구강 상주균에 대한 편백 피톤치드의 항균효과

어규식,홍정표,전양현,Auh, Q-Schick,Hong, Jung-Pyo,Chun, Yang-Hyun 대한안면통증구강내과학회 2009 Journal of Oral Medicine and Pain Vol.34 No.4

The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of the surviving resident oral bacteria on F. nucleatum. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). The surviving salivary bacterium were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against F. nucleatum, the isolates from the phytoncide-treated saliva were cultured with F. nucleatum. The results are as follows: 1. Among the 200 surviving resident oral bacterium, 70(35.0%) bacterium inhibit the growth of F. nucleatum on blood agar plates. 2. Among the 70 bacterium which inhibit F. nucleatum, Streptococcus salivarius was 41.3%(45/109), Streptococcus sanguinis was 28%.(7/25), Streptococcus mitis was 20%(3/15), Streptococcus parasanguinis was 33.3%(3/9), Streptococcus Alactolyticus was 100%(8/8), Streptococcus vestibularis was 28.6%(2/7) and Streptococcus sp. was 50%(2/4). Taken together, among the surviving resident oral bacterium, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus mitis were mainly observed to inhibit F. nucleatum. and they may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used to prevent and cease the progress of periodontal disease, halitosis. Thus it is expected to promote oral health.

어떻게 평가할 것인가?

어규식(Q-Schick Auh) 대한치과의사협회 2010 대한치과의사협회지 Vol.48 No.3

Snoring is a very common sleep disorder. Approximate 20-30 percent of population and 40-60 percent of middle and old age population are reported to have symptoms of snoring. Snoring patients do not frequently recognize snoring until spouse or colleague has noticed. Hence, for most people snoring has been not a serious health issue but one of bad sleeping habits. However, it recently draws social attention because most patients with diagnosed “Obstructive Sleep Apnea Syndrome” cased by obstruction of the upper airway are reported snoring. In this point, the author attempts to review the clinical aspects and treatments about snoring and “Obstructive Sleep Apnea Syndrome” in the medical and dental perspective.

전신질환으로 입원한 환자의 구강질환과 전신질환의 상관관계(I)

전양현,어규식,홍정표,Chun, Yang-Hyun,Auh, Q-Schick,Hong, Jung-Pyo 대한안면통증구강내과학회 2008 Journal of Oral Medicine and Pain Vol.33 No.2

Purpose : To investigate the actual conditions of diagnosis and treatment of oral disease of inpatient with systemic disease. Methods : A total of 110 subjects, inpatient due to systemic disease for diagnosis and treatment of oral disease was requested to answer the medical history and dental treatment record. Results : In the main systemic disease, Endocrine, nutritional and metabolic diseases is composed of Gingivitis and periodontal diseases 44.9%, Diseases of salivary glands 22.4%, Within Normal Limit, Dental caries 12.2%, Diseases of pulp and periapical tissues 4.1%, Embedded and impacted teeth, Other diseases of hard tissues of teeth 2%. In the main oral disease, Gingivitis and periodontal diseases is composed of Non-insulin-dependent diabetes mellitus 39.2%, Cerebral infarction 29.4%, Nerve root and plexus disorders 5.6%, Intracerebral hemorrhage 3.9%, Malignant neoplasm of stomach, Thyrotoxicosis, Schizophrenia, Alcoholic liver disease, Nephrotic syndrome 2%. Conclusion : These findings indicate that inpatient due to the systemic disease is significantly correlated to the oral disease. The patients of oral disease interrelationship between inpatient and outpatient of systemic disease should be validated by future research.

Secondary Dental Pain and Facial Pain Due to Pansinusitis : A Case Report

김남구,어규식,전양현,홍정표,Kim, Nam-Koo,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo Korean Academy of Orofacial Pain and Oral Medicine 2007 Journal of Oral Medicine and Pain Vol.32 No.3

Toothache is a common complaint in the dental office. Most toothaches have their origin in the pulpal tissues or periodontal structures1). These odontogenic pains are managed well and predictably by dental therapies. One of the most frequent encounters and most confusing phenomena with which the dental diagnostician must deal is the problem of referred pain. The most important step toward proper management of a toothache is to consider that the pain may not be of dental origin. And Patients with orofacial pain, especially those in chronic pain, present a diagnostic and management challenge for the practitioners. There are many structures in the head and neck that can produce heterotopic pains felt in the teeth and other structures. Once referred pain is suspected, the true source of the pain must be located in order to render effective therapy. With increased interest in temporomandibular disorders and orofacial pain, many studies of accurate diagnosis and differential diagnosis about orofacial pain have been established. The purpose of this paper is to present a case for pansinusitis which produced pain referral in teeth and mimicked the symptoms of migraine.

