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국산 프로폴리스의 Helicobacter pyrori 억제효과
한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1
Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.
한상미 ( Sang-mi Han ),우순옥 ( Soon-ok Woo ),김세건 ( Se-gun Kim ),장혜리 ( Hye-ri Jang ) 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained 63.9±5.4% melittin, 10.9±1.6% phospholipase A2, and 2.3±0.3% apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below 1 μg/mL, but cytotoxicity at 10 μg/mL and above. As a result of the increase in the synthesis of β-casein, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cytotoxicity, the β-casein content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the expression of bovine β-casein (bCSNB) mRNA, a β-casein synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of livestock by ultimately increasing the expression of milk protein
환원제 Sodium Hydrosulfite를 이용한 천연쪽의 견 염색효과
정인모 ( Chung In Mo ),우순옥 ( Woo Soon Ok ) 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.2
천연쪽 색소를 사용하는 의한 견직물의 염색 방법을 확립하고자 시험결과 환원제인 Sodium Hydrosulfilte를 사용한 경우 염색온도가 30℃에서는 30분, 50℃에서는 10분 염색 한 것이 표면 염착농도(K/S)가 가장 높았고, 염색액의 pH 7.4정도인 환원제 및 염료량을 4g/ℓ일 경우에 가장 높았다. 1회 염색직물의 표면염착농도(K/S)는 개선은 7.20, 기존 염색 방법은 1.09이었으며, 염색 직물의 분리한 색소와 염료색소는 모두 청색색소와 적색색소의 2개의 성분이었고 분자량은 262이었으며, 기존과 개선 모두 정균율은 99.8%이상으로 항균성이 우수하였다. Effect of reducing agent sodium hydrosulfite on the natural indigo on the silk fabrics were examined to improve traditional dyeing method. K/S values of dyed fabrics was monitored with various dyeing temperature time, amount of dye and reducing agent. The dyeability of silk fabrics was improved using reducing agent,k sodium hydrosulfite; K/S value(7.20)was higher then that (1.09) of traditional method at the frist dyeing. Natural indigo dye extracted from dyed silk fabrics are composed of isomer, indigo(67.3%)and indirubin(32.5%). However, silk fabrics showed excellent antimicrobial activity regardless of the dyeing methods.