재활용 천연 염료를 이용한 모발의 염색성 시연

윤아람 ( A Ram Yoon ),이주영 ( Ju Young Lee ) 한국미용학회 2010 한국미용학회지 Vol.16 No.1

The purpose of this study is to find the possibility of developing and using recycled natural hairdye as an alternative solution to the hair damage by the use of chemical hairdye and environmental pollution by the remnants of hairdye. First, alum and bean curd were separately added to onion skin extracts as mordant. The L-values(luminosity) of the recycled hairdyes were 49.1 and 43.9, respectively, and 46.6 for chemical hairdye. The a-values (red line) were +14.9, +14.6, and +12.1, respectively, and the b-values (yellow line) were +45.9, +29.3, and +26.3, respectively. Second, when we compare the grape skin extracts to which alum and bean curd were separately added with the chemical hairdye. The L-values were 39.2, 40.3, and 22.5, respectively. The a-values were +10.0, +12.7, and +9.7, respectively, and the b-values were +18.8, +21.8, and +4.9, respectively. Third, we compared the remnants of roasted bean coffee to which alum and bean curd were added with chemical hairdye. The L-value, a-value, and b-value of chemical hairdye were the lowest at 17.1, +2.5, and +0.6. Fourth, we compared Chinese ink to which alum and bean curd were added with chemical hairdye. The L-value, a-value, and b-value of chemical hairdye were the lowest at 16.3, +0.2, and -0.9. Like the results for remnants of roasted bean coffee, the coloring rate of chemical hairdye was high at low L-value. This shows that the chemical hairdye is effective for coloring at low L-value. The results of this study confirmed the possibility of using recycled natural hairdyes for colors with high L-value, and there were no difference between the values of recycled natural hairdyes and chemical hairdye at high luminosity. Therefore, it is believed that these recycled natural hairdyes will be useful as new hairdyes.

일회성 탈진운동과 장기간 지구성 트레이닝이 심장근의 Mn-SOD, HSP70 및 PPAR-γ 단백질 발현에 미치는 영향

전종귀 ( Jong Kui Jun ),윤아람 ( A Ram Yoon ),이광무 ( Kwang Moo Lee ),이왕록 ( Wang Lok Lee ) 한국운동영양학회 2009 Physical Activity and Nutrition (Phys Act Nutr) Vol.13 No.2

아파트 단지 내 고령 친화적 커뮤니티 시설 만족도에 대한 연구

김정현(Kim Jeong-Hyun),윤아람(Yoon A-Ram),하미경(Ha Mi-Kyung) 대한건축학회 2007 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.27 No.1

As apartments become established as a representative housing type, the problems regarding the public nature of apartments are becoming more prominent, and the community within apartment complexes are receiving attention. Also, because Korea's aging population is resulting in the aging residents of apartments, the demand for an apartment service facilities is increasing as well. However, existing apartments' common service facilities have in many cases been designed without considering the elderly, thus making it difficult for them to approach and use the facilities, and expanding beyond that, to contribute to society and form their community. Therefore, the purpose of this paper is to obtain basic data for planning common service facilities in apartments that can respond to the ageing for the apartment constituents, and examine and analyze the current condition of the as their usage and satisfaction level of the elderly residents.

장기간의 지구성트레이닝이 자연발생고혈압쥐의 골격근 Mn-SOD, HSP 70, PPAR- γ 발현에 미치는 영향

전종귀 ( Jong Kui Jun ),윤아람 ( A Ram Yoon ),권순미 ( Soon Mi Kwon ),이영란 ( Young Ran Lee ),이왕록 ( Wang Lok Lee ) 한국운동영양학회 2010 Physical Activity and Nutrition (Phys Act Nutr) Vol.14 No.3

미주신경 절단시 표면활성물질단백 변화에 대한 면역조직학적 연구

전영희(Young-Hee Cheon),배현혜(Hyun-Hae Pae),윤아람(A-Ram Yoon),이지혜(Ji-Hye Lee),백태경(Tai-Kyoung Baik) 대한해부학회 2006 Anatomy & Cell Biology Vol.39 No.5

