strains, Lyophyllum decastes (Fr.) Sing. was found with the greatest enzyme productivity and rapid mycelial growth in rice straw medium. Optimum temperature, pH and moisture content for mycelial growth arid enzyme production of the strain were 25∼30℃, pH 4.0∼7.0 and 70∼75%, respectively. Fifteen days of culture were required for the highest enzyme productivity. Among the sub-materials added, 30∼40% of rice bran and 10∼20% of defatted perilla seeds were effective for the enzyme production, but caused a reduced mycelial growth. The greatest effect of an addition of inorganic salts was obtained with 0.36∼0.72 % of (NH₄)₂HPO₄. When 40 mesh or smaller rice straw and steam treatment at 0.5 ㎏/㎠ were used, the mycelial growth decreased, whereas the enzyme production increased. The mycelial growth and enzyme production increased when Ca(OH)₂ was used as the alkali treatment, but decreased with increasing concentration of NaOH. As the fermentation proceeded, the amounts of ash, reducing sugar and total nitrogen increased, but cellulose, lignin and pentosan decreased. When the rice straw was treated with alkali, the amounts of ash, total nitrogen and lignin decreased, but reducing sugar and cellylose increased. At higher NaOH concentration, the variation become greater. The in vitro dry matter digestibility of the products increased from 55.03% at the beginning of the fermentation to 62.72% at 45 days after fermentation. The most effective alkali treatment on the digestibility of rice straw was KOH followed by NaOH. However, the digestibility increased with increasing concentration of NaOH. The digestibility of pretreated with alkali increased after fermentation as well.
합성배지에서 Pleurotus 속이 생산하는 섬유소 분해효소에 관한 연구 (제1보) : 탄소원과 질소원의 영향 Effects of Carbon and Nitrogen Sources
김정숙,이종배,홍재식,김명곤,고무석,이극로 한국균학회 1985 韓國菌學會誌 Vol.13 No.4
Among the eight strains, Pleurotus sajor-caju JAFM 1017 was selected as most potent producer of cellulolytic enzymes. The avicelase and CMCase activity reached maximum levels after 10 days, and β-glucosidase activity reached a maximum level after 19 days. Among the various carbon sources, cellulose powder was most effective for the production of avicelase and β-glucosidase, and Na-CMC (sodium carboxymethyl cellulose) was good for the production of CMCase. The optimum concentration of cellulose powder was 1.0% (w/v), and glucose (1.0%) completely depressed the production of enzymes. Nitrates were effective for the production of enzymes, but nitrites did not support growth. The production of cellulolytic enzymes increased as the concentration of urea increased. The appropriate concentration of urea was 0.054% (w/v).