제3발표장 : 농산물,식품가공공학 분야 ; 과일의 저장기간이 커패시턴스에 미치는 영향

양승환 ( Seung Hwan Yang ),이상대 ( Sang Dae Lee ),김기복 ( Ki Bok Kim ),조병관 ( Byoung Kwan Cho ) 한국농업기계학회 2013 한국농업기계학회 학술발표논문집 Vol.18 No.2

발효 청미래덩굴잎 추출물의 혼합에 의한 토복령의 항산화활성 증진효과

이상일 ( Sang Il Lee ),이예경 ( Ye Kyung Lee ),김순동 ( Soon Dong Kim ),심순미 ( Soon Mi Shim ),양승환 ( Seung Hwan Yang ),성금화 ( Jin Hua Cheng ),서주원 ( Joo Won Suh ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.2

To evaluate the improving effects of antioxidant activity, we observed antioxidant capacities such as electron donating ability (EDA), Ferric reducing antioxidant power (FRAP), inhibitory activity of xanthine oxidase (XO) and aldehyde oxidase (AO), and sensory characteristics on mixture of Smilax china L. root water extract added with water extract of fermented S. china L. leaf by Aspergillus oryzae (FSCL). Those contents of mixture with higher ratio of FSCL were proportionally high. And OD475 of mixture with higher ratio of FSCL was almost proportionally high (R2= 0.9850). Antioxidant capacities of EDA and FRAP of the mixture was higher than that of non-mixture. In addition, XO inhibitory activity (IC50) of A (1.19) was 59.80% higher than that of F (2.96), and the activity of mixture by the higher ratio of FSCL was proportionally low (R2 =0.9490). Taste acceptability of A was slightly higher than that of F, whereas that of C was highest. And color acceptability of 40.80% mixture was higher than those of A, F, and B. Overall acceptability of C and D was highest than those of others. Moreover, hot water extract of S. china L. leaf fermented with A. oryzae was maroon color, which looks like Puerh tea style, and mixture of S. china L. root extract added with hot water extract of S. china L. leaf was high acceptability of beverage. These results suggest that mixture of extract of S. china L. root and hot water extract of S. china L. leaf fermented with A. oryzae could improve antioxidant activities.

농산물,식품가공공학 분야 : P2-4 ; MRI를 이용한 소고기 근내지방 함량 측정

이상대 ( Sang Dae Lee ),김용주 ( Young Joo Kim ),양승환 ( Seung Hwan Yang ),조병관 ( Byoung Kwan Cho ) 한국농업기계학회 2014 한국농업기계학회 학술발표논문집 Vol.19 No.2

Monascus pilosus로 발효시킨 후발효 녹차가 고지방 식이 마우스의 체중과 혈청 지방함량 및 간 조직 항산화계 효소활성에 미치는 영향

이상일 ( Sang Il Lee ),이예경 ( Ye Kyung Lee ),김순동 ( Soon Dong Kim ),양승환 ( Seung Hwan Yang ),서주원 ( Joo Won Suh ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.2

The anti-obese, hypolipidemic and hepatoprotective effects of post-fermented green tea by Monascus pilosus was tested with mice fed with high-fat diet for 7 weeks. The body weight gain and feed efficiency ratio (FER) in normal control group (NC), CHA (2% non-fermented green tea powder supplemented high-fat diet group) and mCHA (2% green tea powder post-fermented by M. pilosus supplemented high fat diet group) groups were significantly lower than those of high fat diet control group (HC). Epididymal fat weight in mCHA and NC were significantly lower than HC. The hepatic lipid peroxide was dramatically higher in HC than that of NC and was significantly lower in CHA and mCHA. In addition, dehydrogenase type activity of xanthine oxidoreductase in HC was lower than that of NC, but significantly higher than CHA and mCHA. In histopathological findings, hepatic fat accumulation in HC was higher than that of NC, CHA and mCHA. Antiobese, hypolipidemic and antifatty liver effect of green tea powder postfermented by M. pilosus was slightly higher than that of nonfermented green tea. In conclusion, the constituents of green tea fermented by M. pilosus has been proven to not only inhibit obesity and hyperlipidemia but also decrease the hepatic fat accumulation in high fat diet-induced obese mice.

