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이형호,박상대,이갑렬,윤정호 한국유전학회 1994 Genes & Genomics Vol.16 No.2
Eukaryotic DNA is known to be synthesized via replication complexes that are fixed to proteinaceous nuclear complex. We investigated whether the binding of parental DNA to the nuclear matrix is affected by ultraviolet (UV) light in mouse LP1-1 cells. The matrix-binding of parental DNA as determined by DNasc-digestion of halo structures, markedly increased to 7-fold of control al 2hr after irradiation (10J/㎡) and then decreased as a time dependent manner. These enhanced binding of parental DNA by UV light may suggest that the newly activated replication origins hind transiently to the nuclear matrix. To examine that these enhanced bindings reflect the activation of new replication origin, we determined the rate of DNA synthesis and the sizes of newly replicated DNA. The weight average molecular weights (Mw) of nascent DNA were still smaller than that of control at 2hr after UV-irradiation, while the rate of DNA synthesis completely recovered during the same time period. These results indicate that the new replication origins were activated by UV-irradiation in order to replicate the long-lived unreplicated rregions (LLUR) produced by UV lesions in LP1-1 cells.
서진,김세년,이갑렬,김소영,박상대,홍승환,Seo, Jin,Kim, Se-Nyun,Lee, Gap-Ryol,Kim, So-Young,Park, Sang-Dal,Hong, Seung-Hwan The Korean Society for Integrative Biology 1997 Korean journal of biological sciences Vol.1 No.2
We examined the effect of modulation of PKA isozymes on the growth of human ovarian cancer cells. Three ovarian cancer cell lines, 2774, SK-OV-3, and OVCAR-3, were examined in this study. The treatment of 5 uM 8-CI-cAMP, which has been known to down-regulate RI (or type 1 PKA) and up-regulate RII (or type II PKA), markedly inhibited the growth of all cell lines (50-80% at day 6). To test whether alteration in PKA regulatory subunits level can change the growth characteristics of ovarian cancer cells, we introduced RIIB- expression construct and Rla antisense-expression construct into 2774 cells. The overexpression of RIIB down-regulated Rla protein, and the antisense-expression of Rla up-regulated RIIB protein, showing that the intracellular levels of RI and RII are reciprocally regulated. In both cases, cell growth was reduced by 30% at day 2. These results indicate that the growth of ovarian cancer cells is controlled by the signals from PKA isozymes, and the modulation of PKA isozymes can be employed for the human ovarian cancer therapy.