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RISS 인기검색어
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- 저자 : 유탁(Tak You) (검색결과 2 건)
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한범구(Beom Ku Han),이우진(Woo Jin Lee),유탁(Tak You),박인호(In Ho Park),조도현(Do Hyun Jo) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.6
The survey on the chitinolytic activity of some plants was performed for the purpose of obtaining some reliable and inexpensive sources of chitinase. Rice, soybean for sprouting, kiwi fruit, almond and crude papain were investigated. Rice bran, seed coat of the soybean and the pericarp of kiwi fruit showed a considerable activity, while the bean after the removal of the seed coat, the mixture of rice integument and endosperm, polished rice, and defatted soybean powder didn`t have any detectable activity. These crude enzymes have shown to contain both endo- and exochitinase activity. The effects of pH and temperature on the enzyme activity were variable. Furthermore we have observed the chitosanolytic activity from these enzyme preparations. The rice bran had the highest activity in the enzymatic degradation of chitosan, and seed coat of soybean and the pericarp of kiwi fruit followed. On the basis of the fact that crude papain was not only commercially available but also the most potent in the endochitinase activity and the lowest in the exochitinase activity, we could conclude that crude papain was considered as the most suitable source of the chitinase among plants studied in this paper. In addition, rice bran was worth further investigation from the point of utilizing agricultural by-product.
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한범구,유탁,이우진,조성준,박인호,조도현 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.3
Crude chitinolytic enzymes were prepared from the chyme, the mucous lining and the remaining tissue of the gizzard, and some enzymatic properties were investigated. Freezing and thawing of the sample did not affect the activity of the subsequent crude preparations of the enzyme. The optima: incubation time and temperature were 2 hours and 10'C, respectively. The pH optima and thermostability were variable, depending upon the source of the enzyme and the type of enzyme action. Under experimental conditions 165mg of N-acetylchitooligomers could be obtained from a gizzard after an incubation time of 18 hours.
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