http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
hESC로부터 유래된 혈관내피전구세포를 위한 최적의 배양조건 확립
김주미 ( Ju Mi Kim ),문성환 ( Sung Hwan Moon ),이민지 ( Min Ji Lee ),오인록 ( In Rok Oh ),신정민 ( Jeong Min Shin ),박순정 ( Soon Jung Park ),정선화 ( Sun Hwa Chung ),김문규 ( Mun Kyou Kim ),이경일 ( Kyung Il Lee ),정형민 ( Hyung 한국조직공학과 재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
Human embryonic stem cell(hESC) can be very valuable source for the cell therapy due to their pluripotency when they differentiated into specialized cell types. Conventionally, differentiated cell population were obtained from spontaneously formed embryoid body(EB) which could contain various cell types. In this study, we described the optimization of culture condition for endothelial precursor cells derived from human embryonic stem cells(hESC-EPCs). First, we compared the expression of endothelial precursor cell markers such as CD133, CD34 and KDR in day 7 hEBs and isolated CD133 and KDR double positive population as hESC-EPCs. Second, we compared the culture medium and ECM coating to optimize culture condition for hESC-EPCs. As the result, we could confirm EGM-2 and collagen coating were optimized condition to culture of hESC-EPCs. After conducting optimized culture condition for hESC-EPCs, we examined the maintenance of characteristics of hESC-EPCs cultured in optimized culture condition as vascular cells. Finally, we could confirm the characteristics of hESC-EPCs cultured in optimized condition as vascular cells were maintained in developed optimized culture condition. Furthermore, the optimized culture condition developed in this study will be useful to culture of other vascular lineage cells derived from hESCs.