천연물 / 유기화학 : 고지방식이로 유도한 비만 흰쥐에서 Monascus pilosus 균사체 추출물의 간 손상 예방효과

이상일 ( Sang Il Lee ),김재원 ( Jae Won Kim ),이예경 ( Ye Kyung Lee ),양승환 ( Seung Hwan Yang ),이인애 ( In Ae Lee ),서주원 ( Joo Won Suh ),김순동 ( Soon Dong Kim ) 한국응용생명화학회(구 한국농화학회) 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.3

Hepatoprotective effects of Monascus pilosus mycelial ethanol extract (MPME) were examined in high-fat diet induced-obese rats. The rats were randomly divided into 2 groups; normal control (NC) and a high-fat and high cholesterol diet group (HFC). The HFC diet group was fed a 5L79 diet supplemented with 15% lard and 1% cholesterol for 3 weeks for induction of obesity. And then, the rats were divided into 4 groups (n=5); the NC, a HFC diet obesity control group (HF), 0.5% MPME supplemented HFC diet group (MPM), and 2% conjugated linoleic acid (CLA) supplemented HFC diet group for 7 weeks. Whereas the daily weight gain of NC and HFC groups were 3.48 g and 4.48 g, respectively, those of MPM and CLA were 3.09 g and 4.38 g, respectively. Furthermore, activity of serum alanine and aspartic aminotransferase in HF was markedly higher than those of NC group, but, the activity in MPM and CLA was significantly lower than HF. Hepatic reduced glutathione content in MPM and CLA was higher than HF. On the contrary, hepatic lipid peroxide content in MPM and CLA was significantly lower than HF. In conclusion, although the precise mechanisms of the hepatoprotective effects of the MPME in this study are unknown, our study provides experimental evidence that MPME may prevent obesity and hepatic damage by high-fat and high cholesterol diet via inhibition of lipid absorption and induction of reactive oxygen spices scavenging enzyme such as superoxide dismutase.

Monascus pilosus로 발효시킨 뽕잎차의 품질특성과 항산화능

이상일(Sang-Il Lee),이예경(Ye-Kyung Lee),최종근(Jongkeun Choi),양승환(Seung Hwan Yang),이인애(In-Ae Lee),서주원(Joo-Won Suh),김순동(Soon-Dong Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.5

