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다제내성 Staphylococcus aureus 에 항균활성을 나타내는 CNU30122 균주가 생산하는 항생물질
윤봉식(Bong Sik Yun),조수묵(Soo Muk Cho),김창진(Chang Jin Kim),유익동(Ick Dong Yoo) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6
During the screening for the antimicrobial agents against multidrug-resistant Staphylococcus aureus, we isolated an active compound produced by strain CNU30122. The active compound was purified from culture broth by HP-20 column chromatography, ethylacetate extraction. silica gel column and Sephadex LH-20 column chromatographies and HPLC. Based on various NMR studies including ¹H-¹H COSY. ¹H-^(13)C COSY and HMBC experiments. the active compound was identified as fusidic acid.
조수묵,서건식,유승헌,유익동,신관철 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.6
우리나라에서 재배되고 있는 영지버섯(적지)과 형태적으로 다른 백색변이주의 자실체를 수집하여 형태적인 특성을 조사하고 자실체로부터 순수분리한 균사체의 형태 및 최적배양조건을 조사하였다. 그 결과 영지버섯 백색변이주의 자실체 형태는 백색으로 대가 없고 포자는 타원형 이었으며 균사는 정상주에 비하여 가늘었다. 영지버섯 백색변이주의 최적 생육배지는 potato sucrose agar 배지이었으며 균사생육 최적 pH는 5.5이었다. 영양원으로 탄소원은 D-sorbitol과 carboxyl methyl cellulose를, 질소원으로는 무기태 질소인 Ca(NO_2)_2를, 비타민류는 nicotinic acid를 가장 잘 이용하였으며, 무기염류간에는 큰 차이가 없었다. A morphologically different form of Ganoderma lucidum was isolated from a cultivator’s farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white whereas those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5. The mutant preferred carboxyl methyl cellulose and calcium nitrate [Ca(NO_2)_2] as a carbon and nitrogen sources, respectively. Nicotinic acid was a favorable vitamin source for growth of the mutant. No significant differences were observed in utilization of inorganic salts.
조수묵,서건식,유익동,신관철 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.2
영지버섯 백색변이주인 Ganoderma lucidum G4142 균주의 균사생장 및 비자실체성 담자포자 형성에 미치는 광 및 온도의 영향을 밝히기 위하여 광도(light intensity), 광질(light quality), 광 조사시간 및 온도의 영향을 조사하였다. Ganoderma lucidum G4142 균주는 광 조사에 의해 균사생장이 현저하게 억제되었으나, 반면 비자실체성 담자포자의 생성은 오히려 풍부하게 형성되었다. 영지버섯 백색변이주의 비자실체성 담자포자 형성은 주기적인 light 16시간, dark 8시간의 광조사 조건하에서 1000 lux의 백색광을 조사하였을 때 가장 양호하였고, 450∼620 nm의 청색광과 황색광은 백색광 조사보다 많은 비자실체성 담자포자를 형성하였다. 그러나 16일동안 배양하여도 자실체 원기(pinhead) 및 후막포자(chlamydospore)는 형성되지 않았다. 한편 생육최적온도는 30℃이었으며, stroma의 형성시기는 Ganoderma lucidum G4142 균주는 배양 5일 후, 정상주인 Ganoderma lucidum G4086 균주는 배양 10일 후부터 stroma가 형성되었다. White mutant of Ganoderma lucidum(G4142) induced the non-basidiocarpous basidiospores(NBB) from the aerial mycelia on agar media by the light illumination. Light was found to be necessary for NBB formation, but it also inhibited the growth of mycelium. The best sporulation was obtained at the periodic exposure of 16 hour light and 8 hour dark. Blue and yellow light were the most effective on sporulation, however, near UV and red light did not induce any spores. Effective light intensity for NBB bearing was about 1,000 lux as white light. Even after 16 days of culture, this strain did not form the pinhead nor chlamydospore. Optimum temperature for the mycelial growth and NBB formation were 30℃. Ganoderma lucidum G4142 exhibited the formation of stroma after five days of incubation at 30℃.
신령버섯 (Agaricus blazei) 으로부터 면역증강활성 다당류의 분리 및 화학적 특성
김환묵,유익동,조수묵,박정식,김광포 한국균학회 1999 韓國菌學會誌 Vol.27 No.2
Water-soluble polysaccharides from the fruiting bodies of Agaricus blazei Murill were extracted with 0.9% sodium chloride and hot water, successively. The purified polysaccharides showed a potent immunostimulating activity. Eight major polysaccharides, which were named from AG-1 to AG-8, were fractionated and purified by ethanol precipitation, ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. These polysaccharides were identified to be homogeneous by analysis of HPLC. Three major active polysaccharides (AG-2, -3, and -6) showed relatively strong immunostimulating activity. AG-2 and -3 were composed of glucose, galactose and mannose in the molar ratios of 74.0:15.3:10.7 and 63.6:17.6:12.7, respectively. AG-6 was composed of glucose and ribose in the molar ratios of 81.4:12.6.