광전환 필름 피복에 따른 채소류의 묘소질 및 광합성 특성

윤효인(Hyo In Yoon),강준현(Jun Hyeun Kang),김도연(Doyeon Kim),손정익(Jung Eek Son) (사)한국생물환경조절학회 2021 생물환경조절학회지 Vol.30 No.2

본 연구는 광전환필름에 의해 변형된 태양광 하에서 채소류의 묘소질과 광합성 특성에 미치는 영향을 구명하기위해 수행되었다. 상추와 배추, 오이 종자를 원예용 상토로 충진된 50구 플러그 트레이에 파종하고, 광전환 필름(SCF)과 상업용 폴리에틸렌 필름(대조군) 하에서 재배하였다. 대조군 대비 SCF에 투과된 태양광 스펙트럼은 청-녹색광은 감소하고, 적색광은 증가했으며 R/FR 비와 R/B 비가 증가하여 묘의 광합성과 생육, 형태형성 측면에서 영향을 미치기에 충분한 수준이었다. 광합성속도는 SCF 하에서 자란 배추와 오이 묘에서 유의적으로 증가했으며, 최대 광합성속도는 각각 23%, 19% 높게 나타났다. 상추의 경우, SCF 처리구에서 생육이 유의적으로 감소하였으며 도장하였다. SCF 필름 하에서 자란 배추의 경우, 형태적인 차이는 없었으나 잎의 건물중이 증가하여 묘의 충실도가 유의적으로 증가했고 왜화율은 10%로 나타났다. SCF 처리구의 오이 묘는 생육에 차이는 없었으나 엽병 및 초장이 유의적으로 감소하여 왜화율이 24%로 높게 나타났다. 결론적으로 SCF에 의해 변형된 태양광 스펙트럼은 배추와 오이 묘의 광합성 효율을 증가시키고 묘소질을 향상시켰다고 판단되었다. This study aims to investigate the effect of spectrum conversion film on seedling quality and photosynthetic traits of leaf and fruit vegetables. Lettuce, Chinese cabbage, and cucumber seeds were sown in 50-cell plug trays filled with commercial seedling media and grown under a spectrum conversion film (SCF) and a poly-ethylene film as a control. The SCF decreased blue-green light and increased red light, and thereby increased the R/FR ratio and R/B ratio of solar spectrum, which was sufficient to affect the photosynthesis, growth, and morphogenesis of the seedlings. The photosynthetic rates of Chinese cabbage and cucumber were significantly increased under the SCF, and their maximum photosynthetic rates were 23% and 19%, respectively. However, the growth of lettuce was decreased under the SCF, and the plant height and leaf length became longer, resulting in a decrease in seedling quality. Although the morphology of Chinese cabbage grown under the SCF was not changed compared to the control, the leaf dry weight increased and its dwarf rate was 10%. The growth of cucumber under the SCF was similar to that of the control, but the plant height and petiole were significantly reduced, resulting in a high dwarf rate of 24%. It was concluded that the solar spectrum modified by the SCF increased the photosynthetic efficiency and improved the seedling quality of Chinese cabbage and cucumber.

Epigallocatechin Gallate (EGCG)의 포름알데히드에 의한 폐 대식세포 사멸 차단 효과

오영준(Yeong Joon Oh),최주희(Joo Hee Choi),윤효인(Hyo In Yoon),박수현(Soo-Hyun Park) 한국차학회 2015 한국차학회지 Vol.21 No.2

The consumption of green tea (GT) is linked to a decrease in the onset of diverse diseases in humans. Epigallocatechin-3-gallate (EGCG) is the major catechin of green tea. Formaldehyde (FA) is an important irritant compound in pesticides to induce asthma and allergy in the respiratory system. Alveolar macrophages are also a pivotal cell type in the immune response of the respiratory system. On the other hand, the effects of EGCG on FA-induced cell death has not been elucidated. Therefore, this study examined the preventive effects of EGCG against FA-induced cell death in Raw 264.7 cells, an alveolar macrophage cell line. In the present study, FA induced a decrease in the cell viability and GSH contents, and stimulated lipid peroxide [LPO] formation. A treatment with green tea extracts (GTE) and EGCG (> 1 μM) prevented FA-induced cell death, lipid peroxide formation, and GSH contents in Raw 264.7 cells. The treatment with GTE and EGCG also prevented the FA-induced increase of cleaved caspase-3 and Bax, pro-apoptotic proteins, and a decrease in Bcl-2, an anti-apoptotic protein in Raw 264.7 cells. Hence, it is expected that EGCG from green tea offers protection against FA-induced cell death and its main action is mediated by a decrease in oxidative stress. In conclusion, green tea extracts prevented FA-induced cell death by decreasing the levels of oxidative stress in Raw 264.7 cells.

