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- 저자 : 양승오(Seung O Yang) (검색결과 10 건)
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($TGF-{\beta}$)이 Minocycline을 전처리한 사람 치주인대세포의 활성에 미치는 영향
양승오,유형근,신형식,Yang, Seung-Oh,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.2
The initial events required for periodontal regeneration is the attachment, spreading, and proliferation of appropriated cells at the healing sites. These have been reported that minocycline stimulates the attachment of periodontal ligament cells, and also $TGF-{\beta}1$ enhances the proliferation of periodontal ligament cells. The purpose of the present study was to evaluate the effects of $TGF-{\beta}1$ on the cellular activity of minocycline treated human periodontal ligament cells. Periodontal ligament cells were obtained from the explants of healthy periodontal ligaments of extracted 3rd molars or premolar teeth extracted from the patients for orthodontic treatment. The cells were cultured in minimal essential medium(${\alpha}-MEM$) supplemented with 10.000units/ml penicillin, $10,000{\mu}g/ml$ streptomycin and 10% FBS(fetal bovine serum) at $37^{\circ}C$ in a humidified atmosphere of 5% carbon dioxide and the 5th to the 8th passages of the cells were used. To evaluate the effect of minocycline on cell attachment, the cells were seeded at a cell density of $1.5{\times}10^4$ cells/well in 24-well culture plates and treated with $20{\mu}g/ml$ and $100{\mu}g/ml$ of minocycline for 1.5 h. After trypsinization, the cells were counted with hemocytometer and were taken photographs for observation of cellular morphology. To evaluate the effect of $TGF-{\beta}1$ on minocycline-pretreated periodontal ligament cells, the cells were seeded at a cell density of $1{\times}10^4$ cells/ well in 24-well culture plates and treated with $20{\mu}g/ml$ and $100{\mu}g/ml$ of minocycline for 1.5 h. After incubation, 1 and 10ng/ml of $rh-TGF-{\beta}1$ were also added to the each well and incubated for 1 and 2 days, respectively. Then, MTT assay, DNA synthesis($^3H-thymidine\;assay$), and protein and collagen assay(3H-proline assay) were carried out. In the MIT assay, after 200ul MTT solutionlconeentration of 5mg/ml) were added to the each well of the 24-well plates and incubated for 3 hours, and 200 ul DMSO were added so as to dissolve insoluble blue formazan crystals which was formed in incubated period. Then it read plates on a ELISA reader. For mitogenic assay, 1 uCi/ml $^3H-thymidine$ was added to each well for the final 2 hours of the incubation periods. After labeling, the wells were washed 3 times with ice cold PBS and 4 times with 5% TCA to remove unincorporated label and precipitate the cellular DNA. DNA, with the incorporated $^3H-thymidine$, was solubilized with 500 ul of 0.1% NaOH/0.1% SDS. A 250 ul aliquot was removed from each well and placed in a scintillation vial with 4ml of scintillation cocktail. Using an liguid scintillation counter, counts per minute(CPM) were determined for each samples. 3 uCi/ml $^3H-proline$ was added to each well for the final 4 hours of the incubation periods and total protein and percent collagen synthesis were carried out. The results indicate that minocycline treated group with $100{\mu}g/ml$ concentration for 1.5 hours significantly increased than that of control in cell attachment, and cell process is also evident compared with that of control in cell morphology, and the cellular activity and DNA synthesis rate of cells treated minocycline and $TGF-{\beta}1$ significantly increased than that of control values, but were below to values of the $TGF-{\beta}1$ only treated group in MIT assay and $^3H-thymidine\;assay$, and the total protein synthesis of minocycline and $TGF-{\beta}1$ treated group also significantly increased than that of control values, but the percent collagen synthesis of tested group significantly decreased to compared with control. On the above the findings, the tested group of minocycline and $TGF-{\beta}1$ did not increase the eff
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Chlorhxidine 구강 양치용액이 치은연하 세균에 미치는 영향에 대한 연구
임홍기,양승오,신형식,Lim, Hong-Ki,Yang, Seung-Oh,Shin, Hyung-Shik 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.3
The purpose of this study was to assess the clinical and microbiological effect of chlorhexidine rinse following scaling and root planing on periodontits. 10 patients with periodontal disease were selected for the study. They had not taken antibiotics for months and no history of dental treatment for 6 months before the study. They were good in general health. Patients received a scaling and root planing under local infiltration anesthesia, chlorhexidine rise group were subjected to twice a day 0.1% chlorhexidine rinse for a period 2 week. After initial clinical(plaque index, gingival index, probing pocket depth), microbiological and BANA tests were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. Clinical indices were measured, microbial parameters and BANA test were reassessed 1, 2 and 4 weeks after treatment. The results were as follows : 1. Plaque index, gingival index and pocket depth in chlorhexidine rinse group and control group were not significantly reduce during all weeks when compared chlorhexidine rinse group with control groups. Plaque index in chlorhexidine rinse group and control group were siginificantly reduced at 1, 2, 4weeks(P<0.05), gingival index and pocket depth wee ignificantly reduced at 2, 4weeks in both groups(P<0.05). 2. Perecntage of cocci and motile rods was significantly changed at 1, 2, 4weeks in chlorhexidine rinse group(P<0.05), control group was significantly changed at 4weeks in control group(P<0.05), intergroup difference was significantly at 2weeks in cocci and 4weeks in motile rods(P<0.05). 3. Percentage of non-motile rods in all group were not significantly changed when compared with those of baseline. 4. Percentage of spirochetes was significantly reduced at 4 week(P<0.05), control group was not significantly reduced during all weeks. 5. BANA test scores was significantly reduced during all weeks in chlorhexidine rinse group(P<0.05), control group was not significantly reduced during all weeks. The result showed that clinical and microbiological effect following scaling, root palning and chlorhexidine on periodontal disease.
