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On-line Screening HPLC-ABTS<sup>+</sup> Assay을 이용한 산청목으로부터 Salidroside의 분리 및 생물활성 분석
이광진,송나영,마진열,Lee, Kwang Jin,Song, Na-Young,Ma, Jin Yeul 한국생물공학회 2014 KSBB Journal Vol.29 No.2
Acer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as on-line screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase fromAcer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as online screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase from 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum. 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum.
이광진,김상혁,권의표,손규송,Lee, Kwang-Jin,Kim, Sang-Hyuk,Kwon, Eui-Pyo,Son, Kyu-Song 대한용접접합학회 2014 대한용접·접합학회지 Vol.32 No.2
Friction stir welding (FSW) was successfully performed about 900MPa grade Twinning Induced Plastisity (TWIP) steel. A PCBN tool with convex-type shoulder was applied. Optimal process conditions were deduced. Microstructure and mechanical properties such as hardness, tensile strength and impact absorbed energy were observed and evaluated, respectively. An optical microscope (OM) and a field emission scanning electron microscope (FE-SEM) was selected for observing the grain structure.
이광진,김형자,Kwang-Jin, Lee,Hyung-Ja, Kim 한국기계가공학회 2004 한국기계가공학회지 Vol.3 No.3
The sub-surface stress field beneath the spur gear's contact surface in lubricated condition has been analysed. The surface pressure was obtained by the elasto-hydrodynamic lubrication analysis using the accurate geometric clearances around the contact region of the teeth. The sub-surface stress field was calculated by using the Love's rectangular patch solution. The analysis results show that the sub-surface stress distribution is quite dependent on the surface pressure distribution. The pattern of sub-surface stress field is similar to that of the external load. The depth where the maximum effective stress occurs is not proportional to the intensity of the external load.
Al-Mg-Si계 알루미늄 합금 판재 마찰교반접합부의 결정 방위 분포에 대한 용접후열처리의 영향
이광진,Lee, Kwang-Jin 대한용접접합학회 2009 대한용접·접합학회지 Vol.27 No.6
Friction stir welding (FSW) was carried out for Al-Mg-Si series aluminum alloys which are being used for automotive body structure. Consequently, Post weld heat treatment (PWHT) was applied to the friction stir welds to evaluate the effect of the paint baking process which is one of the automotive fabrication process on friction stir welded zone (FSWZ) in 443K for 1.2Ks. Grain structure and its crystal orientation distribution was measured about both the as welded specimens and the post weld heat treated specimens. An optical microscope (OM) and an field emission scanning electron microscope (FE-SEM) was used for observing the grain structure and measuring its crystal orientation distribution, respectively. Changes on the grain structure and its crystal orientation distribution were not detected. From the present results, it was confirmed that the paint baking process after FSW do not affect on the grain structure and its crystal orientation distribution of FSWZ. The comprehensive investigations will be performed for various automotive aluminum alloys manufactured by different processes, in the future.
마찰교반용접(FSW) 및 마찰교반처리(FSP)의 최신 연구개발 동향
이광진,Lee, Kwang-Jin 대한용접접합학회 2013 대한용접·접합학회지 Vol.31 No.2
The latest research & development trend on friction stir welding and friction stir processing technologies presented in the international symposium, 'Friction Stir Welding & Processing VII'. Papers and presentations about high temperature materials such as advanced high strength steel, stainless steel and titanum alloy shoot up this year. Papers on modeling of metal flow and control of process parameters also increased. The FSP technologies for manufacturing of carbon materials reinforced metal matrix composites were reported, too.
A6061-T6/AZ31 이종금속 마찰교반접합부의 기계적 특성에 미치는 툴 삽입 위치의 영향
이광진,김상혁,Lee, Kwang-Jin,Kim, Sang-Hyuk 대한용접접합학회 2012 대한용접·접합학회지 Vol.30 No.5
Butt-joints of A6061-T6 and AZ31 plates were successfully manufactured by using a friction stir welding method. Off-set free joints and off-set joints were fabricated to investigate the effect of the tool plunge position on the mechanical properties of the joints. Hardness test, tensile test and charpy impact test was performed to evaluate the mechanical properties of the joints. Off-set condition resulted in increase of mechanical properties of the joints. The variation of the hardness distribution in the SZ was also stabilized in the off-set condition. Tensile strength of the off-set joint was about 85% against to that of the AZ31 base metal. Impact absorption energy by Charpy-Impact-Test of the off-set joint also increased by almost 2 times against to that of the AZ31 base metal.
초음파를 이용한 이대와 왕대 잎으로부터 항산화물질 Homoorientin 의 추출효율 향상
이광진(Kwang Jin Lee),신용국(Yong Kook Shin),김영식(Young Sik Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.2
대나무 (이대와 왕대) 잎으로부터 HPLC on-line ABTS screening기법을 사용하여 flavone C-glycosides의 homoorientin 의 항산화활성을 빠르게 분석하였으며, 이대와 왕대 잎으로부터 homoorientin의 추출을 다양한 초음파 에너지와 시간의 추출방법을 적용하였다. 전 처리한 추출액에 포함된 이대와 왕대 잎으로부터 homoorientin을 분석하고 최적의 추출조건을 실험적으로 모색하였다. 실험결과에 의하면 왕대는 이대 잎보다 homoorientin의 함량이 7배 정도 높았으며, 최적 추출은 35 kHz, 60 min에서 (positive) 피크면적 1574.71, 23.67%와 (negativity) 피크면적 6924.34, 1.23% 로 왕대 잎에서의 추출 효율이 가장 우수 하였다. In this work, antioxidant substance homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves wereextracted using ultrasonic system, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) on-line ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant screening method. Also, the various experimental variables were the frequency and time of ultrasonic system. From the results, homoorientin was high extracted at the experimental condition of low frequency 35 kHz and time 60 min. And the content of homoorientin in Phyllostachys bambusoides was remarkably higher than that in the Pseudosasa japonica extract.