Monascus pilosus에 의한 발효뽕잎차(FMM: fermented mulberry leaves tea)의 monacolin K 함량, citrinin의 유무, 관능적 품질, total polyphenol 및 total flavonoid의 함량, ROS 생성계 효소인 xanthine oxidase 저해활성 및 ROS 소거계 효소류의 활성을 건조뽕잎(UFM: unfermented mulberry leaves)과 비교하였다. FMM은 UFM에 비하여 색상기호도 및 종합적 기호도가 유의적으로 높았다. FMM에 함유된 total monacolin K 함량은 0.056%(dry basis)로 활성형이 83.93%를 차지하였으며 citrinin은 검출되지 않았다. Total polyphenol과 total flavonoid의 함량 모두 UFM에서 현저히 높았다. XO 저해활성과 SOD 유사활성은 농도에 비례하여 높았으며 UFM이 FMM에 비하여 높았다. 전자공여능과 ferric-reducing antioxidant power(FRAP)의 경우도 UFM과 FMM 모두에서 농도에 비례하여 증가하였으나, UFM에서 FMM에 비하여 현저히 증가하였다. Ferrous iron chelating 활성의 경우에도 추출물의 첨가 농도에 비례하여 증가하였고 그 증가의 정도는 UFM이 FMM에 비해 더욱 강하게 나타났으나, 4.0 ㎎/mL 이상의 농도에서는 감소하였다. 한편 polyphenol 또는 flavonoid 함량 당의 XO 저해활성과 SOD 유사활성 및 FRAP는 FMM이 UFM에 비하여 현저하게 높았다. 이상의 결과들을 종합해 볼 때, 뽕잎을 M.pilosus로 발효시킨 FMM은 홍국의 기능성을 가짐과 더불어 상당히 강한 항산화능과 기호성을 관찰할 수 있어 ROS로 야기될 수 있는 여러 질병의 예방과 치료에 도움을 줄 수 있는 기초자료가 될 것이라 사료된다. This study was carried out to investigate the contents of monacolin K and citrinin, along with the sensory quality and antioxidant activity of mulberry leaf tea fermented by Monascus pilsous (FMM). Total monacolin K content of FMM was 0.058%, but citrinin was not detected. Redness of brewed FMM was remarkably higher than that of unfermented mulberry leaf tea (UFM). In sensory evaluation of brewed FMM, while astringent taste and savory taste were lower, flavor, color, and overall acceptability were significantly higher than those of UFM. Total polyphenol contents of UFM and FMM were 83.1 and 23.61 ㎎/g (dry basis), total flavonoid contents of UFM and FMM were 17.96 and 3.99 ㎎/g (dry basis), respectively. Xanthine oxidase (XO) inhibitory activity and superoxide dismutase (SOD)-like activity of FMM were lower than those of UFM. Electron-donating ability and ferric-reducing antioxidant power of FMM were slightly lower than those of UFM. However, the antioxidant activities of FMM per polyphenol content were markedly higher than those of UFM. These results suggest that FMM may scavenge excessive reactive oxygen spices (ROS) via inhibition of XO and SOD-like activity. Furthermore, FMM demonstrated relatively higher acceptability and antioxidant ability along with functionality of Hongguk (red yeast rice), and therefore could be utilized to prevent various ROS-induced diseases.

발효 청미래덩굴잎 용매 추출물 및 분획물의 xanthine 및 aldehyde oxidase 저해활성과 항고요산혈증 효과

이상일 ( Sang Il Lee ),이예경 ( Ye Kyung Lee ),김순동 ( Soon Dong Kim ),성금화 ( Jinhua Cheng ),양승환 ( Seung Hwan Yang ),서주원 ( Joo Won Suh ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.1

