Minocycline 및 $TGF-{\beta}1$이 배양 인체 치은섬유모세포와 치주인대세포에 미치는 영향

윤동환,유형근,신형식,Yoon, Dong-Hwan,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.1

One of the initial events required for periodontal regeneration is the attachment, spreading and proliferation of fibroblasts at the healing sites. These have been reported that minocycline stimulates the attachment of gingival fibroblasts and periodontal ligament cells and $TGF-{\beta}1$ enhances the proliferation of periodontal ligament cells. The purpose of this study was to evaluate and confirm the effect of minocycline and $TGF-{\beta}1$ on human gingival fibroblasts and periodontal ligament cells. That gingival fibroblasts and periodontal ligament cells used in this study were obtained from the explants of healthy periodontal ligaments and gingival tissues of extracted 3rd molars or premolar teeth extracted from the patients with orthodontic treatment. The cells were cultured in ${\alpha}-MEM$(minimal essential medium) supplemented with antibiotics and FBS(fetal bovine serum) at $37^{\circ}C$ in a humidified atmosphere of 5% carbon dioxide-95% air. Cells were used between the 5th to 8th passage in this study. The attachment and activity of both cells were evaluated by MTT assay. The results were as follows: 1. Maximum gingival fibroblast attachment was seen at a $50{\mu}g/ml$ dose of minocycline, while maximum periodontal ligament cell attachment was seen at a $100{\mu}g/ml$, and exposure of both cells to minocycline above maximal attachment dose results in a decline from maximum attachment. 2. The activity values of both cells tested minocycline were below to the control activity values at all concentrations. 3. The attachment values of both cells tested $TGF-{\beta}1$ were below or similar to control attachment values. On the above the findings, minocycline stimulated the cell attachment of gingival fibroblasts and periodontal ligament cells and $TGF-{\beta}1$ enhances the cell activity of periodontal ligament cells.

VaRTM과 VAP 공정의 수지 충진실험 및 해석에 관한 연구

윤동환,서경호,권유정,최진호,Dong-Hwan Yoon,Kyeong-Ho Seo,Yu-Jung Kwon,Jin-Ho Choi 한국복합재료학회 2023 Composites research Vol.36 No.5

VaRTM(Vacuum assisted resin transfer molding) and VAP(Vacuum assisted process) processes are a type of RTM(Resin transfer molding) process, and are typical out-of-autoclave (OOA) processes that can manufacture large structures at low cost. In this paper, a resin filling test was conducted to compare the VaRTM and VAP processes, and the filling process and dimensional stability were compared. In addition, an analysis method to simulate the filling process was developed, and a dielectric sensor was used to detect the flow front of the resin, which was compared with the analysis results. From the resin filling test, the total filling time of the composite plate was measured to be 48 minutes for the VAP process and 145 minutes for the VaRTM process, and the filling time by the VAP process was reduced by about 67%. In addition, it was confirmed that the VAP process was superior to the VaRTM process in the thickness control ability and uniformity of the composite plate.

Simultaneous observations of SiO and $H_2O$ masers toward AGB and post-AGB stars

윤동환,조세형,김재헌,조치영,윤영주,박용선,Yoon, Dong-Hwan,Cho, Se-Hyung,Kim, Jaeheon,Cho, Chi-Young,Yun, Youngjoo,Park, Yong-Sun 한국천문학회 2012 天文學會報 Vol.37 No.2

We performed simultaneous observations of SiO v=1, 2, $^{29}SiO$ v=0, J=1-0 and $H_2O$ $6_{16}-5_{23}$ maser lines toward 132 AGB and 183 post-AGB stars in order to investigate how evolutionary characteristics from AGB to post-AGB stars appear in these two maser emissions. The observations were carried out from 2011 February to 2012 March using the Korean VLBI Network 21-m radio telescopes. We have detected SiO and/or $H_2O$ maser emission from 29 sources out of 183 post-AGB stars including 19 new detections. Of 132 AGB stars which are mainly selected based on the IRAS Point Source Catalog, we detected SiO and/or $H_2O$ maser emission from 38 stars including 18 newly detected sources. An evolutionary characteristic from AGB to post-AGB stars is discussed in IRAS two-color diagram. It is found that SiO v=2, J=1-0 maser emission without SiO v=1 maser detections was detected from 8 sources among 21 SiO detected post-AGB stars and the intensity of SiO v=2, J=1-0 maser tends to be much stronger than that of SiO v=1. We also found that for the post-AGB stars the maser detection rate of blue group sources (which have higher outflow velocities than red group) are higher than that of red group. Especially, only $H_2O$ maser emission was detected from 7 sources among 94 red group sources without SiO maser detections.