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골절부위에 생긴 혈종의 골막하 이식이 골형성에 미치는 영향에 관한 실험적 연구 - 골스캔 소견을 중심으로 -
이명철(Myung Chul Lee),양승오(Seoung Oh Yang),강흥식(Hung Sik Kang),장기현(Kee Hyun Chang),구경회(Kyung Hoi Koo),성상철(Sang Chul Seung),박인애(In Ae Park) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1
N/A It has been reported that hematoma is one of the most crucial factors in fracture healing since callus formation is disturbed by washing out the hematoma near a fracture site. However, it is not clear why the hamatoma is important and how it plays a role during the fracture healing. In order to investigate the role of hematoma in the process of fracture healing, the osteogenic potential by subperiosteal transplantation have been studied. Experimental fractures by operation were made at the mid-shaft of the tibia in New Zealand white rabbits. Removal of hematoma at the fracture site was done after 2 and 3 days from experimental fracture, and the removed hematoma was transplanted into the subperiosteal area at the mid-shaft of the ulna of each rabbit. As control gorups, we have performed 3 different procedures 1) the hematoma was transplanted into the muscular layers at the thigh and forearm; 2) autologous blood clots were transplanted into the subperiosteal area of the ulna; and 3) sham operation without a transplantation into the subperiosteal area. After transplantation, seria bone scintigraphy and simple radiography were performed at 4 days, 1 week, and 2 weeks to detect an abnormality. The results of bone scintigraphy were positive in 5 of 6 experimental group. However, a11 in three control groups were negative. Histological observation of the positive bone revealed new bone formation with trabeculation. These results suggest the hematoma in fracture site has osteogenic potential in the subperiosteal area which can be demonstrable by bone scintigraphy and histologic findings. Therefore, it is considered that hematoma of the fracture site plays an important role in the process of fracture healing. Further biochemical investigation using various experimentaI models is mandatory to apply this preliminary result to the treatment of clinical delayed union or nonunion.
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복부농양진단을 위한 Indium - 111 표지백혈구스캔
김병태(Byung Tae Kim),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),이경수(Kyung Soo Lee),김명준(Myung Joon Kim),최형식(Hyung Shik Choi),양승오(Seung O Yang),이재훈(Jae Hoon Lee),최창순(Chang Soon Choi),김택규(Ta 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1
N/A Detection of deep-seated abscesses is sometimes difficult with ultrasonogrpahy or computed tomography alone. Indium-111-labeled leukocyte has widely used in the localization of abscesses after introduction by Segal and Thakur in 1976. But there are some difficulties in using indium- 111-oxine in our country because of hardness to get the radiopharmaceutical timely and long time for labeling leukocytes. So we performed the indium, 111-labeled leukocyte scan for establishment of the labeling procedure and clinical application. We labeled the mixed leukocytes from 36 mi of patient's blood using 4 mi of ACD solution, 7 ml of 6% hydroxyethyl starch solution (HESPANⓡ), 1 mCi of indium-111 oxine, 5 ml of normal saline and centrifuge. It took about 2 hours for the preparation of radiolabeled leukocytes and attention for contamination was needed. The average injected dose of labeled mixed leukocytes was 465 uCi. The average number of injected leukocytes was 2.5×108 and the labeling ratio was 57±13% (Table 2, Fig. 5). These number and ratio were sufficient for the localization of abscess. About twenty per cent of indium was labeled to red blood cells and platelets (Fig. 6) and the half-life of injected radiolabeled leukocytes was 8.3 hours. Scan was performed in 9 patients who were suspected to have abscesses clinically or radiologically. Three patients were positive, in one patient who had abscess close to lower lumbar vertebrae was surgically drained and another 2 positive cases did not show abscess clearly on computed tomography, so only antibiotics were administrated and treated successfully. The negative 6 patients were improved without specific treatment. In conclusion, the use of indium-111 oxine labeled leukocytes for localization of abscesses were very specific and helpful in the decision of treatment considering its relatively simple labeling method, and could be easily performed providing timely supply of the radiopharmaceutical.
