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The Fabrication of Cell Chips for Use as Bio-sensors
예철헌,민준홍,최정우 한국바이오칩학회 2007 BioChip Journal Vol.1 No.4
The development of biosensors for the detection of chemicals that are used by cells is of great interest to the pharmaceutical and environmental fields because of the potential for biosensors to measure unknown materials that cannot be detected based on their DNA or protein reactions. However, various problems associated with the introduction of cells to micro-devices, such as low reproducibility and sensitivity, must be overcome before biosensors can be used on a commercial scale. Here, we briefly discuss the cultivation of cells within microchips <i>in vitro</i> as well as the biosensor technologies that allow detection of cell properties and subsequent evaluation of specific analytes.
Waleed Ahmed El-Said,예철헌,권일근,최정우 한국바이오칩학회 2009 BioChip Journal Vol.3 No.2
The effect of anticancer drugs and toxins on the viabiity of HepG2 cells were examined by the cyclic voltammetry (CV) and the potentiometric stripping analysis (PSA) methods. The cells were immobilized on gold patterned silicon substrate. The voltammetric behaviors of HepG2 cells showed a quasi-reversible process and the peak current showed a linear relationship with cell number. The attached living cells were treated with different concentrations of anticancer drugs and toxin. As the exposed concentrations of anticancer drugs and toxins were increased, we observed that the peak current in CV assay and the area under the peak in PSA assay were decreased. Trypan blue dyeing experiment was performed to confirm the results of the effects of anticancer drugs on the cell viability which were obtained from CV assay and PSA assay. These results indicate that the proposed direct cell immobilization method technique can be applied to construct the cell chip for the diagnosis, drug detection, and on-site monitoring depended on the voltammetric and PSA methods.