표면활성물질은 허파꽈리의 표면장력을 낮추어 가스교환을 용이하게 하는 인산지질단백 복합체로써 부족하게 되면 허파꽈리에서의 가스교환에 장애가 초래되어 급성호흡곤란증후군이 유발되는 것으로 알려져 있다. 급성호흡곤란증후군 환자의 허파에서 유리질막 형성, 허파꽈리 중격의 섬유화 및 제2형 허파꽈리세포내 표면활성물질의 감소 등과 같은 변화가 초래됨은 이미 잘 알려져 있으나 두부손상으로 인해 유발되는 급성호흡곤란증후군의 정확한 발병기전에 대한 연구는 보고된 바가 매우 드물다. 그러므로 연구자는 두부손상 혹은 신경인성 급성호흡곤란증후군의 병리학적 기전에서 미주신경의 역할을 규명하고자 하위 경부 미주신경을 절단한 후 허파꽈리세포에서 표면활성물질단백의 변동을 조사하였다. 체중 230 gm 내외의 Spraque-Dawley 흰쥐를 사용하였고 정상대조군 및 미주신경 절단군으로 구분하여 수술 후 3시간, 8시간 및 24시간 경과후에 허파를 절취하였다. 허파조직은 Hematoxylin-Eosin 염색, Masson’s trichrome 염색, SP-A 및 ProSP-C에 대한 면역조직화학염색을 시행하여 다음과 같은 결과를 얻었다. 1. 미주신경절단군에서는 허파꽈리 상피세포의 괴사 및 두꺼운 유리질막 형성으로 허파꽈리의 형태가 변형되었고 비후된 허파꽈리 중격에서 다수의 염증세포 침윤과 아교원섬유가 증가되었으며 허파꽈리 부종이 관찰되었다. 2. 미주신경절단군의 허파조직 내 SP-A 및 ProSP-C 면역활성은 수술 후 시간이 경과됨에 따라 감소되었다. 이상의 결과를 종합하면, 하위 경부 미주신경 절단으로 인한 자율신경실조가 SP-A 및 SP-C와 같은 표면활성 물질단백의 대사에 변동을 초래하고, 허파조직에 속발성 염증반응을 유발하여 급성호흡곤란증후군과 유사한 급성 허파 손상을 유도하는 것으로 생각된다. Pulmonary surfactant prevents alveolar collapse by reducing alveolar surface tension and aids gaseous exchange in the lung. Since inadequate production of pulmonary surfactant is a key etiological process in ARDS, surfactant may play an important role in pathogenesis of ARDS. To provide a clue for establishing pathological mechanism of post-traumatic or neurogenic ARDS, we studied the influence of the vagal innervation on pulmonary surfactant metabolism. A total of 20 S-D rats (about 230 gm wt. each) were divided into two conditions: normal control and vagotomized groups. The vagotomized rats were subdivided into 3 hours, 8 hours and 24 hours groups. To preserve the superior cervical cardiac branches, both vagus nerves were cut at the lowest part of the carotid triangle. Cannula for adequate respiration and suction was fitted into the trachea. The lung tissue were processed for H&E, Masson’s trichrome, Immunohistochemistry using anti-surfactant protein A (SP-A) and .anti-prosurfactant protein C (ProSP-C). The results were as follows; 1. The lungs of the vagotomized rats showed alveolar edema, fibrosis with infiltration of inflammatory cells and hyaline membrane formation. 2. In the lungs of the vagotomized rats, SP-A and ProSP-C immunoreactivity was decreased in proportion to postoperation time. Consequently, it can be postulated that autonomic disturbances caused by vagal interruption may induce ARDS-like pulmonary damage by modulating alveolar surfactant protein metabolism and by evoking the secondary inflammatory processes.

국소적 대뇌허혈 동물모델에서 Diazoxided의 신경보호효과

전영희(Young-Hee Cheon),서병현(Byeong-Hyun Suh),윤아람(A-Ram Yoon),이지혜(Ji-Hye Lee),우란숙(Ran-Sook Woo),송대용(Dae-Yong Song),백태경(Tai-Kyoung Baik) 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.4

