高速液體 크로마토그래피에 依한 人蔘 및 人蔘製品中의 遊離糖의 定量

崔鎭浩(Jin Ho Choi),張辰奎(Jin Gyu Jang),朴吉童(Kil Dong Park),朴明漢(Myung Han Park),吳成基(Sung Ki Oh) 한국식품과학회 1981 한국식품과학회지 Vol.13 No.2

Studies on the Anti-aging Action of Korean Ginseng

Jin-Ho Choi(崔鎭浩),Sung-Ki Oh(吳成基) 한국식품과학회 1985 한국식품과학회지 Vol.17 No.6

Isolation of Ginsenoside-Rh<SUB>1</SUB> and -Rh<SUB>2</SUB> by High Performance Liquid Chromatography

Jin Ho Choi(崔鎭浩),Woo Jung Kim(金友政),Soon Keun Hong(洪淳根),Sung Ki Oh(吳成基),Hikokichi Oura(大浦 彦吉) 한국식품과학회 1981 한국식품과학회지 Vol.13 No.1

人蔘의 加工方法에 따른 Vitamin B 群의 安定性에 관한 硏究

최진호(Jin Ho Choi),박길동(Kil Dong park),성현순(Hyun Soon Sung),이광승(Kwang Sung Lee),오성기(Sung Ki Oh) 한국식품영양과학회 1982 한국식품영양과학회지 Vol.11 No.2

人蔘의 非사포닌成分인 vitamin 중 生體內에서 補酵素로서 各種代謝 및 生理作用과 關係가 깊은 vitamin B群의 含量變化를 HPLC法으로 定量하여 人蔘加工方法에 따른 vitamin B群의 安定性을 檢討한 結果는 다음과 같다.<br/> 1. 人蔘類중의 vitamin B群의 定量은 逆相 column인 μ Bondapak C_(18)을 사용한 HPLC 方法이 效果的이었다.<br/> 2. UV 檢出器의 適正波長은 移動相의 液性을 pH3.5로 調整하였을 때 niacin, niacin amide, riboflavin 및 thiamine은 254㎚, pyridoxine은 280㎚, cobalamine은 365㎚에서 分析함이 效果的이었다.<br/> 3. 移動相은 PIC B-6을 사용한 water/methanol system을 利用하여 混合比와 流速은 78/22, 1.2mι/min으로 하여 niacin, niacin amide, 및 cobalamine을, 또 74/26, 0.9mι/min으로 하여 riboflavin 및 thiamine을 分析함이 效果的이었다.<br/> 4. 人蔘加工方法에 따른 vitamin B群의 含量變化를 보면 niacin 및 niacin amide는 55.9~61.5r/g 및 13.2~14.5r/g이고 cobalamine은 4.9~5.4mr/g이며 riboflavin 및 thiamine 은 3.9~4.8r/g 및 6.9~8.2 r/g으로서, 含量變化의 폭이 크지 않았다. 따라서 vitamin B群의 安定性은 대체로 높은 傾向을 나타내고 있었다. B-vitamin contents in ginsengs ranged from 55.9 to 65.5 r/g and from 13.2 to 14.5 r/g for niacin and niacin amide, from 4.9 to 5.4 mr/g for cobalamine, and from 3.9 to 8.2 r/g and from 6.9 to 8.2 r/g for riboflavin and thiamine regardless processing. Especially, cobalamine was detected in ginseng.<br/> It is found that the contents of B-vitamins somewhat decreased according to processing-methods, washing-dried and steaming-dried of fresh ginseng. Niacin, niacin amide and cobalamine decreased by 6~9%. Therefore, it is approved that B-vitamins were stable in processing methods.

Ginsenosides의 處理溫度 및 時間에 따른 反應速度論的 硏究

崔鎭浩(Jin Ho Choi),金斗河(Doo Ha Kim),成絢淳(Hyun Soon Sung),金友政(Woo Jung Kim),吳成基(Sung Ki Oh) 한국식품과학회 1982 한국식품과학회지 Vol.14 No.3

고려인삼의 노화억제작용에 관한 연구 ( 2 ) 디올 및 트리올사포닌의 과산화지질 생성억제효과

최진호,오성기 ( Jin Ho Choi,Sung Ki Oh ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4

