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소나무 자엽을 (子葉) 이용한 원형질체 나출 (裸出) 및 배양
이경준,이재순,윤양,이석구 ( Kyung Joon Lee,Jae Soon Lee,Yang Youn,Suk Koo Lee ) 한국산림과학회 1985 한국산림과학회지 Vol.71 No.1
Protoplasts were isolated from cotyledons of germinating Pinus densiflora S. et Z. seeds. The seeds were germinated for nine days, and excised embryonic cotyledons about 3-4㎜ long were incubated with Cellulase Onozuka R-10 and Macerozyme. After 8 hours of incubation, large number of viable protoplasts were isolated. Isolated protoplasts were cultured in a medium containing basal salts of BS medium, vitamines, amino acids, organic acid, sugars, and growth hormones. The first; evidence of protoplast budding was observed after twelve hours in culture, and it suggested that high potential of the embryonic cotyledons for rapid cell division affected the early budding, rather than effect of culture medium was shown in twelve hours. The three- to four-cell stage was reached after three to four days of culture. Most cell divisions were achieved by additional buddings rather than equal binary cell division. No further cell division was observed beyond the four-cell stage. Protoplasts isolated from fully expanded cotyledons (germinated for 17 to 24 days) seldom initiated or failed to initiate cell division.
이경준,이돈구,이원규,구창덕 ( Kyung Joon Lee,Don Koo Lee,Won Kyu Lee,Chang Duck Koo ) 한국산림과학회 1983 한국산림과학회지 Vol.59 No.1
Recently mycorrhizal research has been one of the most fast-growing research areas in modern plant science and microbiology. The application potential of mycorrhizal techniques to agriculture and forestry is enormous in view o1` the ubiquitous nature of mycorrhizae and known benefits of mycorrhizae to host plants. Unfortunately, very few scientists in Korea are currently involved in mycorrhizal research. When a team of American plant pathologists visited Korea in September 1982 to participate in the Korea-U.S.A. Joint Seminar on Forest Diseases and Insect Pests, they were surprised by the principal author`s statement that there was no single research project on mycorrhizae sponsored by Korean government or any scientific institutions. The author initiated a few years ago a research project on the ecology of tree mycorrhizae with a foreign financial support. Major areas of interest were survey of ectomycorrhizae in relation to soil fertility, taxonomic distribution of mycorrhizae among woody plants, identification of ectomycorrhizal fungi, and growth response of woody plants to artificial inoculation. In spite of the enormous application potential of mycorrhizae to agronomic plants, the subject of mycorrhizae has not been recognized by Korean agronomists, foresters or pathologists. The purpose of this review rather written in Korean is to introduce the techniques of mycorrhizal research to Korean scientists and to urge them to participate in challenging new scientific field which might bring us a remarkable increase in crop productivity and tree growth through manipulation of this unique symbiosis. In this review, following topics were discussed in the same order: introduction; brief history of mycorrhizal research; .morphology and classification of mycorthizae; distribution of mycorrhizae in plant kingdom and in soil profile; physiology of mycorrhizae (functions, mineral nutrition, mycorrhizal formation): interaction of mycorrhizae with soil-born plant pathogens mycorrhizae in nitrogen-fixing plants ; application of mycorrhizal techniques to nursery practices (isolation, culture, inoculation, and response); prospect in the future.
모래밭버섯 균근균의 (菌根菌) 인공접종에 의한 포플러 삽목묘의 (揷木苗) 생장촉진 및 활착률 증진
이경준,구창덕 ( Kyung Joon Lee,Chang Duck Koo ) 한국산림과학회 1985 한국산림과학회지 Vol.70 No.1
Populus alba × P. glandulosa cuttings in nursery bed were inoculated with mycelium of ectomycorrhizal fungus, Pisolithus tinctorius (Pt) to evaluate effectiveness of the fungus in growth stimulation of poplar. Pt was cultured in 11 glass bottles with vermiculite-peatmoss mixture moistened with modified Melin-Norkrans` solution. The nursery bed was arranged for microplots of 1 × 2m in size and fumigated with methyl bromide before inoculation and cutting placement. Fifty cuttings were placed in each microplot and two treatments (fumigation only and fumigation plus Pt inoculation) were replicated three times. At the end of the first growing season, inoculated plants grew 19% faster in height and produced 49% more dry weight (above-ground portion) than uninoculated plants. Survival rate of inoculated cuttings was also improved by 20% over that of uninoculated cuttings. Inoculated cuttings developed abundant fine root system with golden brown zigzag tips. In the middle of September a sporocarp of Pt was produced from an inoculated plot, suggesting successful establishment of mycorrhiza between poplar and Pt fungus.
한국산과 미국산 모래밭버섯 균근균의 (菌根菌) 리기테다 소나무 파종균에 (播種苗) 대한 포자접종효과 비교
이경준,구창덕 ( Kyung Joon Lee,Chang Duck Koo ) 한국산림과학회 1984 한국산림과학회지 Vol.65 No.1
Pinus rigida × P. taeda seedlings in a nursery was inoculated with basidiospores of Pisolithus tinctorius (Pt) either collected from Suweon, Korea or introduced from U.S.A. to compare the effectiveness of the spores from two different origins as mycorrhizal inocula. Nursery beds were fumigated with methyl bromide and lg of spores was used to inoculate 1㎡ of soil surface just before seed sowing. Seedlings inoculated with American Pt (#250 strain from Georgia, U.S.A.) were 15% taller than Korean Pt at the end of the first growing season. The seedlings from fumigation treatment only (no inoculation involved) was slightly taller (statistically unsignificant) than those with Korean Pt, but slightly smaller than those with American Pt. In a subsequent year experiment, the seedlings inoculated with American and Korean Pt after soil fumigation were 66% and 60% taller, respectively, than seedlings infected by natural fungi without soil fumigation, suggesting the dual effects of Pt and fumigation on the seedling growth. Therefore potential of Pt spores for an effective inoculum exists and selection of Pt strains which have adapted to specific local environments is needed to develop better sources of mycorrhizal inocula.