고지방 식이 유도 고지혈증에 대한 ChondroT의 혈액 내 지질대사에 미치는 영향

윤찬석 ( Chan Suk Yoon ),김도형 ( Do Hyeong Kim ),나창수 ( Chang Su Na ),정지원 ( Ji Won Jeong ),김지훈 ( Ji Hoon Kim ),김선길 ( Sun Gil Kim ),최지민 ( Ji Min Choi ),김선종 ( Seon Jong Kim ) 한방재활의학과학회 2021 한방재활의학과학회지 Vol.31 No.1

Objectives The objective of the study was to investigate effects of ChondroT by improvement of blood metabolites in high-fat diet (HFD)-induced hyperlipidemia rat model. Methods Sprague-Dawley rats were randomly assigned to intact, control, simvastatin, and CT100, CT200 and CT400 (each n=6). For observing cholesterol change, animals were first fed high fat diet for 5 weeks and then high fat diet and drugs for 3 weeks. At the end of the experiment, total cholesterol, triglyceride, high density lipoprotein- cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were analyzed by obtained blood collection. Further, amplified leptin, peroxisome proliferator activated receptor (PPAR) and adiponectin DNA were observed by reverse transcription polymerase chain reaction analysis. Results Observing the effect of ChondroT on the change of lipid metabolism in hyperlipidemia- induced rats, triglyceride and total cholesterol were significantly decreased in SV100 group, HDL-C was significantly increased in SV100, CT100 and CT200 groups, and LDL-C was significantly decreased in SV100, CT100, CT200 and CT400 groups, compared to the control group. Leptin level in hyperlipidemia-induced rats was significantly decreased in CT100 and CT200 groups, compared to the control group. The effect of ChondroT on adiponectin level in hyperlipidemia-induced rats was significantly increased in SV100, CT100 and CT200 groups. PPAR level in hyperlipidemia- induced rats was significantly decreased in SV100, CT200 and CT400 groups. Platelete activating factor level in hyperlipidemia-induced rats was significantly decreased in CT100 and CT200 groups. Conclusions Based on these results, it could be suggested that ChondroT has certain effects of improving blood metabolites in HFD-induced hyperlipidemia. (J Korean Med Rehabil 2021;31(1):81-93)

즉석편의 식품용 포장재의 전자레인지 가열에 의한 변형 원인 분석

윤찬석(Chan Suk Yoon),홍승인(Seung In Hong),조아름(Ah Reum Cho),이화신(Hwa Shin Lee),박현우(Hyun Woo Park),이근택(Keun Taik Lee) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.1

전자레인지 가열조리용 편의식품류는 레토르트 처리되어 장기간 저장 안정성이 부여되는 장점이 있는 반면, 높은 가열 조건으로 인하여 식품의 품질 저하뿐 아니라 내용물의 흡착에 의한 포장재의 착색이나 오렌지필과 같은 물리적 변화가 발생되기도 한다. 일부 매운 소스 제품들의 전자레인지 가열 조리 시 특정 부위에서 심한 포장재의 열 변형 현상이 확인되었다. 열화상카메라와 광섬유온도계를 이용하여 전자레인지 가열 중 포장재 내부의 온도 분포 및 변화 패턴을 관찰한 결과 내용물의 충진 경계인 액위선과 양 모서리 sealing 부위의 온도가 가장 높이 상승하여 hot spot이 형성되는 것을 확인하였고, 이때 온도는 순간적으로 최대 190℃ 까지 상승하였다. 반면 내용물의 중심부에서는 온도 상승이 매우 느리게 이루어져 cold spot이 생성되었으며, 700W에서 2분 조리 후 도달 온도는 약 55oC였다. 특히, 전자레인지 가열 시 내용물의 균일한 가열패턴을 보여주는 hot spot과 cold spot 사이의 온도 상승률은 최대 7,000배까지 차이를 보였다. 따라서 전자레인지 조리 시 내용물의 불균일한 가열로 특정 부위에 열이 집중되는 hot spot의 생성이 포장재 열 변형의 주요 원인으로 판단되었다. 이러한 가열 불균일성은 포장된 내용물에 함유된 당, 염, 수분과 같은 성분들이 점도에 영향을 주는 것에 기인하는 것으로 추측된다. 이러한 점도 변화는 궁극적으로 내용물의 물리적 또는 이온적 특성의 변화를 야기하고, 결과적으로 전자파가 내용물 내부로 침투하는 깊이가 감소되고, 이에 따라 내용물의 유전 특성과 대류 현상에 영향을 주는 것으로 판단된다. 그러나 전자레인지 조리 시 포장재 열 변형 문제의 완전한 해결을 위해서는 향후 포장된 내용물의 세부 성분 별 유전 특성에 대한 보다 세밀한 분석이 필요하며, 아울러 hot spot의 원인이 되는 식품 성분들의 적절한 함량 조절과 함께 포장재, 포장 형태 및 가열 조건 등에 대한 보다 포괄적이고 안정적인 포장시스템 설계와 개발이 필요할 것으로 판단된다. The aim of this study was to investigate the deformation of packaging materials used for ready-to-eat (RTE) foods after the retort process and microwave heating. From the multilayer films consisting of polyethylene terephthalate (PET), polyamide (PA), and cast polypropylene (CPP) in a stand-up pouch form used for RTE foods, some deformation of the CPP layer, which was in direct contact with the food, was observed after the retort process and microwave heating. The damage was more severely caused by microwave heating than by the retort process. This may be attributed to diverse factors including the non-uniform heating in a microwave oven, the sorption of oil into the packaging film, and the different characteristics of food components such as viscosity, salt and water content. The development of heat-resistant packaging materials and systems suitable for microwave heating of RTE foods is required for the safety of consumers.