Epigallocatechin Gallate (EGCG)의 벤젠에 의한 폐 대식세포 사멸 차단 효과

오영준(Yeong Joon Oh),최주희(Joo Hee Choi),윤효인(Hyo In Yoon),박수현(Soo-Hyun Park) 한국차학회 2015 한국차학회지 Vol.21 No.2

Benzene is a volatile environmental pollutant that induces asthma and allergy in the respiratory system. Epigallocatechin-3-gallate (EGCG) is the major catechin of green tea. Alveolar macrophages play a pivotal role in the immune response of the respiratory system. On the other hand, the preventive effects of EGCG against benzene-induced cell death have not been elucidated. Therefore, this study examined the preventive effect of EGCG against benzene-induced cell death in Raw 264.7 cells, an alveolar macrophage cell line. In the present study, benzene induced a decrease in cell viability, glutathione (GSH) content and stimulated lipid peroxide (LPO) formation. The treatment with the green tea extracts and EGCG (> 1 μM) prevented benzene-induced cell death, and reduced the GSH contents and lipid peroxide formation in Raw 264.7 cells. On the other hand, the total ginseng and capsaicin did not block benzene-induced cell viability, glutathione contents (GSH) and LPO formation. The treatment of GTE and EGCG also prevented the FA-induced an increase in cleaved caspase-3 and Bax, which are pro-apoptotic proteins, and a decrease in Bcl-2, an anti-apoptotic protein in Raw 264.7 cells. Therefore, it is expected that EGCG protects against the benzene-induced cell death and its main action is mediated by the decrease in oxidative stress In conclusion, EGCG prevents benzene-induced cell death by the decreasing the levels in oxidative stress in Raw 264.7 cells.

Estimation of Total Phenolic Contents based on UV-B Radiation Interception in 3D Structure of Kale Leaves under Controlled Environment

Hyo In Yoon(윤효인),Jaewoo Kim,Myung-Min Oh,Jung Eek Son 한국원예학회 2022 한국원예학회 학술발표요지 Vol.2022 No.5

광도, 온도, 생육 시기에 따른 식물공장 모듈 재배 로메인 상추의 3 변수 군락 광합성 모델 개발

정대호(Dae Ho Jung),윤효인(Hyo In Yoon),손정익(Jung Eek Son) (사)한국생물환경조절학회 2017 생물환경조절학회지 Vol.26 No.4

광도와 온도 같은 환경 요인에 의해 광합성 속도가 변화하기도 하며, 생육 시기에 따른 광합성 효율의 변화가 수반되기도 한다. 본 연구에서는 흑로메인 상추(Lactuca sativa L., Asia Heuk romaine)를 이용하여 광도와 온도, 생육 시기에 따른 군락 광합성 속도를 표현하는 두 모델을 구축하고 비교하는 것을 목표로 하였다. 군락 광합성은 정식 후 4, 7, 14, 21, 28 일차 상추를 아크릴 챔버(1.0 × 0.8 × 0.5m)에 넣어 측정하였으며, 이 때 챔버내부의 온도는 19℃에서 28℃까지 변화시켰고 광원은 LED를 이용하여 50에서 500μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>까지 변화시키며 실험하였다. 챔버 내부의 초기 이산화탄소 농도는 2,000μmol·mol<SUP>-1</SUP>로 설정하였으며, 시간에 따른 이산화탄소 농도의 변화율을 이용하여 군락 광합성 속도를 계산하였다. 각 환경요인을 표현하는 3개 식을 곱하여 만든 단순곱 모델을 구성하였다. 이와 동시에 온도와 생육 시기에 따라 변화하는 광화학 이용효율과 카르복실화 컨덕턴스, 호흡에 의한 이산화탄소 발생 속도를 포함하는 수정된 직각쌍곡선 모델을 구성하여 단순곱 모델과 비교하였다. 검증 결과 단순곱 모델은 0.849의 R² 값을 나타내었으며, 수정된 직각쌍곡선 모델은 0.861의 R² 값을 나타내었다. 수정된 직각쌍곡선 모델이 단순곱 모델에 비해 환경 요인(광도, 온도), 생육 요인(생육 시기)에 따른 군락 광합성 속도를 표현하는 데 더욱 적합한 모델인 것으로 판단하였다. The photosynthetic rates of crops depend on growth environment factors, such as light intensity and temperature, and their photosynthetic efficiencies vary with growth stage. The objective of this study was to compare two different models expressing canopy photosynthetic rates of romaine lettuce (Lactuca sativa L., cv. Asia Heuk romaine) using three variables of light intensity, temperature, and growth stage. The canopy photosynthetic rates of the plants were measured 4, 7, 14, 21, and 28 days after transplanting at closed acrylic chambers (1.0 × 0.8 × 0.5 m) using light-emitting diodes, in which indoor temperature and light intensity were designed to change from 19 to 28? and 50 to 500 μmol·m<SUP>-2</SUP>·s<SUP>-1</SUP>, respectively. At an initial CO₂ concentration of 2,000 μmol·mol<SUP>-1</SUP>, the canopy photosynthetic rate began to be calculated with CO₂ decrement over time. A simple multiplication model expressed by simply multiplying three single-variable models and a modified rectangular hyperbola model were compared. The modified rectangular hyperbola model additionally included photochemical efficiency, carboxylation conductance, and dark respiration which vary with temperature and growth stage. In validation, R² value was 0.849 in the simple multiplication model, while it increased to 0.861 in the modified rectangular hyperbola model. It was found that the modified rectangular hyperbola model was more suitable than the simple multiplication model in expressing the canopy photosynthetic rates affected by environmental factors (light Intensity and temperature) and growth factor (growth stage) in plant factory modules.

