원저 : 개에서 Irrigation / Aspiration 장비와 점탄물질이 수정체 낭외적출술후의 합병증에 미치는 효과

우흥명,권오경,남치주 (Heung Myong Woo,Oh Kyeong Kweon and Tchi Chou Nam) 한국임상수의학회 1997 한국임상수의학회지 Vol.14 No.1

The Role of nerve Growth Factor on Corneal Wound Healing in Dogs

우흥명 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4

각막상피의 창상치유에 중요한 역할을 하는 것으로 알려진 Nerve Growth Factor(NGF)의 내인성 변화와 국소적 투여효과를 알아보았다. 6마리의 Beagle 을 사용하여 비교군인 오른쪽 눈에만 6mm의 기계적인 각막 창상을 만들었고 왼쪽 눈은 대조군으로 사용하였다. 눈물은 수술후 1주일동안 매일, 각막 상피는 수술중 그리고 수술후 7일에 각각 채취하여 NGF 농도를 enzyme-linked immunosorbent assay (ELISA)를 사용하여 측정하였다. 2차 실험으로, 16마리의 Bealgle을 사용하여 오른쪽 눈에 위와 동일한 창상을 만든 후, 창상 부위가 회복될 때까지 recombinant human (rh) NGF (n=4), murine NGF (n=6) 그리고 anti-NGF blocking antibody (n=6)를 6시간마다 점안하였으며, 왼쪽 눈은 bovine serum albumin (BSA)를 점안하여 대조군으로 사용하였다. 창상 면적은 NIH image software를 이용하여 분석하였다. 대조군에 비하여, 비교군의 눈물내 NGF는 치유초기에 현저하게 증가하였으며, 각막 상피의 NGF도 유의적인 증가를 보였다. 그러나 rh NGF, murine NGF, anti-NGF blocking antibody처치군과 BSA처치군 간에는 각막창상 치유에 유의적인 차이가 인정되지 않았다. 각막창상후, rh NGF 혹은 murine NGF의 추가적인 국소점안은 정상개의 각막상피 치유에 효과가 없었으나, 창상후 초기 치유기동안 눈물과 손상받은 각막상피에서 내인성 NGF의 급격한 보상성 증가로 미루어 NGF는 각막 상피의 창상치유에 중요한 역할을 하는 것으로 사료된다. To investigate the modulation of nerve growth factor (NGF) during corneal epithelial wound healing and the effect of topical NGF on corneal epithelial wound healing in dogs. An axial epithelial defect was created in the right eye using 6mm axial corneal mechanical debridement while the left served as an unwounded control. The tears were collected from both eyes during 1 week and the corneal epithelium was processed for the measurement of NGF at day 0 and 7. The NGF content of tears and corneal epithelium was determined by enzyme-linked immunosorbent assay. In another experiment, the animals were divided into 3 groups. The right eyes in each group were treated every six hours with 200 ug/ml of recombinant human (rh) NGF, murine NGF, or 600 ug/ml of anti-NGF blocking antibody. The left eye of each animal was treated with bovine serum albumin (BSA) to serve as controls. Wound healing was analyzed using NIH image software. Tear NGF was markedly increased in the wounded eyes, relative to tears from control eyes during the early healing period. The NGF content of the corneal epithelium was elevated in the wounded eye (p=0.024). Time to wound closure and rate of epithelial migration were not significantly different between the NGF treated or the NGF antibody treated, and the control BSA treated eyes. Corneal epithelial wounding increased NGF content only on the wounded side during the early healing period. Neither topical recombinant human or murine NGF affected corneal epithelial wound healing in the normal dog.

개의 Intraocular Lymphoma

우흥명 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4

Lymphoma is one of the most common malignant neoplasms of the dog. Little has been published on intraocular lymphoma in dogs. This report presents a 8-year-old neutered male mixed dog with a previous history on inflammatory debris on the anterior and posterior lens capsules, vitreal hemorrhage, generalized peri-pheral lymphadenopathy and a palpable mid-abodominal mass. History, clinical signs, radiography, cytology, histopathology and chemotherapeutic response confirmed multicentric lymphoma with secondary ocular infla-mmation. Intraocular lymphoma, the most common secondary neoplasm of the canine eye, may be the present-ing evidence of systemic disease. This report describes a dog that presented with ocular manifestations of multicentric lymphoma. 림프종은 임상에서 많이 발생되는 개의 악성 종양이지만, 눈 안에서의 발생은 그 예가 드물다. 본 연구는, 수정체낭의 혼탁, 초자체 출혈, 전신적인 임파선증 및 복부중앙의 응괴가 관찰되는 8년령 잡종견에서 병력, 임상증상, 방사선 검사, 세포 및 조직병리학 검사, 화학요법에 의한 반응을 통하여 속발성 안내 염증을 동반하는 전신적인 다발성 lymphoma를 확진하는 방법을 알아보았다. 개의 눈 안에서 관찰되는 속발성 lymphoma는 전신적인 lymphoma를 예견하는 중요한 임상소견이 될 수 있으므로 수의 임상에 응용이 가능하리라 판단된다.