긴장성 두통 환자에서의 측두하악부 관절-근육통의 양상

오병섭,어규식,홍정표,전양현,Oh, Byung-Sub,Auh, Q-Schick,Hong, Jung-Pyo,Chun, Yang-Hyun 대한안면통증구강내과학회 2007 Journal of Oral Medicine and Pain Vol.32 No.1

Although most cases of tension-type headache(TTH) are myogenic headache, in some cases, arthralgia appears, such as the disorder of the temporamandibular joint. This study is designed to compare the clinical patterns of arthralgia to those of myalgia, when both symptoms are co-existing. Among 167 patients who visit our clinic during a certain period, whose chief complaint was TTH, 18 patients were the arthralgia group, 50 patients were the myalgia group, and 99 patients who have both arthralgia and myalgia were the arthromyalgia group. Three groups were asked to answer the questionnaires about their age, gender, and oral parafunction such as bruxism or clenching. Then we gathered statistics on the data from the accomplished questionnaires. 1. There was statistical significance in age among the three groups(p=0.02). 2. There was no statistical significance in sex and oral parafunction among the three groups. 3. There was statistical significance the myalgia group was older than the arthromyalgia group(p<0.03). The results of the study show that as patients become older, TTH with myalgia happens more frequently than TTH with arthralgia and myalgia does.

경피신경자극치료기(TENS)의 기능별 주파수에 따른 교근과 피개상피의 혈류변화에 관한 연구

조성국,어규식,전양현,홍정표,Cho, Sung-Guk,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo 대한안면통증구강내과학회 2009 Journal of Oral Medicine and Pain Vol.34 No.1

The following results were obtained, after experimenting on change of masseter muscle and bloodstream epithelium with Transcutaneous Electrical Nerve Stimulator(TENS), among 16 male adults. 1. According to applying TENS, it was observed that bloodstream in muscle increases at 1.5, 3.0 Hz. 2. According to applying TENS, it was observed that concentration of moving blood cells in muscle increase at 1.5, 3.0, 6.0 Hz. 3. According to applying TENS, it was observed that velocity of bloodstream in muscle increases only at 1.5 Hz 4. Through experiment, applying TENS at level of 6.0 and 10.0 Hz, all bloodstream, concentration of moving blood cells, and velocity of bloodstream increasing rates were lower in muscle compared to of them in overlying epithelium; and especially increasing bloodstream and its velocity were most frequent at 6.0 Hz, and bloodstream of 10.0 Hz. From the results above when a physical therapy of TENS is carried out the frequency of 1.5, 3.0 H is effective, and as the frequency increases it is disadvantageous to the muscle.

피톤치드의 입냄새 제거효과

박재봉,어규식,전양현,이진용,홍정표,Park, Jae-Bong,Auh, Q-Schick,Chun, Yang-Hyun,Lee, Jin-Yong,Hong, Jung-Pyo 대한안면통증구강내과학회 2007 Journal of Oral Medicine and Pain Vol.32 No.2

Antimicrobial action of phytoncide in the mouth decrease odor-producing microorganisms. Also phytoncide has malodor effect by reaction with volatile sulfur compounds. Phytoncide has excellent malodor effect in microbiologically and chemically. This study prove the malodor effect of phytoncide by use ferrous sulfate. So I try to make new treatment method for halitosis. I get the results as follows. 1. The difference of mean value of absorbancy was 0.849 between the mean absorbancy of deposition by add phytoncide to saliva and the saliva only. 2. The difference of mean value of absorbancy was 0.701 between the mean absorbancy of deposition by add phytoncide to distilled water and the distilled water only. 3. The difference of mean value(0.849) in saliva by existence of phytoncide was larger than in double distilled water(0.701) by existence of phytoncide. Therefore, phytoncide make more deposition in saliva than double distilled water by reaction with sulfur compounds. As the results, phytoncide reaction with sulfur compounds in saliva. It take malodor action in liquid state effectively. It is thought, only the toothpaste it knows from in the limit which does not have a side effect by the human body it adds in the oral cavity of the mouth rinse and with the fact that it will be able to use positively in clinic.