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양승오,신형식 원광대학교 치의학연구소 1992 圓光齒醫學 Vol.2 No.2
Many studies of gingivitis in pregnancy have been reported over the years and many different determinations to it's cause and frequency have been resulted. 240 Korean pregnant women were selected, who were in pregnancy from 2 to 10 months visited Wonkwang university hospital and other maternity clinic at Iri city in 1992. The periodontal conditions were assessed according to the community periodontal index of treatment needs(CPITN). The aim of this survey was to obtian information which is necessary for the planning of preventive programs of periodontal disease for pregnant women. The percentage of pregnant women having pocket depth, plaque index, gingival index and periodontal index was higher with the month of pregnancy, reached a maximum of third trimester 03rd trimester, posterior teeth, periodontal index -1.19 0.70). The difference of each age group in pregnant women was statistically not significant and comparing PD, PI, GI, PEI of facial and lingual surface, in lingual surface were higher surface. The result of CPITN that pregnant women had a healthy periodontal condition(34.17%), improving the personal oral hygiene(48.33%), need for professional cleansing of the teeth(10%), need for complex treatment(7.5%). The study has shown that gingival changes are more effective in local irritant than plasma level of sex-hormone and need oral hygiene instruction than professional treatment.
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TGF-β1이 Minocycline을 전처리한 사람 치주인대세포의 활성에 미치는 영향
양승오,신형식 원광대학교 치의학연구소 1996 圓光齒醫學 Vol.6 No.2
The initial events required for periodontal regeneration is the attachment, spreading, and proliferation of appropriated cells at the healing sites. These have been reported that minocycline stimulates the attachment of periodontal ligament cells, and also TGF-β1 enhances the proliferation of periodontal ligament cells. The purpose of the present study was to evaluate the effects of TGF-β1 on the cellular activity of minocycline-treated human periodontal ligament cells. Periodontal ligament cells were obtained from the explants of healthy periodontal ligaments of extracted 3rd molars or premolar teeth extracted from the patients for orthodontic treatment. The cells were cultured in minimal essential medium(α-MEM) supplemented with 10,000 units/㎖ penicillin, 10,000 ㎎/㎖ streptomycin and 10% FBS(fetal bovine serum) at 37℃ in a humidified atmosphere of 5% carbon dioxide and the 5th to the 8th passages of the cells were used. To evaluate the effect of minocycline on cell attachment, the cells were seeded at a cell density of 1.5×10^4 cells/well in 24-well culture plates and treated with 20㎍/㎖ and 100 ㎍/㎖, of minocycline for 1.5 h. After trypsinization, the cells were counted with hemocytometer and were taken photographs for observation of cellular morphology. To evaluate the effect of TGF-β1 on minocycline-pretreated periodontal ligament cells, the cells were seeded at a cell density of 1×10^4 cells/well in 24-well culture plates and treated with 20㎍/㎖ and 100㎍/㎖ of minocycline for 1.5 h. After incubation, 1 and 10ng/㎖ of rh-TGF-β1 were also added to the each well and incubated for I and 2 days, respectively. Then, MTT assay, DNA synthesis(^3H-thymidine assay), and protein and collagen assay(^3H-proline assay) were carried out. In the MTT assay, after 200ul MTT solution (concentration of 5㎎/㎖) were added to the each well of the 24-well plates and incubated for 3 hours, and 200ul DMSO were added so as to dissolve insoluble blue formazan crystals which was formed in incubated period. Then it read plates on a ELISA reader. For mitogenic assay, 1uCi/㎖ ^3H-thymidine was added to each well for the final 2 hours of the incubation periods. After labeling, the wells were washed 3 times with ice cold PBS and 4 times with 5% TCA to remove unincorporated label and precipitate the cellular DNA. DNA, with the incorporated 3H-thymidine, was solubilized with 500ul of 0.1% NaOH/0.1% SDS. A 250ul aliquot was removed from each well and placed in a scintillation vial with 4㎖ of scintillation cocktail. Using an liquid scintillation counter, counts per minute(CPM) were determined for each samples. 3uCi/ml ^3H-proline was added to each well for the final 4 hours of the incubation periods and total protein and percent collagen synthesis were carried out. The results indicate that minocycline treated group with l00㎍/㎖ concentration for 1.5 hours significantly increased than that of control in cell attachment, and cell process is also evident compared with that of control in cell morphology, and the cellular activity and DNA synthesis rate of cells treated minocycline and TGF-β1 significantly increased than that of control values, but were below to values of the TGF-β1 only treated group in MTT assay and ^3H-thymidine assay, and the total protein synthesis of minocycline and TGF-β1 treated group also significantly increased than that of control values, but the percent collagen synthesis of tested group significantly decreased to compared with control. On the above the findings, the tested group of minocycline and TGF-β1 did not increase the effect on the cell activity than TGF-β1 only tested group and the tested group of minocycline inhibited cell activity. This results indicate that minocycline was effective on cell attachment in early stage, but it is harmful to cell activity, that inhibitory effect of minocycline was compensated with stimulatory effect of TGF-β1.
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