홍화씨 추출물이 조골모유사세포활성 및 골재생에 미치는 영향

윤동환,이승철,김명은,김은철,유형근,김윤철,신형식,Yoon, Dong-Hwan,Lee, Seoung-Cheul,Kim, Myung-Eun,Kim, Eun-Cheol,You, Hyung-Keun,Kim, Youn-Chul,Shin, Hyung-Shik 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.4

The purpose of the present study is to examine the effect of cell proliferation and alkaline phosphatase activity in osteoblastic cells and to compare the bone healing ability of rat calvarial defects between the control group and the safflower seed extract treated group. Osteoblastic cells were obtained from calvariae of a fetal rat. Cells were cultured containing DMEM and safflower seed extract ($10^{-6}g/ml$, $10^{-3}g/ml$) at $37^{\circ}$ with 5% $CO_2$ in 100% humidity for 3 days. MTT was performed to examine the viability of the cells, and alkaline phosphatase activity was analyzed to examine the mineralization in vitro. Rat calvarial defects($5{\times}5mm$) in 250g Sprague-Dawly were made using round bur. Rats were administrated with safflower seed extract(0.35g/kg/day) for experimental periods. Calvarial defects were studied histopathologically and immunohistochemically at 1,4, and 8 weeks. High concentration group($10^{-3}g/ml$) of safflower seed extract significantly increased in the cell proliferation and alkaline phos phatase synthesis in osteoblastic cells. The infiltration of inflammatory cells and osteoclastic activities were decreased in the safflower seed extract treated group as compared with control group. Bone maturation was accelerated in the safflower seed extract treated group as compared to control group. No difference in osteoinductive process was observed between the control and the safflower seed extract treated group. Immunohistochemical observation revealed that protein expression of TGF-$\beta$and osteonectin during early healing phase in the safflower seed extract treated group was slightly increased as compared to control group. These results indicate that safflower seed extract promotes the healing process in bony defect of rat calvariae, and retains a potential applicability as an adjuvant therapeutic modality for regeneration of periodontal bony defect.

항공 우주 해상 : DO-278의 Validation & Verification에 적합한 WA-DGNSS 기준국 소프트웨어의 모듈별 통합 검증 방법론 제시

윤동환 ( Dong Hwan Yoon ),박병운 ( Byung Woon Park ),최완식 ( Wan Sik Choi ),기창돈 ( Chang Don Kee ),서승우 ( Seung Woo Seo ),박준표 ( Jun Pyo Park ) 한국항행학회 2015 韓國航行學會論文誌 Vol.19 No.1

WA-DGNSS는 지상에서 수신한 GNSS 신호를 관련 오차 계산 후 보정 정보를 생성하여 위성을 통해 사용자에게 보정 정보를 제공하는 시스템을 말한다. 사용자는 이 시스템을 통해 위치 정확도 향상 및 GNSS 신호에 대한 신뢰성을 보장 받는다. 또한 국제민간항공기구(ICAO)에서는 항공기 이착륙 절차에 광역 보정시스템의 적용을 권고하고 있다. 본 논문에는 항공관련 소프트웨어개발 절차 관련 규격문서인 RTCA DO-278의 소프트웨어 검증 프로세스를 참고하여 기 구축된 WA-DGNSS 광역 기준국 소프트웨어의 모듈 및 통합 테스트 단계를 구성하여 검증을 위한 방법론을 제시한다. 또한 제시한 방법론을 통해 기준국 소프트웨어 테스트를 통계적으로 검증하였으며 이러한 검증을 통해 기준국 소프트웨어의 기능이 적절히 수행됨이 확인되었다. WA-DGNSS is a system to service for users using a satellite which received correction data from ground station that calculates the relative errors of the tracked GNSS signals and sends to a satellite. Users are guaranteed the reliability of the GNSS signal and the accuracy of positioning. ICAO recommends the application of WA-DGNSS for the airplane taking off and landing process. In this paper, we suggests methods to verify of the pre-developed WA-DGNSS reference software constituting modules and an integration test process refer to the RTCA DO-278 which is a document for the development process of an aeronautics software. Also, we statistically verified the reference software test through our methods. And then, we confirmed to performance the function of the reference software properly.