Aspergillus oryzae로 발효시킨 청미래덩굴잎 용매추출분획물의 xanthine oxidase (XO) 및 aldehyde oxidase (AO) 저해활성과 항고요산혈증에 미치는 영향을 조사하였다. 용매별 추출수율(g/kg)은 80% ethanol (EtOH)은 13.56이었으며, n-hexane, dichloromethane (DICM), ethylacetate (EtOAc) 및 n-butanol fraction (BuOH) 은 1.35.3.33 범위였다. Total polyphenol 함량(mg/g-extract)은 EtOAc fraction 478.07.501.26, BuOH fraction 259.49.289.02, DICM fraction 165.03.232.27, EtOH fraction 134.02.196.54이었으며 EtOAc 및 DICM fraction에서는 발효시킨 경우가 비 발효에 비하여 각각 4.85 및 40.74%가 높았으나 그 외 모든fraction에서는 발효시킨 경우가 낮았다. 총 flavonoid 함량은 EtOAc fraction이 여타 fraction에 비하여 높았다. EtOAc 및 BuOH fraction의 TF 함량은 발효시킨 경우가 비 발효에 비하여 각각 10.56% 및 60.17%가 높았으나 여타 fraction들에서는 발효시킨 경우가 낮았다. XO 저해활성은 모든 분획물에서 발효시킨 경우가 비발효에 비하여 현저하게 높았으며 EtOAc 및 BuOH fraction의 경우 발효시킨 경우는 각각 75.02 및 65.59%로 비발효 경우 39.42 및 5.34% 보다 현저하게 높았다. AO 저해활성은 DICM과 EtOAc fraction에서 각각 81.82 및 77.93%로 여타 fraction들에 비하여 높았으며XO의 경우와 마찬가지로 발효시킨 경우가 비발효에 비하여 현저하게 높았다. 고요산혈증 mouse의 혈중 요산(SU) 함량에 미치는 영향을 조사한 결과, 고요산혈증 대조군의 SU 함량은 6.98mg/dL로 정상대조군 (NC)의 3.82 mg/dL에 비하여 1.83배가 높았으나, EtOAc fraction은 투여량에 의존적으로 SU의 함량이 감소하였으며, 발효시킨 경우가 비발효에 비하여 유의적인 감소를 보였다. 이상의 결과, A. oryzae와 청미래덩굴잎의 발효과정을 통해, glycoside형태의 flavonoid를 aglycone화하여 비발효에 비해 높은 XO 및 AO 저해활성과 함께 항고요산혈증 효과를 나타내는 것으로 사료된다. To evaluate the inhibitory effect of xanthine oxidase (XO) and aldehyde oxidase (AO), and antihyperuricemic effect by Aspergillus oryzae fermented Smilax china L. leaf extracts and fractions, we observed extracted yield by each solvent, the content of total polyphenol and total flavonoid (TF), the activities of XO and AO, and serum uric acid level. Extracted yield (g/kg) by 80% ethanol (EtOH) was 13.56, those of n-hexane, dichloromethane (DICM), ethylacetate (EtOAc) and n-butanol fraction (BuOH) were 1.35.3.33. Furthermore, total polyphenol content (mg/gextract) of EtOAc fraction, BuOH fraction, DICM fraction and EtOH fraction is 478.07.501.26, 259.49.289.02, 165.03.232.27, 134.02.196.54, respectively. Those of fermented EtOAc and DICM fraction was 4.85 and 40.74% higher than that of nonfermented fraction, respectively, while the other fermented fractions were lower than those of non-fermented fractions. And total flavonoid content (mg/g-extract) of EtOAc fraction was higher than those of other fractions. Additionally, TF of fermented EtOAc and BuOH fraction is 10.56 and 60.17% higher, than that of fermented fraction, respectively, although those of the other fermented fractions was lower than that of non-fermented fractions. On the other hand, XO inhibitory activities of all fermented fractions was significantly higher than that of all non-fermented fraction, while those of fermented EtOAc (75.02%) and BuOH fraction (65.59%) was markedly higher than that of non-fermented fraction (39.42 and 5.34%), respectively. In addition, AO inhibitory activities of DICM and EtOAc fraction was 81.82 and 77.93% higher, respectively, than those of the other fractions, and those of fermented fractions as with XO were significantly higher than that of non-fermented fractions. Meanwhile, serum uric acid level (SU) of hyperuricemic control mice (HC, 6.98 mg/dL) was 1.83 folds higher than that of normal control (NC, 3.82 mg/dL). Furthermore, SU in the group treated with EtOAc fraction decreased in a dose dependent manner compared with the allopurinol control group, although those of fermented fractions were significantly lower than those of non-fermented fractions. This study suggests that fermented Smilax china L. leaf extracts may regulate the XO and AO inhibitory activities and antihyperuricemic effect due to aglycone components from glycoside form flavonoids by fermentation of A. oryzae.