개의 뇌사와 신장이식

우흥명,권오경 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4

Brain dead (BD) patients remain the largest source of solid organs for transplantation. BD has shown to decrease graft function and survival in rodent models. The aim of this study was to evaluate how brain death affects graft viability in the donor and kidney tolerance to cold preservation as assessed by survival in a canine transplantation. 13 Beagle dogs were used for the study. Brain death was induced by the sudden inflation of a subdural balloon catheter with continuous monitoring of arterial blood pressure and eletroencephalographic activity (n=3). Sixteen hours after conformation of brain death, kidney graft were retrieved (n=6). Non-BD donors served as controls (n=4). All kidneys were flushed with University of Wisconsin (UW) solution and preserved for 24 hours at 4$^{\circ}C$ before transplantation. Recipient survival rates, serum creatinine level were analyzed. Brain death induced the well-known Cushing reaction with a severe increase in blood pressure and tachycardia. Thereafter, cardiac function returned progressively to baseline within 8 hours and remained stable until the end of the experiment. All of dogs in both group transplanted were survived until 7 days (100%), and the kidneys showed functional early rejection at 8.3$\pm$0.5 days and 8.5$\pm$0.5 days after transplantation, in BD and allograft group, respectively. BD kidneys were functionally similar to control kidneys for 7 days after transplantated. Brain death has no deleterious effect on preservation injury and survival of dog kidney transplantation, although it induces changes in hemodynamic parameters. This study reveals that kidneys from BD donors do not exhibit more ischemia reperfusion injury, and support good early function and survival.

변형된 안검봉합술

우흥명 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.1

Tarsorrhaphy is widely used to protect the cornea, reduce evaporation of tears, and facilitate reepithelialization of the ocular surface. However, a dilemma ensues in patients when frequent neurological evaluations and postoperative examinations are needed. The modified temporary tarsorrhaphy in this study was simple to perform, provided stable eyelid closure, and could be opened and closed as needed. It could be easily reversed with minimal complications of the eyelid and it allowed the surgeon to control traction of the bolsters in all of 45 dogs with corneal epithelial defects. Also, The globe could be examined without further anesthesia, sutures, and pain at any time after surgery. It was concluded that this modified method might be effective for evaluation of the corneal healing process and useful for ocular surface reconstruction clinically.

개의 건성 각결막염과 수술적 처치

우흥명 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4

건성 각결막염(KCS)은 임상에서 많이 접하게 되는 개의 안과 질환이다. 눈물샘으로부터 수성층의 눈물생산이 억제되거나 정지되어 각막 표면 눈물층의 양적변화와 함께 KCS가 유도된다. 본 연구는 KCS로 잠정진단을 받아, 본 대학병원에 의뢰된 개를 대상으로 조직 병리학적 변화를 검사하고, KCS의 진단 및 내과적 치료 방법들을 검토하였으며, 내과적 치료에 반응하지 않은 예를 외과적 처치인 parotid duct transposition(PDT)으로 치료하는 방법을 서술하고자 한다. Keratoconjunctivitis sicca (KCS) is a relatively common eye disease in the dog, Reduction or arrest of aqueous tear production by lacrimal glands results in quantitative alteration of the precorneal tear film (PTF) and KCS may result. A dog with a presumptive diagnosis of KCS referred was used for the study of KCS. Cytological and histopathologic examination is performed, and the various types of medical and surgical treatment are studied, including the operation of parotid duct transposition (PDT). This report described unilateral KCS in a dog of unknown etiology who responded to PDT.

가토에서 엑시머레이저 치료광각막절제술 후 양막이 창상치유에 미치는 효과

우흥명(Heung Myong Woo),김만수(Man Soo Kim),권오경(Oh Kyeong Kweon) 대한안과학회 1999 대한안과학회지 Vol.40 No.7

가토에서 각막혼탁의 억제에 대한 양막이식의 효과

우흥명(Heung Myong Woo),김만수(Man Soo Kim),권오경(Oh Kyeong Kweon) 대한안과학회 1999 대한안과학회지 Vol.40 No.9

Development of bio-artificial liver using porcine soild organ

Heung-Myong Woo(우흥명) 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7

개의 기관에 적용한 변형제작한 기관외보철물의 평가

이충호,우흥명,권오경,남치주 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.1

The purpose of the present study was to evaluate the easiness of suture and fixation of modified total ring prostheses (m-TRP). Twelve healthy dogs (B.W.2-10 kg) were randomly assigned to three groups. In group A (3 dogs), total ring prostheses was fixed by penetrating a suture material through tracheal mucosa. In group B (3 dogs), TRP was fixed by suturing on tracheal cartilage and muscular layer not penetrating through tracheal mucosa. m-TRP was applied to the cervical portions (group M-C,3 dogs), and thoracic portions (group M-T,3 dogs). Operating time of group M-C (37.33$\pm$6.80 min.) was shorter than those of groups B (83.33$\pm$8.50 min.) and A (63.33$\pm$11.06 min.) (p<0.01). Clinical complications were minimal and limited to mild, short-term hematoma, vomiting, edema, and inflammation. Coughing remarkably decreased in group B rather than group A. Dyspnea was not showed in group A, B, and M-C, but group M-T had a mild dyspnea. Gross postmortem findings were similar in all groups. Mild adhesions were present between prostheses and adjacent structures, but tracheal lumen was clean. Severe adhesions were present where m-TRP had been applied in the thoracic portions. Histopathologic abnormalities included mild to moderate adventitial and periprosthetic fibrosis and mild adventitial inflammation. The present study indicated that m-TRP were easier in suture and fixation than TRP and had no differences in support for trachea and side effects.