허혈전조건화는 장시간의 허혈 발생 전에 일시적이고 반복적인 허혈을 유도하여 대뇌 손상을 감소시키는 것으로, 사립체의 K?ATP channel opener가 허혈전조건화를 유도하여 허혈세포에서 보호효과를 나타내는 것으로 보고되고 있으나 아직 자세한 기전은 알려져 있지 않다. 이 연구에서는 국소적 대뇌허혈 동물모델에서 허혈 손상시 K?ATP channel opener인 diazoxide 전처치에 의한 신경세포 보호효과의 기전을 밝히고자 하였다. 실험동물로는 300~350g 내외의 Sprague-Dawley계 흰쥐(n=34)를 사용하였으며, 정상대조군과 2시간 허혈군, 24시간 허혈군, diazoxide 전처치후 2시간 허혈군과 diazoxide 전처후 24시간 허혈군으로 세분하였다. 변형된 Longa법을 이용하여 국소적 뇌허혈술을 실시하였으며, diazoxide는 뇌허혈술 15분전에 3mg/kg을 넙다리동맥으로 투여하였다. 중간대뇌동맥 폐쇄에 의해 동측 대뇌겉질 대부분과 줄무늬체에서 뇌경색이 초래되었으며, diazoxide 전처치군에서는 허혈군에 비하여 경색부피가 감소되었다. 동시에 diazoxide 전처치군에서는 경색주변조직에서의 해면체성변형 및 신경세포의 퇴행성변화가 감소하는 소견을 보였다. 한편 2시간 허혈군에서는 정상대조군과 동일하게 TUNEL 염색에 양성반응을 보이는 세포를 관찰할 수 없었으나, 24시간 경과군에서는 다수의 신경세포가 양성반응을 보였다. 허혈군 및 diazoxide 전처치군 모두에서 허혈 2시간째 c-Fos에 면역양성반응을 보이는 신경세포의 수가 크게 증가하였으나, 24시간 경과군에서는 정상대조군 수준으로 다시 감소하였다. 한편 Bcl-2 혹은 pAkt에 면역양성반응으로 보이는 신경세포의 수는 허혈 2시간째부터 허혈대조군에 비해 통계적으로 유의하게 높게 나타났으며, 이러한 현상은 허혈 24시간째까지 지속되었다. 이상의 결과를 토대로, K?ATP channel opener인 diazoxide의 전처치는 허혈 초기 단계부터 세포자멸사 억제단백인 Bcl-2나 pAkt의 활성을 증가시키므로써 신경세포를 보호하고 세포 손상을 지연시킬 수 있을 것으로 여겨진다. Ischemic preconditioning is the earlier stress adaptive response that occurs during repeated episodes of the brief ischemia and reperfusion. It is now well known that this adaptive response can render the neuron more tolerant to the subsequent potential lethal ischemic injury. Although the selective mitochondrial K?ATP channel opener induces protective effects similar to that of ischemic preconditioning, the underlying mechanism is not konown yet. The purpose of this study was to investigate the mechanism of neuroprotective effect of diazoxide, a mitochondrial K?ATP channel opener, pretreatment on a focal cerebral ischemic injury of rat brain. Thirthy-four Sprague-Dawley rats were used. Animals were randomly divided into normal control group, middle cerebral artery (MCA) permanent occulusion group(experimental control group), and diazoxide pretreated group. Animals were sacrified at 2 hours or 24 hours after MCA occulusion injury. For inducing the focal cerebral ischemic injury, the left MCA was occuluded by modified Longa's method. Diazoxide (3mg/kg) was administrated though the femoral artery at 15 minutes earlier to surgical procedures. TTC-stained brain sections of experimental group showed a remarkable infarct injury in the ipsilateral cerebral cortex and striatum. However, the infarction volume of the diazoxide pretreated group was significantly reduced. Accordingly, the number of neurons undergoing eosinophilic degeneration and unclear chromatin condensation was reduced by diazoxide pretreatment. TUNEL-positive neurons were not detected at 2 hours after MCA permanent occlusion but lots of them were observed at 24 hours. The number of c-fos immunoreactive neurons was remarkably increased at 2 hours following MCA permanent occulusion and reduced to the basal level at 24 hours in both experimental control and diazoxide pretreated group. However, the number of Bcl-2 or pAkt immunoreactivitive neurons of the diazoxide pretreated group outnumbered those of the experimental control group at all timepoints in our experiment. In conclusion, the pretreatment of diazoxide, K? channel opener, could have neuroprtective effects on ischemic neurons by upregulating the expression of anti-apoptotic proteins, like Bcl-2 or pAkt.

장기간 중강도 운동훈련과 일회 탈진운동이 쥐 비장세포의 싸이토카인 생성량 및 증식반응에 미치는 영향

이왕록 ( Wang Lok Lee ),박희근 ( Hee Geun Park ),전종귀 ( Jong Kui Jun ),정준현 ( Jun Hyun Jeong ),윤아람 ( A Ram Yoon ),권순미 ( Soon Mi Kwon ),김명화 ( Myung Hwa Kim ) 한국운동생리학회 2009 운동과학 Vol.18 No.4