저출력 레이저가 구강미생물에 미치는 영향

윤인종,어규식,전양현,홍정표,Yoon, In-Jong,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo 대한안면통증구강내과학회 2010 Journal of Oral Medicine and Pain Vol.35 No.1

This study is to observe the bacteriocidal effect of the Low Level Laser (LLL) against oral microorganisms which are related to the occurrence of periodontal diseases and oral malodors. The Porphyromonas gingivalis 2561 (P. gingivalis 2561) and Prevotella intermedia (Pr. intermedia) were treated with photosensitizing substance-toluidine blue O (TBO; C.I. 52040) and then radiated with the LLL which has 650nm wavelength for 1, 2, 3 and 5mins. continuously upon varying distances of 1, 2 and 3cm for each experimental groups. The results are as follows; 1. The P. gingivalis 2561 which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.99% higher antibacterial effect in comparison to the experimental group treated only with TBO. 2. The Pr. intermedia which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.8% higher antibacterial effect in comparison to the experimental group treated only with TBO. 3. The bacteriocidal effect of the P. gingivalis 2561 treated with TBO was found to gradually increase as the radiation time of LLL extended from 1min. to 3min. at 1min. intervals. 4. A slight decrease in bacteriocidal effect of the P. gingivalis 2561 was found as the radiation distance of LLL increased from 1cm to 3cm at 1cm intervals. 5. The bacteriocidal effect of the Pr. intermedia was found to slightly decrease as the radiation distance of LLL increased from 1cm to 3cm at 1cm. intervals. As the results shown above suggest, the bacteriocidal effect of LLL was found to increase as the radiation time extended and the distance shortened. Moreover, even the experimental group radiated with LLL at 3cm distance for 1min. which showed the lowest level of bacteriocidal effect, was found to have 99.8% higher bacteriocidal effect than the experimental group which was treated only with TBO and, therefore, this clearly shows the bacteriocidal effect of LLL against oral microorganisms. Thus, the use of LLL is thought to become very useful for suportive treatment for periodontitis and implantitis, and controlling oral malodors as long as it is used within the limits where there is no side effect.

피톤치드 처리 후의 잔존 구강 세균이 Pr. intermedia에 미치는 영향

박재봉,어규식,전양현,홍정표,Park, Jae-Bong,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo 대한안면통증구강내과학회 2009 Journal of Oral Medicine and Pain Vol.34 No.2

The present study was performed to observe the effect of phytoncide on oral normal microflora and the inhibitory effect of the surviving resident oral bacteria on Pr. intermedia. In this study, saliva from each of 20 healthy subjects was treated with 1% phytoncide from Japanese Hinoki (Chamaecyparis obtusa Sieb. et Zucc.). Surviving salivary bacteria were isolated on blood agar plates and identified by 16S rDNA sequencing. In order to select inhibitory isolates against Pr. intermedia, the isolates from the phytoncide-treated saliva were cultured with Pr. intermedia. The results were as follows: 1. Among the 200 surviving resident oral bacterium, 148(74.0%) bacterium inhibit the growth of Pr. intermedia on blood agar plates. 2. The 200 surviving resident oral bacterium were 109 Streptococcus salivarius(54.5%), 25 Streptococcus sanguinis(12.5%), 15 Streptococcus mitis(7.5%). 3. Among the 148 bacteria which inhibit Pr. intermedia, Streptococcus salivarius was 85.3%(93/109), Streptococcus sanguinis was 64.0%.(16/25), Streptococcus mitis was 54.3%(8/15), Streptococcus parasanguinis was 66.7%(6/9), and Streptococcus Alactolyticus was 100%(8/8). Taken together, among the surviving resident oral bacterium, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus mitis were mainly observed to inhibit Pr. intermedia. and they may exert an additional inhibitory activity against the periodontopathic bacterium. Therefore, phytoncide can be used for preventing and ceasing the progress of periodontal disease and halitosis, and thus is expect to promote oral health.

편백 피톤치드가 Candida albicans에 미치는 영향에 대한 연구

강수경,어규식,전양현,홍정표,Kang, Soo-Kyung,Auh, Q-Schick,Chun, Yang-Hyun,Hong, Jung-Pyo 대한안면통증구강내과학회 2010 Journal of Oral Medicine and Pain Vol.35 No.1

Phytoncide, essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Candida albicans, which is a commensal colonizer of the mucous membranes but has become an opportunistic pathogen. C. albicans was incubated with or without phytoncide extracted from Hinoki (Chamaecyparis obtusa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its optical density, cell viability and morphology. As concentrations of phytoncide added to the culture medium increased, optical density and cell viability of C. albicans decreased. Minimum inhibitory concentration of phytoncide for C. albicans was observed to be 0.25%, and minimum fungicidal concentration was 0.5%. Numbers of morphologically atypical cells with electron-dense cytoplasm and granules and increased with increasing concentration of the phytoncide. At higher concentrations of phytoncide, compartments and organelles in the cytoplasm became indistinguishable. The overall results indicate that the phytoncide used for this study has a strong antimicrobial activity against C. albicans. Therefore, the phytoncide may be used as a candidate for prevention and therapeutic agent against oral candidiasis.