골관절염과 TGF-B1 유전자의 T869C 단일염기다형성과의 상관관계

윤동환 ( Dong Hwan Yun ),김현정 ( Hyun Jung Kim ),김희상 ( Hee Sang Kim ),안경회 ( Kyung Hoi Ahn ),강준구 ( Jun Goo Kang ),하은영 ( Eun Young Ha ),백형환 ( Hyung Hwan Baik ),윤경식 ( Kyung Sik Yoon ) 경희대학교 경희의료원 2006 慶熙醫學 Vol.22 No.2

IPA를 활용한 팬데믹 환경에서 대학생 여가활동에 관한 연구

윤동환(Dong-Hwan Yoon),한승훈(Seung-Hoon Han) 한국관광연구학회 2022 관광연구저널 Vol.36 No.1

울릉도에서 도래된 삼척의 오분동 당신화

윤동환 ( Dong Hwan Yoon ),이승철 ( Seung Cheol Lee ) 한국도서학회 2012 韓國島嶼硏究 Vol.24 No.4

From the shrine myth of Ulleungdo and Ovoondong in Samcheok, this research covers the culture exchange between island and land. The original shrine myth related to the Ovoondong shrine is now slowly being erased from the village people`s memories. Today, the village people only enshrine three godships in their shrine. They are the gods of shrine, sun and land. The gods of sky and mountain, which are viewed as godships of the present, are displayed as a ceremonial table type of Samcheok Hyanggyo. The shrine myth of Ovoondong is slowly changing due to people`s imperfect memories and outer influence from the press and local government. The myth is now recreated by the transmission agents and external factors. Shrine myth was once the symbol of folk belief, that maintained and reinforced the village order. Also it helped promote the village ceremony, and there was a rule that the representative or ceremony director of the village should lead the village people who participated in the village ceremony. In short, the Shrine myth played a key role in linking faith and order of the village. In the case of the Shrine myth of Ovoondong in Samcheok a strange stone story was added to the existing shrine. Meanwhile the myth of Tae-ha dong in Ulleungdo was related to the death of man and woman, which originated from a land shrine myth. Since myth is a historical product, it shows the lifestyle and thoughts of people from different periods and represents the villager`s life and history. Thus, it should be re-evaluated and introspected from a modern point of view. From this research it can be concluded that when culture is interpreted upon the absence of historical facts, many errors may occur.

MODE I DCB test의 I-fiber stitching 각도에 따른 영향성 연구

윤동환(Dong-Hwan Yoon),박규영(Gyu-Yeong Park),안우진(Woo-Jin An),최진호(Jin-Ho Choi) 한국항공우주학회 2020 한국항공우주학회 학술발표회 논문집 Vol.2020 No.11

관측 행렬 산출 기법 별 DGNSS-CP 성능 비교

신동현 ( Dong-hyun Shin ),임철순 ( Cheol-soon Lim ),석효정 ( Hyo-jeong Seok ),윤동환 ( Dong-hwan Yoon ),박병운 ( Byungwoon Park ) 한국항행학회 2016 韓國航行學會論文誌 Vol.20 No.5

저가형 GNSS (global navigation satellite system) 수신 모듈에 DGNSS (differential GNSS) 서비스를 적용하기 위한 방안으로 거리 영역의 보정정보를 위치 영역으로 투영한 후, stand-alone으로 산출한 위치에 적용하는 DGNSS-CP 방식이 제안된 바 있다. DGNSS-CP 를 상용 수신기 또는 휴대폰에 적용하기 위해서는 항법 방정식의 관측행렬을 이용하여 위치영역 투영 방정식을 구성하므로, 각 위성의 시선벡터를 산출하여야 한다. GNSS 항법 메시지, 배치 정보 등이 시선벡터 산출을 위하여 사용되는데, 각 방법에 따라 정확도와 연산량 등의 성능에 차이가 발생한다. 본 연구에서는 제시된 두 가지 시선벡터 산출 방식에 따라 DGNSS-CP 의 성능에 어떠한 영향을 끼치는지 확인하기 위하여, Septentrio PolaRx4 Pro 수신기에서 stand-alone mode 로 저장된 데이터에 해당 알고리즘을 적용하였고, 배치 정보를 사용하는 방법이 궤도정보를 사용하는 방법에 비해 정확도 면에서는 그 성능이 RMS (root mean square) 0.1 m 가량 저하되는 반면, 연산량은 약 1/15수준으로 줄일 수 있음을 확인하였다. Several low-cost global navigation satellite system (GNSS) receivers do not support general range-domain correction, and DGNSS-CP (differential GNSS) method had been suggested to solve this problem. It improves its position accuracy by projecting range-domain corrections to the position-domain and then differentiating the stand-alone position by the projected correction. To project the range-domain correction, line-of-sight vectors from the receiver to each satellite should be calculated. The line-of-sight vectors can be obtained from GNSS broadcast ephemeris data or satellite direction information, and this paper shows positioning performance for the two methods. Stand-alone positioning result provided from Septentrio PolaRx4 Pro receiver was used to show the difference. The satellite direction information can reduce the computing load for the DGNSS-CP by 1/15, even though its root mean square(RMS) of position error is bigger than that of ephemeris data by 0.1m.