국내 논토양 및 밭토양 중 농약유래 잔류성유기오염물질의 노출량 평가

임성진,오영탁,노진호,김승용,주형곤,이민호,윤효인,최근형,류송희,박병준,Lim, Sung-Jin,Oh, Young-Tak,Ro, Jin-Ho,Kim, Seung-Yong,Joo, Hyeong-Gon,Lee, Min-Ho,Yoon, Hyo-In,Choi, Geun-Hyoung,Ryu, Song-Hee,Park, Byung-Jun 한국환경농학회 2017 한국환경농학회지 Vol.36 No.3

BACKGROUND:This study was conducted to investigate residual organochlorine pesticides (ROCPs) in agricultural soils and crops. Agricultural soil samples and crop samples were collected from 93 cities and counties. METHODS AND RESULTS: Extraction and clean-up for the quantitative analysis of ROCPs were conducted by the modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. Recovery and limit of detection (LOD) of ROCPs in agriculturalsoils and crops were 76.5-103.0 and 75.2-93.2%, 0.01-0.08 and $0.10-0.15{\mu}g/kg$, respectively. Detected ROCPs in agricultural soils were ${\alpha}$-endosulfan, ${\beta}$-endosulfan, and endosulfan sulfate, the residue were 2.0-12.0, 1.2-53.1, and $2.2-329.8{\mu}g/kg$, respectively. But these pesticides in all green perilla leaf and green pepper samples were not detected. CONCLUSION: These results showed that ROCPs residues in agricultural soils were not as high as crop safety threatening.

포도 및 복숭아 과수원 토양 및 과실 중 잔류성유기염소계 농약류 잔류량 조사

임성진(Sung-Jin Lim),박정훤(Jeong-Hwon Park),노진호(Jin-Ho Ro),오영탁(Young-Tak Oh),주형곤(Hyeong-Gon Joo),이민호(Min-Ho Lee),윤효인(Hyo-In Yoon),최근형(Geun-Hyoung Choi),류송희(Song-Hee Ryu),박병준(Byung-Jun Park) 한국농약과학회 2018 농약과학회지 Vol.22 No.4

LC-QTOF-MS를 활용한 현미 중 동물용의약품의 정량 분석

임성진(Sung-Jin Lim),오영탁(Young-Tak Oh),노진호(Jin-Ho Ro),주형곤(Hyeong-Gon Joo),이민호(Min-Ho Lee),윤효인(Hyo-In Yoon),최근형(Geun-Hyoung Choi),류송희(Song-Hee Ryu),김단비(Dan-Bi Kim),박병준(Byung-Jun Park) 한국농약과학회 2017 농약과학회지 Vol.21 No.3

비글개에서 l-muscone의 급성독성 및 아급성독성시험 연구

유아선,권오경,성하정,곽형일,방명주,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of l-muscone, a major active ingredient of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dosage of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of l-muscone, 0.2㎎/㎏/day(low dosage group), 2 ㎎/㎏/day(middle dosage group), or 20 ㎎/㎏day(high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korean Food and Drug Administration(1996.4.16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ on oral route for both male and females. In animals administered with l-muscone, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, eye examination, hematology, serum chemistry, organ weight and other findings. No histolopathological lesions were observed in both control and treatment groups. Above data strongly suggest that l-muscone in beagle dogs is considered to be safe.

비글개에서 신우황청심원의 급성 및 아급성독성시험

권오경,성하정,곽형일,방명주,신대희,이진영,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New Woohwangchungsimwon (NWCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NWCH, 160 ㎎/㎏/day(low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NWCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NWCH in beagle dogs is considered to be safe.