The inhibitory effects of diol, triol and total saponins isolated from red ginseng on the lipoperoxide formation in vitro and in vivo were studied and correlated with anti-aging. To this end, antioxidant activity, induction period and lipoperoxide were measured by the methods of electron donating ability(EDA), peroxide value(POV) and thiobarbituric acid (TBA) value. Also superoxide dismutase (SOD) and peroxidase activities were measured by the pyrogallol autoxidation method (ΔA 420 ㎚/min) and initial velocity(ΔA 436 ㎚/ min), respectively. Diol and triol saponins were fractionated by Kiesel gel column chromatography (CHCl₃ MeOH-H₂O, 65:35:10, lower phase) and identified using authentic ginsenosides. The compositions of diol and triol saponins were analyzed by high performance liquid chromatography(HPLC). The comparative experiments of EDA, TBA value and induction period in vitro were carried out, and it was proved that triol saponin was more effective than diol saponin. This may be explained by the structural feature that triol saponin in contrast with diol saponin does not have glycoside linkage at the C₃ OH residue of 20(S)protopanaxatriol, and the hydroxyl group of C₃-position in triol saponin seems to be closely related to the antioxidative action. From the comparative study of diol and triol saponins on intraperitoneal (i.p.) and/or oral (p.o.) administrations, it was found that triol saponin was more effective than diol saponin in the inhibitory effects on the lipoperoxide formation of liver, kidney and blood. From the comparative experiments of superoxide dismutase(SOD) activity for both the i.p. and p.o. administrations, it was similarly indicated that the triol saponin group showed remarkable inhibitory effects in comparison with the diol saponin group. This may be due to the scavenger of superoxide radical (O₂^-·) by SOD in liver. In particular, the orally administered group showed more stronger inhibitory effects on the lipid peroxidation in comparison with the intraperitoneally administered group, suggesting that the continuous oral administration was more effective than the temporary administration. The peroxidase activity also showed a similar trend to the SOD activity in vitro and in vivo experiments.

Studies on the Anti - aging Action of Korean Ginseng - (Ⅰ) Comparative Study of Red and White Ginsengs on Anti - aging Action

최진호(Jin Ho Choi),오성기(Sung Ki Oh) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.4

紅蔘과 白蔘의 抗酸化作用에 의한 老化抑制作用을 究明하기 위하여 80% ethanol extract 및 水飽和부탄올로 移行한 saponin을 試料로 하여 in vitro 및 in vivo 實驗을 통하여 紅蔘과 白蔘의 老化抑制作用을 比較하였다.<br/> DPPH에 대한 電子供與能(EDA), TBA value 및 peroxide value에 의한 過酸化脂質生成抑制作用, superoxide dismutase 및 peroxidase의 活性測定등으로 紅蔘과 白蔘의 老化抑制作用을 比較환 結果, 紅蔘은 長期間에 걸친 連續投與에서 白蔘보다 過酸化脂質生成抑制作用. superoxide dismutase 및 peroxidase의 活性을 有意性있게 增加하였다. 生體內 實驗에서 紅蔘이 白蔘보다 老化抑制作用이 현저한 것은 蒸蔘 및 加工處理中 saponin pattern의 變化에 起因하며 白蔘의 PT/PD ratio가 0.401인데 反해 紅蔘의 PT/PD ratio가 0.561로서 triol saponin이 增加되었기 때문이다. The inhibitory effects of the extract and crude saponin of red and white ginsengs on lipoperoxide formation in vitro and in vivo were studied and correlated with anti-aging. To this end, antioxidant activity, induction period and lipoperoxide were measured by the methods of EDA, POV and TBA value. And also superoxide dismutase and peroxidase activity were measured by pyrogallol autoxidation method (ΔA 420/min) and initial velocity(ΔA 436/min), respectively.<br/> From HPLC analysis, the PT/PD ratio of red and white ginsengs was found to be 0.561% and 0.401%, respectively, and red ginseng increased the PT/PD ratio in comparison with white ginseng. The EDA activity of red ginseng was higher than that of white ginseng; red ginseng showed stronger antioxidative effect than white ginseng.<br/> The inhibitory effect of red ginseng was lower than that of white ginseng during the induction period. It was proved that high molecular coloring substance was deeply related to the initial stage of lipoperoxidation.<br/> There was no significant difference between red and white ginsengs in both in vitro and intraperitoneal administration experiments, and red ginseng was more effective than white ginseng in long-term administration. And also inhibitory effect on lipoperoxide formation was mainly occurred in liver, suggesting that the function of liver played an important role in anti-aging actions.<br/> From the measurement of superoxide dismutase(SOD) activity for both ginseng groups intraperitoneally and orally administered, it was found that red ginseng group administered extract and crude saponin showed remarkable inhibitory effects in comparison with white ginseng. In particular, orally administered group showed more stronger inhibitory effect on lipid peroxidation in comparison with intraperitoneally administered group. It was also found that. the continuous oral administration was more effective than temporary administration.<br/> Red ginseng was more notable anti-aging effect in comparison with white ginseng in vivo, and this may be due to the increase of SOD activity in rat-liver. Peroxidase activity also showed similar trend to SOD activity in vitro and in vivo experiments.<br/> Red ginseng was not only superior to white ginseng for preservation but also for biochemical and pharmaceutical efficacy.