2.4㎓ 대역 LTCC 대역통과 여파기의 등가회로 설계

성규제(Gyu-Je Sung),양승환(Seung-Hwan Yang),김동연(Dong-Yon Kim),유재하(Jae-Ha Yoo),여동훈(Dong-Hoon Yeo) 대한전자공학회 2003 대한전자공학회 학술대회 Vol.2003 No.11

반응표면분석에 의한 발효 청미래덩굴(Smilax china L.) 잎 열수 추출조건의 최적화

김재원 ( Jae Won Kim ),이상일 ( Sang Ll Lee ),이예경 ( Ye Kyung Lee ),양승환 ( Seung Hwan Yang ),김순동 ( Soon Dong Kim ),서주원 ( Joo Won Suh ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.5

In this study, we investigated the contents of total polyphenol (TP), total flavonoid, and absorbance at 475 nm (OD475) which may produced in solid-fermented leaf of Smilax china L. by Aspergillus oryzae as a new functional components with reddish brown color, contents of water soluble substance (WSS), electron donating ability (EDA), Hunter L*, a*, b* values, sensory overall acceptability (OA) and also, the inhibitory activities (XOI and AOI) against partial purified xanthine oxidase (XO) and aldehyde oxidase (AO) from rabbit liver which were well known to relate the gout, and alcoholic liver disease, respectively in order to optimize water extraction using response surface methodology (RSM). All the R2 values of the second-order polymonials ranged from 0.85 to 0.98, except for the EDA (0.69) and the XOI (0.78). However, the activities of the EDA and XOI were relatively high in the lower concentration of the fermented Smilax china L. leaf. The effects on the water extraction were highest in the concentration, among the dependent variables, and showed significant differences at the 1% level in the TP, TF and WSS contents and the a*, b* and OD475 values, but the OA showed significant differences at the 5% level. The optimal values of AOI, which was the most important functionality in the Smilax china L. that was predicted via RSM, were 59.48% at the 2.19% concentration, a 90.02°C extraction temperature and a 4.03 minute extraction time (R2: 0.93, p<0.007). The ranges of all the dependent variables of the optimal water extraction were 1.6~1.8% for the concentration, 83~93°C for the temperature and 3.4~4.4 minutes for the extraction time; and the optimal water extraction conditions were a 1.7% concentration, an 88°C extraction temperature and a 3.9-min extraction time.

기초 : 톡소포자충 감염 신경교종세포의 Mitogen-Activated Protein Kinase Pathway 활성화

박병현 ( Byung Hyun Park ),이현성 ( Hyun Sung Lee ),이종수 ( Jong Soo Lee ),양승환 ( Seung Hwan Yang ),신대환 ( Dae Whan Shin ),이영하 ( Young Ha Lee ) 대한뇌종양학회 2006 대한뇌종양학회지 Vol.5 No.2

Objective:Gliomas are the most common primary brain tumors in adults. Toxoplasma gondii is an obligate intracellular parasite with a high affinity for brain cells. Mitogen-activated protein kinases(MAPKs) are known to regulate the host cell invasion, although little is known regarding MAPK signaling in Toxoplasma-infected glioma cells. Methods:U87MG and U373MG cells were infected with tachyzoites of the RH strain of T. gondii, and the kinetics of MAPK activation were determined by immunoblotting. We also determined the effects of MAPK inhibitors on glioma cell growth and T. gondii replication using the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetraterazolium bromide(MTT; Sigma) method and [3H]-uracil incorporation assays. Results:There were no significant differences in the levels of unphosphorylated MAPK at different incubation times in the Toxoplasma-infected U373MG cells. T. gondii induced the activation of extracellular signal-regulated kinase(ERK) 1/2, p30MAPK, and c-Jun N-terminal kinase(JNK)1/2 within 30 minutes of infection, and showed differential kinetics of activation for each MAPK. The activation of ERK1/2, p38MAPK, and JNK1/2 in Toxoplasma-infected glioma cells was blocked by PD98059, SB202190, and SB203580, respectively. T. gondii replication was inhibited in a dose-dependent manner by the addition of MAPK inhibitors to Toxoplasma-infected glioma cells. Conclusion:Infection with T. gondii induces the activation of MAPKs in glioma cells, and this activation can be blocked by the addition of kinase-specific inhibitors. These results suggest that the MAPK pathway plays a role in the invasion of glioma cells with T. gondii.