육가공품 포장재의 안전성 확보를 위한 조사

이근택,윤찬석,박현우,이화신,Lee, Keun Taik,Yoon, Chan Suk,Park, Hyun Woo,Lee, Hwa Shin 한국축산식품학회 2015 축산식품과학과 산업 Vol.4 No.1

회전형 저온 플라즈마 시스템을 이용한 분말식품의 균일한 살균 연구

김명찬,박덕모,한진수,우인봉,김동후,장성은,윤찬석,김인,Kim, Myung Chan,Park, Duck Mo,Han, Jin Soo,Woo, In Bong,Kim, Dong Hoo,Jang, Seong Eun,Yoon, Chan Suk,Kim, In 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.2

In this study, a relatively effective process is used to sterilize Escherichia coli on the surface of micro-sized calcium citrate powder using nitrogen and argon as process gases in a low-temperature vacuum plasma treatment. The purpose of this study is to confirm and to introduce the effectiveness of homogeneous surface treatment for the sterilization of fine inorganic powder by the rotatable low-temperature RF plasma system designed by ourselves. The results of the test using 3M petrifilm showed that there were no remarkable spots in the case of the surface of plasma treated powder, whereas the untreated powder showed many blue spots, which indicating that the E. coli was alive. After 5 days, in the same samples, the blue spots were seen to be larger and darker than before, while the plasma-treated powder showed no changes. The results from FE-SEM analysis showed that the E. coli was damaged and/or destroyed by reactive species generated in the plasma space, resulting in the E. coli being sterilized. Furthermore, the sterilization effects according to the selected parameters ($N_2$ and Ar; flow rate 30 and 50 sccm) adapted in this study were mutually similar, regardless of such different process parameters, and this indicates that homogeneous treatment of powder surfaces could be more effective than conventional methods. Therefore, the plasma apparatus used in this study may be a practical method to use in a powerful sterilization process in powder-type food.

ChondroT의 유전독성 연구

김선길 ( Sun-gil Kim ),김주일 ( Joo Il Kim ),김지훈 ( Ji-hoon Kim ),윤찬석 ( Chan Suk Yoon ),정지원 ( Ji-won Jeong ),나창수 ( Chang-su Na ),김선종 ( Seon-jong Kim ) 한방재활의학과학회 2021 한방재활의학과학회지 Vol.31 No.1

Objectives This study was performed to observe the genotoxic effect of the ChondroT. Methods To evaluate the genotoxicity of ChondroT, an experiment of bacterial reverse mutation test, in vitro mammalian chromosomal aberration test and mammalian erythrocyte micronucleus test in mouse was conducted. Results TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies being greater than 2-fold of the respective negative control value. Both in -S9 mix and +S9 mix, the frequencies of aberration cells with structural aberration and numerical aberrations of chromosome were less than 5%. There was no increase of polychromatic erythrocyte with one or more micronuclei at any dose of test substance compared to the negative control group (p<0.05). Conclusions In TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies was greater than 2-fold of the respective negative control value, showing positive results. ChondroT was considered to be non-clastogenic to Chinese hamster lung (CHL/IU) cells under the present experimental condition. and ChondroT was determined not to induce an increased frequency of micronuclei in the bone marrow cells of male ICR mice under the present experimental condition. (J Korean Med Rehabil 2021;31(1):59-79)

Indomethacin으로 유발된 흰쥐의 위장장애에 ChondroT가 미치는 영향

김주일 ( Joo-il Kim ),김선길 ( Sun-gil Kim ),김지훈 ( Ji-hoon Kim ),윤찬석 ( Chan-suk Yoon ),최지민 ( Ji-min Choi ),최찬헌 ( Chan-hun Choi ),김선종 ( Seon-jong Kim ) 한방재활의학과학회 2020 한방재활의학과학회지 Vol.30 No.3

Objectives The aim of this study was to investigate the inhibitory effect of ChondroT in indomethacin-induced gastric mucosal injury rat model. Methods Sprague-Dawley rats were randomly assigned to intact, control Joins, Celebrex, ChondroT50 and ChondroT200. Indomethacin (25 mg/kg) was used to induce damage to the gastric mucosal injury. ChondroT was administered by orally to inhibit the indomethacin-induced gastric mucosal injury. At the end of the experiment, pH level in stomach, stomach contents volume, tumor necrosis factor-α (TNF- α) level, interleukin-1β (IL-1β) level, prostaglandin E2 (PGE2) level, myeloperoxidase (MPO) activity, erythrocytes, and thrombocytes were measured. Ophthalmologic and histopathological examination was also analyzed. Results pH level in stomach and Stomach contents volume had no difference between Control, PC-Joins, PC-Cele, ChondroT50 and ChondroT200 group. TNF-α level was decreased in PC-Joins, PC-Cele, ChondroT50 and ChondroT200 group and there were no significant difference. IL-1β level was decreased in PC-Joins group and ChondroT200 group compared to control group. PGE2 level had no significant difference between Control, PC-Joins, PC-Cele, ChondroT50 and ChondroT200 group. MPO level and complete blood count level were decreased in PC-Joins, PC-Cele, ChondroT50 and ChondroT200. Symptom score of ophthalmologic examination was decreased in ChondroT50 and ChondroT200 group compared to control group. Conclusion Based on these results, It could be suggested that ChondroT was effective in reducing damage to the gastric mucosal injury. And further study is needed to conduct a rigorous clinical research. (J Korean Med Rehabil 2020;30(3):57-69)