A Comparative Study of High - Performance Liquid Chromatographic Method for Analysis of Ginseng Saponin

최진호(Jin Ho Choi),박길동(Kil Dong Park),한강완(Kang Wan Han),오성기(Sung Ki Oh) 한국식품영양과학회 1982 한국식품영양과학회지 Vol.11 No.3

人蔘사포닌중의 各 ginsenosides를 效果的으로 分離 定量하기 위하여 carbohydrate analysis column을 使用한 HPLC로 典型的인 溶媒 system인 acetonitrile/water의 混合比率을 80/20에서 94/6까지 調整하여 retention time과 分離能과의 關係를 比較試驗하였고 또 n-butanol의 添加效果도 調査하였다. 基本構造가 다른 diol saponin과 triol saponin을 같은 mobile phase로 만족하게 分離定量하기는 어렵다.<br/> 따라서 diol saponin은 acetonitrile/water system(80/20), trol saponin은 acetonitrile/water/n-butanol system(86/14/10)을 mobile phase로 하여 分析함이 效果的이었다. 이 方法에 따라 白蔘과 紅蔘을 定量한 結果, diol saponin 含量은 큰 差異가 없으나 triol saponin 含量은 紅蔘이 白蔘보다 增加하였다. 特히 PT/PD ratin가 白蔘은 0.401인데 比해 紅蔘은 0.561로서 紅蔘이 白蔘보다 약 1.4倍나 높았다. 이것이 紅蔘의 生化學的藥理效能과 깊은 關係가 있다고 判斷되며 熱處理에 의한 製造工程과 關係가 있다. Ginseng saponins separated from Panax ginseng C.A. Meyer were analyzed by high-performance liquid chromatography using a carbohydrate analysis column. The effect on the resolution and retention time of each ginsenosides, as well as the addition effect of n-butanol on a acetonitrile/water system, was examined using various proportions of acetonitrile/water system (80/20-94/6) which have been used with typical solvent of carbohydrate analysis column.<br/> The retention time of each ginsenosides was greatly affected by the compositions and mixture proportions of the mobile phase and also markedly increased as the proprotion of acetonitrile in mobile phase increased. It was proved that acetonitrile/water system (80/20) and acetonitrile/water/n-butanol system (86/14/10) were very effective mobile phases for diol and triol sapoin analyses, respectively.<br/> According to the result obtained by this method, the PT/PD ratios of white and red ginseng saponins were 0.401 % and 0.561 %, respectively. Red ginseng increased PT/PD ratio, compared with white ginseng. This is attributed to the change of saponin pattern by processing method and the change of PT/PD ratio would greatly influence on the biochemical and pharmacological effects of ginseng and its products.

Studies on the Anti - aging Action of Korean Ginseng

최진호,오성기,Choi, Jin-Ho,Oh, Sung-Ki Korean Society of Food Science and Technology 1985 한국식품과학회지 Vol.17 No.6

홍삼(紅蔘)사포닌 가수분해물(加水分解物)의 항산화작용(抗酸化作用)에 의한 노화억제작용(老化抑制作用)을 구명(究明)하기 위하여 홍삼(紅蔘)사포닌을 가수분해(加水分解)하여 얻은 prosapogenin, panaxadiol 및 panaxatriol을 시료(試料)로 하여 in vitro 및 in vivo 실험(實驗)을 통하여 이들 가수분해물(加水分解物)들의 생체(生體) 과산화지질생성(過酸化脂質生成)에 미치는 억제효과(抑制效果)를 비교(比較)하였다. DPPH에 대한 전자공여능(電子供與能)(EDA)은 $13.88{\sim}19.76%$를 나타내고 있었으며 in vitro에서 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)는 현저하였으며 그 순서는 prosapogenin>panaxatriol>panacadiol이었다. POV에 의한 유효기간(誘導期間)은 이들 가수분해물(加水分解物) 홍삼(紅蔘)사포닌보다 훨씬 높았으며 prosaposenin>panaxatriol>panaxadiol의 순이었다. in vivo에서 복강(復腔)(i. p.) 및 경구(經口)(p.o.) 투여(投與)에 의한 생체내(生體內) 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)는 대조군(對照群)에 비(比)해 훨씬 효과적(效果的)이었으며, 홍삼(紅蔘)사포닌과 비교(比較)했을 때 간장(肝臟)과 신장(腎臟)에는 거의 비슷한 효과(效果)를 나타냈지만 혈액(血液)에서는 이들 가수분해물(加水分解物)이 홍삼(紅蔘)사포닌보다 훨씬 효과적(效果的)이었다. 효소활성(酵素活性)으로서 superoxide dismutase 활성(活性)을 in vitro에서 비교해 보면 이들 가수분해물(加水分解物)이 $24.2{\sim}36.4%$의 과산화지질생성(過酸化脂質生成) 억제효과(抑制效果)를 나타내고 있어, 홍삼(紅蔘)사포닌의 12.1%보다 $2{\sim}3$ 배(倍)의 효과(效果)를 나타내고 있었고, in vivo에서는 복강(復腔)(i. p.) 투여(投與)에서는 $12.9{\sim}22.2%$. 경구(經口)(p.o.) 투여(投與)에서는 $11.2{\sim}21.6%$의 높은 활성(活性)을 나타내고 있었다. 단 peroxidase 활성(活性)은 복강(復腔)(i. p.) 투여(投與)에서는 $129.0{\sim}188.6%$, 경구(經口)(p. o.) 투여(投與)에서는 $111.4{\sim}139.6%$의 활성(活性)을 나타내고 있었다. The inhibitory effects of red-ginseng saponin hydrolyzates (prosapogenin, panaxadiol and panaxatriol) on lipoperoxide formation in vitro and in vivo were investigated and correlated with anti-aging. Saponin hydrolyzates showed the electron-donating ability (EDA) of 12.88 - 19.76% to DPPH in vitro, and the ability was distinctively decreased in order of prosapogenin, panaxatriol and panaxadiol. The induction period of saponin hydrolyzates, which was measured by the method of peroxide value (POV), was much longer than red-ginseng saponin and decreased in order of prosapogenin, panaxatriol and panaxadiol. The inhibitory effect of saponin, hydrolyzates in vivo was remarkably greater than control. In contrast to red-ginseng saponin, almost similar inhibitory effect in rat liver and kidney was observed, whereas they were much more effective than red-ginseng saponin in blood. The superoxide dismutase (SOD) activity of saponin hydrolyzates in vitro was also measured, and the inhibitory effect of saponin hydrolyzates was found to be 24.2-36.4% and 2-3 times greater than that of red-ginseng saponin (12.1%). Saponin hydrolyzates showed the inhibitory effects of 11.2-21.6% and 12.9-22.2% in oral and intraperitioneal administrations, respectively. It was also found from the measurement of peroxidase activity that the inhibitory effects of saponin hydrolyzates were 111.4-139.6% in oral administration and 129.0-188.6% in intraperitoneal administration.

Larqe guantity isolation of Ginsenoside $-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re\;and\;-Rg_1$ in Panax ginseng C.A. Meyer by High Performance Liquid Chromatography

최진호,김우정,배효원,오성기,대포언길,Choi, Jin-Ho,Kim, Woo-Jung,Bae, Hyo-Won,Oh, Sung-Ki,Oura, Hikokichi 한국응용생명화학회 1980 Applied Biological Chemistry (Appl Biol Chem) Vol.23 No.4

인삼의 유효약리성분으로 밝혀진 saponin중의 각 ginsenosides를 효과적이고 능률적으로 분리하기 위하여 대량분취전용 고속액체 chromatograph인 preparative HPLC의 응용을 검토하였다. 조(粗) saponin획분을 preparative HPLC인 Prep LC/system-500를 사용하여 부분분획을 하고 각 획분에 함유되어 있는 ginsenosides의 조성을 Analytical HPLC로 동정한 후 Semi-preparative HPLC를 사용하여 인삼주성분 saponin을 단리했다. 그 결과 인삼 주성분 saponin인 $ginsenoside-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re$ 및 $-Rg_1$은 약 20 mg / 2.0 ml / injection으로 chromatography를 행하여 $300{\sim}400mg/day$로 대량분취가 가능하였다. 따라서 ginsenosides의 약리 및 임상효능 연구에 크게 기여하게 될 것이다. Relatively large quantity of the major components of saponin, $ginsenoside-Rb_1,\;-Rb_2,\;-Rc,\;-Rd,\;-Re\;and\;-Rg_1$ from Panax ginseng C.A. Meyer were isolated using preparative and semipreparative high performance liquid chromatography, and analyzed by analytical HPLC. The application of HPLC for isolation of ginsenosides was not only very effective for rapid analysis but also reduced the isolation time. The isolation capacity of pure ginsenosides was $30{\sim}50mg/hr$.